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The map label for this gene is bsdA [H]

Identifier: 34498516

GI number: 34498516

Start: 3340378

End: 3341271

Strand: Reverse

Name: bsdA [H]

Synonym: CV_3061

Alternate gene names: 34498516

Gene position: 3341271-3340378 (Counterclockwise)

Preceding gene: 34498517

Following gene: 34498515

Centisome position: 70.33

GC content: 66.0

Gene sequence:

>894_bases
ATGGAAATCTACCAGTTGCGCACCTTCGTCACCGTGGCGCAGCAGGGCCATCTGACGCAGGCGGCCGAGCTGCTGCACCT
GTCGCAGCCGGCGGTGACCGCGCAGATCAAGGCCTTGGAAGAAGAAGTGGGCATGCCGCTGTTCGAGCGCAACGCCGGCG
GCGTCAGCCTCACCCGGGCCGGGCAGGAGCTGCTGCCTCAGGCTCAGGGCGTGCTGGCCGCGGCGCGCGACATCATCAAC
CACGCCAAGCAGCTGAAGGGACAGTTGTCCGGCCAGGCGCGCGTCGGCGTGACGCTGATGCCGGACATCCTGAAGCTGGG
GCCATGGGTGGCCGCGCTGGTGAGCGCGCACCCGTTGCTGGAGGTGCAGTTGACCCACGGCGTGTCGGTGGACGTGCTCA
ATCTGGTGCGCAAGAAGGAACTGGACGCCGGCTTCTACCTGGGCAAGAACCCTTATATGAACGTCAGCACCGTGCCCTTG
CAGACGCTGGATTTCCGCGTCGCGCTGCCGCCTGCCTGGGCGGGGCAGATGGCGTCGGGAAAGCTGAAGGATTTGGGCAA
GCTGCCCTGGGTGGGCATTTCGCAGTTTTCCAGCCTGTCCAAGATCACCGCGGAGTTGTGGCGCGAGATCAATATCTCGC
CTAAAAAGGTGGCGGAGAGCGATCACCTGGCCGTGATACTGGAGCTGGTGGCCGCCGGCGTCGGCGCGGCGCTGGTGCGC
GAGGAAGAGGCCTTGGCATGGGAGGCGGAAGGGCGCGTCTGGCTGGTGCCCGATTTGCGCAAGCGAGCCGAATTGCAGTT
CGTCTACCCGGCGGACCGGGCCGGCGATCCGGTGCTGGAGACCTTGTTGAGAGAGCTGCTGGCGGTCTGGGGACTGCCCT
GTCCGCAAGCTTGA

Upstream 100 bases:

>100_bases
CCGCAGGCGGTTTTGTTTTGGCCGCGCCCGACGTGTTAGCCTAGGCGACATTTGGCATAATCCGGCCGAAGATGTCTTCG
GCCGGCACGCAGGGGAGAGC

Downstream 100 bases:

>100_bases
GTCCGGGCGCGTCGGCGCCGTCGAATGGGGAGGCCGCATGGCCAGTTGGATAGTCGGAGCGATGGAAACGTATCGCGGCG
CGGTGGAGCAGGGGCAGCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Bsd operon regulatory protein [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MEIYQLRTFVTVAQQGHLTQAAELLHLSQPAVTAQIKALEEEVGMPLFERNAGGVSLTRAGQELLPQAQGVLAAARDIIN
HAKQLKGQLSGQARVGVTLMPDILKLGPWVAALVSAHPLLEVQLTHGVSVDVLNLVRKKELDAGFYLGKNPYMNVSTVPL
QTLDFRVALPPAWAGQMASGKLKDLGKLPWVGISQFSSLSKITAELWREINISPKKVAESDHLAVILELVAAGVGAALVR
EEEALAWEAEGRVWLVPDLRKRAELQFVYPADRAGDPVLETLLRELLAVWGLPCPQA

Sequences:

>Translated_297_residues
MEIYQLRTFVTVAQQGHLTQAAELLHLSQPAVTAQIKALEEEVGMPLFERNAGGVSLTRAGQELLPQAQGVLAAARDIIN
HAKQLKGQLSGQARVGVTLMPDILKLGPWVAALVSAHPLLEVQLTHGVSVDVLNLVRKKELDAGFYLGKNPYMNVSTVPL
QTLDFRVALPPAWAGQMASGKLKDLGKLPWVGISQFSSLSKITAELWREINISPKKVAESDHLAVILELVAAGVGAALVR
EEEALAWEAEGRVWLVPDLRKRAELQFVYPADRAGDPVLETLLRELLAVWGLPCPQA
>Mature_297_residues
MEIYQLRTFVTVAQQGHLTQAAELLHLSQPAVTAQIKALEEEVGMPLFERNAGGVSLTRAGQELLPQAQGVLAAARDIIN
HAKQLKGQLSGQARVGVTLMPDILKLGPWVAALVSAHPLLEVQLTHGVSVDVLNLVRKKELDAGFYLGKNPYMNVSTVPL
QTLDFRVALPPAWAGQMASGKLKDLGKLPWVGISQFSSLSKITAELWREINISPKKVAESDHLAVILELVAAGVGAALVR
EEEALAWEAEGRVWLVPDLRKRAELQFVYPADRAGDPVLETLLRELLAVWGLPCPQA

Specific function: Could be a positive regulator of bsdBCD expression in response to salicylic acid [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787601, Length=241, Percent_Identity=29.045643153527, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI157672245, Length=233, Percent_Identity=27.8969957081545, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI2367136, Length=267, Percent_Identity=24.7191011235955, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788887, Length=251, Percent_Identity=28.6852589641434, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790208, Length=249, Percent_Identity=28.9156626506024, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI145693105, Length=164, Percent_Identity=25, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788297, Length=254, Percent_Identity=27.1653543307087, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787879, Length=98, Percent_Identity=36.734693877551, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790399, Length=148, Percent_Identity=31.7567567567568, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788748, Length=244, Percent_Identity=25.8196721311475, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789204, Length=262, Percent_Identity=24.0458015267176, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI48994962, Length=87, Percent_Identity=37.9310344827586, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788481, Length=193, Percent_Identity=26.4248704663212, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI87081904, Length=82, Percent_Identity=46.3414634146341, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32266; Mature: 32266

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIYQLRTFVTVAQQGHLTQAAELLHLSQPAVTAQIKALEEEVGMPLFERNAGGVSLTRA
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECCCCCEEHHHC
GQELLPQAQGVLAAARDIINHAKQLKGQLSGQARVGVTLMPDILKLGPWVAALVSAHPLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECEEECHHHHHHHHHHHHHHCCCCEE
EVQLTHGVSVDVLNLVRKKELDAGFYLGKNPYMNVSTVPLQTLDFRVALPPAWAGQMASG
EEEECCCCCHHHHHHHHHHHCCCCEEECCCCCCCEEECCEEEEEEEEECCCCCCCCCCCC
KLKDLGKLPWVGISQFSSLSKITAELWREINISPKKVAESDHLAVILELVAAGVGAALVR
CHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHCHHHHHHH
EEEALAWEAEGRVWLVPDLRKRAELQFVYPADRAGDPVLETLLRELLAVWGLPCPQA
HHHHHEEECCCCEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEIYQLRTFVTVAQQGHLTQAAELLHLSQPAVTAQIKALEEEVGMPLFERNAGGVSLTRA
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECCCCCEEHHHC
GQELLPQAQGVLAAARDIINHAKQLKGQLSGQARVGVTLMPDILKLGPWVAALVSAHPLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECEEECHHHHHHHHHHHHHHCCCCEE
EVQLTHGVSVDVLNLVRKKELDAGFYLGKNPYMNVSTVPLQTLDFRVALPPAWAGQMASG
EEEECCCCCHHHHHHHHHHHCCCCEEECCCCCCCEEECCEEEEEEEEECCCCCCCCCCCC
KLKDLGKLPWVGISQFSSLSKITAELWREINISPKKVAESDHLAVILELVAAGVGAALVR
CHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHCHHHHHHH
EEEALAWEAEGRVWLVPDLRKRAELQFVYPADRAGDPVLETLLRELLAVWGLPCPQA
HHHHHEEECCCCEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]