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The map label for this gene is lpdA [H]

Identifier: 34497492

GI number: 34497492

Start: 2219810

End: 2221204

Strand: Direct

Name: lpdA [H]

Synonym: CV_2037

Alternate gene names: 34497492

Gene position: 2219810-2221204 (Clockwise)

Preceding gene: 34497491

Following gene: 34497493

Centisome position: 46.72

GC content: 69.18

Gene sequence:

>1395_bases
ATGAAAACCATCCACATCGACGTAGCCGTCATCGGCGCCGGCACCGCCGGCCTCGCCGCCTACCGCGCCGCCAAGGCCGG
CGGCGCCTCCGCCCTGATCATCGAGGGCGGTCCTTACGGCACCACCTGCGCCCGCGTCGGCTGCATGCCGTCCAAGCTGT
TGATCGCCGCCGCCGAGGCCGCCCATCACGCCGGCCACACTGACATGTTCGGCGTGCATGTGGATGGCGGGGTCCGCATC
GACGGCCGCGAGGTGATGGCCCGGGTGCGCAGCGAGCGCGACCGCTTCGTCGGCTTCGTGGTGCGCAGCGTGGACGGCAT
TCCGGCCGAAGACAAGCTGCCCGGCTATGCCCGTTTCGTCGACAACACCACGCTGCAGGTCGATGACCACACCATCGTCC
AGGCCAAGCGGGTGGTGATCGCCACCGGCTCGTCGCCGGCCGTCCCGGCGCCGTTCAAGGCGTTCGGCGACCGTTTGATC
GTCAATGACGACGTATTCGAATGGGATGCGCTGCCGCAAAGCGTGGCGGTGTTCGGCCCCGGCGTGATCGGCCTGGAGCT
GGGCCAGGCGCTGTCCCGGCTGGGCGTGCGGGTACGCGTGTTCGGCTTGGGCGGCGGCGTGGGCCCGCTGTCCGATCCGG
CGGTGCGCGGCTACGCCCGCCAGGCCTTGTCCGAGCAGTTCTATCTGGATCCGGACGCCAAGGTGCTGGAGATGGCCAAT
GACGGCGATGCCGCGCGCATCCGCTACGTGAACCTGGACGGAGAAGAGGTCGAGGAGCGCTTCGATTACGTGCTGGCCGC
CACCGGCCGCACGCCGAACGTCCGCAGCCTGGGGCTGGAGAATACCAGCGTGCGGCTCGATGCCCGCGGCGTGCCGCTGT
TCGACCCGGAAACGCTGCTGTGCGTCGGCGCGCCGGTGTTCATCGCCGGCGACGCCAACAACATCCTGCCGCTGCTGCAC
GAAGCGGCCGACGAGGGCAAGACCGCCGGCGCCAACGCCGCCGCCTATCCGGCGGTATCGGCTGGACTGCGCCGCTCGCC
CATCGCGGTGGTGTTCTCTGACCCGCAGATGATGATGGTGGGACGCCGCTTCGCCGATCTGCCGGAGGGGGACTTCGCGG
TTGGGGAAGTCAGCTTCGAGGATCAGGGCCGCAGCCGGGTGATGGGCGTCAACAAGGGCCTGCTGCATGTGTATGCGGAC
AAGGCGACCGGCCGTTTCCTGGGCGCCGAGATGATAGGCCCGCGTGCCGAGAACCTGGCCCATCTGCTGGCCTGGAGCCA
TCAGCAGGGCTTGACCGTAGAGCAGATGCTGGACATGCCGTTCTACCACCCGGTGATAGAAGAGGGCCTGCGCACCGCGC
TGCGCGACGCGGCGGCGAAGCTGGCGCGGGACTGA

Upstream 100 bases:

>100_bases
GTACTCCTGCCGGCCGTTTTTTCCAGCCGGGATGCGGGACGGCATGCCCATTGCGCGCTGCGTGCCGCGCCGCATCCCCG
CCAGATAGAGGATAAGAACC

Downstream 100 bases:

>100_bases
GCCGGGCGGGCGTCCGCCTGTTTGACGCAAGCCCCCGGCCCGCGATGGTTTTTGCCATCGCGGGCTTTTCTCATGGCGCA
AGGCGCGGTTCGAAGCCTTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKTIHIDVAVIGAGTAGLAAYRAAKAGGASALIIEGGPYGTTCARVGCMPSKLLIAAAEAAHHAGHTDMFGVHVDGGVRI
DGREVMARVRSERDRFVGFVVRSVDGIPAEDKLPGYARFVDNTTLQVDDHTIVQAKRVVIATGSSPAVPAPFKAFGDRLI
VNDDVFEWDALPQSVAVFGPGVIGLELGQALSRLGVRVRVFGLGGGVGPLSDPAVRGYARQALSEQFYLDPDAKVLEMAN
DGDAARIRYVNLDGEEVEERFDYVLAATGRTPNVRSLGLENTSVRLDARGVPLFDPETLLCVGAPVFIAGDANNILPLLH
EAADEGKTAGANAAAYPAVSAGLRRSPIAVVFSDPQMMMVGRRFADLPEGDFAVGEVSFEDQGRSRVMGVNKGLLHVYAD
KATGRFLGAEMIGPRAENLAHLLAWSHQQGLTVEQMLDMPFYHPVIEEGLRTALRDAAAKLARD

Sequences:

>Translated_464_residues
MKTIHIDVAVIGAGTAGLAAYRAAKAGGASALIIEGGPYGTTCARVGCMPSKLLIAAAEAAHHAGHTDMFGVHVDGGVRI
DGREVMARVRSERDRFVGFVVRSVDGIPAEDKLPGYARFVDNTTLQVDDHTIVQAKRVVIATGSSPAVPAPFKAFGDRLI
VNDDVFEWDALPQSVAVFGPGVIGLELGQALSRLGVRVRVFGLGGGVGPLSDPAVRGYARQALSEQFYLDPDAKVLEMAN
DGDAARIRYVNLDGEEVEERFDYVLAATGRTPNVRSLGLENTSVRLDARGVPLFDPETLLCVGAPVFIAGDANNILPLLH
EAADEGKTAGANAAAYPAVSAGLRRSPIAVVFSDPQMMMVGRRFADLPEGDFAVGEVSFEDQGRSRVMGVNKGLLHVYAD
KATGRFLGAEMIGPRAENLAHLLAWSHQQGLTVEQMLDMPFYHPVIEEGLRTALRDAAAKLARD
>Mature_464_residues
MKTIHIDVAVIGAGTAGLAAYRAAKAGGASALIIEGGPYGTTCARVGCMPSKLLIAAAEAAHHAGHTDMFGVHVDGGVRI
DGREVMARVRSERDRFVGFVVRSVDGIPAEDKLPGYARFVDNTTLQVDDHTIVQAKRVVIATGSSPAVPAPFKAFGDRLI
VNDDVFEWDALPQSVAVFGPGVIGLELGQALSRLGVRVRVFGLGGGVGPLSDPAVRGYARQALSEQFYLDPDAKVLEMAN
DGDAARIRYVNLDGEEVEERFDYVLAATGRTPNVRSLGLENTSVRLDARGVPLFDPETLLCVGAPVFIAGDANNILPLLH
EAADEGKTAGANAAAYPAVSAGLRRSPIAVVFSDPQMMMVGRRFADLPEGDFAVGEVSFEDQGRSRVMGVNKGLLHVYAD
KATGRFLGAEMIGPRAENLAHLLAWSHQQGLTVEQMLDMPFYHPVIEEGLRTALRDAAAKLARD

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=468, Percent_Identity=27.7777777777778, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI50301238, Length=311, Percent_Identity=25.4019292604502, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1786307, Length=472, Percent_Identity=25.8474576271186, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87082354, Length=476, Percent_Identity=25.6302521008403, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87081717, Length=458, Percent_Identity=24.0174672489083, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789915, Length=282, Percent_Identity=30.1418439716312, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32565766, Length=459, Percent_Identity=27.8867102396514, Blast_Score=141, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17557007, Length=301, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71983429, Length=282, Percent_Identity=25.531914893617, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71983419, Length=282, Percent_Identity=25.531914893617, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=26.3157894736842, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6325166, Length=299, Percent_Identity=24.4147157190635, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6325240, Length=480, Percent_Identity=23.75, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21358499, Length=465, Percent_Identity=29.4623655913978, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24640553, Length=318, Percent_Identity=29.2452830188679, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640549, Length=318, Percent_Identity=29.2452830188679, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640551, Length=319, Percent_Identity=29.7805642633229, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17737741, Length=318, Percent_Identity=28.9308176100629, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49289; Mature: 49289

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIHIDVAVIGAGTAGLAAYRAAKAGGASALIIEGGPYGTTCARVGCMPSKLLIAAAEA
CEEEEEEEEEEECCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCHHHHHHHHHH
AHHAGHTDMFGVHVDGGVRIDGREVMARVRSERDRFVGFVVRSVDGIPAEDKLPGYARFV
HHCCCCCCEEEEEECCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
DNTTLQVDDHTIVQAKRVVIATGSSPAVPAPFKAFGDRLIVNDDVFEWDALPQSVAVFGP
CCCEEEECCCEEEEEEEEEEECCCCCCCCCCHHHHCCEEEECCCCEECCCCCCCEEHCCC
GVIGLELGQALSRLGVRVRVFGLGGGVGPLSDPAVRGYARQALSEQFYLDPDAKVLEMAN
CCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCEEECCCCCEEEECC
DGDAARIRYVNLDGEEVEERFDYVLAATGRTPNVRSLGLENTSVRLDARGVPLFDPETLL
CCCCEEEEEEECCHHHHHHHHCEEEEECCCCCCCCEECCCCCEEEEECCCCCCCCCCEEE
CVGAPVFIAGDANNILPLLHEAADEGKTAGANAAAYPAVSAGLRRSPIAVVFSDPQMMMV
EECCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCEEEEECCCCEEEH
GRRFADLPEGDFAVGEVSFEDQGRSRVMGVNKGLLHVYADKATGRFLGAEMIGPRAENLA
HHHHHCCCCCCEEEEEEEECCCCCCEEEECCCCEEEEEECCCCCCEECHHHCCCCHHHHH
HLLAWSHQQGLTVEQMLDMPFYHPVIEEGLRTALRDAAAKLARD
HHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTIHIDVAVIGAGTAGLAAYRAAKAGGASALIIEGGPYGTTCARVGCMPSKLLIAAAEA
CEEEEEEEEEEECCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCHHHHHHHHHH
AHHAGHTDMFGVHVDGGVRIDGREVMARVRSERDRFVGFVVRSVDGIPAEDKLPGYARFV
HHCCCCCCEEEEEECCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
DNTTLQVDDHTIVQAKRVVIATGSSPAVPAPFKAFGDRLIVNDDVFEWDALPQSVAVFGP
CCCEEEECCCEEEEEEEEEEECCCCCCCCCCHHHHCCEEEECCCCEECCCCCCCEEHCCC
GVIGLELGQALSRLGVRVRVFGLGGGVGPLSDPAVRGYARQALSEQFYLDPDAKVLEMAN
CCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCEEECCCCCEEEECC
DGDAARIRYVNLDGEEVEERFDYVLAATGRTPNVRSLGLENTSVRLDARGVPLFDPETLL
CCCCEEEEEEECCHHHHHHHHCEEEEECCCCCCCCEECCCCCEEEEECCCCCCCCCCEEE
CVGAPVFIAGDANNILPLLHEAADEGKTAGANAAAYPAVSAGLRRSPIAVVFSDPQMMMV
EECCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCEEEEECCCCEEEH
GRRFADLPEGDFAVGEVSFEDQGRSRVMGVNKGLLHVYADKATGRFLGAEMIGPRAENLA
HHHHHCCCCCCEEEEEEEECCCCCCEEEECCCCEEEEEECCCCCCEECHHHCCCCHHHHH
HLLAWSHQQGLTVEQMLDMPFYHPVIEEGLRTALRDAAAKLARD
HHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]