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The map label for this gene is narX [H]

Identifier: 34497989

GI number: 34497989

Start: 2725451

End: 2727349

Strand: Direct

Name: narX [H]

Synonym: CV_2534

Alternate gene names: 34497989

Gene position: 2725451-2727349 (Clockwise)

Preceding gene: 34497987

Following gene: 34497990

Centisome position: 57.36

GC content: 66.4

Gene sequence:

>1899_bases
ATGCCCGACATCCGTTTTGCCCCTCCCCGCTTCCAGACCCTGGCCAGCAAGCTGCTGGTGTTGTCGCTGCTGGCGCTGTG
CGTGGCGCTGATCTCGATAGGCTATACCTTGGCGCTGTCCTGGCGGCTGGAAGGCGGCGCCGCCGCAATCAACGATGTGG
GCAGCCTGCGCATGCGCACTTTCCGCGCCGCCTACCTGATGTCCACCCAAGCCCCCGAGCGCGTGCTGCGCGACGAGGTC
GCCGGCTTTGACCAGACCCTGGCCCGGCTGAAGCGCGGCGATCCGGCGCGGCCGCTGTTCCTACCCGACAACCGCGATAT
CCGCGACCAGGAACGGCGGCTGGAGCAGCGCTGGCGCAACGATATCCGCCCGCTGCTGCTGGCCGCCGACGGCGCCCAGC
CGTCCGCGCAGCGGCTGCACGGCTTCGTCGCCGAGATAGACAACCTGGTCAGCCTGCTGGAGCGCGACAACGAGAACCAC
ACCAATCTGCTGCGGATGTTCCAGATGGTGCTGATCGCGATGGCGCTGGCCGGCGCGGTGACCATGGCTTACATGCTGTT
CCTGATGGTGATCAACCCGGTCCGCGCGCTCAGCGACGCGATGCGCCGGCTGCGCGAGGGCGAGCTGGACACCCGGGTCA
AAGTGGATCGCCAGGACGAGCTCGGCATGCTGGCCGAGGGCTTCAACCAGATGGCCGAGCGGGCGCAGGACCTGTACCAG
AACCTGGAGAGCAAGGTCGCCCAAAAAACTCGCGAGGTGGAAGAACAGAACCTGCGGCTGAAAACGCTGTACGACATGAC
TTCCTTCCTGCACCAGCAGCGCGCGCTGGACGAAACCTGCCAGGGCTTCATCCGCCGCCTGATGCAGCTGACCGGCGCCG
ACGCGGCCAACGCCAGGCTGGTGGACCCGGAGCGCGGCAAGCTGGACCAGATCGCCCAGCAAGGCCTGCCCGCGGAGATG
GCGCTCAACGAGGAATGCATCCCCGACGACGCCTGCCACTGCGGCGAAGCGGTGCAGCAGCCCTTCGCCGTGCTGCGCCA
CATCGGCGAGCTGGAAGGCGAGGAAATCCGCCATTGCCGCAAGGCGGGCTTCGCCTCCATCGCCGTGTTCCACATCCGCA
ACCAGCGCGAGGATGTCGGCATCTTCACGCTTTACTTCCGCCAGAAGCGCGTGCTGTCCATGGCCGAGCAGATGCTGATC
GAGACCCTGGGCCAGCACCTGGGCGTGGCCATCGAAAACCAGCGGCTGGGCGCGGTGGAACGCCAGTTCGCGGTGTCCGA
GGAGCGCAACCTGATGGCCCAGGGCCTGCACGACAGCATCGCCCAGTCGCTGTCCTTCCTCAATCTGCAGACGCAGATGC
TGGAAGACGCGATGAATTCGCGCGAGGAGGCGCTGGCGCGCGAAAACCTGGCCTTCATCCGCGCCGGCGTGCAGGAGTGC
TACGAGGACGTCAGGGAGCTGCTGCTCAACTTCCGCACCCGCATCAGCCAGCAGGAATTCCCCGAGGCGGTGCGCACGCT
GCTGCAGCGCTTCGAACAGCAGACCCGGGTGCCGGCGCGGCTGGAAATGCGCGGCGAAGGCAAGCCGCTGGACCCGCAGC
AGCAGCTGCAGGTGTTTTTCATCCTGCAGGAAGCGCTGTCCAACATCCGCAAGCACGCCGAAGCCAGCGAGGTGGAAGTG
GAGATCGACAACACCGCCAGCTTCAGCATGCGCATCCGCGACAACGGCCGCGGCTTCGATTCCGGCCAGCTGGCGGCGAA
ATCCGCGCGCCACGTCGGCATGTCCATCATGCAAGAACGGGCCAAACGCATACACAGCCATATCCAGATCCGCTCGGCGC
CCACGCAGGGCACCACGGTGGAGCTGACCCTGCCCAAAGAGGAGCGCATCAGCGCATGA

Upstream 100 bases:

>100_bases
AAGCTATGTTTAAACAATTCATATCTGAACCATAACCGCGTCCAGACATTGTGCAGTCCGCCGCCGCCGTTTGCCATCCG
CCAAAAGCAGAACCATGCCC

Downstream 100 bases:

>100_bases
CACAAGCCATACGCATTCTGTTGATTGACGATCACACGCTGTTCCGCAGCGGCCTGAAGGCGCTGCTGCAGCGGCAGGAC
GATTTCCTGGTGGTCGGCGA

Product: transmembrane nitrate/nitrite sensor kinase transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MPDIRFAPPRFQTLASKLLVLSLLALCVALISIGYTLALSWRLEGGAAAINDVGSLRMRTFRAAYLMSTQAPERVLRDEV
AGFDQTLARLKRGDPARPLFLPDNRDIRDQERRLEQRWRNDIRPLLLAADGAQPSAQRLHGFVAEIDNLVSLLERDNENH
TNLLRMFQMVLIAMALAGAVTMAYMLFLMVINPVRALSDAMRRLREGELDTRVKVDRQDELGMLAEGFNQMAERAQDLYQ
NLESKVAQKTREVEEQNLRLKTLYDMTSFLHQQRALDETCQGFIRRLMQLTGADAANARLVDPERGKLDQIAQQGLPAEM
ALNEECIPDDACHCGEAVQQPFAVLRHIGELEGEEIRHCRKAGFASIAVFHIRNQREDVGIFTLYFRQKRVLSMAEQMLI
ETLGQHLGVAIENQRLGAVERQFAVSEERNLMAQGLHDSIAQSLSFLNLQTQMLEDAMNSREEALARENLAFIRAGVQEC
YEDVRELLLNFRTRISQQEFPEAVRTLLQRFEQQTRVPARLEMRGEGKPLDPQQQLQVFFILQEALSNIRKHAEASEVEV
EIDNTASFSMRIRDNGRGFDSGQLAAKSARHVGMSIMQERAKRIHSHIQIRSAPTQGTTVELTLPKEERISA

Sequences:

>Translated_632_residues
MPDIRFAPPRFQTLASKLLVLSLLALCVALISIGYTLALSWRLEGGAAAINDVGSLRMRTFRAAYLMSTQAPERVLRDEV
AGFDQTLARLKRGDPARPLFLPDNRDIRDQERRLEQRWRNDIRPLLLAADGAQPSAQRLHGFVAEIDNLVSLLERDNENH
TNLLRMFQMVLIAMALAGAVTMAYMLFLMVINPVRALSDAMRRLREGELDTRVKVDRQDELGMLAEGFNQMAERAQDLYQ
NLESKVAQKTREVEEQNLRLKTLYDMTSFLHQQRALDETCQGFIRRLMQLTGADAANARLVDPERGKLDQIAQQGLPAEM
ALNEECIPDDACHCGEAVQQPFAVLRHIGELEGEEIRHCRKAGFASIAVFHIRNQREDVGIFTLYFRQKRVLSMAEQMLI
ETLGQHLGVAIENQRLGAVERQFAVSEERNLMAQGLHDSIAQSLSFLNLQTQMLEDAMNSREEALARENLAFIRAGVQEC
YEDVRELLLNFRTRISQQEFPEAVRTLLQRFEQQTRVPARLEMRGEGKPLDPQQQLQVFFILQEALSNIRKHAEASEVEV
EIDNTASFSMRIRDNGRGFDSGQLAAKSARHVGMSIMQERAKRIHSHIQIRSAPTQGTTVELTLPKEERISA
>Mature_631_residues
PDIRFAPPRFQTLASKLLVLSLLALCVALISIGYTLALSWRLEGGAAAINDVGSLRMRTFRAAYLMSTQAPERVLRDEVA
GFDQTLARLKRGDPARPLFLPDNRDIRDQERRLEQRWRNDIRPLLLAADGAQPSAQRLHGFVAEIDNLVSLLERDNENHT
NLLRMFQMVLIAMALAGAVTMAYMLFLMVINPVRALSDAMRRLREGELDTRVKVDRQDELGMLAEGFNQMAERAQDLYQN
LESKVAQKTREVEEQNLRLKTLYDMTSFLHQQRALDETCQGFIRRLMQLTGADAANARLVDPERGKLDQIAQQGLPAEMA
LNEECIPDDACHCGEAVQQPFAVLRHIGELEGEEIRHCRKAGFASIAVFHIRNQREDVGIFTLYFRQKRVLSMAEQMLIE
TLGQHLGVAIENQRLGAVERQFAVSEERNLMAQGLHDSIAQSLSFLNLQTQMLEDAMNSREEALARENLAFIRAGVQECY
EDVRELLLNFRTRISQQEFPEAVRTLLQRFEQQTRVPARLEMRGEGKPLDPQQQLQVFFILQEALSNIRKHAEASEVEVE
IDNTASFSMRIRDNGRGFDSGQLAAKSARHVGMSIMQERAKRIHSHIQIRSAPTQGTTVELTLPKEERISA

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the narL protein and of both nitrate/nitrite to the narP protein. NarX probably activates narL and narP by phosphorylation in the presence of nitrate. NarX also plays a

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=629, Percent_Identity=26.7090620031796, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1788812, Length=620, Percent_Identity=24.3548387096774, Blast_Score=112, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR003661
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 71777; Mature: 71646

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDIRFAPPRFQTLASKLLVLSLLALCVALISIGYTLALSWRLEGGAAAINDVGSLRMRT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHH
FRAAYLMSTQAPERVLRDEVAGFDQTLARLKRGDPARPLFLPDNRDIRDQERRLEQRWRN
HHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHH
DIRPLLLAADGAQPSAQRLHGFVAEIDNLVSLLERDNENHTNLLRMFQMVLIAMALAGAV
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
TMAYMLFLMVINPVRALSDAMRRLREGELDTRVKVDRQDELGMLAEGFNQMAERAQDLYQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
NLESKVAQKTREVEEQNLRLKTLYDMTSFLHQQRALDETCQGFIRRLMQLTGADAANARL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VDPERGKLDQIAQQGLPAEMALNEECIPDDACHCGEAVQQPFAVLRHIGELEGEEIRHCR
CCCCCCCHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KAGFASIAVFHIRNQREDVGIFTLYFRQKRVLSMAEQMLIETLGQHLGVAIENQRLGAVE
HCCHHHEEHEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHH
RQFAVSEERNLMAQGLHDSIAQSLSFLNLQTQMLEDAMNSREEALARENLAFIRAGVQEC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEDVRELLLNFRTRISQQEFPEAVRTLLQRFEQQTRVPARLEMRGEGKPLDPQQQLQVFF
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHH
ILQEALSNIRKHAEASEVEVEIDNTASFSMRIRDNGRGFDSGQLAAKSARHVGMSIMQER
HHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
AKRIHSHIQIRSAPTQGTTVELTLPKEERISA
HHHHHHHHEEECCCCCCCEEEEECCCHHCCCC
>Mature Secondary Structure 
PDIRFAPPRFQTLASKLLVLSLLALCVALISIGYTLALSWRLEGGAAAINDVGSLRMRT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHH
FRAAYLMSTQAPERVLRDEVAGFDQTLARLKRGDPARPLFLPDNRDIRDQERRLEQRWRN
HHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHH
DIRPLLLAADGAQPSAQRLHGFVAEIDNLVSLLERDNENHTNLLRMFQMVLIAMALAGAV
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
TMAYMLFLMVINPVRALSDAMRRLREGELDTRVKVDRQDELGMLAEGFNQMAERAQDLYQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
NLESKVAQKTREVEEQNLRLKTLYDMTSFLHQQRALDETCQGFIRRLMQLTGADAANARL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VDPERGKLDQIAQQGLPAEMALNEECIPDDACHCGEAVQQPFAVLRHIGELEGEEIRHCR
CCCCCCCHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KAGFASIAVFHIRNQREDVGIFTLYFRQKRVLSMAEQMLIETLGQHLGVAIENQRLGAVE
HCCHHHEEHEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHH
RQFAVSEERNLMAQGLHDSIAQSLSFLNLQTQMLEDAMNSREEALARENLAFIRAGVQEC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEDVRELLLNFRTRISQQEFPEAVRTLLQRFEQQTRVPARLEMRGEGKPLDPQQQLQVFF
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHH
ILQEALSNIRKHAEASEVEVEIDNTASFSMRIRDNGRGFDSGQLAAKSARHVGMSIMQER
HHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
AKRIHSHIQIRSAPTQGTTVELTLPKEERISA
HHHHHHHHEEECCCCCCCEEEEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]