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The map label for this gene is pyrF

Identifier: 34498497

GI number: 34498497

Start: 3320236

End: 3320979

Strand: Reverse

Name: pyrF

Synonym: CV_3042

Alternate gene names: 34498497

Gene position: 3320979-3320236 (Counterclockwise)

Preceding gene: 34498498

Following gene: 34498496

Centisome position: 69.9

GC content: 69.49

Gene sequence:

>744_bases
ATGAATCCTCTGATCGCACAGGGCGAAAGCCGGACGACGTCCCCGGTGGTGGTGGCGCTGGATTTCGCCGACGCGGACGG
CGCGCTCGCCTTCGCCAGCCGCCTGGATCCGGCGGAATGCCGGCTGAAGGTCGGCAAGGAGCTGTTCACCTCCAGCGGCC
GCCACTTGGTCGAATCGCTGGCCGCGCGCGGCTTCCAGGTTTTCCTGGACATGAAATTCCACGACATCCCCAATACCGTG
GCCCAGGCCTGCAAGGCCGCGGCCGAGTCCGGCGTGTGGATGGTCAACGTCCACGCCTCCGGCGGCCGCCGGATGATGGA
GGCCGCGCGCGAGGCGCTGGCCGGCTACAGCCAGCGCCCGCTGCTGATCGCGGTGACGGTGTTGACCAGCATGGAGGCGT
CGGACCTGGCCGAGGTCGGCATCGCCGCCACGCCGCAGGAGCACGTGCTGCGCCTGGCCACGCTGACCCGCGATTGCGGC
CTGGACGGCGTGGTGTGCTCGGCGCAGGAGGCCGCGATGCTGAAGCTGGCGCTGGGCCGCGATTTCAAGCTGGTGACGCC
GGGCATCCGTCTCGCCGACAGCGCCGGCGACGACCAGCGCCGCGTGATGACGCCGACCGCCGCCTTGGCCGCCGGCTCGG
ACTATCTGGTGATCGGCCGGCCGATCACCCGCGCCGCGGACCCGTTGGCCGCGCTGCGCGCCATCAATCAAGACATTTCC
GTATTCCTGGGTAAACAATCATGA

Upstream 100 bases:

>100_bases
GAATGGGAAAGCTTCACCGCCAACCGTTCGGAAAACTGAATCACTCGGCGCGCGCTGTCTTGATGGCGCGCGCCGCGCAA
TATGGAATGATGGAGCATCG

Downstream 100 bases:

>100_bases
AAGTAACGGTAATTGGCTCGGGCTATGTCGGCCTGGTGACCGGCACCTGCCTGGCCGAGACCGGCTACCACGTCTGCTGC
CTGGACGTGGATCCGCGCAA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MNPLIAQGESRTTSPVVVALDFADADGALAFASRLDPAECRLKVGKELFTSSGRHLVESLAARGFQVFLDMKFHDIPNTV
AQACKAAAESGVWMVNVHASGGRRMMEAAREALAGYSQRPLLIAVTVLTSMEASDLAEVGIAATPQEHVLRLATLTRDCG
LDGVVCSAQEAAMLKLALGRDFKLVTPGIRLADSAGDDQRRVMTPTAALAAGSDYLVIGRPITRAADPLAALRAINQDIS
VFLGKQS

Sequences:

>Translated_247_residues
MNPLIAQGESRTTSPVVVALDFADADGALAFASRLDPAECRLKVGKELFTSSGRHLVESLAARGFQVFLDMKFHDIPNTV
AQACKAAAESGVWMVNVHASGGRRMMEAAREALAGYSQRPLLIAVTVLTSMEASDLAEVGIAATPQEHVLRLATLTRDCG
LDGVVCSAQEAAMLKLALGRDFKLVTPGIRLADSAGDDQRRVMTPTAALAAGSDYLVIGRPITRAADPLAALRAINQDIS
VFLGKQS
>Mature_247_residues
MNPLIAQGESRTTSPVVVALDFADADGALAFASRLDPAECRLKVGKELFTSSGRHLVESLAARGFQVFLDMKFHDIPNTV
AQACKAAAESGVWMVNVHASGGRRMMEAAREALAGYSQRPLLIAVTVLTSMEASDLAEVGIAATPQEHVLRLATLTRDCG
LDGVVCSAQEAAMLKLALGRDFKLVTPGIRLADSAGDDQRRVMTPTAALAAGSDYLVIGRPITRAADPLAALRAINQDIS
VFLGKQS

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=236, Percent_Identity=62.2881355932203, Blast_Score=285, Evalue=2e-78,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_CHRVO (Q7NTL2)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902712.1
- ProteinModelPortal:   Q7NTL2
- SMR:   Q7NTL2
- GeneID:   2547500
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3042
- NMPDR:   fig|243365.1.peg.3042
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   Q7NTL2
- ProtClustDB:   PRK00230
- BioCyc:   CVIO243365:CV_3042-MONOMER
- BRENDA:   4.1.1.23
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 26088; Mature: 26088

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 72-72 BINDING 21-21 BINDING 43-43 BINDING 129-129 BINDING 190-190 BINDING 199-199 BINDING 219-219 BINDING 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLIAQGESRTTSPVVVALDFADADGALAFASRLDPAECRLKVGKELFTSSGRHLVESL
CCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHH
AARGFQVFLDMKFHDIPNTVAQACKAAAESGVWMVNVHASGGRRMMEAAREALAGYSQRP
HHCCEEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHCCCCCCC
LLIAVTVLTSMEASDLAEVGIAATPQEHVLRLATLTRDCGLDGVVCSAQEAAMLKLALGR
EEEEEEEHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCC
DFKLVTPGIRLADSAGDDQRRVMTPTAALAAGSDYLVIGRPITRAADPLAALRAINQDIS
CEEEECCCCEEECCCCCCCCEEECCHHHHHCCCCEEEECCCHHHHCCHHHHHHHHCCCCE
VFLGKQS
EEEECCC
>Mature Secondary Structure
MNPLIAQGESRTTSPVVVALDFADADGALAFASRLDPAECRLKVGKELFTSSGRHLVESL
CCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHH
AARGFQVFLDMKFHDIPNTVAQACKAAAESGVWMVNVHASGGRRMMEAAREALAGYSQRP
HHCCEEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHCCCCCCC
LLIAVTVLTSMEASDLAEVGIAATPQEHVLRLATLTRDCGLDGVVCSAQEAAMLKLALGR
EEEEEEEHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCC
DFKLVTPGIRLADSAGDDQRRVMTPTAALAAGSDYLVIGRPITRAADPLAALRAINQDIS
CEEEECCCCEEECCCCCCCCEEECCHHHHHCCCCEEEECCCHHHHCCHHHHHHHHCCCCE
VFLGKQS
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782