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The map label for this gene is puuD [H]

Identifier: 34497478

GI number: 34497478

Start: 2204308

End: 2205063

Strand: Direct

Name: puuD [H]

Synonym: CV_2023

Alternate gene names: 34497478

Gene position: 2204308-2205063 (Clockwise)

Preceding gene: 34497477

Following gene: 34497479

Centisome position: 46.4

GC content: 68.12

Gene sequence:

>756_bases
ATGTCGCAAGCAATGATAGGCATCCCGTGCGATCTGAAAATGATCGGTCTGTTGCCGTTCCACGCGGTGGGCGACAAATA
CATCGCCGCGGCGGCCGGCGGAGCGGGCGGCCTGCCGGTGTTGATCCCGTCGCTGGGCGACGAGCAGCTGCTGCGCGCCA
CGCTGGCGACGCTGGACGGCGTGCTGCTGCCGGGTTCGCCGTCCAATGTGGAGCCGCGCCATTACGGCGGGCCCAATAGC
CGTGCCGGCACGCTGCACGATCCGCGCCGCGACGCCACCACGCTGCCGCTGATCCGACTGGCGCTGGAGGAGGGCGTGCC
GCTGCTGGGCATTTGCCGCGGCTTCCAGGAGATCAATGTCGCGCTGGGCGGCGAATTGCACCAGCACGTGCAGGAAGAGC
CTGGACTGCGCGACCACCGCGAGGCGGAGGGCGACGACATTGCCGCGATGTACGCGCCGGCGCACCGGGTGGGCTTCGTC
GAAGGCGGCCTGCTGGCGGAGTGGTCCGGCGCGCGCGAGGCGATGGTCAATTCGCTGCACCAGCAGGGCATCAAGCGGCT
GGCGCCCAGCCTGATCGCCGAGGCGCATGCCGAGGACGGCCTGGTGGAGGCCTACCGGGTCCGCAATTCCAAGGGTTTCG
CTTTCGCCGTGCAATGGCATCCGGAATGGCTGTACTGGGACAATCCGCTGTCCATGGCCATTTTCCATGCCTTCGGCGAG
GCGTGCCGGGCAAGGAGGGAAAGCCGCAAGGGATGA

Upstream 100 bases:

>100_bases
GCGGGTTACATACCAGGCGGTGGCCCGCGATTTGGGCCACGCCTACGTCGATCCGATAGAGGCCATCAAGGCCGCAAACT
GACAAGGAGCGCTGCAAGCC

Downstream 100 bases:

>100_bases
ATCCCCATGCGCGCCGGACGAGGAACCCGGCGCGAGTCTTGATGACTCATAAAGTAGCACCTCCCGATCCAGAGCCGCAG
CCAGCGGCCGGGGAGACCAT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSQAMIGIPCDLKMIGLLPFHAVGDKYIAAAAGGAGGLPVLIPSLGDEQLLRATLATLDGVLLPGSPSNVEPRHYGGPNS
RAGTLHDPRRDATTLPLIRLALEEGVPLLGICRGFQEINVALGGELHQHVQEEPGLRDHREAEGDDIAAMYAPAHRVGFV
EGGLLAEWSGAREAMVNSLHQQGIKRLAPSLIAEAHAEDGLVEAYRVRNSKGFAFAVQWHPEWLYWDNPLSMAIFHAFGE
ACRARRESRKG

Sequences:

>Translated_251_residues
MSQAMIGIPCDLKMIGLLPFHAVGDKYIAAAAGGAGGLPVLIPSLGDEQLLRATLATLDGVLLPGSPSNVEPRHYGGPNS
RAGTLHDPRRDATTLPLIRLALEEGVPLLGICRGFQEINVALGGELHQHVQEEPGLRDHREAEGDDIAAMYAPAHRVGFV
EGGLLAEWSGAREAMVNSLHQQGIKRLAPSLIAEAHAEDGLVEAYRVRNSKGFAFAVQWHPEWLYWDNPLSMAIFHAFGE
ACRARRESRKG
>Mature_250_residues
SQAMIGIPCDLKMIGLLPFHAVGDKYIAAAAGGAGGLPVLIPSLGDEQLLRATLATLDGVLLPGSPSNVEPRHYGGPNSR
AGTLHDPRRDATTLPLIRLALEEGVPLLGICRGFQEINVALGGELHQHVQEEPGLRDHREAEGDDIAAMYAPAHRVGFVE
GGLLAEWSGAREAMVNSLHQQGIKRLAPSLIAEAHAEDGLVEAYRVRNSKGFAFAVQWHPEWLYWDNPLSMAIFHAFGEA
CRARRESRKG

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=225, Percent_Identity=44.4444444444444, Blast_Score=164, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 27083; Mature: 26952

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAMIGIPCDLKMIGLLPFHAVGDKYIAAAAGGAGGLPVLIPSLGDEQLLRATLATLDG
CCCCEECCCCCCCCCCCCCHHHHCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHCC
VLLPGSPSNVEPRHYGGPNSRAGTLHDPRRDATTLPLIRLALEEGVPLLGICRGFQEINV
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCHHHHHH
ALGGELHQHVQEEPGLRDHREAEGDDIAAMYAPAHRVGFVEGGLLAEWSGAREAMVNSLH
HHCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHCEECCCEEECCCCHHHHHHHHHH
QQGIKRLAPSLIAEAHAEDGLVEAYRVRNSKGFAFAVQWHPEWLYWDNPLSMAIFHAFGE
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEEECCCEEEECCHHHHHHHHHHHH
ACRARRESRKG
HHHHHHHHCCC
>Mature Secondary Structure 
SQAMIGIPCDLKMIGLLPFHAVGDKYIAAAAGGAGGLPVLIPSLGDEQLLRATLATLDG
CCCEECCCCCCCCCCCCCHHHHCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHCC
VLLPGSPSNVEPRHYGGPNSRAGTLHDPRRDATTLPLIRLALEEGVPLLGICRGFQEINV
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCHHHHHH
ALGGELHQHVQEEPGLRDHREAEGDDIAAMYAPAHRVGFVEGGLLAEWSGAREAMVNSLH
HHCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHCEECCCEEECCCCHHHHHHHHHH
QQGIKRLAPSLIAEAHAEDGLVEAYRVRNSKGFAFAVQWHPEWLYWDNPLSMAIFHAFGE
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEEECCCEEEECCHHHHHHHHHHHH
ACRARRESRKG
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]