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The map label for this gene is phaA

Identifier: 34498245

GI number: 34498245

Start: 3024658

End: 3025836

Strand: Direct

Name: phaA

Synonym: CV_2790

Alternate gene names: 34498245

Gene position: 3024658-3025836 (Clockwise)

Preceding gene: 34498244

Following gene: 34498247

Centisome position: 63.66

GC content: 69.55

Gene sequence:

>1179_bases
ATGGAAGTAGCAATCGTAGCCGCGCAGCGCACCGCCATCGGCAGCTTCGGCGGCGGCTTGGCCAAGATTCCGGCGCCGGA
GCTGGGCGCCACTGTGATCAAGGCGTTGCTGGAGAAGACCGGCGTCAAACCGGAAGACGTCAGCGAAGTGATCCTGGGCC
AGGTGCTGACCGCCGGCAGCGGCCAGAACCCGGCCCGCCAGGCGCTGATCAAGGCCGGCCTGCCGGTCACCACTCCGGCC
ACCACGCTGAACGTGGTGTGCGGCTCCGGCCTGCGCGCCGTGCATCTGGCGGCCCAGGCCATCCTGGCCGGCGACGCCGA
CATCGTCATCGCCGGCGGCCAGGAAAGCATGTCGCTGTCCCCGCACATCCTGCCGGGCTCGCGCGACGGTTTCCGCATGG
GCAACGCCCAACTGGTGGACACCATGGTCAACGACGGCCTGACCGACGCCTACAACGCCTACCACATGGGCATCACCGCT
GAGAACGTGGCGGCCAAATACGGCATCGGCCGCGAAGAGCAGGACGCCTTCTCGCTGCAGTCGCAGCAGCGCGCCGCCGC
CGCCCAGAAGGCCGGCAAATTCCGGGACGAGATCGTTCCGGTGCTGGTGCCGCAGCGCAAGGGCGATCCGCTGGCCTTCG
ACGCCGACGAGTTCATCAAGCACGACGCCAGCGCCGACGGCCTGGCCAAGCTGCGCCCGGCCTTCAAGAAGGACGGCACG
GTCACCGCCGGCAACGCCTCCGGCATCAACGACGGCGCCGCCGCTGTGCTGCTGATGTCCACGCAAAAGGCCGATCAGCT
GGGCCTGAAGCCGCTGGCCATCATCAAGGGCTACGCGCTGACCGGCTGCGAGCCGGAAATCATGGGCATCGGCCCGGTAT
CCGCCACCCGCAAGGCGCTGTCCAAGGCCGGCTGGACGGTGGAAGACCTGGACCTGGTGGAGGCCAACGAGGCCTTCGCC
GCTCAGGCGCTGGGCGTGGCCAAGGAGCTGGGCTGGGGCTCGGACAAGGTCAACGTCAACGGCGGCGCCATCGCGCTGGG
CCACCCGATCGGCGCCTCCGGCTGCCGCGTGCTGGTGACCCTGCTGCACGAAATGCAGCGCCGCGGCGCCAAGAAGGGCC
TGGCCACGCTGTGCATCGGCGGCGGCATGGGCGTGGCGCTGGCGGTCGAGCGTCCGTAA

Upstream 100 bases:

>100_bases
ATGCCGTCCGTCGCCGCCAGCCTGCAATGACAACCCTATTCGAACGCCCCGGAGCGGCATGATCCGCTCCCGACGAGGGC
CGCAACAAAAGGAGAAAGAC

Downstream 100 bases:

>100_bases
ACGCCCGCTTCGCAAGTACGAAACCCGCCGAATCCGGCGGGTTTTGTTTTGCCCAATCGTTTAGGCCAGGCTGATGCCCA
ATATGCGCTCAAGCGCTTGG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MEVAIVAAQRTAIGSFGGGLAKIPAPELGATVIKALLEKTGVKPEDVSEVILGQVLTAGSGQNPARQALIKAGLPVTTPA
TTLNVVCGSGLRAVHLAAQAILAGDADIVIAGGQESMSLSPHILPGSRDGFRMGNAQLVDTMVNDGLTDAYNAYHMGITA
ENVAAKYGIGREEQDAFSLQSQQRAAAAQKAGKFRDEIVPVLVPQRKGDPLAFDADEFIKHDASADGLAKLRPAFKKDGT
VTAGNASGINDGAAAVLLMSTQKADQLGLKPLAIIKGYALTGCEPEIMGIGPVSATRKALSKAGWTVEDLDLVEANEAFA
AQALGVAKELGWGSDKVNVNGGAIALGHPIGASGCRVLVTLLHEMQRRGAKKGLATLCIGGGMGVALAVERP

Sequences:

>Translated_392_residues
MEVAIVAAQRTAIGSFGGGLAKIPAPELGATVIKALLEKTGVKPEDVSEVILGQVLTAGSGQNPARQALIKAGLPVTTPA
TTLNVVCGSGLRAVHLAAQAILAGDADIVIAGGQESMSLSPHILPGSRDGFRMGNAQLVDTMVNDGLTDAYNAYHMGITA
ENVAAKYGIGREEQDAFSLQSQQRAAAAQKAGKFRDEIVPVLVPQRKGDPLAFDADEFIKHDASADGLAKLRPAFKKDGT
VTAGNASGINDGAAAVLLMSTQKADQLGLKPLAIIKGYALTGCEPEIMGIGPVSATRKALSKAGWTVEDLDLVEANEAFA
AQALGVAKELGWGSDKVNVNGGAIALGHPIGASGCRVLVTLLHEMQRRGAKKGLATLCIGGGMGVALAVERP
>Mature_392_residues
MEVAIVAAQRTAIGSFGGGLAKIPAPELGATVIKALLEKTGVKPEDVSEVILGQVLTAGSGQNPARQALIKAGLPVTTPA
TTLNVVCGSGLRAVHLAAQAILAGDADIVIAGGQESMSLSPHILPGSRDGFRMGNAQLVDTMVNDGLTDAYNAYHMGITA
ENVAAKYGIGREEQDAFSLQSQQRAAAAQKAGKFRDEIVPVLVPQRKGDPLAFDADEFIKHDASADGLAKLRPAFKKDGT
VTAGNASGINDGAAAVLLMSTQKADQLGLKPLAIIKGYALTGCEPEIMGIGPVSATRKALSKAGWTVEDLDLVEANEAFA
AQALGVAKELGWGSDKVNVNGGAIALGHPIGASGCRVLVTLLHEMQRRGAKKGLATLCIGGGMGVALAVERP

Specific function: Involved in the production of polyhydroxyalkonic acids (PHAs), composed primarily of 3-hydroxybutyric acid (3HB) and 3- hydroxyvaleric acid (3HV)

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family

Homologues:

Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=56.0102301790281, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=45.5012853470437, Blast_Score=326, Evalue=2e-89,
Organism=Homo sapiens, GI4557237, Length=393, Percent_Identity=44.529262086514, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI4501853, Length=397, Percent_Identity=44.5843828715365, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI4504327, Length=424, Percent_Identity=31.8396226415094, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI194353979, Length=397, Percent_Identity=33.5012594458438, Blast_Score=168, Evalue=7e-42,
Organism=Escherichia coli, GI87082165, Length=390, Percent_Identity=62.5641025641026, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1788554, Length=388, Percent_Identity=60.0515463917526, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1787663, Length=399, Percent_Identity=45.1127819548872, Blast_Score=314, Evalue=7e-87,
Organism=Escherichia coli, GI48994986, Length=397, Percent_Identity=42.3173803526448, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=36.5853658536585, Blast_Score=219, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=45.1282051282051, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI25147385, Length=387, Percent_Identity=42.1188630490956, Blast_Score=288, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI17535921, Length=392, Percent_Identity=40.0510204081633, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17535917, Length=389, Percent_Identity=33.4190231362468, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17551802, Length=426, Percent_Identity=31.6901408450704, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6325229, Length=403, Percent_Identity=44.6650124069479, Blast_Score=296, Evalue=3e-81,
Organism=Saccharomyces cerevisiae, GI6322031, Length=398, Percent_Identity=39.9497487437186, Blast_Score=239, Evalue=6e-64,
Organism=Drosophila melanogaster, GI24655093, Length=390, Percent_Identity=58.4615384615385, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI17648125, Length=390, Percent_Identity=45.6410256410256, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24640423, Length=391, Percent_Identity=45.0127877237852, Blast_Score=307, Evalue=7e-84,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=32.4705882352941, Blast_Score=181, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIL_CHRVO (Q9ZHI1)

Other databases:

- EMBL:   AE016825
- EMBL:   AF061446
- RefSeq:   NP_902460.1
- ProteinModelPortal:   Q9ZHI1
- SMR:   Q9ZHI1
- GeneID:   2547100
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2790
- NMPDR:   fig|243365.1.peg.2790
- HOGENOM:   HBG370930
- OMA:   MMKDVVI
- PhylomeDB:   Q9ZHI1
- ProtClustDB:   PRK05790
- BioCyc:   CVIO243365:CV_2790-MONOMER
- BRENDA:   2.3.1.9
- GO:   GO:0005737
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616
- Gene3D:   G3DSA:3.40.47.10
- PANTHER:   PTHR18919
- PIRSF:   PIRSF000429
- TIGRFAMs:   TIGR01930

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N; SSF53901 Thiolase-like

EC number: =2.3.1.9

Molecular weight: Translated: 40120; Mature: 40120

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00098 THIOLASE_1; PS00737 THIOLASE_2; PS00099 THIOLASE_3

Important sites: ACT_SITE 87-87 ACT_SITE 348-348 ACT_SITE 378-378

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVAIVAAQRTAIGSFGGGLAKIPAPELGATVIKALLEKTGVKPEDVSEVILGQVLTAGS
CCEEEEEECHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
GQNPARQALIKAGLPVTTPATTLNVVCGSGLRAVHLAAQAILAGDADIVIAGGQESMSLS
CCCHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
PHILPGSRDGFRMGNAQLVDTMVNDGLTDAYNAYHMGITAENVAAKYGIGREEQDAFSLQ
CEECCCCCCCCCCCCHHHHHHHHHCCCCHHCCCEEECCCHHHHHHHHCCCCCHHHHHHHH
SQQRAAAAQKAGKFRDEIVPVLVPQRKGDPLAFDADEFIKHDASADGLAKLRPAFKKDGT
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEECHHHHHHCCCCCCCHHHHCHHHHCCCC
VTAGNASGINDGAAAVLLMSTQKADQLGLKPLAIIKGYALTGCEPEIMGIGPVSATRKAL
EEECCCCCCCCCCEEEEEEECCCHHHHCCCHHHHHCCCEECCCCCCEEECCCHHHHHHHH
SKAGWTVEDLDLVEANEAFAAQALGVAKELGWGSDKVNVNGGAIALGHPIGASGCRVLVT
HHCCCCEECCHHEECCHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCCCCHHHHHHHH
LLHEMQRRGAKKGLATLCIGGGMGVALAVERP
HHHHHHHHHHHCCCEEEEECCCCEEEEEEECC
>Mature Secondary Structure
MEVAIVAAQRTAIGSFGGGLAKIPAPELGATVIKALLEKTGVKPEDVSEVILGQVLTAGS
CCEEEEEECHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
GQNPARQALIKAGLPVTTPATTLNVVCGSGLRAVHLAAQAILAGDADIVIAGGQESMSLS
CCCHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
PHILPGSRDGFRMGNAQLVDTMVNDGLTDAYNAYHMGITAENVAAKYGIGREEQDAFSLQ
CEECCCCCCCCCCCCHHHHHHHHHCCCCHHCCCEEECCCHHHHHHHHCCCCCHHHHHHHH
SQQRAAAAQKAGKFRDEIVPVLVPQRKGDPLAFDADEFIKHDASADGLAKLRPAFKKDGT
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEECHHHHHHCCCCCCCHHHHCHHHHCCCC
VTAGNASGINDGAAAVLLMSTQKADQLGLKPLAIIKGYALTGCEPEIMGIGPVSATRKAL
EEECCCCCCCCCCEEEEEEECCCHHHHCCCHHHHHCCCEECCCCCCEEECCCHHHHHHHH
SKAGWTVEDLDLVEANEAFAAQALGVAKELGWGSDKVNVNGGAIALGHPIGASGCRVLVT
HHCCCCEECCHHEECCHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCCCCHHHHHHHH
LLHEMQRRGAKKGLATLCIGGGMGVALAVERP
HHHHHHHHHHHCCCEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782; 10427049