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The map label for this gene is fliN [H]

Identifier: 34498584

GI number: 34498584

Start: 3414077

End: 3414520

Strand: Reverse

Name: fliN [H]

Synonym: CV_3129

Alternate gene names: 34498584

Gene position: 3414520-3414077 (Counterclockwise)

Preceding gene: 34498585

Following gene: 34498583

Centisome position: 71.87

GC content: 65.99

Gene sequence:

>444_bases
ATGAGCGAAGAAGAATTGCAGGGCGGCGCGGCCGGCGCCGAAGAGGAGGTCTCGATGGACGACTGGGCCGCCGCGATGGC
GGAGCAGGAAACTGTCGAAACCCAGCATGCGGTGGAGGCGGAGGCGGTTCCGGCCCAGGGCCTGTTCCAGGAGATCGGCG
CCGGGGCGGTGGCCGGCGGCCCTCCCAATCTGGACATGATCCTGGACATCCCGGTCCAGCTGACTGTCGAGCTGGGGCGC
ACCAAGATCGCCATTCGCAACCTGCTGCAACTGGCGCAGGGTTCGGTGGTGGAGCTGGACGGCATGGCCGGCGAGCCGAT
GGACGTGCTGGTCAACGGCTGTCTGATCGCCCAGGGCGAGGTGGTGGTGGTCAACGACAAGTTCGGCATTCGTCTGACCG
ACATCATCACCCCGGCAGAGCGGATACGGCGGCTGCAGAAATAA

Upstream 100 bases:

>100_bases
AAGCCAGTTACGGCACCGTGCAGGGCCGATACGCGCTGAAGGTGGAAAAGATACTGGCGGGGACCAACGAGTTCCTGGAG
TCCCCCGGAGAGCATGAGCA

Downstream 100 bases:

>100_bases
TGGACATGGGAAAACGCGTGAAAGTGCGCCTGGCGCTGCGGGGCGCACTTTGTTTTATGGCATCTTCGCCGCTGTGGGCG
GCGACCGCTACGGCTCCAGC

Product: flagellar motor switch protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MSEEELQGGAAGAEEEVSMDDWAAAMAEQETVETQHAVEAEAVPAQGLFQEIGAGAVAGGPPNLDMILDIPVQLTVELGR
TKIAIRNLLQLAQGSVVELDGMAGEPMDVLVNGCLIAQGEVVVVNDKFGIRLTDIITPAERIRRLQK

Sequences:

>Translated_147_residues
MSEEELQGGAAGAEEEVSMDDWAAAMAEQETVETQHAVEAEAVPAQGLFQEIGAGAVAGGPPNLDMILDIPVQLTVELGR
TKIAIRNLLQLAQGSVVELDGMAGEPMDVLVNGCLIAQGEVVVVNDKFGIRLTDIITPAERIRRLQK
>Mature_146_residues
SEEELQGGAAGAEEEVSMDDWAAAMAEQETVETQHAVEAEAVPAQGLFQEIGAGAVAGGPPNLDMILDIPVQLTVELGRT
KIAIRNLLQLAQGSVVELDGMAGEPMDVLVNGCLIAQGEVVVVNDKFGIRLTDIITPAERIRRLQK

Specific function: FliN is one of three proteins (FliG, FliN, FliM) that form a switch complex that is proposed to be located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor

COG id: COG1886

COG function: function code NU; Flagellar motor switch/type III secretory pathway protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliN/MopA/SpaO family [H]

Homologues:

Organism=Escherichia coli, GI1788256, Length=131, Percent_Identity=57.2519083969466, Blast_Score=157, Evalue=2e-40,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012826
- InterPro:   IPR001172
- InterPro:   IPR001543 [H]

Pfam domain/function: PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 15561; Mature: 15430

Theoretical pI: Translated: 3.88; Mature: 3.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEELQGGAAGAEEEVSMDDWAAAMAEQETVETQHAVEAEAVPAQGLFQEIGAGAVAGG
CCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
PPNLDMILDIPVQLTVELGRTKIAIRNLLQLAQGSVVELDGMAGEPMDVLVNGCLIAQGE
CCCCCEEEECCEEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCEEEECCC
VVVVNDKFGIRLTDIITPAERIRRLQK
EEEEECCCCEEEHHHCCHHHHHHHHCC
>Mature Secondary Structure 
SEEELQGGAAGAEEEVSMDDWAAAMAEQETVETQHAVEAEAVPAQGLFQEIGAGAVAGG
CCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
PPNLDMILDIPVQLTVELGRTKIAIRNLLQLAQGSVVELDGMAGEPMDVLVNGCLIAQGE
CCCCCEEEECCEEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCEEEECCC
VVVVNDKFGIRLTDIITPAERIRRLQK
EEEEECCCCEEEHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2651416; 9097040; 9278503 [H]