Click here to switch to the map view.

The map label for this gene is iciA [H]

Identifier: 34498024

GI number: 34498024

Start: 2771502

End: 2772440

Strand: Direct

Name: iciA [H]

Synonym: CV_2569

Alternate gene names: 34498024

Gene position: 2771502-2772440 (Clockwise)

Preceding gene: 34498021

Following gene: 34498025

Centisome position: 58.33

GC content: 66.13

Gene sequence:

>939_bases
GTGTTCGACCCCAGACAATTACAGACCCTGGCCGCAGTGGCGGAAACCGGGGGCTTCGACAAAGCCGCCAAGAAGCTGTT
TCTGACCCAATCCGCGGTATCGCAACGCATACGCCAGCTGGAGGAACAACTGGGCCAGCCCGTGCTGACCCGCACCTCGC
CGGCAGAGGCCACCGAGGCCGGGCGCCAGCTGCTGCGGCATTATCAGCAGTTGTTGCTGATGGAAAACCAGCTGCTGCAA
AAACTCAGTCCCGCGCCGCGCCAGCGCGAATTCACCACGCTCGCCGTCGGCGTCAACGCCGACAGCCTGGCCACCTGGTA
TCTGCAGGCGGTGACGCCGGTGCTGGAAGCCCACGACCTGTTGCTGGACGTGGTGGTCGACGACCAGGACCACACCCATG
AACTGATGCGCACCGGCCACGTGATGGGCTGCGTCAGCACCCGGCCGGTGCCGATCCAGACCGGCGTCCAGCATTACCTG
GGCGCGATGCGCTATCTGTGCCTGGCCACGCCGCGCTTCGCCGCGCGGCATTTTCCGGACGGCGTCGACCACGCGGCGCT
GGCGCGCGCGCCCAGCATCATCTTCAGCCGCAAGGACGCGGTGCACGGCCAGTTCCTGCGCCAGGCGATCGGCTACGAGG
GCGGCTTTCCCAGCTTCACCGTGCCCTCGGCCCAGGGCTTCGTCGAACTCACCCGCCAGGGCGTCGCCTACAGCCTGCTG
CCGGAGCTGATGCTGGACGGCGACCTGGAAGACGGCCGGCTGGTGGACCTGTTCCCCGGCCAATACATGGACCTGCCGCT
GTACTGGCACCACTGGCGGGTGGAATCCGAGCTGTCCCAGGCTTTGACCGAGGCCATGCTGGACTACTGCGGCCGCCACT
TGCGCCAGGACAAAGTGGCATATCATGCATCTGTTGGAGAATCACGGCAGAACCCCTGA

Upstream 100 bases:

>100_bases
CGGCCGCCAAAGCCTGCAACATGCCACGGCGCTGGCTTTTAAGCAAGCCCGGCGGCATTATTAAACGACCTCTTATCGTC
AAAAGACCAGGGACCGCGAA

Downstream 100 bases:

>100_bases
CCCAAGCGGCGCGGCTGTCGCATTGACAAGGCCACGCCCCCTGGCGCATCATCCGGCGCCGACCTGGTTTTATGGAGGCA
GCATGACCAGCAAGCAGCAA

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MFDPRQLQTLAAVAETGGFDKAAKKLFLTQSAVSQRIRQLEEQLGQPVLTRTSPAEATEAGRQLLRHYQQLLLMENQLLQ
KLSPAPRQREFTTLAVGVNADSLATWYLQAVTPVLEAHDLLLDVVVDDQDHTHELMRTGHVMGCVSTRPVPIQTGVQHYL
GAMRYLCLATPRFAARHFPDGVDHAALARAPSIIFSRKDAVHGQFLRQAIGYEGGFPSFTVPSAQGFVELTRQGVAYSLL
PELMLDGDLEDGRLVDLFPGQYMDLPLYWHHWRVESELSQALTEAMLDYCGRHLRQDKVAYHASVGESRQNP

Sequences:

>Translated_312_residues
MFDPRQLQTLAAVAETGGFDKAAKKLFLTQSAVSQRIRQLEEQLGQPVLTRTSPAEATEAGRQLLRHYQQLLLMENQLLQ
KLSPAPRQREFTTLAVGVNADSLATWYLQAVTPVLEAHDLLLDVVVDDQDHTHELMRTGHVMGCVSTRPVPIQTGVQHYL
GAMRYLCLATPRFAARHFPDGVDHAALARAPSIIFSRKDAVHGQFLRQAIGYEGGFPSFTVPSAQGFVELTRQGVAYSLL
PELMLDGDLEDGRLVDLFPGQYMDLPLYWHHWRVESELSQALTEAMLDYCGRHLRQDKVAYHASVGESRQNP
>Mature_312_residues
MFDPRQLQTLAAVAETGGFDKAAKKLFLTQSAVSQRIRQLEEQLGQPVLTRTSPAEATEAGRQLLRHYQQLLLMENQLLQ
KLSPAPRQREFTTLAVGVNADSLATWYLQAVTPVLEAHDLLLDVVVDDQDHTHELMRTGHVMGCVSTRPVPIQTGVQHYL
GAMRYLCLATPRFAARHFPDGVDHAALARAPSIIFSRKDAVHGQFLRQAIGYEGGFPSFTVPSAQGFVELTRQGVAYSLL
PELMLDGDLEDGRLVDLFPGQYMDLPLYWHHWRVESELSQALTEAMLDYCGRHLRQDKVAYHASVGESRQNP

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=298, Percent_Identity=41.2751677852349, Blast_Score=218, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34911; Mature: 34911

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDPRQLQTLAAVAETGGFDKAAKKLFLTQSAVSQRIRQLEEQLGQPVLTRTSPAEATEA
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHH
GRQLLRHYQQLLLMENQLLQKLSPAPRQREFTTLAVGVNADSLATWYLQAVTPVLEAHDL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
LLDVVVDDQDHTHELMRTGHVMGCVSTRPVPIQTGVQHYLGAMRYLCLATPRFAARHFPD
HHHHEECCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
GVDHAALARAPSIIFSRKDAVHGQFLRQAIGYEGGFPSFTVPSAQGFVELTRQGVAYSLL
CCCHHHHHHCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCHHHHHH
PELMLDGDLEDGRLVDLFPGQYMDLPLYWHHWRVESELSQALTEAMLDYCGRHLRQDKVA
HHHHHCCCCCCCEEEEECCCCEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YHASVGESRQNP
HHHHCCCCCCCC
>Mature Secondary Structure
MFDPRQLQTLAAVAETGGFDKAAKKLFLTQSAVSQRIRQLEEQLGQPVLTRTSPAEATEA
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHH
GRQLLRHYQQLLLMENQLLQKLSPAPRQREFTTLAVGVNADSLATWYLQAVTPVLEAHDL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
LLDVVVDDQDHTHELMRTGHVMGCVSTRPVPIQTGVQHYLGAMRYLCLATPRFAARHFPD
HHHHEECCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
GVDHAALARAPSIIFSRKDAVHGQFLRQAIGYEGGFPSFTVPSAQGFVELTRQGVAYSLL
CCCHHHHHHCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCHHHHHH
PELMLDGDLEDGRLVDLFPGQYMDLPLYWHHWRVESELSQALTEAMLDYCGRHLRQDKVA
HHHHHCCCCCCCEEEEECCCCEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YHASVGESRQNP
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA