Click here to switch to the map view.

The map label for this gene is yafJ [C]

Identifier: 34498399

GI number: 34498399

Start: 3222827

End: 3223609

Strand: Direct

Name: yafJ [C]

Synonym: CV_2944

Alternate gene names: 34498399

Gene position: 3222827-3223609 (Clockwise)

Preceding gene: 34498394

Following gene: 34498400

Centisome position: 67.83

GC content: 65.39

Gene sequence:

>783_bases
ATGTGCCAGCTGCTGGGCATGAATTGCAACACCCCTACCGACATTCTGTTTTCCTTCGAAGGCTTCCACCGCCGCGGAGG
CCTCACCGACCACCATGCCGACGGCTGGGGCATCGCCTTCTTCGAAGACAAGGGTTGTCGCGTCTTCCTCGACGACAAGC
CGTCGGTGGAGTCGCCGGTCGCCGACCTGGTGCGGCGCTACCCGATCAAGTCCGAGAACGTCATCGCCCACATCCGCAAG
GCGACGCAGGGCGAGGTCAATCTGGCCAACACCCACCCGTTCATGCGCGAGATGTGGGGCCAGTACTGGATCTTCGCCCA
CAACGGCAATCTGGAGAGCTTCCATCCCGAAGCCGGCGAACACTACCGCGCGGTCGGCACCACCGATTCCGAGCGCGCGT
TCTGCCACCTGCTGGAAAAGCTGCGCGCCAAATGGGCGGAGCCGCCGGAAGCGGAAGCGCTGTTCGAGGAGGTGGCCAGG
CTGGCCGCCGAGATCAGCGCCCGCGGCGTGTTCAACTTCATGTTGAGCAACGGCGAGGCGCTGTTCGTCCATTGCTCGAC
CCATCTGCACTACATCATCCGCCGCGCGCCGTTCAACACCGCCCACTTGGTGGACGACGACGTCAGCGTGGACTTCTCCA
CCGTCACCACGCCGCGCGACCAGGTGGCGATGATCGCCACCCAGCCGCTGACCGACAACGAGGCCTGGACCGCGCTCTCC
CCTGGCGAACTGATCCTGTTCCGCGACGGCGCGCCGCTGCTGTCGCGCAAGCCGGCAGCCTGA

Upstream 100 bases:

>100_bases
CGATTGCCCGCCGCCGCCAGATCGCCTAAGCTCAGCTACAAGACCATCCCGGCAGCAGGCCGGAGCCGCGCAACCCGTTT
CGAGAAGTGGAATCAAGCCG

Downstream 100 bases:

>100_bases
CACCGGAACGGCGATAGGCGTTATGCTGACGGGAACCGCCTTCAGCACGGATACGCCATGAGCCGTTTCGACCTCAGCCA
GCCGCCGTTCGACAACCTCA

Product: amidotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MCQLLGMNCNTPTDILFSFEGFHRRGGLTDHHADGWGIAFFEDKGCRVFLDDKPSVESPVADLVRRYPIKSENVIAHIRK
ATQGEVNLANTHPFMREMWGQYWIFAHNGNLESFHPEAGEHYRAVGTTDSERAFCHLLEKLRAKWAEPPEAEALFEEVAR
LAAEISARGVFNFMLSNGEALFVHCSTHLHYIIRRAPFNTAHLVDDDVSVDFSTVTTPRDQVAMIATQPLTDNEAWTALS
PGELILFRDGAPLLSRKPAA

Sequences:

>Translated_260_residues
MCQLLGMNCNTPTDILFSFEGFHRRGGLTDHHADGWGIAFFEDKGCRVFLDDKPSVESPVADLVRRYPIKSENVIAHIRK
ATQGEVNLANTHPFMREMWGQYWIFAHNGNLESFHPEAGEHYRAVGTTDSERAFCHLLEKLRAKWAEPPEAEALFEEVAR
LAAEISARGVFNFMLSNGEALFVHCSTHLHYIIRRAPFNTAHLVDDDVSVDFSTVTTPRDQVAMIATQPLTDNEAWTALS
PGELILFRDGAPLLSRKPAA
>Mature_260_residues
MCQLLGMNCNTPTDILFSFEGFHRRGGLTDHHADGWGIAFFEDKGCRVFLDDKPSVESPVADLVRRYPIKSENVIAHIRK
ATQGEVNLANTHPFMREMWGQYWIFAHNGNLESFHPEAGEHYRAVGTTDSERAFCHLLEKLRAKWAEPPEAEALFEEVAR
LAAEISARGVFNFMLSNGEALFVHCSTHLHYIIRRAPFNTAHLVDDDVSVDFSTVTTPRDQVAMIATQPLTDNEAWTALS
PGELILFRDGAPLLSRKPAA

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=248, Percent_Identity=55.241935483871, Blast_Score=291, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 29192; Mature: 29192

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQLLGMNCNTPTDILFSFEGFHRRGGLTDHHADGWGIAFFEDKGCRVFLDDKPSVESPV
CCEEEECCCCCCHHEEEEECCHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCHH
ADLVRRYPIKSENVIAHIRKATQGEVNLANTHPFMREMWGQYWIFAHNGNLESFHPEAGE
HHHHHHCCCCCCHHHHHHHHHCCCCEEECCCCHHHHHHCCCEEEEEECCCCCCCCCCCCC
HYRAVGTTDSERAFCHLLEKLRAKWAEPPEAEALFEEVARLAAEISARGVFNFMLSNGEA
CEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCE
LFVHCSTHLHYIIRRAPFNTAHLVDDDVSVDFSTVTTPRDQVAMIATQPLTDNEAWTALS
EEEEECHHHEEHHHHCCCCCCEEECCCCCEEEEEECCCCHHEEEEEECCCCCCCCEEEEC
PGELILFRDGAPLLSRKPAA
CCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MCQLLGMNCNTPTDILFSFEGFHRRGGLTDHHADGWGIAFFEDKGCRVFLDDKPSVESPV
CCEEEECCCCCCHHEEEEECCHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCHH
ADLVRRYPIKSENVIAHIRKATQGEVNLANTHPFMREMWGQYWIFAHNGNLESFHPEAGE
HHHHHHCCCCCCHHHHHHHHHCCCCEEECCCCHHHHHHCCCEEEEEECCCCCCCCCCCCC
HYRAVGTTDSERAFCHLLEKLRAKWAEPPEAEALFEEVARLAAEISARGVFNFMLSNGEA
CEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCE
LFVHCSTHLHYIIRRAPFNTAHLVDDDVSVDFSTVTTPRDQVAMIATQPLTDNEAWTALS
EEEEECHHHEEHHHHCCCCCCEEECCCCCEEEEEECCCCHHEEEEEECCCCCCCCEEEEC
PGELILFRDGAPLLSRKPAA
CCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]