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The map label for this gene is ybgJ [H]

Identifier: 34498625

GI number: 34498625

Start: 3453790

End: 3454452

Strand: Direct

Name: ybgJ [H]

Synonym: CV_3170

Alternate gene names: 34498625

Gene position: 3453790-3454452 (Clockwise)

Preceding gene: 34498623

Following gene: 34498627

Centisome position: 72.69

GC content: 70.14

Gene sequence:

>663_bases
ATGTCATCCGAATCCCACATTCCGCCGGCCGTTCACTGGCTCGGCAACCAGGCGCTGGTGCTGGAGCTGCCCCCCCCGGC
GACGCTGGACTGCCAGCGCCGAATCTGGCGATTGGCCGAACGGCTGCGCGCCGACGCCGACTTCCGCGAGGTCATTCCCG
GCATGAACAACCTGACGCTGGAGCTCCGGCCAGGCGCGAACGCCGCCGCCCTGCCCTCGCGGCTGCGCAAGCTGTGGCAG
TTCACGGAAGGCATCGTCGAAGACGGCCGCGTCGTGGATATCCCGGTCAGCTACGGCGGCCGTCACGGCCCGGACCTGGA
AGCCGTCGCCCGGCATTGCGGCCTGGCGCCCGACGAGGTCGTCGCCCGCCATGCGGCGGGCCGCTATACTGTGTTCTTCC
TGGGTTTCCAACCCGGCTTCGCCTACCTGGGAGGCCTGGATCCGGCTTTGTCCGCGCCGCGCCGCGCGACGCCCAGGCAA
AAAGTGCCGGCCGGCTCCGTCGCGATAGGCGGCAGCCAGACCGGCATTTATCCGACCGCCTCGCCCGGCGGCTGGCAAAT
CATCGGACGCAGCGACGCCGCGCTGTTCGATCCTTCGCGACAGCCGCCCTGCCCGCTGCGGCCCGGCGATATCGTGCGCT
TCGTACCGATGGAGGACGCCTGA

Upstream 100 bases:

>100_bases
AGGTAACGATAAATTGACGAGAAATTCTCGACATTGCATCATCGCCACCATCCAACCCATTATCATATCCATATTCCCGG
ATCACGCCATCCCGTACGCA

Downstream 100 bases:

>100_bases
TGCTGGAGGTGATCAAGGCCGGCCCACAGACCACGGTGCAGGACCTGGGCCGTTTCGGCGTCCGCCATCTGGGCGTGTGC
CGCGCCGGCACGATGGACGC

Product: carboxylase

Products: CO2; NH3

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSSESHIPPAVHWLGNQALVLELPPPATLDCQRRIWRLAERLRADADFREVIPGMNNLTLELRPGANAAALPSRLRKLWQ
FTEGIVEDGRVVDIPVSYGGRHGPDLEAVARHCGLAPDEVVARHAAGRYTVFFLGFQPGFAYLGGLDPALSAPRRATPRQ
KVPAGSVAIGGSQTGIYPTASPGGWQIIGRSDAALFDPSRQPPCPLRPGDIVRFVPMEDA

Sequences:

>Translated_220_residues
MSSESHIPPAVHWLGNQALVLELPPPATLDCQRRIWRLAERLRADADFREVIPGMNNLTLELRPGANAAALPSRLRKLWQ
FTEGIVEDGRVVDIPVSYGGRHGPDLEAVARHCGLAPDEVVARHAAGRYTVFFLGFQPGFAYLGGLDPALSAPRRATPRQ
KVPAGSVAIGGSQTGIYPTASPGGWQIIGRSDAALFDPSRQPPCPLRPGDIVRFVPMEDA
>Mature_219_residues
SSESHIPPAVHWLGNQALVLELPPPATLDCQRRIWRLAERLRADADFREVIPGMNNLTLELRPGANAAALPSRLRKLWQF
TEGIVEDGRVVDIPVSYGGRHGPDLEAVARHCGLAPDEVVARHAAGRYTVFFLGFQPGFAYLGGLDPALSAPRRATPRQK
VPAGSVAIGGSQTGIYPTASPGGWQIIGRSDAALFDPSRQPPCPLRPGDIVRFVPMEDA

Specific function: Unknown

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis ycsJ and yeast urea amidolyase (DUR1,2) [H]

Homologues:

Organism=Escherichia coli, GI1786929, Length=209, Percent_Identity=54.5454545454545, Blast_Score=225, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: 3.5.1.54

Molecular weight: Translated: 23766; Mature: 23635

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSESHIPPAVHWLGNQALVLELPPPATLDCQRRIWRLAERLRADADFREVIPGMNNLTL
CCCCCCCCHHHEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEE
ELRPGANAAALPSRLRKLWQFTEGIVEDGRVVDIPVSYGGRHGPDLEAVARHCGLAPDEV
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHCCCCHHHH
VARHAAGRYTVFFLGFQPGFAYLGGLDPALSAPRRATPRQKVPAGSVAIGGSQTGIYPTA
HHHHCCCCEEEEEEECCCCHHHHCCCCHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCC
SPGGWQIIGRSDAALFDPSRQPPCPLRPGDIVRFVPMEDA
CCCCEEEEECCCCEEECCCCCCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure 
SSESHIPPAVHWLGNQALVLELPPPATLDCQRRIWRLAERLRADADFREVIPGMNNLTL
CCCCCCCHHHEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEE
ELRPGANAAALPSRLRKLWQFTEGIVEDGRVVDIPVSYGGRHGPDLEAVARHCGLAPDEV
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHCCCCHHHH
VARHAAGRYTVFFLGFQPGFAYLGGLDPALSAPRRATPRQKVPAGSVAIGGSQTGIYPTA
HHHHCCCCEEEEEEECCCCHHHHCCCCHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCC
SPGGWQIIGRSDAALFDPSRQPPCPLRPGDIVRFVPMEDA
CCCCEEEEECCCCEEECCCCCCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: urea-1-carboxylate; H2O

Specific reaction: urea-1-carboxylate + H2O = 2 CO2 + 2 NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]