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The map label for this gene is flgJ [H]

Identifier: 34498335

GI number: 34498335

Start: 3157005

End: 3157934

Strand: Reverse

Name: flgJ [H]

Synonym: CV_2880

Alternate gene names: 34498335

Gene position: 3157934-3157005 (Counterclockwise)

Preceding gene: 34498336

Following gene: 34498334

Centisome position: 66.47

GC content: 65.16

Gene sequence:

>930_bases
CTGTTACCCAACGACATGCTGAGCCGCCAGCTGGCCGCCGATCCGACCGGCTTGCAAAAGCTCAAGGCGAAGGCCAGCGG
CGATCCCAAGGCCGCCGCCAAGGAAGCGGCTTCGCAGTTCGAAGCGCTGTTGATGAACACCATGCTGAAGACCATGCGCG
CGACCAAGTTCGACGAGAGCGAGGCATCCAACAGCATGGACACTTTCCAGGGCCTGTCCGACCAGCAGATGGTCCAGGCC
TTGTGCTCGCGCGGCGGCATCGGCCTGGGCGACATGATCTACCGGCAGATCGCCAAGCAGTCGGGCTTCGAGCCTGATGC
CGGGAAGGACGGCGCGGCACTGTTGTCCCACGGCCCGGCCCTGGCGGCCCGCCCGATCGGCGAGCGCGCGGCCCAGGCAT
ATCGCAATGCTTCCGGCGTTTCTGAGGCGGGCGGCAGCTCTCCGTCCCAGACCGTGGGAGGCGGCAATAAATCGTTCGTC
GGCGGCATGCTGTCGCATGCGCGGGACGCCGCCCAGCAGCTGGGCGTCGCGCCCGAGTGCGTGGTCGCCCATGCCGCGCT
GGAAAGCGGCTGGGGCAAGCGGACCATACGCAACGCCGACGGAACCGACAGCCATAACCTGTTCGGCATCAAGGCCGGCG
GCGACTGGCAGGGCAAGACGACCACGATCATGACCACCGAGTATGTGGGCGGCGTGGCGCAAAAGCGGCAAGAGACCTTC
CGTTCCTATGGTTCATACGCAGAGGCCTTTACCGATTACGCGAAAGTGCTCAAATCCTCCTCGCGTTACAAGAACGTTCT
GAATCAAGGGCAAAACGTGTACGGCTTCGCCCAGGGCCTGCAAAGCGGCGGCTACGCCACCGACCCGCGCTACGCGCGCA
AACTGGTCGACGTGGCGGCGAGCCTGGCGCAACAAGTGGCCAGGACTTGA

Upstream 100 bases:

>100_bases
GCAGGCTCCTTGAAGGCCGATCTGCAGATCATCTGACCTGGCATGATTATTGCTGGGTTTGGGGCCGAGGAGCGAAGAAA
TGACCGCGAAATTCCAGGGC

Downstream 100 bases:

>100_bases
TCAAGCTTTGGCGAAAACGGTCGATAAAGGCTTAGAGAGGTAATCATGGCTATCATCGGAGCGGACGTCTTTGGCATCGG
CCTGAGCGGACTGAATGTCG

Product: flagellar protein flgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MLPNDMLSRQLAADPTGLQKLKAKASGDPKAAAKEAASQFEALLMNTMLKTMRATKFDESEASNSMDTFQGLSDQQMVQA
LCSRGGIGLGDMIYRQIAKQSGFEPDAGKDGAALLSHGPALAARPIGERAAQAYRNASGVSEAGGSSPSQTVGGGNKSFV
GGMLSHARDAAQQLGVAPECVVAHAALESGWGKRTIRNADGTDSHNLFGIKAGGDWQGKTTTIMTTEYVGGVAQKRQETF
RSYGSYAEAFTDYAKVLKSSSRYKNVLNQGQNVYGFAQGLQSGGYATDPRYARKLVDVAASLAQQVART

Sequences:

>Translated_309_residues
MLPNDMLSRQLAADPTGLQKLKAKASGDPKAAAKEAASQFEALLMNTMLKTMRATKFDESEASNSMDTFQGLSDQQMVQA
LCSRGGIGLGDMIYRQIAKQSGFEPDAGKDGAALLSHGPALAARPIGERAAQAYRNASGVSEAGGSSPSQTVGGGNKSFV
GGMLSHARDAAQQLGVAPECVVAHAALESGWGKRTIRNADGTDSHNLFGIKAGGDWQGKTTTIMTTEYVGGVAQKRQETF
RSYGSYAEAFTDYAKVLKSSSRYKNVLNQGQNVYGFAQGLQSGGYATDPRYARKLVDVAASLAQQVART
>Mature_309_residues
MLPNDMLSRQLAADPTGLQKLKAKASGDPKAAAKEAASQFEALLMNTMLKTMRATKFDESEASNSMDTFQGLSDQQMVQA
LCSRGGIGLGDMIYRQIAKQSGFEPDAGKDGAALLSHGPALAARPIGERAAQAYRNASGVSEAGGSSPSQTVGGGNKSFV
GGMLSHARDAAQQLGVAPECVVAHAALESGWGKRTIRNADGTDSHNLFGIKAGGDWQGKTTTIMTTEYVGGVAQKRQETF
RSYGSYAEAFTDYAKVLKSSSRYKNVLNQGQNVYGFAQGLQSGGYATDPRYARKLVDVAASLAQQVART

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=316, Percent_Identity=35.7594936708861, Blast_Score=174, Evalue=6e-45,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 32562; Mature: 32562

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPNDMLSRQLAADPTGLQKLKAKASGDPKAAAKEAASQFEALLMNTMLKTMRATKFDES
CCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EASNSMDTFQGLSDQQMVQALCSRGGIGLGDMIYRQIAKQSGFEPDAGKDGAALLSHGPA
HHCCCHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCH
LAARPIGERAAQAYRNASGVSEAGGSSPSQTVGGGNKSFVGGMLSHARDAAQQLGVAPEC
HHHCCHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
VVAHAALESGWGKRTIRNADGTDSHNLFGIKAGGDWQGKTTTIMTTEYVGGVAQKRQETF
HHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHH
RSYGSYAEAFTDYAKVLKSSSRYKNVLNQGQNVYGFAQGLQSGGYATDPRYARKLVDVAA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
SLAQQVART
HHHHHHHCC
>Mature Secondary Structure
MLPNDMLSRQLAADPTGLQKLKAKASGDPKAAAKEAASQFEALLMNTMLKTMRATKFDES
CCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EASNSMDTFQGLSDQQMVQALCSRGGIGLGDMIYRQIAKQSGFEPDAGKDGAALLSHGPA
HHCCCHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCH
LAARPIGERAAQAYRNASGVSEAGGSSPSQTVGGGNKSFVGGMLSHARDAAQQLGVAPEC
HHHCCHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
VVAHAALESGWGKRTIRNADGTDSHNLFGIKAGGDWQGKTTTIMTTEYVGGVAQKRQETF
HHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHH
RSYGSYAEAFTDYAKVLKSSSRYKNVLNQGQNVYGFAQGLQSGGYATDPRYARKLVDVAA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
SLAQQVART
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]