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The map label for this gene is rfaF [H]

Identifier: 34497547

GI number: 34497547

Start: 2278770

End: 2279807

Strand: Reverse

Name: rfaF [H]

Synonym: CV_2092

Alternate gene names: 34497547

Gene position: 2279807-2278770 (Counterclockwise)

Preceding gene: 34497548

Following gene: 34497546

Centisome position: 47.99

GC content: 67.82

Gene sequence:

>1038_bases
ATGAAGAAGAAAATCCTGGTCATCGGCCCCTCCTGGGTCGGTGACAGCGTGATGGCCCAGCCGCTGTACCGCCGCCTGCA
CCAGCGCCACCCCGGACTGGAACTGCACGTGTTCGCGCCGGCGTGGACCCTGCCGCTGCTGGCCCGGATGCCGGAAGTGG
CCCAGTCCCACCTCAACCCCTTCGGCCACGGCGCGCTGCGCCTGGTCGAGCGCTGGAAAGTGGCCCGCCACCTGGCGCGC
GAACGTTTCGACCAGGTGATCGTGCTGCCCAACTCGCTGAAGTCGGCGCTGATTCCGCTGTTCGCCCGCATTCCGCTGCG
CACCGGCTTTCTCGGCGAATCCCGCTATGGCCTGCTCAACGATGCCCGCGAGCTGGACGAGCAGGAACTGCCGATGATGG
TGGAGCGCTTCTGCGCGCTGGCGGAAGACCGCAAGCAGCCGCTGCCGCGCCCCATTCCCAACCCGCAGCTGATCGCCACC
CCGGCGGTCCAGCGACAGGCTGCGGCCAAGCTGGGCCTGGATGTCTCGCGCCCCATCGTCGCCTTCTGCCCCGGCGCGGA
ATACGGCCCGGCCAAGCGCTGGCCCGCCCGCCACTTCGCCGAGCTGGCCAAGCGCTTCCAGGCCGCCGGCTATGCGGTGT
GGCTGTTCGGCTCGCCCAAGGACAAGCAGATCGGCGACGACATCGCTCGGCTGGCCGGCGACGCCGCCGTCAATCTGTGC
GGCGCCACCGGGCTGGACGAAGCGATAGACCTGATGGGCCTGGCCAAGCTGGCCGTATGCAACGATTCGGGACTGATGCA
CGTCGCCGCCGCGCTCGACAAACCGTTGGTGGCCCTGTACGGCTCCTCCAGCCCCGACTTCACTCCGCCGCTGTCGCAAC
GGGCAGAGATCGTGTCGCTGAATCTGGATTGCAGCCCCTGTTTCGAGCGCAGCTGCCCGTACGGCCACACCGACTGCCTG
GAAAAAATGCAGCCGGAGTTAGTGTGGCAGGCCGCGCAACGACTACTCCCCTCGCTTGTCCCCATCGCACAGGATTGA

Upstream 100 bases:

>100_bases
ACGGCAAAGGGTGTGGCGCGGGCCACACCTTTTTTGATTCCCGCAGCCAAACCCAAGCCGCGCTGGCCAGCCGGCTTGGG
TTTGGAACCGGAAATACCCA

Downstream 100 bases:

>100_bases
CATGAGCCCAGTGGAACCCACCCGCCCCCCCGAGGAAAATGGCCGGCGCATGGAGCTGGTCCGCGCCGCCGCCATCCTGT
TCCGCGACCAGGGCTACGAA

Product: ADP-heptose--LPS heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKKKILVIGPSWVGDSVMAQPLYRRLHQRHPGLELHVFAPAWTLPLLARMPEVAQSHLNPFGHGALRLVERWKVARHLAR
ERFDQVIVLPNSLKSALIPLFARIPLRTGFLGESRYGLLNDARELDEQELPMMVERFCALAEDRKQPLPRPIPNPQLIAT
PAVQRQAAAKLGLDVSRPIVAFCPGAEYGPAKRWPARHFAELAKRFQAAGYAVWLFGSPKDKQIGDDIARLAGDAAVNLC
GATGLDEAIDLMGLAKLAVCNDSGLMHVAAALDKPLVALYGSSSPDFTPPLSQRAEIVSLNLDCSPCFERSCPYGHTDCL
EKMQPELVWQAAQRLLPSLVPIAQD

Sequences:

>Translated_345_residues
MKKKILVIGPSWVGDSVMAQPLYRRLHQRHPGLELHVFAPAWTLPLLARMPEVAQSHLNPFGHGALRLVERWKVARHLAR
ERFDQVIVLPNSLKSALIPLFARIPLRTGFLGESRYGLLNDARELDEQELPMMVERFCALAEDRKQPLPRPIPNPQLIAT
PAVQRQAAAKLGLDVSRPIVAFCPGAEYGPAKRWPARHFAELAKRFQAAGYAVWLFGSPKDKQIGDDIARLAGDAAVNLC
GATGLDEAIDLMGLAKLAVCNDSGLMHVAAALDKPLVALYGSSSPDFTPPLSQRAEIVSLNLDCSPCFERSCPYGHTDCL
EKMQPELVWQAAQRLLPSLVPIAQD
>Mature_345_residues
MKKKILVIGPSWVGDSVMAQPLYRRLHQRHPGLELHVFAPAWTLPLLARMPEVAQSHLNPFGHGALRLVERWKVARHLAR
ERFDQVIVLPNSLKSALIPLFARIPLRTGFLGESRYGLLNDARELDEQELPMMVERFCALAEDRKQPLPRPIPNPQLIAT
PAVQRQAAAKLGLDVSRPIVAFCPGAEYGPAKRWPARHFAELAKRFQAAGYAVWLFGSPKDKQIGDDIARLAGDAAVNLC
GATGLDEAIDLMGLAKLAVCNDSGLMHVAAALDKPLVALYGSSSPDFTPPLSQRAEIVSLNLDCSPCFERSCPYGHTDCL
EKMQPELVWQAAQRLLPSLVPIAQD

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=342, Percent_Identity=50.2923976608187, Blast_Score=317, Evalue=6e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 38028; Mature: 38028

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKILVIGPSWVGDSVMAQPLYRRLHQRHPGLELHVFAPAWTLPLLARMPEVAQSHLNP
CCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCHHHHHHHCCC
FGHGALRLVERWKVARHLARERFDQVIVLPNSLKSALIPLFARIPLRTGFLGESRYGLLN
CCHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHHCCHHCCCCCCCCCCHHH
DARELDEQELPMMVERFCALAEDRKQPLPRPIPNPQLIATPAVQRQAAAKLGLDVSRPIV
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCCCEE
AFCPGAEYGPAKRWPARHFAELAKRFQAAGYAVWLFGSPKDKQIGDDIARLAGDAAVNLC
EECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GATGLDEAIDLMGLAKLAVCNDSGLMHVAAALDKPLVALYGSSSPDFTPPLSQRAEIVSL
CCCCHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHCCCEEEEE
NLDCSPCFERSCPYGHTDCLEKMQPELVWQAAQRLLPSLVPIAQD
ECCCCHHHHCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKKILVIGPSWVGDSVMAQPLYRRLHQRHPGLELHVFAPAWTLPLLARMPEVAQSHLNP
CCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCHHHHHHHCCC
FGHGALRLVERWKVARHLARERFDQVIVLPNSLKSALIPLFARIPLRTGFLGESRYGLLN
CCHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHHCCHHCCCCCCCCCCHHH
DARELDEQELPMMVERFCALAEDRKQPLPRPIPNPQLIATPAVQRQAAAKLGLDVSRPIV
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCCCEE
AFCPGAEYGPAKRWPARHFAELAKRFQAAGYAVWLFGSPKDKQIGDDIARLAGDAAVNLC
EECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GATGLDEAIDLMGLAKLAVCNDSGLMHVAAALDKPLVALYGSSSPDFTPPLSQRAEIVSL
CCCCHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHCCCEEEEE
NLDCSPCFERSCPYGHTDCLEKMQPELVWQAAQRLLPSLVPIAQD
ECCCCHHHHCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]