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The map label for this gene is pmbA [H]

Identifier: 34498652

GI number: 34498652

Start: 3480938

End: 3482245

Strand: Reverse

Name: pmbA [H]

Synonym: CV_3197

Alternate gene names: 34498652

Gene position: 3482245-3480938 (Counterclockwise)

Preceding gene: 34498658

Following gene: 34498651

Centisome position: 73.29

GC content: 69.11

Gene sequence:

>1308_bases
CTGGGCGGCATCGCCGGCCGCGTGCTCGAGCTGGCGAGCCAGCAGGGCGCCAGCTCGGCCGAGGTCGACGTTTCCGAGGG
CGTCGGCCAGACCGTGAGCGTGCGCCTGTCCGAGGTGGAAACCATAGAGTACAACCAGGACAAGGGCGTCAGCGTCACCG
TCTATCTGGGCAAGAAAAAGGGGCATGCCAGCACGTCGGATTTCTCTGACGAGGCGCTGTCCGATACCGTGCGGGCCGCG
CTGGACATTGCCCGCTACACGGCGGAGGACGACTGCGCCGGCCTGGCGGATCCGCAACTGCTGGCCGACGATCTGCCGGA
CCTGGACCTGTTCCATCCCTGGCAGTTGCCGGTGGAAGAGGCGATCGAGCTGGCGCGCCGCTGCGAGGACGCCGCGCGCG
CGGTGGACGCCCGCATCCGCAACTCCGAGGGCGCCAGCGTGTCGGTGCAGGCCAACCAGTTCGTATACGCCAACAGCAAC
GGTTTCAGCGGCGGCTTCGCCGGCAGCCGCCACAGCTTGTCGGCCGCCGTGGTGGCCGAGCAGGATGGCGTGATGCAGCG
CGACTACTGGTACTCCGCCGCCCGCCATCAGGATGATCTGGCGTCGGTGGAGGAGATCGGCCGCATCGCCGGCGAGCGCG
CGGTGCGCCGTCTGGGCGGGCGCCGGGTCAAGACCGGCCAGTACCCGGTGCTGTTCGAAGCGCCGGTGGCGATGTCGCTG
CTCGGGCATCTGGCCGCGGCGATCAGCGGCGGCAACCTGTACCGCAAGTCGTCCTTCCTGCTGGACGCGCTGGGCAAGCC
GGTGATGGCGTCCCGGGTGACGATAGACGAAGACCCGTTCCTGCTGCGCGGCCTCGCCAGCAGCGCCTTCGACAACGAGG
GCGTGGCGACCAAGGCGCGGCGGCTGGTGGACGGCGGCGTGCTGCAGGGCTACTTCCTCAGCAGCTACTCTGCGCGCAAG
CTGGGCATGCAGACCACCGGCAACGCCGGCGGCGCGCACAACCTGGTGGTCCATTCCACCGGCGAGAGTTTCGCCGAGGT
GCTGTCGCGCATGGGCAGCGGCCTGCTGGTGACCGAGCTGCTGGGCCAGGGCGTCAATACCGTCACCGGCGACTATTCGC
GCGGCGCGGCCGGCTTCTGGGTGGAAAACGGCGTGATCGCCTACCCGGTGGAGGAAATCACCATCGCCGGCAATCTGCGC
GACATGTTCGTCAATATCGAAGCCATCGCCGACGATACGCTGGACCGCGGCGGCCGCCGCATCGGCTCGGTGCTGGTGGG
CGGCATGATGGTGGCCGGCGAGGAGTGA

Upstream 100 bases:

>100_bases
GTTTTCAAGGCTGGTATGATACCGGAAAAGGCCGTCGGCCGTTTCCACTGTTCCGAAAGATACTATGGCAGATAAGACAT
TCAGTTTCAGTTCCGACACC

Downstream 100 bases:

>100_bases
GCGACAGCCTGTTCGCGCCGGACGACGGCCCGTGGACCGAGCGGCTGTCGGCCGGCGCCGCCTTGCTGCACGGCTGGGCC
TGCGCCGGCGACGCGGAGCT

Product: pmbA protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MGGIAGRVLELASQQGASSAEVDVSEGVGQTVSVRLSEVETIEYNQDKGVSVTVYLGKKKGHASTSDFSDEALSDTVRAA
LDIARYTAEDDCAGLADPQLLADDLPDLDLFHPWQLPVEEAIELARRCEDAARAVDARIRNSEGASVSVQANQFVYANSN
GFSGGFAGSRHSLSAAVVAEQDGVMQRDYWYSAARHQDDLASVEEIGRIAGERAVRRLGGRRVKTGQYPVLFEAPVAMSL
LGHLAAAISGGNLYRKSSFLLDALGKPVMASRVTIDEDPFLLRGLASSAFDNEGVATKARRLVDGGVLQGYFLSSYSARK
LGMQTTGNAGGAHNLVVHSTGESFAEVLSRMGSGLLVTELLGQGVNTVTGDYSRGAAGFWVENGVIAYPVEEITIAGNLR
DMFVNIEAIADDTLDRGGRRIGSVLVGGMMVAGEE

Sequences:

>Translated_435_residues
MGGIAGRVLELASQQGASSAEVDVSEGVGQTVSVRLSEVETIEYNQDKGVSVTVYLGKKKGHASTSDFSDEALSDTVRAA
LDIARYTAEDDCAGLADPQLLADDLPDLDLFHPWQLPVEEAIELARRCEDAARAVDARIRNSEGASVSVQANQFVYANSN
GFSGGFAGSRHSLSAAVVAEQDGVMQRDYWYSAARHQDDLASVEEIGRIAGERAVRRLGGRRVKTGQYPVLFEAPVAMSL
LGHLAAAISGGNLYRKSSFLLDALGKPVMASRVTIDEDPFLLRGLASSAFDNEGVATKARRLVDGGVLQGYFLSSYSARK
LGMQTTGNAGGAHNLVVHSTGESFAEVLSRMGSGLLVTELLGQGVNTVTGDYSRGAAGFWVENGVIAYPVEEITIAGNLR
DMFVNIEAIADDTLDRGGRRIGSVLVGGMMVAGEE
>Mature_434_residues
GGIAGRVLELASQQGASSAEVDVSEGVGQTVSVRLSEVETIEYNQDKGVSVTVYLGKKKGHASTSDFSDEALSDTVRAAL
DIARYTAEDDCAGLADPQLLADDLPDLDLFHPWQLPVEEAIELARRCEDAARAVDARIRNSEGASVSVQANQFVYANSNG
FSGGFAGSRHSLSAAVVAEQDGVMQRDYWYSAARHQDDLASVEEIGRIAGERAVRRLGGRRVKTGQYPVLFEAPVAMSLL
GHLAAAISGGNLYRKSSFLLDALGKPVMASRVTIDEDPFLLRGLASSAFDNEGVATKARRLVDGGVLQGYFLSSYSARKL
GMQTTGNAGGAHNLVVHSTGESFAEVLSRMGSGLLVTELLGQGVNTVTGDYSRGAAGFWVENGVIAYPVEEITIAGNLRD
MFVNIEAIADDTLDRGGRRIGSVLVGGMMVAGEE

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=426, Percent_Identity=51.8779342723005, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1789640, Length=397, Percent_Identity=24.1813602015113, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 46057; Mature: 45926

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGIAGRVLELASQQGASSAEVDVSEGVGQTVSVRLSEVETIEYNQDKGVSVTVYLGKKK
CCCHHHHHHHHHHHCCCCCCCEEHHHCCCCEEEEEEEEEEEEEECCCCCCEEEEEEECCC
GHASTSDFSDEALSDTVRAALDIARYTAEDDCAGLADPQLLADDLPDLDLFHPWQLPVEE
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHH
AIELARRCEDAARAVDARIRNSEGASVSVQANQFVYANSNGFSGGFAGSRHSLSAAVVAE
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECEEEEECCCCCCCCCCCCCCCCEEEEEEC
QDGVMQRDYWYSAARHQDDLASVEEIGRIAGERAVRRLGGRRVKTGQYPVLFEAPVAMSL
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECHHHHHH
LGHLAAAISGGNLYRKSSFLLDALGKPVMASRVTIDEDPFLLRGLASSAFDNEGVATKAR
HHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCHHHHHH
RLVDGGVLQGYFLSSYSARKLGMQTTGNAGGAHNLVVHSTGESFAEVLSRMGSGLLVTEL
HHHCCCCHHHHHHCCCCHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEHHHH
LGQGVNTVTGDYSRGAAGFWVENGVIAYPVEEITIAGNLRDMFVNIEAIADDTLDRGGRR
HCCCCCCCCCCCCCCCCCEEEECCEEEECCEEEEEECCCEEHEEEEHHHHHHHHHHHHHH
IGSVLVGGMMVAGEE
HHHHHHCCEEEECCC
>Mature Secondary Structure 
GGIAGRVLELASQQGASSAEVDVSEGVGQTVSVRLSEVETIEYNQDKGVSVTVYLGKKK
CCHHHHHHHHHHHCCCCCCCEEHHHCCCCEEEEEEEEEEEEEECCCCCCEEEEEEECCC
GHASTSDFSDEALSDTVRAALDIARYTAEDDCAGLADPQLLADDLPDLDLFHPWQLPVEE
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHH
AIELARRCEDAARAVDARIRNSEGASVSVQANQFVYANSNGFSGGFAGSRHSLSAAVVAE
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECEEEEECCCCCCCCCCCCCCCCEEEEEEC
QDGVMQRDYWYSAARHQDDLASVEEIGRIAGERAVRRLGGRRVKTGQYPVLFEAPVAMSL
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECHHHHHH
LGHLAAAISGGNLYRKSSFLLDALGKPVMASRVTIDEDPFLLRGLASSAFDNEGVATKAR
HHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCHHHHHH
RLVDGGVLQGYFLSSYSARKLGMQTTGNAGGAHNLVVHSTGESFAEVLSRMGSGLLVTEL
HHHCCCCHHHHHHCCCCHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEHHHH
LGQGVNTVTGDYSRGAAGFWVENGVIAYPVEEITIAGNLRDMFVNIEAIADDTLDRGGRR
HCCCCCCCCCCCCCCCCCEEEECCEEEECCEEEEEECCCEEHEEEEHHHHHHHHHHHHHH
IGSVLVGGMMVAGEE
HHHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]