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The map label for this gene is ispA [H]

Identifier: 34498146

GI number: 34498146

Start: 2913197

End: 2914093

Strand: Direct

Name: ispA [H]

Synonym: CV_2691

Alternate gene names: 34498146

Gene position: 2913197-2914093 (Clockwise)

Preceding gene: 34498145

Following gene: 34498147

Centisome position: 61.32

GC content: 68.34

Gene sequence:

>897_bases
ATGGCCAATGAAAGCTTCATCGGCTGGATGACCTCCATCCAGCAATACATGGAACAGGCGCTGGAAAAGTTGCTGCCGTC
GGCTGACTGCGCCCCGCAACGGCTCCATGAAGCGATGCGCTACGCGACGCTGGAGGGCGGCAAGCGCGTGCGCCCGCTGC
TGGTGTTCGCCGCCGGCGAGCTGGTCGGCGCGCAAGCGGACAATCTGGCCCGCATCGGCGCGGCGCTGGAAATGGTCCAT
GCTTACTCGCTGGTCCACGACGACATGCCCTGCATGGACAACGACGTGCTGCGCCGCGGCAAGCCCACCTGTCACATCGC
CTACGATGACGCCACCGCGCTCCTGGTCGGCGACGCGCTGCAGACCCGCGCCTTCGAGCTGATCTCCCAGCCCATGCCGG
ACATCGCGCCGGCCAGCCAGCTGGAAATGGTCCGCGCGCTGGCCCACGCCGCCGGCCACGCCGGCATGGCGGGCGGCCAG
GCGATAGACCTGGCCAGCGTCGGCCTGCCGCTGACGCTGCCGGAGCTGGAATTCATGCACATGCTGAAGACCGGCGCGCT
GATCCGCGCCGCGGTGTTGGCGGGTTCCATGGCGGGCCGCGAGCTGTCCGCCGCAGAGAAGGAGGCGCTCGACGCCTTCG
CCAAGCGCATCGGCCTGGCCTTCCAGGTGGTGGACGACATTCTGGACTGCGAGGCGGACACCGCGACGCTGGGCAAGACC
GCCGGCAAGGACGCCGCCAACGACAAACCCACCTACGTCAGCCTGCTGGGCCTCGCCGAAGCCAAGCGGATGGCGAAGGA
TTTGCACGACCAGGCCTTCGCCGCGCTGGAGGGCTTCGGCCCGCAGGCCGACCGCCTGAAACAACTGGCCGACTACATCG
TCGCCCGCTCGTTTTGA

Upstream 100 bases:

>100_bases
GGCACCGAATTGATCAAGTTCTGCCAGGGCAAGCTGGCCGACGCGGAACAGCAGCTGAAAATCCTGGAAAACAACGAACT
GAAGACGCTGGATCTGCCCA

Downstream 100 bases:

>100_bases
ACCGCTCGTGGAGAAACCATCAGGACGGACCACCGCCCGGCGAGCCCTCTCACTGCAGAACCACAACAATGTATCCGCTG
CTTGAAAACATAAACAGCCC

Product: geranyltranstransferase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MANESFIGWMTSIQQYMEQALEKLLPSADCAPQRLHEAMRYATLEGGKRVRPLLVFAAGELVGAQADNLARIGAALEMVH
AYSLVHDDMPCMDNDVLRRGKPTCHIAYDDATALLVGDALQTRAFELISQPMPDIAPASQLEMVRALAHAAGHAGMAGGQ
AIDLASVGLPLTLPELEFMHMLKTGALIRAAVLAGSMAGRELSAAEKEALDAFAKRIGLAFQVVDDILDCEADTATLGKT
AGKDAANDKPTYVSLLGLAEAKRMAKDLHDQAFAALEGFGPQADRLKQLADYIVARSF

Sequences:

>Translated_298_residues
MANESFIGWMTSIQQYMEQALEKLLPSADCAPQRLHEAMRYATLEGGKRVRPLLVFAAGELVGAQADNLARIGAALEMVH
AYSLVHDDMPCMDNDVLRRGKPTCHIAYDDATALLVGDALQTRAFELISQPMPDIAPASQLEMVRALAHAAGHAGMAGGQ
AIDLASVGLPLTLPELEFMHMLKTGALIRAAVLAGSMAGRELSAAEKEALDAFAKRIGLAFQVVDDILDCEADTATLGKT
AGKDAANDKPTYVSLLGLAEAKRMAKDLHDQAFAALEGFGPQADRLKQLADYIVARSF
>Mature_297_residues
ANESFIGWMTSIQQYMEQALEKLLPSADCAPQRLHEAMRYATLEGGKRVRPLLVFAAGELVGAQADNLARIGAALEMVHA
YSLVHDDMPCMDNDVLRRGKPTCHIAYDDATALLVGDALQTRAFELISQPMPDIAPASQLEMVRALAHAAGHAGMAGGQA
IDLASVGLPLTLPELEFMHMLKTGALIRAAVLAGSMAGRELSAAEKEALDAFAKRIGLAFQVVDDILDCEADTATLGKTA
GKDAANDKPTYVSLLGLAEAKRMAKDLHDQAFAALEGFGPQADRLKQLADYIVARSF

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786623, Length=284, Percent_Identity=46.4788732394366, Blast_Score=217, Evalue=9e-58,
Organism=Escherichia coli, GI1789578, Length=231, Percent_Identity=31.6017316017316, Blast_Score=110, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 31846; Mature: 31715

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANESFIGWMTSIQQYMEQALEKLLPSADCAPQRLHEAMRYATLEGGKRVRPLLVFAAGE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHH
LVGAQADNLARIGAALEMVHAYSLVHDDMPCMDNDVLRRGKPTCHIAYDDATALLVGDAL
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEEECCCHHHHHHHHH
QTRAFELISQPMPDIAPASQLEMVRALAHAAGHAGMAGGQAIDLASVGLPLTLPELEFMH
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEHHHCCCCCCCHHHHHHH
MLKTGALIRAAVLAGSMAGRELSAAEKEALDAFAKRIGLAFQVVDDILDCEADTATLGKT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
AGKDAANDKPTYVSLLGLAEAKRMAKDLHDQAFAALEGFGPQADRLKQLADYIVARSF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANESFIGWMTSIQQYMEQALEKLLPSADCAPQRLHEAMRYATLEGGKRVRPLLVFAAGE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHH
LVGAQADNLARIGAALEMVHAYSLVHDDMPCMDNDVLRRGKPTCHIAYDDATALLVGDAL
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEEECCCHHHHHHHHH
QTRAFELISQPMPDIAPASQLEMVRALAHAAGHAGMAGGQAIDLASVGLPLTLPELEFMH
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEHHHCCCCCCCHHHHHHH
MLKTGALIRAAVLAGSMAGRELSAAEKEALDAFAKRIGLAFQVVDDILDCEADTATLGKT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
AGKDAANDKPTYVSLLGLAEAKRMAKDLHDQAFAALEGFGPQADRLKQLADYIVARSF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515931 [H]