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The map label for this gene is fliM [H]

Identifier: 34498585

GI number: 34498585

Start: 3414517

End: 3415521

Strand: Reverse

Name: fliM [H]

Synonym: CV_3130

Alternate gene names: 34498585

Gene position: 3415521-3414517 (Counterclockwise)

Preceding gene: 34498586

Following gene: 34498584

Centisome position: 71.89

GC content: 62.39

Gene sequence:

>1005_bases
ATGGGCGACGATATTCTGTCCCAGGAAGAGGTAGACGCGCTATTACGAGGCGTCTCCGGAGAGGACGAAGATGATGACGG
CGGGCAGGATGCCCAGGGTGTCCGCGGCTATGACATCGGCCGGCAGGAGCGGATTGTCCGCGGCCGGATGCCGACGCTGG
AGATCATCAACGAACGCTTCGCGCGCAACCTGCGCATCGGCTTGTTCAACTTCCTGCGCCGCAACGCGGAAATCTCCGTG
GGGCCGGTGCGGGTGCAGAAGTACAGCGAATTCATCCGCAACCTGGTGGTGCCGACCAACCTGAACCTGGTTCACGTGAA
GCCCTTGCGCGGCACCGGCCTGCTGATTTTCGACCCGGACCTGGTGTTCCTGATCGTCGACAACCTGTTCGGCAGCGACG
GACGCTACCATGTGCGCGTAGAGGGCCGCGACTTCACGCCGACCGAGCAGCGCATCATCCACCGGCTGCTGGAAGTGGTG
TTCACCGAATGCCAGAAGGCCTGGGAGCCGGTGTATCCGATAGAGTTCGTCTATCTGCGCTCGGAGATGAACACCCAGTT
CGCCAACATCGCCACGCCCACCGAAGTGGTGGTGGCGATGACCTTCCATATCGAACTGGGCGCCGGCGGCGGCGACTTTC
ACATCTGTCTGCCGTATTCTATGGTGGAGCCGATCCGCGATCTGCTTTCCAGCACCATGCAGGCCGACCGCACCGAGGTG
GACAACCGGTGGGTCAACCTGATGACCCACCAGGTGCAGGCGGCGGAGGTGGAGCTGGTGGCCAGCCTGGCGCACGCCAA
GGTCACGCTGGGGCAGATCCTGAATCTGAAGAACGGCGACGTGGTGATGATAGAAATACCCGAGCGGGTGGAGGCCGACG
TGTCCGGCATCCCGGTGTTCGAAGCCAGTTACGGCACCGTGCAGGGCCGATACGCGCTGAAGGTGGAAAAGATACTGGCG
GGGACCAACGAGTTCCTGGAGTCCCCCGGAGAGCATGAGCAATGA

Upstream 100 bases:

>100_bases
GTCAAACAGATCATCAACGAGGCGATGGACGCGGGCGAAGAGCCCGTGGTCGAAAGCGTCCTCTTCACTTCCTTCATCAT
CCAGAAGCAGTAGGCGCGCC

Downstream 100 bases:

>100_bases
GCGAAGAAGAATTGCAGGGCGGCGCGGCCGGCGCCGAAGAGGAGGTCTCGATGGACGACTGGGCCGCCGCGATGGCGGAG
CAGGAAACTGTCGAAACCCA

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MGDDILSQEEVDALLRGVSGEDEDDDGGQDAQGVRGYDIGRQERIVRGRMPTLEIINERFARNLRIGLFNFLRRNAEISV
GPVRVQKYSEFIRNLVVPTNLNLVHVKPLRGTGLLIFDPDLVFLIVDNLFGSDGRYHVRVEGRDFTPTEQRIIHRLLEVV
FTECQKAWEPVYPIEFVYLRSEMNTQFANIATPTEVVVAMTFHIELGAGGGDFHICLPYSMVEPIRDLLSSTMQADRTEV
DNRWVNLMTHQVQAAEVELVASLAHAKVTLGQILNLKNGDVVMIEIPERVEADVSGIPVFEASYGTVQGRYALKVEKILA
GTNEFLESPGEHEQ

Sequences:

>Translated_334_residues
MGDDILSQEEVDALLRGVSGEDEDDDGGQDAQGVRGYDIGRQERIVRGRMPTLEIINERFARNLRIGLFNFLRRNAEISV
GPVRVQKYSEFIRNLVVPTNLNLVHVKPLRGTGLLIFDPDLVFLIVDNLFGSDGRYHVRVEGRDFTPTEQRIIHRLLEVV
FTECQKAWEPVYPIEFVYLRSEMNTQFANIATPTEVVVAMTFHIELGAGGGDFHICLPYSMVEPIRDLLSSTMQADRTEV
DNRWVNLMTHQVQAAEVELVASLAHAKVTLGQILNLKNGDVVMIEIPERVEADVSGIPVFEASYGTVQGRYALKVEKILA
GTNEFLESPGEHEQ
>Mature_333_residues
GDDILSQEEVDALLRGVSGEDEDDDGGQDAQGVRGYDIGRQERIVRGRMPTLEIINERFARNLRIGLFNFLRRNAEISVG
PVRVQKYSEFIRNLVVPTNLNLVHVKPLRGTGLLIFDPDLVFLIVDNLFGSDGRYHVRVEGRDFTPTEQRIIHRLLEVVF
TECQKAWEPVYPIEFVYLRSEMNTQFANIATPTEVVVAMTFHIELGAGGGDFHICLPYSMVEPIRDLLSSTMQADRTEVD
NRWVNLMTHQVQAAEVELVASLAHAKVTLGQILNLKNGDVVMIEIPERVEADVSGIPVFEASYGTVQGRYALKVEKILAG
TNEFLESPGEHEQ

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=335, Percent_Identity=50.1492537313433, Blast_Score=337, Evalue=6e-94,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37548; Mature: 37417

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDDILSQEEVDALLRGVSGEDEDDDGGQDAQGVRGYDIGRQERIVRGRMPTLEIINERF
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHH
ARNLRIGLFNFLRRNAEISVGPVRVQKYSEFIRNLVVPTNLNLVHVKPLRGTGLLIFDPD
HHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECCC
LVFLIVDNLFGSDGRYHVRVEGRDFTPTEQRIIHRLLEVVFTECQKAWEPVYPIEFVYLR
EEEEEEEHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
SEMNTQFANIATPTEVVVAMTFHIELGAGGGDFHICLPYSMVEPIRDLLSSTMQADRTEV
CCCCCHHHCCCCCCCEEEEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
DNRWVNLMTHQVQAAEVELVASLAHAKVTLGQILNLKNGDVVMIEIPERVEADVSGIPVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHCCCCCCCEEE
EASYGTVQGRYALKVEKILAGTNEFLESPGEHEQ
ECCCCCCCCEEEEEHHHHHHCCHHHHCCCCCCCC
>Mature Secondary Structure 
GDDILSQEEVDALLRGVSGEDEDDDGGQDAQGVRGYDIGRQERIVRGRMPTLEIINERF
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHH
ARNLRIGLFNFLRRNAEISVGPVRVQKYSEFIRNLVVPTNLNLVHVKPLRGTGLLIFDPD
HHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECCC
LVFLIVDNLFGSDGRYHVRVEGRDFTPTEQRIIHRLLEVVFTECQKAWEPVYPIEFVYLR
EEEEEEEHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
SEMNTQFANIATPTEVVVAMTFHIELGAGGGDFHICLPYSMVEPIRDLLSSTMQADRTEV
CCCCCHHHCCCCCCCEEEEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
DNRWVNLMTHQVQAAEVELVASLAHAKVTLGQILNLKNGDVVMIEIPERVEADVSGIPVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHCCCCCCCEEE
EASYGTVQGRYALKVEKILAGTNEFLESPGEHEQ
ECCCCCCCCEEEEEHHHHHHCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3519573; 9097040; 9278503; 2651416 [H]