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The map label for this gene is moaA2 [H]

Identifier: 34497987

GI number: 34497987

Start: 2723842

End: 2724816

Strand: Direct

Name: moaA2 [H]

Synonym: CV_2532

Alternate gene names: 34497987

Gene position: 2723842-2724816 (Clockwise)

Preceding gene: 34497985

Following gene: 34497989

Centisome position: 57.33

GC content: 70.15

Gene sequence:

>975_bases
ATGTTGTCCGATCGCTTCGGCCGCGCCATCGATTACCTGCGGCTTTCCGTCACCGACCGCTGCGACCTGCGTTGCAGCTA
TTGCCTGCCCAAGGGCTTCAAGGGCTTCGAGGAGCCCGCCCACTGGCTGACTTTCGACGAGATCGAACGCGTCGCCGCGG
CCTTCGTCCGCCTGGGCGCGCGCCGCATCCGCCTGACCGGCGGCGAGCCGCTGCTGCGCCGCGAGCTGCCGCTGCTGGCC
GGACGGCTGTCCGCCCTGCCCGGCCTGGCCGACCTGTCGCTGTCCACCAACGCCACCCAGCTGGAGCGCCACGCCAAGGC
GCTGCGCCAGGCGGGCGTGAAGCGGCTCAACGTCAGCCTGGACTCGCTGCGCCGCGACTGCGTGGCGGAGCTGACCGGCA
GCGACAGCTTGCCCAAGGCGCTGGCGGGCCTGTCGGCGGCGAAAGCCGCCGGTTTCGAGCGCATCAAGATCAATATGGTG
CCGATAGCCGGGGTCAACGACGGCGACATCGAGGCGATGGCGGAGTTCTGCATCGAGCACGGCTTCATCCTGCGCCTGAT
CGAGGCGATGCCGATGGGCGACAGCGGCCGCGGCGCGCGCGGCAGCGAGCTGGCGCCGATATTGGAGCGGCTGGGCCCCC
GCTTCGGCCTGTCCGAGCTCAGCGTCAGCCTGGGCGGCGGACCGGCGCGCTATTGGGGATCGAGCGACGGGCGCTTCACG
CTGGGCCTGATCACGCCGATCAGCCAGCACTTCTGCGCCAGCTGCAACCGCTTGCGGATGTCGGTCGACGGCACGCTCTA
CCTGTGTCTGGGCCAGGAGCAGCGCTTCGAGCTGCGGCCGCTGCTGCGCGCCGGCTGCAGCGACGCCGAGCTGGAAGCCG
CCATCCGCGCCGCCATCGAGCTGAAGCCGGAAAAACACGACTTCCTGGAAGCGCCGGCCAAGGTGATGCGCTTCATGTCC
CAGACCGGCGGCTGA

Upstream 100 bases:

>100_bases
TACGACTAAATAGCTAGTCCGCCTCCCACCTCCAGGGAACTGCCGAGCGCCCATGCCCGGCATACAATCCAGACATACCG
GCAGTCCCCGAGGATGCACC

Downstream 100 bases:

>100_bases
CTCAGCGCGTATCGGCCAGCCAGCCAGCGATGAAATGCGCCACCGCGTCCGCGTCGTTCAGATCCAGCACCGGGATGCCG
GCCTGCAGCGGCGCGTCGCA

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MLSDRFGRAIDYLRLSVTDRCDLRCSYCLPKGFKGFEEPAHWLTFDEIERVAAAFVRLGARRIRLTGGEPLLRRELPLLA
GRLSALPGLADLSLSTNATQLERHAKALRQAGVKRLNVSLDSLRRDCVAELTGSDSLPKALAGLSAAKAAGFERIKINMV
PIAGVNDGDIEAMAEFCIEHGFILRLIEAMPMGDSGRGARGSELAPILERLGPRFGLSELSVSLGGGPARYWGSSDGRFT
LGLITPISQHFCASCNRLRMSVDGTLYLCLGQEQRFELRPLLRAGCSDAELEAAIRAAIELKPEKHDFLEAPAKVMRFMS
QTGG

Sequences:

>Translated_324_residues
MLSDRFGRAIDYLRLSVTDRCDLRCSYCLPKGFKGFEEPAHWLTFDEIERVAAAFVRLGARRIRLTGGEPLLRRELPLLA
GRLSALPGLADLSLSTNATQLERHAKALRQAGVKRLNVSLDSLRRDCVAELTGSDSLPKALAGLSAAKAAGFERIKINMV
PIAGVNDGDIEAMAEFCIEHGFILRLIEAMPMGDSGRGARGSELAPILERLGPRFGLSELSVSLGGGPARYWGSSDGRFT
LGLITPISQHFCASCNRLRMSVDGTLYLCLGQEQRFELRPLLRAGCSDAELEAAIRAAIELKPEKHDFLEAPAKVMRFMS
QTGG
>Mature_324_residues
MLSDRFGRAIDYLRLSVTDRCDLRCSYCLPKGFKGFEEPAHWLTFDEIERVAAAFVRLGARRIRLTGGEPLLRRELPLLA
GRLSALPGLADLSLSTNATQLERHAKALRQAGVKRLNVSLDSLRRDCVAELTGSDSLPKALAGLSAAKAAGFERIKINMV
PIAGVNDGDIEAMAEFCIEHGFILRLIEAMPMGDSGRGARGSELAPILERLGPRFGLSELSVSLGGGPARYWGSSDGRFT
LGLITPISQHFCASCNRLRMSVDGTLYLCLGQEQRFELRPLLRAGCSDAELEAAIRAAIELKPEKHDFLEAPAKVMRFMS
QTGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=307, Percent_Identity=32.8990228013029, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI22027536, Length=307, Percent_Identity=32.8990228013029, Blast_Score=167, Evalue=1e-41,
Organism=Escherichia coli, GI1786999, Length=327, Percent_Identity=37.0030581039755, Blast_Score=204, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI32566331, Length=323, Percent_Identity=32.1981424148607, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI32566329, Length=315, Percent_Identity=32.0634920634921, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17561210, Length=310, Percent_Identity=30.6451612903226, Blast_Score=171, Evalue=5e-43,
Organism=Drosophila melanogaster, GI28574490, Length=313, Percent_Identity=34.8242811501597, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI28574488, Length=313, Percent_Identity=34.8242811501597, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 35315; Mature: 35315

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS01305 MOAA_NIFB_PQQE ; PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSDRFGRAIDYLRLSVTDRCDLRCSYCLPKGFKGFEEPAHWLTFDEIERVAAAFVRLGA
CCCCHHHHHHHHHHEECCCCCCCEEEECCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHCC
RRIRLTGGEPLLRRELPLLAGRLSALPGLADLSLSTNATQLERHAKALRQAGVKRLNVSL
CEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHEECCCH
DSLRRDCVAELTGSDSLPKALAGLSAAKAAGFERIKINMVPIAGVNDGDIEAMAEFCIEH
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHHC
GFILRLIEAMPMGDSGRGARGSELAPILERLGPRFGLSELSVSLGGGPARYWGSSDGRFT
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHEEECCCCCCCCCCCCCCCEE
LGLITPISQHFCASCNRLRMSVDGTLYLCLGQEQRFELRPLLRAGCSDAELEAAIRAAIE
EEECCHHHHHHHHHHHHEEEEECCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHH
LKPEKHDFLEAPAKVMRFMSQTGG
CCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSDRFGRAIDYLRLSVTDRCDLRCSYCLPKGFKGFEEPAHWLTFDEIERVAAAFVRLGA
CCCCHHHHHHHHHHEECCCCCCCEEEECCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHCC
RRIRLTGGEPLLRRELPLLAGRLSALPGLADLSLSTNATQLERHAKALRQAGVKRLNVSL
CEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHEECCCH
DSLRRDCVAELTGSDSLPKALAGLSAAKAAGFERIKINMVPIAGVNDGDIEAMAEFCIEH
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHHC
GFILRLIEAMPMGDSGRGARGSELAPILERLGPRFGLSELSVSLGGGPARYWGSSDGRFT
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHEEECCCCCCCCCCCCCCCEE
LGLITPISQHFCASCNRLRMSVDGTLYLCLGQEQRFELRPLLRAGCSDAELEAAIRAAIE
EEECCHHHHHHHHHHHHEEEEECCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHH
LKPEKHDFLEAPAKVMRFMSQTGG
CCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA