Click here to switch to the map view.

The map label for this gene is hisQ2 [H]

Identifier: 34498541

GI number: 34498541

Start: 3366420

End: 3367106

Strand: Direct

Name: hisQ2 [H]

Synonym: CV_3086

Alternate gene names: 34498541

Gene position: 3366420-3367106 (Clockwise)

Preceding gene: 34498540

Following gene: 34498542

Centisome position: 70.86

GC content: 64.34

Gene sequence:

>687_bases
ATGCTTCAAGGCTACCTTCCCAGCATTCTAGAAGGCGGTGTGCTGACCCTGAAACTGGCCGGCACCGCGCTGGTCGTGTC
CGTCGCGCTCGGCCTGGTCGGCGCGCTGTTCAAGTCCAGCCACTCGCGCCCGCTGACCCTGCTGGCAGAATTCTACTCCA
CCGTGGTGCGCGGCGTTCCGGACCTGGTGTGGATGTTCCTGCTGTTCTTCGGCGCCCAGATGGCTCTCAATGACCTCTGC
GGCTACTTCGGCTGGCAAAGCCCGGACATCGACCCGTTCGCCGCGGGCGTGGCCACGCTGGGCTTCATCTTCGGCGCCTA
CATGACCGAGACCTTCCGCGGCGCGATGATGGCCGTGCACAAGGGCCAGATGGAAGCCGGGCTCGCCTACGGCATGACGC
CGCTGCGCGTGTTCTTCCGCATCCAGCTGCCGCAGATGGTGCGCTATGCGCTGCCCAGCTTCTCCAACAACTGGCTGGTG
CTGGTGAAGTCCACCGCCCTGGTGTCTGTGATCGGCCTCAATGACGTGATGTACCGCGCCGATGCCGCCAAATCCGCCAC
CCAGCAGCCGTTCGACGTCTACATCGTCGTCGGCGCGTTGTTCCTCGTCGTCACCGGCGTGTCCAACCTGCTGCTCCACT
GGCTGGAGAAACGCTATTCGATGGGCACCAAGGAGAGCGGCCTGTGA

Upstream 100 bases:

>100_bases
GACATCTACGGCGGCTGATTCCTGAACTGATCCCACCCGATACGACCCGGCGCGCCGCCCGCGCGCCGGCGTCGGCAATC
CCTTTTTCCGAGATTGCTTC

Downstream 100 bases:

>100_bases
TCGATCCCCAACTGATTATCGAACGGCTGCCTGAATTTTTCGGCGCCCGTGACGGCGGCCCGATGCTGTCCAGCACCGAC
GGTCTGATCCTGACGCTGGA

Product: histidine transport system permease

Products: ADP; phosphate; L-histidine [Cytoplasm]; ADP; L-ornithine [Cytoplasm]; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MLQGYLPSILEGGVLTLKLAGTALVVSVALGLVGALFKSSHSRPLTLLAEFYSTVVRGVPDLVWMFLLFFGAQMALNDLC
GYFGWQSPDIDPFAAGVATLGFIFGAYMTETFRGAMMAVHKGQMEAGLAYGMTPLRVFFRIQLPQMVRYALPSFSNNWLV
LVKSTALVSVIGLNDVMYRADAAKSATQQPFDVYIVVGALFLVVTGVSNLLLHWLEKRYSMGTKESGL

Sequences:

>Translated_228_residues
MLQGYLPSILEGGVLTLKLAGTALVVSVALGLVGALFKSSHSRPLTLLAEFYSTVVRGVPDLVWMFLLFFGAQMALNDLC
GYFGWQSPDIDPFAAGVATLGFIFGAYMTETFRGAMMAVHKGQMEAGLAYGMTPLRVFFRIQLPQMVRYALPSFSNNWLV
LVKSTALVSVIGLNDVMYRADAAKSATQQPFDVYIVVGALFLVVTGVSNLLLHWLEKRYSMGTKESGL
>Mature_228_residues
MLQGYLPSILEGGVLTLKLAGTALVVSVALGLVGALFKSSHSRPLTLLAEFYSTVVRGVPDLVWMFLLFFGAQMALNDLC
GYFGWQSPDIDPFAAGVATLGFIFGAYMTETFRGAMMAVHKGQMEAGLAYGMTPLRVFFRIQLPQMVRYALPSFSNNWLV
LVKSTALVSVIGLNDVMYRADAAKSATQQPFDVYIVVGALFLVVTGVSNLLLHWLEKRYSMGTKESGL

Specific function: Part of the binding-protein-dependent transport system for histidine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4215

COG function: function code E; ABC-type arginine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788646, Length=228, Percent_Identity=51.7543859649123, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI1787087, Length=217, Percent_Identity=37.7880184331797, Blast_Score=147, Evalue=5e-37,
Organism=Escherichia coli, GI1787030, Length=216, Percent_Identity=29.6296296296296, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788226, Length=220, Percent_Identity=30.4545454545455, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1788645, Length=208, Percent_Identity=29.8076923076923, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786873, Length=218, Percent_Identity=30.2752293577982, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1787086, Length=216, Percent_Identity=28.7037037037037, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1786874, Length=218, Percent_Identity=23.8532110091743, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 24838; Mature: 24838

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQGYLPSILEGGVLTLKLAGTALVVSVALGLVGALFKSSHSRPLTLLAEFYSTVVRGVP
CCCCCCHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCH
DLVWMFLLFFGAQMALNDLCGYFGWQSPDIDPFAAGVATLGFIFGAYMTETFRGAMMAVH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGQMEAGLAYGMTPLRVFFRIQLPQMVRYALPSFSNNWLVLVKSTALVSVIGLNDVMYRA
HCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
DAAKSATQQPFDVYIVVGALFLVVTGVSNLLLHWLEKRYSMGTKESGL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLQGYLPSILEGGVLTLKLAGTALVVSVALGLVGALFKSSHSRPLTLLAEFYSTVVRGVP
CCCCCCHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCH
DLVWMFLLFFGAQMALNDLCGYFGWQSPDIDPFAAGVATLGFIFGAYMTETFRGAMMAVH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGQMEAGLAYGMTPLRVFFRIQLPQMVRYALPSFSNNWLVLVKSTALVSVIGLNDVMYRA
HCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
DAAKSATQQPFDVYIVVGALFLVVTGVSNLLLHWLEKRYSMGTKESGL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O; ATP; L-ornithine [Periplasm]; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [P

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]