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The map label for this gene is afsQ1 [H]

Identifier: 34497854

GI number: 34497854

Start: 2582034

End: 2583122

Strand: Direct

Name: afsQ1 [H]

Synonym: CV_2399

Alternate gene names: 34497854

Gene position: 2582034-2583122 (Clockwise)

Preceding gene: 34497849

Following gene: 34497858

Centisome position: 54.35

GC content: 65.2

Gene sequence:

>1089_bases
ATGGAAAAACACAGGATGCTGGTAATCGATGACGACCCGATCGCCCACCAGATCATGCAGATGCTGTTCGAGGCGGAGCT
GAGGCTGACTTGCAAGGTGACCGGAGAGGAAGGCTTGAGTGCCGTGGAAGAGGAGCAGCCGGAAATCGTGCTGCTGGACA
TCGAGATGGAAGGCATGGACGGCTACGAGGCTTGCCGCCGCATCCGCCAGCTGAATCTGCCGTCCCAGCCGTACATCATC
TTCGTATCGGCGCACAGCGACCCCGTCAAGCGTCTGGCCGCCTACAGCGTGGGAGGCGAGGATTTCGTCAGCAAGCCGCT
TCAGCCCCAGGAGCTGACGCGGAAGGTGGAGCAGGCGCTGCGCTATCGGCGCGAGATGCATGAGTTGAAAAGCCAGGCGC
AGGGCGCGATGTCGGTGGCGATGTCGGCCATGACGGACAGCAGCGCGCTGGGCATCTGTCTGCAGTATTTCCGCCGGCTG
TTCCAGGCGGCCGATCCAGGCCAACTGATGCAAATCACGCTGGACGCGCTGGGCGAGTTCGGCCTGGCCGCGGCGGTGCA
GTTCCGCGACGACGGAAAGGTGGAAACGCTGAGCTCGGCGCCCGACAGCCAGCTGGAGTCGCTGCTGCTGCAGGATCTGT
CGGCGGAGCCGCAACGGATCGTGAGCTATGACAGCCGCTGCGCGTTCAACTACGGGCCGATTTCCTTGCTGGTCAACAAT
ATGCCTGTTGACGACGAAGAACGCTGCGGCAAGTTGAAGGACTACCTGGCCTTGATGGCGGAAGGCGCTGCGGAGCGGGC
GAACGGAATGATCCGGGATGGCCGGCCTGACCGCCTGGACCAGATCCGCGACACGCTGGCCGCGATCGCCGCGAACCAGC
GCGCCAGCCAGTACGACGCGCGCGCGCTGCTGGACGCGATGAGGGCGCAACAGGAGGACCTGCTGGCCGCAATGGGCGTC
AGCGAGGCCCAGGCGGCGCCGTTGCTGGCCGTGGCGTGCGAGGCGCAGGCCGCGCTGGACAGGCTGCTTGGCCGGCAGAT
GAGAGAGCTGGAGCTGTTGAGCCTGCGCCTGACGGATGCGGGACGCTAG

Upstream 100 bases:

>100_bases
CGCCGATGGAAGAGCTATAGCTGTAATACCGGGATATCGCTGCGGTCGCGGGGCGTGCGCTCCGCAAGTTCAAGCTTAGT
CAAGCGAAAGGGGGGATGAA

Downstream 100 bases:

>100_bases
CGCGCCGCTGCGGCGCTCATGGCGAAGGCGGAGCGGCGTTCAGCAGGCAGTCGGCCTCTTCCTGCAGGGCGCGCAGCAAG
GCCTGATCCGGCTGGGCGTC

Product: two-component system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MEKHRMLVIDDDPIAHQIMQMLFEAELRLTCKVTGEEGLSAVEEEQPEIVLLDIEMEGMDGYEACRRIRQLNLPSQPYII
FVSAHSDPVKRLAAYSVGGEDFVSKPLQPQELTRKVEQALRYRREMHELKSQAQGAMSVAMSAMTDSSALGICLQYFRRL
FQAADPGQLMQITLDALGEFGLAAAVQFRDDGKVETLSSAPDSQLESLLLQDLSAEPQRIVSYDSRCAFNYGPISLLVNN
MPVDDEERCGKLKDYLALMAEGAAERANGMIRDGRPDRLDQIRDTLAAIAANQRASQYDARALLDAMRAQQEDLLAAMGV
SEAQAAPLLAVACEAQAALDRLLGRQMRELELLSLRLTDAGR

Sequences:

>Translated_362_residues
MEKHRMLVIDDDPIAHQIMQMLFEAELRLTCKVTGEEGLSAVEEEQPEIVLLDIEMEGMDGYEACRRIRQLNLPSQPYII
FVSAHSDPVKRLAAYSVGGEDFVSKPLQPQELTRKVEQALRYRREMHELKSQAQGAMSVAMSAMTDSSALGICLQYFRRL
FQAADPGQLMQITLDALGEFGLAAAVQFRDDGKVETLSSAPDSQLESLLLQDLSAEPQRIVSYDSRCAFNYGPISLLVNN
MPVDDEERCGKLKDYLALMAEGAAERANGMIRDGRPDRLDQIRDTLAAIAANQRASQYDARALLDAMRAQQEDLLAAMGV
SEAQAAPLLAVACEAQAALDRLLGRQMRELELLSLRLTDAGR
>Mature_362_residues
MEKHRMLVIDDDPIAHQIMQMLFEAELRLTCKVTGEEGLSAVEEEQPEIVLLDIEMEGMDGYEACRRIRQLNLPSQPYII
FVSAHSDPVKRLAAYSVGGEDFVSKPLQPQELTRKVEQALRYRREMHELKSQAQGAMSVAMSAMTDSSALGICLQYFRRL
FQAADPGQLMQITLDALGEFGLAAAVQFRDDGKVETLSSAPDSQLESLLLQDLSAEPQRIVSYDSRCAFNYGPISLLVNN
MPVDDEERCGKLKDYLALMAEGAAERANGMIRDGRPDRLDQIRDTLAAIAANQRASQYDARALLDAMRAQQEDLLAAMGV
SEAQAAPLLAVACEAQAALDRLLGRQMRELELLSLRLTDAGR

Specific function: Forms part of a two-component regulatory system AfsQ1/AfsQ2 involved in secondary metabolism [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=130, Percent_Identity=30, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790299, Length=132, Percent_Identity=32.5757575757576, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790860, Length=116, Percent_Identity=30.1724137931034, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789809, Length=129, Percent_Identity=29.4573643410853, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 40122; Mature: 40122

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKHRMLVIDDDPIAHQIMQMLFEAELRLTCKVTGEEGLSAVEEEQPEIVLLDIEMEGMD
CCCCCEEEECCCHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHHCCCCEEEEEEEECCCC
GYEACRRIRQLNLPSQPYIIFVSAHSDPVKRLAAYSVGGEDFVSKPLQPQELTRKVEQAL
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHH
RYRREMHELKSQAQGAMSVAMSAMTDSSALGICLQYFRRLFQAADPGQLMQITLDALGEF
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
GLAAAVQFRDDGKVETLSSAPDSQLESLLLQDLSAEPQRIVSYDSRCAFNYGPISLLVNN
CHHEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCEECCCCHHEEECC
MPVDDEERCGKLKDYLALMAEGAAERANGMIRDGRPDRLDQIRDTLAAIAANQRASQYDA
CCCCCHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RALLDAMRAQQEDLLAAMGVSEAQAAPLLAVACEAQAALDRLLGRQMRELELLSLRLTDA
HHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
GR
CC
>Mature Secondary Structure
MEKHRMLVIDDDPIAHQIMQMLFEAELRLTCKVTGEEGLSAVEEEQPEIVLLDIEMEGMD
CCCCCEEEECCCHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHHCCCCEEEEEEEECCCC
GYEACRRIRQLNLPSQPYIIFVSAHSDPVKRLAAYSVGGEDFVSKPLQPQELTRKVEQAL
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHH
RYRREMHELKSQAQGAMSVAMSAMTDSSALGICLQYFRRLFQAADPGQLMQITLDALGEF
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
GLAAAVQFRDDGKVETLSSAPDSQLESLLLQDLSAEPQRIVSYDSRCAFNYGPISLLVNN
CHHEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCEECCCCHHEEECC
MPVDDEERCGKLKDYLALMAEGAAERANGMIRDGRPDRLDQIRDTLAAIAANQRASQYDA
CCCCCHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RALLDAMRAQQEDLLAAMGVSEAQAAPLLAVACEAQAALDRLLGRQMRELELLSLRLTDA
HHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
GR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339426; 12000953 [H]