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The map label for this gene is truC [H]

Identifier: 34497801

GI number: 34497801

Start: 2530831

End: 2531580

Strand: Reverse

Name: truC [H]

Synonym: CV_2346

Alternate gene names: 34497801

Gene position: 2531580-2530831 (Counterclockwise)

Preceding gene: 34497807

Following gene: 34497800

Centisome position: 53.28

GC content: 65.47

Gene sequence:

>750_bases
ATGCTGCAAATACTGTATCGAGACGATCTGCTGATCGCCATCCACAAGCCGTCCAACCTGCTGGTGCACCGCACCGAGCT
GGACCGCCACGAAACCCGTTTCGCCATCCAGATGCTGCGCGACCAGATCGGCCGGCGCGTCTATCCGGTGCACCGGCTGG
ACAAGGGCACCTCGGGCGCATTGCTGTTCGCGCTGGACAAGGACGCCGGCCGCGAATTGTCCTTGCAGTTCGAACGCCAG
CAGGTGTACAAGCGCTACCTCGCCGTGGTCCGCGGCCACCCCGACGACGCGGGCCATATCGACCATCCGCTCAGCCGCCG
CCCGGACGACCTGGAATGGATAGGCGAGAAAGTGGAAACCGCGCCGCAGCCGGCCGTCACCGATTACCGGACGCTGGCGC
GCGTAGAGTTGCCCATCATGGTGGAACGCTATCCCACCAGCCGCTACAGCCTGCTGGAGCTGGAACCGCAGACCGGCCGC
AAGCACCAATTGCGCCGCCATCTGAAACACATCGCCCACCCCATCATCGGCGACTCCACCCATGGCAAGGGCCGCCACAA
CCGGATGTTCGCAGACCAGCTGGGCTGCCGGCGCATGCTGCTGGCCTGCGTGGAAATGCGGCTGACCCACCCCATTACCG
GCCAGCCCCTGCTGCTGAACTGCCCGCTGGAGAGCAACTTCTCCGCGCTGCTGGCGCGCCTGGGCTGGACCGACGCCGCG
CGCGATGCCGGAATCGAATTAACCATCTGA

Upstream 100 bases:

>100_bases
GACTGTATTCAATTGGATTGATCCAACACAAAACATAACGGCTATATCCAAAGCAGCGTGCAAGCCCTACAATTCCGCCC
TGCCCAAAAGCTTTGTATCC

Downstream 100 bases:

>100_bases
AAGACAACAACAATGCTCAGCTATCGCCACGCCTTTCATGCCGGCAACCACGCCGACGTGCTCAAGCACCTGATCGAGAT
CGAGCTGCTGAATTACCTGG

Product: pseudouridylate synthase

Products: NA

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MLQILYRDDLLIAIHKPSNLLVHRTELDRHETRFAIQMLRDQIGRRVYPVHRLDKGTSGALLFALDKDAGRELSLQFERQ
QVYKRYLAVVRGHPDDAGHIDHPLSRRPDDLEWIGEKVETAPQPAVTDYRTLARVELPIMVERYPTSRYSLLELEPQTGR
KHQLRRHLKHIAHPIIGDSTHGKGRHNRMFADQLGCRRMLLACVEMRLTHPITGQPLLLNCPLESNFSALLARLGWTDAA
RDAGIELTI

Sequences:

>Translated_249_residues
MLQILYRDDLLIAIHKPSNLLVHRTELDRHETRFAIQMLRDQIGRRVYPVHRLDKGTSGALLFALDKDAGRELSLQFERQ
QVYKRYLAVVRGHPDDAGHIDHPLSRRPDDLEWIGEKVETAPQPAVTDYRTLARVELPIMVERYPTSRYSLLELEPQTGR
KHQLRRHLKHIAHPIIGDSTHGKGRHNRMFADQLGCRRMLLACVEMRLTHPITGQPLLLNCPLESNFSALLARLGWTDAA
RDAGIELTI
>Mature_249_residues
MLQILYRDDLLIAIHKPSNLLVHRTELDRHETRFAIQMLRDQIGRRVYPVHRLDKGTSGALLFALDKDAGRELSLQFERQ
QVYKRYLAVVRGHPDDAGHIDHPLSRRPDDLEWIGEKVETAPQPAVTDYRTLARVELPIMVERYPTSRYSLLELEPQTGR
KHQLRRHLKHIAHPIIGDSTHGKGRHNRMFADQLGCRRMLLACVEMRLTHPITGQPLLLNCPLESNFSALLARLGWTDAA
RDAGIELTI

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs [H]

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family [H]

Homologues:

Organism=Homo sapiens, GI221316667, Length=198, Percent_Identity=32.8282828282828, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI17158025, Length=167, Percent_Identity=34.1317365269461, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI221316663, Length=216, Percent_Identity=31.0185185185185, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1789155, Length=236, Percent_Identity=50.4237288135593, Blast_Score=244, Evalue=5e-66,
Organism=Escherichia coli, GI1786244, Length=234, Percent_Identity=35.042735042735, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788946, Length=239, Percent_Identity=30.5439330543933, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787327, Length=236, Percent_Identity=30.0847457627119, Blast_Score=93, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320168, Length=197, Percent_Identity=27.4111675126904, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324506, Length=183, Percent_Identity=28.4153005464481, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321608, Length=189, Percent_Identity=28.042328042328, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921090, Length=185, Percent_Identity=28.6486486486486, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24583449, Length=178, Percent_Identity=26.9662921348315, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583447, Length=178, Percent_Identity=26.9662921348315, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583445, Length=178, Percent_Identity=26.9662921348315, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 28656; Mature: 28656

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS01129 PSI_RLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQILYRDDLLIAIHKPSNLLVHRTELDRHETRFAIQMLRDQIGRRVYPVHRLDKGTSGA
CEEEEEECCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHCCEECCHHHCCCCCCCE
LLFALDKDAGRELSLQFERQQVYKRYLAVVRGHPDDAGHIDHPLSRRPDDLEWIGEKVET
EEEEECCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
APQPAVTDYRTLARVELPIMVERYPTSRYSLLELEPQTGRKHQLRRHLKHIAHPIIGDST
CCCCCHHHHHHHHHEECCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCC
HGKGRHNRMFADQLGCRRMLLACVEMRLTHPITGQPLLLNCPLESNFSALLARLGWTDAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHCCCCHH
RDAGIELTI
HCCCCEEEC
>Mature Secondary Structure
MLQILYRDDLLIAIHKPSNLLVHRTELDRHETRFAIQMLRDQIGRRVYPVHRLDKGTSGA
CEEEEEECCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHCCEECCHHHCCCCCCCE
LLFALDKDAGRELSLQFERQQVYKRYLAVVRGHPDDAGHIDHPLSRRPDDLEWIGEKVET
EEEEECCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
APQPAVTDYRTLARVELPIMVERYPTSRYSLLELEPQTGRKHQLRRHLKHIAHPIIGDST
CCCCCHHHHHHHHHEECCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCC
HGKGRHNRMFADQLGCRRMLLACVEMRLTHPITGQPLLLNCPLESNFSALLARLGWTDAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHCCCCHH
RDAGIELTI
HCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]