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The map label for this gene is phaC

Identifier: 34498244

GI number: 34498244

Start: 3022881

End: 3024587

Strand: Direct

Name: phaC

Synonym: CV_2789

Alternate gene names: 34498244

Gene position: 3022881-3024587 (Clockwise)

Preceding gene: 34498240

Following gene: 34498245

Centisome position: 63.63

GC content: 64.26

Gene sequence:

>1707_bases
ATGCAGCAGTTCGTCAACTCGCTGTCGCAGCCTCCAGCCCCCGACGGCGCAGCCCATCCCTTCGCCGGCGCCTGGGCGCA
GCTGATGAACCAGACCAACCAGCTGTTCGCTCTCCAGTCCTCGCTTTACCAGCAGCAGCTCAATCTGTGGTCGCAATTCC
TCGGCCAGGCCGCCGGCCAGGAAGCCGCCGCCGAAGCGGGCGCCAAGCCGGCCGACCGCCGCTTCGCGTCGCCGGAATGG
AACGAGCACCCGTTCTACAACTTCCTCAAGCAGAGCTATCTGCAGACCTCCAAGTGGATGATGGAGCTGGTGGACAAGAC
CCAGCTCGACGAGGACGCCAAGGACAAGCTGGCTTTCGCCACCCGCCAGTACCTGGACGCGATGTCCCCCAGCAACTTCA
TGCTGACCAACCCCGACGTGGTCAAGCGCGCGATCGAGACCAAGGGCGAAAGCCTGGTCGAGGGCATGAAGAACATGCTG
GACGACTTCCAGAAGGGCCACATCTCGATGTCGGACGAGAGCAAGTTCGAGATCGGCAAGAACCTGGTGGTCACCCCGGG
CCAGGTGGTGTTCCGCAACGAACTGATCGAGCTGATCCAGTACACGCCGACCACCGACAAGGTCTACGAGAAGCCGCTGC
TGTTCGTGCCGCCCTGCATCAACAAGTACTACCTGATGGACCTGCAGCCGGACAACTCCATGGTGCGCCACTTCGTCGCC
CAGGGTTACCGCGTGTTCCTGATCAGCTGGCGCTCCGCGGTCGCCGAGATGAAGCACTTCACCTGGGAAACCTATATCGA
GAAAGGCGTGTTCGCCGCGGCGGAAGCGGTGCAGAAAATCACCAAGCAGCCGACGATGAACGTGCTGGGCTTCTGCGTCG
GCGGCGTCATCCTCACCACCGCGCTGTGCGTGGCTCAGGCCAAGGGGCTGAAATACTTCGACTCCGCCACCTTCATGACC
TCGCTGATCGACCACGCCGAACCGGGCGAGATCTCCTTCTTCATCGACGAGAGCGTGGTGGCCGGCCGCGAAGCCAAGAT
GGCCAGCGGCGGCATCATCAGCGGCAAGGAAATCGGCCGCACCTTCGCCAGCCTGCGCGCCAACGACCTGGTGTGGAACT
ACGTGGTCAACAACTACCTGCTGGGCAAGACCCCGGCGCCGTTCGACCTGCTGTTCTGGAACAACGACGCGGTGGATCTG
CCGCTGCCGATGCACACCTTCCTGCTGCGCCAGTTCTACATGAACAACGCGCTGGTGCGTCCGGGCGCGATCACGCTGTG
CGGCGTGCCGATCGACATCGCCAAGATCGACGTGCCGGTCTACATGTTCGCCGCGCGCGACGACCACATCGTGCTGTGGA
GCTCCGCCTTCTCCGGCCTGAAATACCTGCAGGGCGCGCCCAGCCGCCGCTTCGTGCTGGGCGCGTCCGGCCACATCGCC
GGCTCGATCAACCCGGTCACCAAGGACAAGCGCAACTACTGGGCCAACGACACGCTGCCGCTGCACGCCGAGGAATGGCT
GGAAAGCGCGGAAAGCCGCCCCGGCAGCTGGTGGAAGGACTGGGACGCCTGGCTGGCGCCGCAGTCCGGCAAGCAAGTGG
CCGCGCCCAAAAGCCTGGGCAACAAGGAATTCCCGCCGCTGCTGGCGGCGCCGGGCAGTTATGTGCTGGCCAAGGCCATG
CCGTCCGTCGCCGCCAGCCTGCAATGA

Upstream 100 bases:

>100_bases
ACAACGATTTGCACGTTTCAGGAGATTCATCTTGTCCCAGAATAGTACCGCCGCGCTGGAAGGTTTCTTCGCCAGCCTCT
CCGAGGCCAACCAGCAATGG

Downstream 100 bases:

>100_bases
CAACCCTATTCGAACGCCCCGGAGCGGCATGATCCGCTCCCGACGAGGGCCGCAACAAAAGGAGAAAGACATGGAAGTAG
CAATCGTAGCCGCGCAGCGC

Product: polyhydroxyalkanoate synthase

Products: NA

Alternate protein names: PHA polymerase; PHA synthase; Polyhydroxyalkanoic acid synthase

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MQQFVNSLSQPPAPDGAAHPFAGAWAQLMNQTNQLFALQSSLYQQQLNLWSQFLGQAAGQEAAAEAGAKPADRRFASPEW
NEHPFYNFLKQSYLQTSKWMMELVDKTQLDEDAKDKLAFATRQYLDAMSPSNFMLTNPDVVKRAIETKGESLVEGMKNML
DDFQKGHISMSDESKFEIGKNLVVTPGQVVFRNELIELIQYTPTTDKVYEKPLLFVPPCINKYYLMDLQPDNSMVRHFVA
QGYRVFLISWRSAVAEMKHFTWETYIEKGVFAAAEAVQKITKQPTMNVLGFCVGGVILTTALCVAQAKGLKYFDSATFMT
SLIDHAEPGEISFFIDESVVAGREAKMASGGIISGKEIGRTFASLRANDLVWNYVVNNYLLGKTPAPFDLLFWNNDAVDL
PLPMHTFLLRQFYMNNALVRPGAITLCGVPIDIAKIDVPVYMFAARDDHIVLWSSAFSGLKYLQGAPSRRFVLGASGHIA
GSINPVTKDKRNYWANDTLPLHAEEWLESAESRPGSWWKDWDAWLAPQSGKQVAAPKSLGNKEFPPLLAAPGSYVLAKAM
PSVAASLQ

Sequences:

>Translated_568_residues
MQQFVNSLSQPPAPDGAAHPFAGAWAQLMNQTNQLFALQSSLYQQQLNLWSQFLGQAAGQEAAAEAGAKPADRRFASPEW
NEHPFYNFLKQSYLQTSKWMMELVDKTQLDEDAKDKLAFATRQYLDAMSPSNFMLTNPDVVKRAIETKGESLVEGMKNML
DDFQKGHISMSDESKFEIGKNLVVTPGQVVFRNELIELIQYTPTTDKVYEKPLLFVPPCINKYYLMDLQPDNSMVRHFVA
QGYRVFLISWRSAVAEMKHFTWETYIEKGVFAAAEAVQKITKQPTMNVLGFCVGGVILTTALCVAQAKGLKYFDSATFMT
SLIDHAEPGEISFFIDESVVAGREAKMASGGIISGKEIGRTFASLRANDLVWNYVVNNYLLGKTPAPFDLLFWNNDAVDL
PLPMHTFLLRQFYMNNALVRPGAITLCGVPIDIAKIDVPVYMFAARDDHIVLWSSAFSGLKYLQGAPSRRFVLGASGHIA
GSINPVTKDKRNYWANDTLPLHAEEWLESAESRPGSWWKDWDAWLAPQSGKQVAAPKSLGNKEFPPLLAAPGSYVLAKAM
PSVAASLQ
>Mature_568_residues
MQQFVNSLSQPPAPDGAAHPFAGAWAQLMNQTNQLFALQSSLYQQQLNLWSQFLGQAAGQEAAAEAGAKPADRRFASPEW
NEHPFYNFLKQSYLQTSKWMMELVDKTQLDEDAKDKLAFATRQYLDAMSPSNFMLTNPDVVKRAIETKGESLVEGMKNML
DDFQKGHISMSDESKFEIGKNLVVTPGQVVFRNELIELIQYTPTTDKVYEKPLLFVPPCINKYYLMDLQPDNSMVRHFVA
QGYRVFLISWRSAVAEMKHFTWETYIEKGVFAAAEAVQKITKQPTMNVLGFCVGGVILTTALCVAQAKGLKYFDSATFMT
SLIDHAEPGEISFFIDESVVAGREAKMASGGIISGKEIGRTFASLRANDLVWNYVVNNYLLGKTPAPFDLLFWNNDAVDL
PLPMHTFLLRQFYMNNALVRPGAITLCGVPIDIAKIDVPVYMFAARDDHIVLWSSAFSGLKYLQGAPSRRFVLGASGHIA
GSINPVTKDKRNYWANDTLPLHAEEWLESAESRPGSWWKDWDAWLAPQSGKQVAAPKSLGNKEFPPLLAAPGSYVLAKAM
PSVAASLQ

Specific function: Involved in the production of polyhydroxyalkonic acids (PHAs), composed primarily of 3-hydroxybutyric acid (3HB) and 3- hydroxyvaleric acid (3HV)

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHAC_CHRVO (Q9ZHI2)

Other databases:

- EMBL:   AF061446
- EMBL:   AE016825
- RefSeq:   NP_902459.1
- ProteinModelPortal:   Q9ZHI2
- GeneID:   2547099
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2789
- NMPDR:   fig|243365.1.peg.2789
- HOGENOM:   HBG294095
- OMA:   GIEFANT
- ProtClustDB:   CLSK2485515
- BioCyc:   CVIO243365:CV_2789-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941
- TIGRFAMs:   TIGR01838

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N

EC number: NA

Molecular weight: Translated: 63271; Mature: 63271

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: ACT_SITE 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure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CCCCCCCEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10427049; 14500782