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The map label for this gene is puuA [H]

Identifier: 34497479

GI number: 34497479

Start: 2205176

End: 2206510

Strand: Direct

Name: puuA [H]

Synonym: CV_2024

Alternate gene names: 34497479

Gene position: 2205176-2206510 (Clockwise)

Preceding gene: 34497478

Following gene: 34497480

Centisome position: 46.41

GC content: 63.22

Gene sequence:

>1335_bases
ATGAATCACCAGATCAACGAGTGGTTGCGAGAGCACCGCATCACCGAAGTCGAATGCATCGTGCCGGACATGACCGGCAT
CGCCCGCGGAAAGATCGTGCCCAAGGACAAGTTCGTTTCCGACCCGGAAATGCGTCTGCCCGAGGCGGTGCTGATCCAGA
CCGTGACCGGCGACTATCCCGACGACAGCCTGCTGGATCTGACCGATCCGGACATGGTGCTGGAGCCCGATCCGAATACC
TTGCGCTACGTGCCGTGGGCGGTGGACCCGACCGCGCAATTGATTTACGACTGCTGCCGCGCCGACGGCAGCCCGGTGGA
CGTGGCGCCGCGCAATGTGCTCAAGCGCGTGCTGGGCCTGTTCGACGAGATGGGTCTGGAGCCGGTGCTGGCGCCGGAGA
TGGAGTTCTACCTGCTGTCGCCCAACCCCGACCCGGACATCCCGCTGGCCGCGCCGATCGGCCGCACCGGTCGGGCGGAA
TTCGGCCGTCGTTCCTACTCGATCGACGCAGTCAACGAGTTCGACCCGCTGTTCGAGGACATCTACGACTATTGCCACGC
GCAGAACCTGGAAGTGGACACGCTGATCCACGAGATCGGCACTGCGCAGATGGAAATCAACTTCCTGCATGGCAATCCGC
TGGAGCTGGCCGACCAGGTGTTCCTGTTCAAGCGCACCGTGCGCGAGGCGGCGTTCCGCCACAATATGTACGCCACTTTC
ATGGCCAAGCCGATGGAGAACGAGCCGGGCAGCGCCATGCACATCCACCAGAGCCTGGTGGACAAGAACACCGGCCGCAA
CGTGTTCACCAACGAGGACGGCAGCCCGTCCGACATCTTTTTCCATTTCATCGCCGGCCTGCAGAAATACGTGCCGCAGA
CCATGCCGCTGTTCGCCCCCTACGTGAACTCATTCCGTCGTTTGTCTCGCTACACCGCGGCGCCGACCAATGTCGAATGG
GGTTACGACAACCGCACCGTCGGGCTGCGCATCCCGCATTCGTCGCCGGCCGCGCGCCGCATCGAAAACCGGGTGCCCGG
CGTCGACGTCAATCCCTATATCGCGATCGCGGCCACGCTGGCCTGCGGCTACCTGGGCATGGTGAACAAGCTGCGGCCGA
CCGAGCCCAGGCAGACCGACGCTTACGAGCTGCCCTACCAATTCCCCAACAGCTCGGAAGAGTCGCTGCTGCAGCTGGCC
GGCTGCAGCGAGATCGCCGAGGTGTTGGGGCCGCGCTTCGTCAAGATGTACGTGGCGATGAAGGAGAAGGAGTTTTCCGA
GTATTTCCGCGTGATCAGCCCGTGGGAGCGCAAGTTCCTGCTGCTGCACGTGTGA

Upstream 100 bases:

>100_bases
GCCGGACGAGGAACCCGGCGCGAGTCTTGATGACTCATAAAGTAGCACCTCCCGATCCAGAGCCGCAGCCAGCGGCCGGG
GAGACCATAGAGGAAGCGTT

Downstream 100 bases:

>100_bases
TGCGCGGACCATCCGCTCGCGGTTAGAATCGCGGAACAGACAGGACAAACGAATAGAAAGACGCCGCAGGCGCAGGAGGG
GCGGGCAAGGGCCCGCCTCG

Product: glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MNHQINEWLREHRITEVECIVPDMTGIARGKIVPKDKFVSDPEMRLPEAVLIQTVTGDYPDDSLLDLTDPDMVLEPDPNT
LRYVPWAVDPTAQLIYDCCRADGSPVDVAPRNVLKRVLGLFDEMGLEPVLAPEMEFYLLSPNPDPDIPLAAPIGRTGRAE
FGRRSYSIDAVNEFDPLFEDIYDYCHAQNLEVDTLIHEIGTAQMEINFLHGNPLELADQVFLFKRTVREAAFRHNMYATF
MAKPMENEPGSAMHIHQSLVDKNTGRNVFTNEDGSPSDIFFHFIAGLQKYVPQTMPLFAPYVNSFRRLSRYTAAPTNVEW
GYDNRTVGLRIPHSSPAARRIENRVPGVDVNPYIAIAATLACGYLGMVNKLRPTEPRQTDAYELPYQFPNSSEESLLQLA
GCSEIAEVLGPRFVKMYVAMKEKEFSEYFRVISPWERKFLLLHV

Sequences:

>Translated_444_residues
MNHQINEWLREHRITEVECIVPDMTGIARGKIVPKDKFVSDPEMRLPEAVLIQTVTGDYPDDSLLDLTDPDMVLEPDPNT
LRYVPWAVDPTAQLIYDCCRADGSPVDVAPRNVLKRVLGLFDEMGLEPVLAPEMEFYLLSPNPDPDIPLAAPIGRTGRAE
FGRRSYSIDAVNEFDPLFEDIYDYCHAQNLEVDTLIHEIGTAQMEINFLHGNPLELADQVFLFKRTVREAAFRHNMYATF
MAKPMENEPGSAMHIHQSLVDKNTGRNVFTNEDGSPSDIFFHFIAGLQKYVPQTMPLFAPYVNSFRRLSRYTAAPTNVEW
GYDNRTVGLRIPHSSPAARRIENRVPGVDVNPYIAIAATLACGYLGMVNKLRPTEPRQTDAYELPYQFPNSSEESLLQLA
GCSEIAEVLGPRFVKMYVAMKEKEFSEYFRVISPWERKFLLLHV
>Mature_444_residues
MNHQINEWLREHRITEVECIVPDMTGIARGKIVPKDKFVSDPEMRLPEAVLIQTVTGDYPDDSLLDLTDPDMVLEPDPNT
LRYVPWAVDPTAQLIYDCCRADGSPVDVAPRNVLKRVLGLFDEMGLEPVLAPEMEFYLLSPNPDPDIPLAAPIGRTGRAE
FGRRSYSIDAVNEFDPLFEDIYDYCHAQNLEVDTLIHEIGTAQMEINFLHGNPLELADQVFLFKRTVREAAFRHNMYATF
MAKPMENEPGSAMHIHQSLVDKNTGRNVFTNEDGSPSDIFFHFIAGLQKYVPQTMPLFAPYVNSFRRLSRYTAAPTNVEW
GYDNRTVGLRIPHSSPAARRIENRVPGVDVNPYIAIAATLACGYLGMVNKLRPTEPRQTDAYELPYQFPNSSEESLLQLA
GCSEIAEVLGPRFVKMYVAMKEKEFSEYFRVISPWERKFLLLHV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=458, Percent_Identity=26.4192139737991, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI87081870, Length=435, Percent_Identity=34.7126436781609, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1790301, Length=459, Percent_Identity=27.8867102396514, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50447; Mature: 50447

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHQINEWLREHRITEVECIVPDMTGIARGKIVPKDKFVSDPEMRLPEAVLIQTVTGDYP
CCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHCCCHHHEEEECCCCCC
DDSLLDLTDPDMVLEPDPNTLRYVPWAVDPTAQLIYDCCRADGSPVDVAPRNVLKRVLGL
CCCEEECCCCCEEECCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
FDEMGLEPVLAPEMEFYLLSPNPDPDIPLAAPIGRTGRAEFGRRSYSIDAVNEFDPLFED
HHHCCCCCEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHH
IYDYCHAQNLEVDTLIHEIGTAQMEINFLHGNPLELADQVFLFKRTVREAAFRHNMYATF
HHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHEEE
MAKPMENEPGSAMHIHQSLVDKNTGRNVFTNEDGSPSDIFFHFIAGLQKYVPQTMPLFAP
EECCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
YVNSFRRLSRYTAAPTNVEWGYDNRTVGLRIPHSSPAARRIENRVPGVDVNPYIAIAATL
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHH
ACGYLGMVNKLRPTEPRQTDAYELPYQFPNSSEESLLQLAGCSEIAEVLGPRFVKMYVAM
HHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHCHHHHHHHHCHHHHHHHHHH
KEKEFSEYFRVISPWERKFLLLHV
HHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure
MNHQINEWLREHRITEVECIVPDMTGIARGKIVPKDKFVSDPEMRLPEAVLIQTVTGDYP
CCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHCCCHHHEEEECCCCCC
DDSLLDLTDPDMVLEPDPNTLRYVPWAVDPTAQLIYDCCRADGSPVDVAPRNVLKRVLGL
CCCEEECCCCCEEECCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
FDEMGLEPVLAPEMEFYLLSPNPDPDIPLAAPIGRTGRAEFGRRSYSIDAVNEFDPLFED
HHHCCCCCEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHH
IYDYCHAQNLEVDTLIHEIGTAQMEINFLHGNPLELADQVFLFKRTVREAAFRHNMYATF
HHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHEEE
MAKPMENEPGSAMHIHQSLVDKNTGRNVFTNEDGSPSDIFFHFIAGLQKYVPQTMPLFAP
EECCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
YVNSFRRLSRYTAAPTNVEWGYDNRTVGLRIPHSSPAARRIENRVPGVDVNPYIAIAATL
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHH
ACGYLGMVNKLRPTEPRQTDAYELPYQFPNSSEESLLQLAGCSEIAEVLGPRFVKMYVAM
HHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHCHHHHHHHHCHHHHHHHHHH
KEKEFSEYFRVISPWERKFLLLHV
HHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]