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The map label for this gene is aotJ [H]

Identifier: 34498540

GI number: 34498540

Start: 3365573

End: 3366337

Strand: Direct

Name: aotJ [H]

Synonym: CV_3085

Alternate gene names: 34498540

Gene position: 3365573-3366337 (Clockwise)

Preceding gene: 34498537

Following gene: 34498541

Centisome position: 70.84

GC content: 61.96

Gene sequence:

>765_bases
ATGAAAAAAGCCCTGATCGCAAGCGCCGGCCTCGCCCTGCTCGCCTCCCTGTCCGCTCAAGCCGAAACCCTGCGCGTCGG
CGTGGATCTCAACTACCCGCCGTTCTCCAAGCAAGGCGCCGACGGCAAGCCGCAAGGCTTCGACATCGACATCGCCAACG
CGCTGTGCGCGGCGATGAAGGTCACTTGCCAGATCGTGCCGCAAGACTGGGACGGCCTGATCCCGGCGCTGAACGCCAAC
AAGTTCGACGTCATCATCTCGTCGATGCAGATCACGCCGGAACGCCAGAAGGCGGTGGACTTCTCGCACAAGTACTACAA
CATCGCCAGCCGCATGGTGGCCAAGGACGGCACCAAGGTCGACGCCGCCAGCCTGAAGGGCAAGAAGATCGGCGTGCTGC
GCGCGTCCACCCAGGAAAAGTTCGCCAAGGATTACTGGGGCAAGAACGGCGCCACCGTCGTGCCTTACGCCAAATCGCCC
GAATCCTTCCTGGACCTGAAGTCCGGCCGCGTCGACGCGGTGTTCGTCGACAGCGCCGTAGGCGAGCAAGAGTTCCTCAA
GACCACCCAGGCCAAGGGCTACAGCTTCGTCGGCCCCAACTACTCCGACGTCAAGTACTTCGGCCCCGGCTGCGGCATCG
CCGTCAAGAAAGGCAACAAGGCCTTGGTCGAGCGCCTGAACAAGGGCATCGACCAGATCCGCAAGGACGGTTCCTACAAG
AAAGTTCAGGACAAGTACTTCAGCTTCGACATCTACGGCGGCTGA

Upstream 100 bases:

>100_bases
CCCGGATCGGAACATTAACGTTGCCTCCGGTGCGGGGTTTACAAGCAGGCCGCCGGCGCGTCCCGCCAGCGGCCAACGAG
AGAGAAGTAGAAGCAACATC

Downstream 100 bases:

>100_bases
TTCCTGAACTGATCCCACCCGATACGACCCGGCGCGCCGCCCGCGCGCCGGCGTCGGCAATCCCTTTTTCCGAGATTGCT
TCATGCTTCAAGGCTACCTT

Product: arginine/ornithine ABC transporter substrate-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKKALIASAGLALLASLSAQAETLRVGVDLNYPPFSKQGADGKPQGFDIDIANALCAAMKVTCQIVPQDWDGLIPALNAN
KFDVIISSMQITPERQKAVDFSHKYYNIASRMVAKDGTKVDAASLKGKKIGVLRASTQEKFAKDYWGKNGATVVPYAKSP
ESFLDLKSGRVDAVFVDSAVGEQEFLKTTQAKGYSFVGPNYSDVKYFGPGCGIAVKKGNKALVERLNKGIDQIRKDGSYK
KVQDKYFSFDIYGG

Sequences:

>Translated_254_residues
MKKALIASAGLALLASLSAQAETLRVGVDLNYPPFSKQGADGKPQGFDIDIANALCAAMKVTCQIVPQDWDGLIPALNAN
KFDVIISSMQITPERQKAVDFSHKYYNIASRMVAKDGTKVDAASLKGKKIGVLRASTQEKFAKDYWGKNGATVVPYAKSP
ESFLDLKSGRVDAVFVDSAVGEQEFLKTTQAKGYSFVGPNYSDVKYFGPGCGIAVKKGNKALVERLNKGIDQIRKDGSYK
KVQDKYFSFDIYGG
>Mature_254_residues
MKKALIASAGLALLASLSAQAETLRVGVDLNYPPFSKQGADGKPQGFDIDIANALCAAMKVTCQIVPQDWDGLIPALNAN
KFDVIISSMQITPERQKAVDFSHKYYNIASRMVAKDGTKVDAASLKGKKIGVLRASTQEKFAKDYWGKNGATVVPYAKSP
ESFLDLKSGRVDAVFVDSAVGEQEFLKTTQAKGYSFVGPNYSDVKYFGPGCGIAVKKGNKALVERLNKGIDQIRKDGSYK
KVQDKYFSFDIYGG

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=262, Percent_Identity=42.3664122137405, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI1788648, Length=235, Percent_Identity=40.4255319148936, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1787085, Length=250, Percent_Identity=42, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1787088, Length=249, Percent_Identity=39.7590361445783, Blast_Score=177, Evalue=5e-46,
Organism=Escherichia coli, GI1788228, Length=266, Percent_Identity=31.203007518797, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1787031, Length=249, Percent_Identity=29.718875502008, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1786876, Length=224, Percent_Identity=26.7857142857143, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27529; Mature: 27529

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALIASAGLALLASLSAQAETLRVGVDLNYPPFSKQGADGKPQGFDIDIANALCAAMK
CCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHHH
VTCQIVPQDWDGLIPALNANKFDVIISSMQITPERQKAVDFSHKYYNIASRMVAKDGTKV
EEEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEE
DAASLKGKKIGVLRASTQEKFAKDYWGKNGATVVPYAKSPESFLDLKSGRVDAVFVDSAV
EHHHCCCCEEEEEECCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHCCCCEEEEEECCCC
GEQEFLKTTQAKGYSFVGPNYSDVKYFGPGCGIAVKKGNKALVERLNKGIDQIRKDGSYK
CHHHHHHHHHCCCEEECCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCCHH
KVQDKYFSFDIYGG
HHHHCEEEEEECCC
>Mature Secondary Structure
MKKALIASAGLALLASLSAQAETLRVGVDLNYPPFSKQGADGKPQGFDIDIANALCAAMK
CCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHHH
VTCQIVPQDWDGLIPALNANKFDVIISSMQITPERQKAVDFSHKYYNIASRMVAKDGTKV
EEEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEE
DAASLKGKKIGVLRASTQEKFAKDYWGKNGATVVPYAKSPESFLDLKSGRVDAVFVDSAV
EHHHCCCCEEEEEECCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHCCCCEEEEEECCCC
GEQEFLKTTQAKGYSFVGPNYSDVKYFGPGCGIAVKKGNKALVERLNKGIDQIRKDGSYK
CHHHHHHHHHCCCEEECCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCCHH
KVQDKYFSFDIYGG
HHHHCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]