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The map label for this gene is argD [H]

Identifier: 34497711

GI number: 34497711

Start: 2444709

End: 2445947

Strand: Direct

Name: argD [H]

Synonym: CV_2256

Alternate gene names: 34497711

Gene position: 2444709-2445947 (Clockwise)

Preceding gene: 34497710

Following gene: 34497712

Centisome position: 51.46

GC content: 54.72

Gene sequence:

>1239_bases
ATGTATCTTTATCAGTCTCGATTGAAAGACCAGATTATTCATTCGGTCAAGGATGTTTATGTGCATGCGGCAGGCGGCAC
GTATACAGATGCCATCTCCGGCATGTTCAATGTTCCCTTTGGATATTCATGCGAGCCCATCAAGCAACGCATTGCCCAAA
CTTTGAATAATCTGCCTTTTCATCCCAAGGATCATTTTTATTCGCAGGAATGGTTTGAAGCCTCGGCGATGTTGCTAGAT
AGGGCGATGATGAGTGACGGCGCCTTGCTCTACGTGAACAGCGGGTCCGAGGCGATTGAGTCCTCGATTGCGATGGCCTT
GGAGTATCACCAACGCAAAGGCAATGTCAAAAAGCGCAAGATAATATGCCGCAAACACTCCTATCACGGCGCCACCCTCG
GCGCGCATAGCGTGAGCGGCAGGAATGACTTCCAGGAGCTTAGAAACAGCGGCTACGACACGATACGCATACACCCACCG
TTTGCCGTCACCCATCAGGGCAGGCCGCAGGAAGCGCACAGCGTGAGCGATGTGGAGAACATTATTTTGCAAACGGGTCC
GGAGCATATCGCCGGCATCATCTTTGAGCCGGTTAATCATTTGAAAGGCATGCGAGAAAGCGGAGGAGAATATCTGGCAG
GAATCCGTGAGCTGTGCGACCGCTACGATATCGTGATGATCACTGATGAAATCGTAACAGGGGCTTCCCGCACCGGCTCA
TTTCTGAATACGCAGCGTTTCGGCGTCGTGCCCGACATCGTGGCGCTGGGCAAAGGCATCAGCGGCGGCTATTACCCCGC
TTCGGTAATGGCAACCACTGCCAAGATCGCCTCGGTGTTCGAAAAGCGCGGCGCCTGGATTCCCTATTCCTACTCCCACA
CCTATGCCAGCAACCCGGTATCGATCGCATCCATCCATGCCTCATTGCAGGTGCTGGAGCAGGTCGAGTCATCGGGGCAA
CTGAGACGACTGATTGCCAAGGCGGCGGAGTTGACCAGCGAGATGGCTTCGAACGAACGCATAGCCAGGGCTGAGGCAAG
CGGGCTGCTGATCGGGCTGACGCTGGATGAGCGTCTTGGCGCCGGAGCGGGAAAGAGGCTTGAGCAGCTGTGCTTCGAAC
GCAAGCTTATTATCCGCGGCGATGAGAACTGGGCATGCCTGGCTCCCGCCTACGTGATGTCGGAGGCGGCGTTGCAAGAA
GCGTTCGAGGTGCTGAATGAATCGATTAATCACTTGTAA

Upstream 100 bases:

>100_bases
ATTATCGGAGCCGGCGCGGTGGTGGCCCGCTCGGTGCCGGCATTCAGCAAGGTTTTAGGTGCTCGTTCCCAGGTTTATCC
TCTTGCTATGGAAGGTTGAC

Downstream 100 bases:

>100_bases
GTCCCCGGTGGATAGCCTGTCAGCACAGCTTTTCGGCATAGACGCCTGCCTGCGGGGACGGCGCTCAGCTCTGAGCGATA
GCGAGTATGCGCGTGCGCAG

Product: acetylornithine aminotransferase

Products: N-acetyl-L-glutamate 5-semialdehyde; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MYLYQSRLKDQIIHSVKDVYVHAAGGTYTDAISGMFNVPFGYSCEPIKQRIAQTLNNLPFHPKDHFYSQEWFEASAMLLD
RAMMSDGALLYVNSGSEAIESSIAMALEYHQRKGNVKKRKIICRKHSYHGATLGAHSVSGRNDFQELRNSGYDTIRIHPP
FAVTHQGRPQEAHSVSDVENIILQTGPEHIAGIIFEPVNHLKGMRESGGEYLAGIRELCDRYDIVMITDEIVTGASRTGS
FLNTQRFGVVPDIVALGKGISGGYYPASVMATTAKIASVFEKRGAWIPYSYSHTYASNPVSIASIHASLQVLEQVESSGQ
LRRLIAKAAELTSEMASNERIARAEASGLLIGLTLDERLGAGAGKRLEQLCFERKLIIRGDENWACLAPAYVMSEAALQE
AFEVLNESINHL

Sequences:

>Translated_412_residues
MYLYQSRLKDQIIHSVKDVYVHAAGGTYTDAISGMFNVPFGYSCEPIKQRIAQTLNNLPFHPKDHFYSQEWFEASAMLLD
RAMMSDGALLYVNSGSEAIESSIAMALEYHQRKGNVKKRKIICRKHSYHGATLGAHSVSGRNDFQELRNSGYDTIRIHPP
FAVTHQGRPQEAHSVSDVENIILQTGPEHIAGIIFEPVNHLKGMRESGGEYLAGIRELCDRYDIVMITDEIVTGASRTGS
FLNTQRFGVVPDIVALGKGISGGYYPASVMATTAKIASVFEKRGAWIPYSYSHTYASNPVSIASIHASLQVLEQVESSGQ
LRRLIAKAAELTSEMASNERIARAEASGLLIGLTLDERLGAGAGKRLEQLCFERKLIIRGDENWACLAPAYVMSEAALQE
AFEVLNESINHL
>Mature_412_residues
MYLYQSRLKDQIIHSVKDVYVHAAGGTYTDAISGMFNVPFGYSCEPIKQRIAQTLNNLPFHPKDHFYSQEWFEASAMLLD
RAMMSDGALLYVNSGSEAIESSIAMALEYHQRKGNVKKRKIICRKHSYHGATLGAHSVSGRNDFQELRNSGYDTIRIHPP
FAVTHQGRPQEAHSVSDVENIILQTGPEHIAGIIFEPVNHLKGMRESGGEYLAGIRELCDRYDIVMITDEIVTGASRTGS
FLNTQRFGVVPDIVALGKGISGGYYPASVMATTAKIASVFEKRGAWIPYSYSHTYASNPVSIASIHASLQVLEQVESSGQ
LRRLIAKAAELTSEMASNERIARAEASGLLIGLTLDERLGAGAGKRLEQLCFERKLIIRGDENWACLAPAYVMSEAALQE
AFEVLNESINHL

Specific function: Essential for glycerol catabolism [H]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=329, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI284507298, Length=326, Percent_Identity=28.5276073619632, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI226442705, Length=359, Percent_Identity=26.7409470752089, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI37574042, Length=277, Percent_Identity=28.5198555956679, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI226442709, Length=277, Percent_Identity=28.5198555956679, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI24119277, Length=348, Percent_Identity=24.1379310344828, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1789759, Length=389, Percent_Identity=25.1928020565553, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI145693181, Length=382, Percent_Identity=25.3926701570681, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1786991, Length=326, Percent_Identity=28.5276073619632, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1788044, Length=423, Percent_Identity=23.6406619385343, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789016, Length=254, Percent_Identity=29.1338582677165, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787560, Length=302, Percent_Identity=26.158940397351, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786349, Length=252, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25144271, Length=279, Percent_Identity=25.8064516129032, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI25144274, Length=178, Percent_Identity=28.6516853932584, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323470, Length=346, Percent_Identity=27.7456647398844, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6324386, Length=366, Percent_Identity=26.775956284153, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324432, Length=423, Percent_Identity=23.1678486997636, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21357415, Length=337, Percent_Identity=25.5192878338279, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356575, Length=349, Percent_Identity=25.214899713467, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281366494, Length=287, Percent_Identity=24.7386759581882, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24667139, Length=287, Percent_Identity=24.7386759581882, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24667143, Length=287, Percent_Identity=24.7386759581882, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.11

Molecular weight: Translated: 45470; Mature: 45470

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLYQSRLKDQIIHSVKDVYVHAAGGTYTDAISGMFNVPFGYSCEPIKQRIAQTLNNLPF
CCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC
HPKDHFYSQEWFEASAMLLDRAMMSDGALLYVNSGSEAIESSIAMALEYHQRKGNVKKRK
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHH
IICRKHSYHGATLGAHSVSGRNDFQELRNSGYDTIRIHPPFAVTHQGRPQEAHSVSDVEN
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCEEECCCCCCCHHHHHHHHHH
IILQTGPEHIAGIIFEPVNHLKGMRESGGEYLAGIRELCDRYDIVMITDEIVTGASRTGS
HHHHCCHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCC
FLNTQRFGVVPDIVALGKGISGGYYPASVMATTAKIASVFEKRGAWIPYSYSHTYASNPV
CCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SIASIHASLQVLEQVESSGQLRRLIAKAAELTSEMASNERIARAEASGLLIGLTLDERLG
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEEHHHHC
AGAGKRLEQLCFERKLIIRGDENWACLAPAYVMSEAALQEAFEVLNESINHL
CCCHHHHHHHHHHCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYLYQSRLKDQIIHSVKDVYVHAAGGTYTDAISGMFNVPFGYSCEPIKQRIAQTLNNLPF
CCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC
HPKDHFYSQEWFEASAMLLDRAMMSDGALLYVNSGSEAIESSIAMALEYHQRKGNVKKRK
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHH
IICRKHSYHGATLGAHSVSGRNDFQELRNSGYDTIRIHPPFAVTHQGRPQEAHSVSDVEN
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCEEECCCCCCCHHHHHHHHHH
IILQTGPEHIAGIIFEPVNHLKGMRESGGEYLAGIRELCDRYDIVMITDEIVTGASRTGS
HHHHCCHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCC
FLNTQRFGVVPDIVALGKGISGGYYPASVMATTAKIASVFEKRGAWIPYSYSHTYASNPV
CCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SIASIHASLQVLEQVESSGQLRRLIAKAAELTSEMASNERIARAEASGLLIGLTLDERLG
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEEHHHHC
AGAGKRLEQLCFERKLIIRGDENWACLAPAYVMSEAALQEAFEVLNESINHL
CCCHHHHHHHHHHCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5-phosphate [C]

Metal ions: Ni2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.1 {N2-acetyl-L-ornithine}} 1.54 {N2-acetyl-L-ornithine}} 1.3 {N2-acetyl-L-ornithine}} 1.1 {N2-acetyl-L-ornithine}} 0.48 {N2-acetyl-L-ornithine}} 0.34 {N2-acetyl-L-ornithine}} 2.5 {2-oxoglutarate}} [C]

Substrates: N2-acetyl-L-ornithine; 2-oxoglutarate

Specific reaction: N2-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate

General reaction: Amino group transfer [C]

Inhibitor: Cu2+; Ornithine; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8969498; 8436953 [H]