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The map label for this gene is tbpA [H]

Identifier: 34498619

GI number: 34498619

Start: 3447322

End: 3448338

Strand: Reverse

Name: tbpA [H]

Synonym: CV_3164

Alternate gene names: 34498619

Gene position: 3448338-3447322 (Counterclockwise)

Preceding gene: 34498622

Following gene: 34498618

Centisome position: 72.58

GC content: 66.76

Gene sequence:

>1017_bases
ATGAAACTGTTCCCCAAAATTGCCGCCGCCGCCTTGAGCCTGGCTGGACTGTCCGCCCAGGCCGCCGAACTGCGCGTGCT
TGCGCACAGTTCGTTCAGCGTCGACAAGCCGCTGCTCGCCGCCTTCGAGAAACAGACCGGCGCCAAGGTGTCCATCATCA
AGGCCGGCGACGCCGGCGAGATGGTCAACAAGCTGATACTGACCAAGGGCAGCCCGATCGCCGACGTGGTGTTCGGCATC
GACAACAGCCTGATCGGCAAGGCCGAGCAGGCCGGCGCGCTCGCGCCGCTGCCGGCGGAACTGGCCAAGGGCGGCAAGGT
GGGACTGCCGGGCGCGGCCGCCGTCGACTACGGTTACGTGACGCTGAACTACGACAAGGCTTGGTTCGCCAAGAACAAGC
TGCCGTTGCCCAAGACCCTGAACGACCTGGCGCTGCCGGCCTACAAGAACCTGCTGGTGGTGGAAAACCCCGCCACCTCC
AGTCCGGGCCTCGCCTTCCTGTCCGCCACCATCCACGCGCTGGGCGAGGACAAGGCCTTCGCGCTGTGGGGCAAGCTGCG
CGACAACGGCCTGAAGGTCAGCAAGGGCTGGACCGAAGCCTACTACACCGATTTCTCCAAGAACGGCGGCGGTCGGCCCA
TCGTGGTCAGCTACGCCACCAGTCCGGCGGCCGAGGTGTTCTACAGCAAGGACAAGCCGGCGGACGCGCCGACCGGCAAT
CTGTTGCTGCCGGGCACGGTGTTCCGCCAGGTGGAGGGCGCGGCGCTGGTCAAGGGCGGCAAGGAGCCCAAGCTCGCCCA
GCAGTTCATCGCCTTCCTGCGCTCGGACGCGGTGCAGAAGGACATTCCGACCCGGATGTGGATGTATCCGGCGATGGACA
ATATTCCGCTGGACCCGGTGTACAAGCATGTCGAGCAGCCGGCCGCGCATGACACCCCCGACAGCGCGACGCTGGCGGCC
AAGCAGCGCGCCTGGGTCGGCCGCTGGACGCGGGTGGTGCTGAAGTCCGGCCAGTGA

Upstream 100 bases:

>100_bases
AGAACCTGATCTGGATCATGCCAGCGGAGGGAGCGTGATGAAGCGGAATCTCGTCCGCCTTGCCTGCTTGCTCCGTGGCC
GCATCGCACGGAGATTGTCC

Downstream 100 bases:

>100_bases
GCGACGATGACTGGCCCGGCATCGCCTCTGTTATGATCCGGGCATGTTTGTTTGGAAGCTCATTCATGATCGACGACATC
ACCAGCCCCCGCCTGCTCCT

Product: thiamin ABC transporter substrate-binding protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKLFPKIAAAALSLAGLSAQAAELRVLAHSSFSVDKPLLAAFEKQTGAKVSIIKAGDAGEMVNKLILTKGSPIADVVFGI
DNSLIGKAEQAGALAPLPAELAKGGKVGLPGAAAVDYGYVTLNYDKAWFAKNKLPLPKTLNDLALPAYKNLLVVENPATS
SPGLAFLSATIHALGEDKAFALWGKLRDNGLKVSKGWTEAYYTDFSKNGGGRPIVVSYATSPAAEVFYSKDKPADAPTGN
LLLPGTVFRQVEGAALVKGGKEPKLAQQFIAFLRSDAVQKDIPTRMWMYPAMDNIPLDPVYKHVEQPAAHDTPDSATLAA
KQRAWVGRWTRVVLKSGQ

Sequences:

>Translated_338_residues
MKLFPKIAAAALSLAGLSAQAAELRVLAHSSFSVDKPLLAAFEKQTGAKVSIIKAGDAGEMVNKLILTKGSPIADVVFGI
DNSLIGKAEQAGALAPLPAELAKGGKVGLPGAAAVDYGYVTLNYDKAWFAKNKLPLPKTLNDLALPAYKNLLVVENPATS
SPGLAFLSATIHALGEDKAFALWGKLRDNGLKVSKGWTEAYYTDFSKNGGGRPIVVSYATSPAAEVFYSKDKPADAPTGN
LLLPGTVFRQVEGAALVKGGKEPKLAQQFIAFLRSDAVQKDIPTRMWMYPAMDNIPLDPVYKHVEQPAAHDTPDSATLAA
KQRAWVGRWTRVVLKSGQ
>Mature_338_residues
MKLFPKIAAAALSLAGLSAQAAELRVLAHSSFSVDKPLLAAFEKQTGAKVSIIKAGDAGEMVNKLILTKGSPIADVVFGI
DNSLIGKAEQAGALAPLPAELAKGGKVGLPGAAAVDYGYVTLNYDKAWFAKNKLPLPKTLNDLALPAYKNLLVVENPATS
SPGLAFLSATIHALGEDKAFALWGKLRDNGLKVSKGWTEAYYTDFSKNGGGRPIVVSYATSPAAEVFYSKDKPADAPTGN
LLLPGTVFRQVEGAALVKGGKEPKLAQQFIAFLRSDAVQKDIPTRMWMYPAMDNIPLDPVYKHVEQPAAHDTPDSATLAA
KQRAWVGRWTRVVLKSGQ

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=324, Percent_Identity=33.0246913580247, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 35882; Mature: 35882

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFPKIAAAALSLAGLSAQAAELRVLAHSSFSVDKPLLAAFEKQTGAKVSIIKAGDAGE
CCCCHHHHHHHHHHHCCCCHHHHEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCHHH
MVNKLILTKGSPIADVVFGIDNSLIGKAEQAGALAPLPAELAKGGKVGLPGAAAVDYGYV
HHHHHHHCCCCCHHHHEECCCCHHHCCHHHCCCCCCCCHHHHCCCCCCCCCCCEEEEEEE
TLNYDKAWFAKNKLPLPKTLNDLALPAYKNLLVVENPATSSPGLAFLSATIHALGEDKAF
EEECCHHEECCCCCCCCCCHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEE
ALWGKLRDNGLKVSKGWTEAYYTDFSKNGGGRPIVVSYATSPAAEVFYSKDKPADAPTGN
EEEEECCCCCEEEECCCCHHHHCCCCCCCCCCEEEEEECCCCHHHHEECCCCCCCCCCCC
LLLPGTVFRQVEGAALVKGGKEPKLAQQFIAFLRSDAVQKDIPTRMWMYPAMDNIPLDPV
EEECHHHHHHCCCHHEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHH
YKHVEQPAAHDTPDSATLAAKQRAWVGRWTRVVLKSGQ
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLFPKIAAAALSLAGLSAQAAELRVLAHSSFSVDKPLLAAFEKQTGAKVSIIKAGDAGE
CCCCHHHHHHHHHHHCCCCHHHHEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCHHH
MVNKLILTKGSPIADVVFGIDNSLIGKAEQAGALAPLPAELAKGGKVGLPGAAAVDYGYV
HHHHHHHCCCCCHHHHEECCCCHHHCCHHHCCCCCCCCHHHHCCCCCCCCCCCEEEEEEE
TLNYDKAWFAKNKLPLPKTLNDLALPAYKNLLVVENPATSSPGLAFLSATIHALGEDKAF
EEECCHHEECCCCCCCCCCHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEE
ALWGKLRDNGLKVSKGWTEAYYTDFSKNGGGRPIVVSYATSPAAEVFYSKDKPADAPTGN
EEEEECCCCCEEEECCCCHHHHCCCCCCCCCCEEEEEECCCCHHHHEECCCCCCCCCCCC
LLLPGTVFRQVEGAALVKGGKEPKLAQQFIAFLRSDAVQKDIPTRMWMYPAMDNIPLDPV
EEECHHHHHHCCCHHEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHH
YKHVEQPAAHDTPDSATLAAKQRAWVGRWTRVVLKSGQ
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]