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The map label for this gene is sseJ [H]

Identifier: 34497721

GI number: 34497721

Start: 2456084

End: 2457097

Strand: Direct

Name: sseJ [H]

Synonym: CV_2266

Alternate gene names: 34497721

Gene position: 2456084-2457097 (Clockwise)

Preceding gene: 34497720

Following gene: 34497725

Centisome position: 51.7

GC content: 61.74

Gene sequence:

>1014_bases
GTGGCGGAGAATGGCAGATCCGGCCTGTTCGCGCTGGCATTGAAGAACCGGGAAGTTCAGCCCAGCCCAGCCGGCGAAGG
CAAGACGCTGGACCACATCAAGCGCCTGGTGGTGATAGGCGACAGCCTGTCGGATACGGAGGGCCGCATGCGCAGCAAAA
CGCTGGGCATCATGCCTTCGTCCCGCCAGTACTACGAAGGCCGGTTCACAAATGGCTTCGCCTGGCCGGATTTCGCCGCC
TCGCGGGCATTCTTGGACAAGCCGCTGGTGAACTACGCGGAGGGCGGCGCGGTGGCGGGCAAGTATTCCAAGCTGAACCC
CACCTTCATGTTCATTTCCAATATGGGCCGGCAGATCAAGAAGCACGATTTCAAGCCGGGCGACATGGCGGTGTTGGAGC
TGGGCGCCAATGACTACATGACTTTCGGCAAGACCAATGTGGACAAGGTGATTCATGTCTACGAGAAGCAGATCGGCAAG
CTGGTGGCCAAGGGCGTGAAAAACATCCTGGTCGCCGGCGTCCCCGACCTGTCCAAAACCGTGTCCGCGCGCAGCCCCAA
GCAGAAACCCGGCCATGCCGACAAGCTGGCCGCGTTGTCCCGCCAGCATAACGAGCAATTGCGGCAATCGGTGGAAAAGC
TGCGGGAGCGCCATCGCGGCGAGGGCGTGGAAATCCGCTTTTTCGACATCGGCGGAAAACTGGACTACCTGATGTCGCTG
GCGGGCGAAGTGGACTACGACACCCGCTTCAACAAACACGAAGGCTATATCGACTTGCCGCGCGTGTTTGGCTTCGCCGG
CGACACCCGGCCGCTGGACACCTCGCATCGGCATGTCTTCCATGATGAAGTGCACCCGAGCCAGGAAGTGCACCAGATCC
TGGCCTCCCACATCACGGACTTTATCCGGGAGGAGTATGGCAGACCGGAGGAGGCTGCTGCCGCAACTGACAGCCGGTTG
GAGCGCGCGGGCGTTTACGCTGGCGCGTCGTCCGCCGAGCAGCCAGTCCGCTGA

Upstream 100 bases:

>100_bases
TCGCGCTGAGCGAGCCTTCCCGTTGGGAGGGCAGGTGAAACAGGTGAGGACGGAAACGCTTTCCCCGCCACAGGCGGGCG
GGCCGCAAGCGTGCGCATGC

Downstream 100 bases:

>100_bases
CCCCGCAGGACTTCCGCAGTCAGCCGATCCAGGTTCTGCTCATTGCCAGGAATGACGATCGGCGCCATGCGCTTTCCATT
CTTTGGGTGAAAGGGCTTGG

Product: SPI2 translocated effector

Products: NA

Alternate protein names: Salmonella-translocated effector J; STE J [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MAENGRSGLFALALKNREVQPSPAGEGKTLDHIKRLVVIGDSLSDTEGRMRSKTLGIMPSSRQYYEGRFTNGFAWPDFAA
SRAFLDKPLVNYAEGGAVAGKYSKLNPTFMFISNMGRQIKKHDFKPGDMAVLELGANDYMTFGKTNVDKVIHVYEKQIGK
LVAKGVKNILVAGVPDLSKTVSARSPKQKPGHADKLAALSRQHNEQLRQSVEKLRERHRGEGVEIRFFDIGGKLDYLMSL
AGEVDYDTRFNKHEGYIDLPRVFGFAGDTRPLDTSHRHVFHDEVHPSQEVHQILASHITDFIREEYGRPEEAAAATDSRL
ERAGVYAGASSAEQPVR

Sequences:

>Translated_337_residues
MAENGRSGLFALALKNREVQPSPAGEGKTLDHIKRLVVIGDSLSDTEGRMRSKTLGIMPSSRQYYEGRFTNGFAWPDFAA
SRAFLDKPLVNYAEGGAVAGKYSKLNPTFMFISNMGRQIKKHDFKPGDMAVLELGANDYMTFGKTNVDKVIHVYEKQIGK
LVAKGVKNILVAGVPDLSKTVSARSPKQKPGHADKLAALSRQHNEQLRQSVEKLRERHRGEGVEIRFFDIGGKLDYLMSL
AGEVDYDTRFNKHEGYIDLPRVFGFAGDTRPLDTSHRHVFHDEVHPSQEVHQILASHITDFIREEYGRPEEAAAATDSRL
ERAGVYAGASSAEQPVR
>Mature_336_residues
AENGRSGLFALALKNREVQPSPAGEGKTLDHIKRLVVIGDSLSDTEGRMRSKTLGIMPSSRQYYEGRFTNGFAWPDFAAS
RAFLDKPLVNYAEGGAVAGKYSKLNPTFMFISNMGRQIKKHDFKPGDMAVLELGANDYMTFGKTNVDKVIHVYEKQIGKL
VAKGVKNILVAGVPDLSKTVSARSPKQKPGHADKLAALSRQHNEQLRQSVEKLRERHRGEGVEIRFFDIGGKLDYLMSLA
GEVDYDTRFNKHEGYIDLPRVFGFAGDTRPLDTSHRHVFHDEVHPSQEVHQILASHITDFIREEYGRPEEAAAATDSRLE
RAGVYAGASSAEQPVR

Specific function: Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is required for endosomal tubulation and negatively regulates the formation of Salmonella-induced filaments (Sifs) in epithelial cells. H

COG id: COG3240

COG function: function code IR; Phospholipase/lecithinase/hemolysin

Gene ontology:

Cell location: Secreted. Host cytoplasm. Note=Secreted via type III secretion system 2 (SPI-2 TTSS), and delivered into the host cytoplasm. Localizes to the Salmonella-containing vacuole (SCV) at early time points following invasion and subsequently traffics away from t

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR010637 [H]

Pfam domain/function: PF00657 Lipase_GDSL; PF06767 Sif [H]

EC number: NA

Molecular weight: Translated: 37485; Mature: 37354

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENGRSGLFALALKNREVQPSPAGEGKTLDHIKRLVVIGDSLSDTEGRMRSKTLGIMPS
CCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCC
SRQYYEGRFTNGFAWPDFAASRAFLDKPLVNYAEGGAVAGKYSKLNPTFMFISNMGRQIK
CCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KHDFKPGDMAVLELGANDYMTFGKTNVDKVIHVYEKQIGKLVAKGVKNILVAGVPDLSKT
HCCCCCCCEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
VSARSPKQKPGHADKLAALSRQHNEQLRQSVEKLRERHRGEGVEIRFFDIGGKLDYLMSL
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHH
AGEVDYDTRFNKHEGYIDLPRVFGFAGDTRPLDTSHRHVFHDEVHPSQEVHQILASHITD
HCCCCCCCCCCCCCCCEEHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHH
FIREEYGRPEEAAAATDSRLERAGVYAGASSAEQPVR
HHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AENGRSGLFALALKNREVQPSPAGEGKTLDHIKRLVVIGDSLSDTEGRMRSKTLGIMPS
CCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCC
SRQYYEGRFTNGFAWPDFAASRAFLDKPLVNYAEGGAVAGKYSKLNPTFMFISNMGRQIK
CCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KHDFKPGDMAVLELGANDYMTFGKTNVDKVIHVYEKQIGKLVAKGVKNILVAGVPDLSKT
HCCCCCCCEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
VSARSPKQKPGHADKLAALSRQHNEQLRQSVEKLRERHRGEGVEIRFFDIGGKLDYLMSL
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHH
AGEVDYDTRFNKHEGYIDLPRVFGFAGDTRPLDTSHRHVFHDEVHPSQEVHQILASHITD
HCCCCCCCCCCCCCCCEEHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHH
FIREEYGRPEEAAAATDSRLERAGVYAGASSAEQPVR
HHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10861017; 11677609 [H]