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The map label for this gene is yafV [C]

Identifier: 34497552

GI number: 34497552

Start: 2287879

End: 2288688

Strand: Direct

Name: yafV [C]

Synonym: CV_2097

Alternate gene names: 34497552

Gene position: 2287879-2288688 (Clockwise)

Preceding gene: 34497551

Following gene: 34497553

Centisome position: 48.15

GC content: 66.79

Gene sequence:

>810_bases
ATGAAGCAGAAGTTTGTCGCCGCAGCCGTGCAGATGGTTTCCGGTTGCGGCCTGGAGGCCAACCTGGCCCGCGCCGACCA
GCTGCTGGGAGAGGCCGCCGCGCGCGGCGCCGGCCTGGCGGTGTTGCCGGAGTATTTTTGCCTGATGGGCGCGAAGGAAA
CCGACAAGGTAGCCATCCGCGAACCGTTCGGCGGCGGCCCCATCCAGCAGGCGCTGTCGGAGATGGCGCGCCGCCATGGC
CTGTGGCTGCTGGGCGGCACCGTGCCGCTTGTCTGCGAGGAAGAGGGCAAGGTGTTCAACAGCAGCCTGCTCTACAATCC
GCAGGGAGAGGTGGCGGCCCGCTACGACAAGATCCACCTGTTCGGTTTCACCGGCCAGGGAGAAAGTTATTGCGAGTCCA
ACACCATACGCCCGGGCGTGACGCCGACCAAGGCGGAAACGCCGCTGGGCGACATCGCTTTCGGCATCTGCTACGACCTG
CGCTTCCCGGAGCTGTTCCGCATGCTGGCGCCGGTCGACCTGCTGATCTTGCCGGCGGCCTTCACCGCCACCACCGGCGA
AGCGCACTGGGAGCCGCTGCTGCGGGCCCGGGCAATAGAAAACCAGTGTTACCTGATTGCCTCCGCGCAGGGCGGAAGGC
ATGAAAACGGCCGGCAGACGCACGGCCACTCGATGATCATCGACCCGTGGGGGCGCATTCTCGCCGAACTGCCGAGCGGC
GAGGGCGTCATCACCGCCGAGATCGATCCCGAATGGACGGATTCGGTGCGGAGCCGCCTGCCGGCTCTGGCGCACCGGGT
CATACGCTGA

Upstream 100 bases:

>100_bases
AGATATTGTCGCTTGAGTACGAGGTGAGCGGTTCGTTGAAGGATCCGCAGGTGAAGCGGGTGAACGTCGGCGATCCGGTC
CAGATCAAGGGGAAGAAGCC

Downstream 100 bases:

>100_bases
AAGAGAATATGCAAGACGCCTTCACCATCGCAGACGAACTGCTGCTCAAGCCCTACGGCCTGGACGAGGCCGCGCTGGAG
CGCACGCTGTCGCATATGCG

Product: nitrilase

Products: carboxylate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKQKFVAAAVQMVSGCGLEANLARADQLLGEAAARGAGLAVLPEYFCLMGAKETDKVAIREPFGGGPIQQALSEMARRHG
LWLLGGTVPLVCEEEGKVFNSSLLYNPQGEVAARYDKIHLFGFTGQGESYCESNTIRPGVTPTKAETPLGDIAFGICYDL
RFPELFRMLAPVDLLILPAAFTATTGEAHWEPLLRARAIENQCYLIASAQGGRHENGRQTHGHSMIIDPWGRILAELPSG
EGVITAEIDPEWTDSVRSRLPALAHRVIR

Sequences:

>Translated_269_residues
MKQKFVAAAVQMVSGCGLEANLARADQLLGEAAARGAGLAVLPEYFCLMGAKETDKVAIREPFGGGPIQQALSEMARRHG
LWLLGGTVPLVCEEEGKVFNSSLLYNPQGEVAARYDKIHLFGFTGQGESYCESNTIRPGVTPTKAETPLGDIAFGICYDL
RFPELFRMLAPVDLLILPAAFTATTGEAHWEPLLRARAIENQCYLIASAQGGRHENGRQTHGHSMIIDPWGRILAELPSG
EGVITAEIDPEWTDSVRSRLPALAHRVIR
>Mature_269_residues
MKQKFVAAAVQMVSGCGLEANLARADQLLGEAAARGAGLAVLPEYFCLMGAKETDKVAIREPFGGGPIQQALSEMARRHG
LWLLGGTVPLVCEEEGKVFNSSLLYNPQGEVAARYDKIHLFGFTGQGESYCESNTIRPGVTPTKAETPLGDIAFGICYDL
RFPELFRMLAPVDLLILPAAFTATTGEAHWEPLLRARAIENQCYLIASAQGGRHENGRQTHGHSMIIDPWGRILAELPSG
EGVITAEIDPEWTDSVRSRLPALAHRVIR

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632350, Length=269, Percent_Identity=41.2639405204461, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI297632348, Length=269, Percent_Identity=41.2639405204461, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI5031947, Length=269, Percent_Identity=41.2639405204461, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI9910460, Length=267, Percent_Identity=35.9550561797753, Blast_Score=166, Evalue=1e-41,
Organism=Homo sapiens, GI297632346, Length=196, Percent_Identity=38.265306122449, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1786412, Length=244, Percent_Identity=31.5573770491803, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556280, Length=264, Percent_Identity=36.7424242424242, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6323383, Length=277, Percent_Identity=35.0180505415162, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322335, Length=299, Percent_Identity=32.4414715719064, Blast_Score=142, Evalue=6e-35,
Organism=Drosophila melanogaster, GI17933642, Length=270, Percent_Identity=40, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI21355835, Length=232, Percent_Identity=33.1896551724138, Blast_Score=142, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.5.1

Molecular weight: Translated: 29196; Mature: 29196

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKFVAAAVQMVSGCGLEANLARADQLLGEAAARGAGLAVLPEYFCLMGAKETDKVAIR
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEE
EPFGGGPIQQALSEMARRHGLWLLGGTVPLVCEEEGKVFNSSLLYNPQGEVAARYDKIHL
CCCCCCHHHHHHHHHHHHCCEEEECCCEEEEECCCCCEECCEEEECCCCCEEEEEEEEEE
FGFTGQGESYCESNTIRPGVTPTKAETPLGDIAFGICYDLRFPELFRMLAPVDLLILPAA
EEECCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCHHHEEECCH
FTATTGEAHWEPLLRARAIENQCYLIASAQGGRHENGRQTHGHSMIIDPWGRILAELPSG
HCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHCCCC
EGVITAEIDPEWTDSVRSRLPALAHRVIR
CEEEEEECCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQKFVAAAVQMVSGCGLEANLARADQLLGEAAARGAGLAVLPEYFCLMGAKETDKVAIR
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEE
EPFGGGPIQQALSEMARRHGLWLLGGTVPLVCEEEGKVFNSSLLYNPQGEVAARYDKIHL
CCCCCCHHHHHHHHHHHHCCEEEECCCEEEEECCCCCEECCEEEECCCCCEEEEEEEEEE
FGFTGQGESYCESNTIRPGVTPTKAETPLGDIAFGICYDLRFPELFRMLAPVDLLILPAA
EEECCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCHHHEEECCH
FTATTGEAHWEPLLRARAIENQCYLIASAQGGRHENGRQTHGHSMIIDPWGRILAELPSG
HCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHCCCC
EGVITAEIDPEWTDSVRSRLPALAHRVIR
CEEEEEECCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrile; H2O

Specific reaction: A nitrile + H2O = a carboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]