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The map label for this gene is hisM2 [H]

Identifier: 34498542

GI number: 34498542

Start: 3367169

End: 3367834

Strand: Direct

Name: hisM2 [H]

Synonym: CV_3087

Alternate gene names: 34498542

Gene position: 3367169-3367834 (Clockwise)

Preceding gene: 34498541

Following gene: 34498543

Centisome position: 70.87

GC content: 67.42

Gene sequence:

>666_bases
ATGCTGTCCAGCACCGACGGTCTGATCCTGACGCTGGAGCTGCTGGCCCTGTCGCTGCTGCTGGGCCTGGCCATGGCGGT
TCCGCTGGCGGTGGCCCGCGTCTCCAGGAGGCGCGCGCTGTCCGGCGCCGTCTGGCTGTTCGGCTACGTGTTCCGCGGCA
CGCCGCTGTTGGTGCAGCTGTTCATCATCTACTACGGCCTGGCGCAATTCGACTGGGTGCGCGACAGCTGGGCCTGGCAT
TACCTGCAGGACGCCTATGTCTGCGCGATCCTGGCTTTCACGCTGAACACCGCCGCCTACACCACCGAAATCATCGCCGG
CCAGATCCGCGCCACCCACTGGGGCGAGATCGAGGCGGCGCGCTCGATGGGCATGAGCCACTGGCTGATGCTGCGCCGGA
TCGTGCTGCCTTCGGCGCTGCGCCGCGCGCTGCCCGCCTACAGCAACGAAGTGATCATGATGCTGCAAAGCACCGCGATC
GCCGGCCTGGTGACGCTGGCCGACCTCACCGGCGTGGCCCGGCGCATCTACAGCGAGAGCTATATGCCGTTCGAGCCCTT
CCTCACCGCCGCGCTGATCTACCTGGCCCTGACCTTCGCCCTGGTCTGGCTGATGAAGCGCGCCGAACGCCGCTATCTGG
CCTTCCTCGCCCCCCGCAAGCCATGA

Upstream 100 bases:

>100_bases
AACGCTATTCGATGGGCACCAAGGAGAGCGGCCTGTGATCGATCCCCAACTGATTATCGAACGGCTGCCTGAATTTTTCG
GCGCCCGTGACGGCGGCCCG

Downstream 100 bases:

>100_bases
GGCCGCACGGGCCGGCAACAATTTGTGCCGGCCCGCCGAAATAAATCGTACAATGCAGGGCAAGATAACGCCGCCATGCC
AGGCCAGCCTGACATGGCGG

Product: histidine transport system membrane protein M

Products: ADP; phosphate; L-histidine [Cytoplasm]; ADP; L-ornithine [Cytoplasm]; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MLSSTDGLILTLELLALSLLLGLAMAVPLAVARVSRRRALSGAVWLFGYVFRGTPLLVQLFIIYYGLAQFDWVRDSWAWH
YLQDAYVCAILAFTLNTAAYTTEIIAGQIRATHWGEIEAARSMGMSHWLMLRRIVLPSALRRALPAYSNEVIMMLQSTAI
AGLVTLADLTGVARRIYSESYMPFEPFLTAALIYLALTFALVWLMKRAERRYLAFLAPRKP

Sequences:

>Translated_221_residues
MLSSTDGLILTLELLALSLLLGLAMAVPLAVARVSRRRALSGAVWLFGYVFRGTPLLVQLFIIYYGLAQFDWVRDSWAWH
YLQDAYVCAILAFTLNTAAYTTEIIAGQIRATHWGEIEAARSMGMSHWLMLRRIVLPSALRRALPAYSNEVIMMLQSTAI
AGLVTLADLTGVARRIYSESYMPFEPFLTAALIYLALTFALVWLMKRAERRYLAFLAPRKP
>Mature_221_residues
MLSSTDGLILTLELLALSLLLGLAMAVPLAVARVSRRRALSGAVWLFGYVFRGTPLLVQLFIIYYGLAQFDWVRDSWAWH
YLQDAYVCAILAFTLNTAAYTTEIIAGQIRATHWGEIEAARSMGMSHWLMLRRIVLPSALRRALPAYSNEVIMMLQSTAI
AGLVTLADLTGVARRIYSESYMPFEPFLTAALIYLALTFALVWLMKRAERRYLAFLAPRKP

Specific function: Part of the binding-protein-dependent transport system for histidine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4160

COG function: function code E; ABC-type arginine/histidine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788645, Length=213, Percent_Identity=48.3568075117371, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1787086, Length=214, Percent_Identity=38.785046728972, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI1788226, Length=201, Percent_Identity=34.8258706467662, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787030, Length=207, Percent_Identity=30.4347826086957, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787087, Length=227, Percent_Identity=26.431718061674, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788646, Length=211, Percent_Identity=31.7535545023697, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786873, Length=207, Percent_Identity=28.0193236714976, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI87082239, Length=210, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1786874, Length=210, Percent_Identity=24.2857142857143, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI48994930, Length=122, Percent_Identity=27.0491803278689, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 24867; Mature: 24867

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSSTDGLILTLELLALSLLLGLAMAVPLAVARVSRRRALSGAVWLFGYVFRGTPLLVQL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FIIYYGLAQFDWVRDSWAWHYLQDAYVCAILAFTLNTAAYTTEIIAGQIRATHWGEIEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RSMGMSHWLMLRRIVLPSALRRALPAYSNEVIMMLQSTAIAGLVTLADLTGVARRIYSES
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YMPFEPFLTAALIYLALTFALVWLMKRAERRYLAFLAPRKP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSSTDGLILTLELLALSLLLGLAMAVPLAVARVSRRRALSGAVWLFGYVFRGTPLLVQL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FIIYYGLAQFDWVRDSWAWHYLQDAYVCAILAFTLNTAAYTTEIIAGQIRATHWGEIEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RSMGMSHWLMLRRIVLPSALRRALPAYSNEVIMMLQSTAIAGLVTLADLTGVARRIYSES
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YMPFEPFLTAALIYLALTFALVWLMKRAERRYLAFLAPRKP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O; ATP; L-ornithine [Periplasm]; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [P

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]