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The map label for this gene is paaF [H]

Identifier: 34498517

GI number: 34498517

Start: 3341427

End: 3342236

Strand: Reverse

Name: paaF [H]

Synonym: CV_3062

Alternate gene names: 34498517

Gene position: 3342236-3341427 (Counterclockwise)

Preceding gene: 34498519

Following gene: 34498516

Centisome position: 70.35

GC content: 65.93

Gene sequence:

>810_bases
ATGGGGATACATGAGGAACACAATATGGCCAGGTATCTGACACTTGAACGGCTGGGAAAGTCGGCCATCATCACGCTTTC
CAACCTGCCTGCCAATCTGCTGACGACCGATGGCCTGAACGAGCTGACGGCGACGCTGCAGGCGTTGAATGCGGATGACG
ACGTGCGTTCGGTGGTGATTACCGGCGCCGGCGACGCGTTTTTTTCCGCCGGAGCGGATTTGAAGCAGTTCGCCGCAGGC
GACAAGGCGGCGGCGGACACCTTGCTGCAGGCCTTCGCCGACACGCTGCAGGCGATACGCGCCTATCGCGGCGTGACTGT
GGCAGCGGTGAACGGTTTCGCGCTGGGCGGCGGTCTGGAGTGCGCGCTGGTGTGCGACTACATCATCGCCGAGCGGGGCG
CCAAGCTGGGCCTGCCTGAAGCCAAGGTCGGACTGATTCCGGCCGCCGGGGGCACCAAGACGCTGGCCGACAAGGTGGGC
GTGTCCTGGGCCAAGCGCATCATTCTGGGCGGCGAGGTGGTGTCGGCCGAGCAGGCGCTGAAGATAGGCCTGATCGAAGA
GGTGGTGGATCAGGGCTTCGCCAAGATCGTCGCGGTCAGCCTGGCCAACAAGGTCGCAGGACAGAGCCCGGCCGCGGTGG
CGGTGGCGCGCAAGCTGATCGAGGATAGCCCCAACCTGACCCTGGACGAGCATCTGAAGCGGGAACGCGCGGCGACGCTG
GGCCTGGTCGGCGGCTCGGAGCAGCTCGACGGCGTGGCGGCTTTCCTGGCCAAGCGCGCGCCAAGCTGGGCGCAGGACGA
CGACGATTGA

Upstream 100 bases:

>100_bases
CCTGCGTGGCGGACGCGGCCCATGCCTGGAGCCGGGCCGGACTCGGGAGCGGTTCGTCGATGAAGACGAGTCGATCCGGC
CGAGGCTGCAATGAAAAGGA

Downstream 100 bases:

>100_bases
GCCGTCGCCGCCTTTGCGAGCCGATATGACAAAAGCCGCCTGCGCGCAATATTCGCCGCAGGCGGTTTTGTTTTGGCCGC
GCCCGACGTGTTAGCCTAGG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MGIHEEHNMARYLTLERLGKSAIITLSNLPANLLTTDGLNELTATLQALNADDDVRSVVITGAGDAFFSAGADLKQFAAG
DKAAADTLLQAFADTLQAIRAYRGVTVAAVNGFALGGGLECALVCDYIIAERGAKLGLPEAKVGLIPAAGGTKTLADKVG
VSWAKRIILGGEVVSAEQALKIGLIEEVVDQGFAKIVAVSLANKVAGQSPAAVAVARKLIEDSPNLTLDEHLKRERAATL
GLVGGSEQLDGVAAFLAKRAPSWAQDDDD

Sequences:

>Translated_269_residues
MGIHEEHNMARYLTLERLGKSAIITLSNLPANLLTTDGLNELTATLQALNADDDVRSVVITGAGDAFFSAGADLKQFAAG
DKAAADTLLQAFADTLQAIRAYRGVTVAAVNGFALGGGLECALVCDYIIAERGAKLGLPEAKVGLIPAAGGTKTLADKVG
VSWAKRIILGGEVVSAEQALKIGLIEEVVDQGFAKIVAVSLANKVAGQSPAAVAVARKLIEDSPNLTLDEHLKRERAATL
GLVGGSEQLDGVAAFLAKRAPSWAQDDDD
>Mature_268_residues
GIHEEHNMARYLTLERLGKSAIITLSNLPANLLTTDGLNELTATLQALNADDDVRSVVITGAGDAFFSAGADLKQFAAGD
KAAADTLLQAFADTLQAIRAYRGVTVAAVNGFALGGGLECALVCDYIIAERGAKLGLPEAKVGLIPAAGGTKTLADKVGV
SWAKRIILGGEVVSAEQALKIGLIEEVVDQGFAKIVAVSLANKVAGQSPAAVAVARKLIEDSPNLTLDEHLKRERAATLG
LVGGSEQLDGVAAFLAKRAPSWAQDDDD

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=32.8125, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI68989263, Length=218, Percent_Identity=35.3211009174312, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI4502327, Length=236, Percent_Identity=33.4745762711864, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI20127408, Length=170, Percent_Identity=35.2941176470588, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI62530384, Length=181, Percent_Identity=29.2817679558011, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI261878539, Length=114, Percent_Identity=38.5964912280702, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI213417737, Length=237, Percent_Identity=25.7383966244726, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI157694516, Length=237, Percent_Identity=25.7383966244726, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI31542718, Length=235, Percent_Identity=28.0851063829787, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI157694520, Length=202, Percent_Identity=26.7326732673267, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI295842266, Length=181, Percent_Identity=28.1767955801105, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=37.6470588235294, Blast_Score=147, Evalue=5e-37,
Organism=Escherichia coli, GI221142681, Length=259, Percent_Identity=32.046332046332, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1788682, Length=203, Percent_Identity=35.9605911330049, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1790281, Length=161, Percent_Identity=36.0248447204969, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=33.0677290836653, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=22.3076923076923, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788597, Length=232, Percent_Identity=27.1551724137931, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=36.1867704280156, Blast_Score=139, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI25145438, Length=258, Percent_Identity=34.1085271317829, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17540714, Length=215, Percent_Identity=35.3488372093023, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17558304, Length=193, Percent_Identity=34.1968911917098, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17540306, Length=246, Percent_Identity=26.8292682926829, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=180, Percent_Identity=35.5555555555556, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17508953, Length=180, Percent_Identity=35.5555555555556, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17508951, Length=180, Percent_Identity=35.5555555555556, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=223, Percent_Identity=29.1479820627803, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=257, Percent_Identity=23.3463035019455, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129971, Length=266, Percent_Identity=33.8345864661654, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24653477, Length=266, Percent_Identity=33.8345864661654, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24653139, Length=242, Percent_Identity=30.9917355371901, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24650670, Length=195, Percent_Identity=32.3076923076923, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24583165, Length=169, Percent_Identity=26.0355029585799, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21357171, Length=183, Percent_Identity=30.0546448087432, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922422, Length=265, Percent_Identity=23.3962264150943, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24654903, Length=181, Percent_Identity=29.2817679558011, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921000, Length=173, Percent_Identity=34.6820809248555, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24583077, Length=173, Percent_Identity=34.6820809248555, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24583079, Length=173, Percent_Identity=34.6820809248555, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27843; Mature: 27712

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIHEEHNMARYLTLERLGKSAIITLSNLPANLLTTDGLNELTATLQALNADDDVRSVVI
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCHHHHHHHHHHHCCCCCCEEEEE
TGAGDAFFSAGADLKQFAAGDKAAADTLLQAFADTLQAIRAYRGVTVAAVNGFALGGGLE
ECCCHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCH
CALVCDYIIAERGAKLGLPEAKVGLIPAAGGTKTLADKVGVSWAKRIILGGEVVSAEQAL
HHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHH
KIGLIEEVVDQGFAKIVAVSLANKVAGQSPAAVAVARKLIEDSPNLTLDEHLKRERAATL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHE
GLVGGSEQLDGVAAFLAKRAPSWAQDDDD
EECCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GIHEEHNMARYLTLERLGKSAIITLSNLPANLLTTDGLNELTATLQALNADDDVRSVVI
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCHHHHHHHHHHHCCCCCCEEEEE
TGAGDAFFSAGADLKQFAAGDKAAADTLLQAFADTLQAIRAYRGVTVAAVNGFALGGGLE
ECCCHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCH
CALVCDYIIAERGAKLGLPEAKVGLIPAAGGTKTLADKVGVSWAKRIILGGEVVSAEQAL
HHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHH
KIGLIEEVVDQGFAKIVAVSLANKVAGQSPAAVAVARKLIEDSPNLTLDEHLKRERAATL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHE
GLVGGSEQLDGVAAFLAKRAPSWAQDDDD
EECCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]