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The map label for this gene is argG

Identifier: 34497449

GI number: 34497449

Start: 2174588

End: 2175814

Strand: Direct

Name: argG

Synonym: CV_1994

Alternate gene names: 34497449

Gene position: 2174588-2175814 (Clockwise)

Preceding gene: 34497448

Following gene: 34497450

Centisome position: 45.77

GC content: 62.92

Gene sequence:

>1227_bases
ATGTCTGACATCAAGAAAGTGGTTTTGGCGTATTCCGGCGGTCTGGATACCTCGGTGATCCTGAAGTGGCTGCAAGACGA
GTATCAATGCGAAGTGGTGACCTTCACCGCCGACATCGGCCAGGGCGAGGAAGTGGAGCCGGCGCGGCAGAAAGCGGTTT
CGCTGGGCATCAAGCCGGAAAACATCTTCATCGAGGACCTGCGCGAGGAGTTCGTCCGCGATTTCGTCTACCCGATGTTC
CGCGCCAACACTATTTACGAAGGCGAATACCTGCTGGGCACCTCGATCGCCCGGCCGCTGATCGCCAAGCGTCAGATCGA
GATCGCCAACCAGGTTGGCGCCGACGCGGTATCGCACGGCGCCACCGGCAAGGGCAACGACCAGGTGCGCTTCGAGCTCG
GTTATTACGCGCTGAAGCCGGATGTGAAAGTGATCGCGCCGTGGCGCGAGTGGGACCTGCTGTCGCGCGAGAAGCTGCTG
GCCTACGCCGAAACCCACGGCATCGACATCAGCAAGAAGAAGAACGGCGGCAGTCCGTACTCGATGGACGCCAATCTGCT
GCACATTTCCTACGAAGGCACGGTGCTGGAAGATCCGGCCCAGGAGCCGGAAGAGGATATGTGGCTGTGGAGCGTGAGTC
CGGAGAACGCGCCGGACCAGGCCGAGTACGTGGAGCTGGAATACCGCAAGGGCGACATCGTCGCCGTCAACGGCCAAGCG
CTGAGCCCGGCCGGCGTGCTGACCGAGCTGAACCGCCTGGGCAACAAGCACGGCATCGGCCGCTTGGACATCGTCGAGAA
CCGCTACGTGGGCATGAAGTCGCGCGGCTGCTATGAAACCCCGGGCGGCACCATCATGCTGAAGGCGCACCGCGCGATCG
AGTCCATCACGCTGGACCGCGAAGTGGCCCACCTGAAGGACGAGCTGATGCCGAAATACGCGCAGCTGATCTACACCGGT
TACTGGTGGAGCCCGGAGCGCGCGATGCTGCAGCAGATGATAGACGCGTCGCAGGCCACGGTGAACGGCTGGGTGCGGCT
GAAGCTGTACAAGGGCAATGTGATCGTCGTCGGCCGCGAATCCAAGACCGATTCGCTGTTCGACCCGACCATCGCCACCT
TCGACGAGGACGGCGGCGCCTACAACCATGCCGACGCGGCCGGCTTCATCCGCCTGAACGCGCTGCGGATGCGCATCGCT
GCCAATGCGCGGAACAAGCGCGGCTGA

Upstream 100 bases:

>100_bases
CGTGCAGAAGGCCTTGATCGAATATCTGTTGCTGGGGCATCGCGAGGACTGAGTCCCGCCCGGCAACCGGATAAATAAAC
AACGAGTGGAAAGAGAAGCA

Downstream 100 bases:

>100_bases
TATCATCTATTCATACCGTTGACATGGCGGCCGTGCGGCCGCCATTTTTCGTTCTTTGTACAGAGGGAGTGCCGCGGGAT
GCGGGCGAGCAGCTGGATTC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSDIKKVVLAYSGGLDTSVILKWLQDEYQCEVVTFTADIGQGEEVEPARQKAVSLGIKPENIFIEDLREEFVRDFVYPMF
RANTIYEGEYLLGTSIARPLIAKRQIEIANQVGADAVSHGATGKGNDQVRFELGYYALKPDVKVIAPWREWDLLSREKLL
AYAETHGIDISKKKNGGSPYSMDANLLHISYEGTVLEDPAQEPEEDMWLWSVSPENAPDQAEYVELEYRKGDIVAVNGQA
LSPAGVLTELNRLGNKHGIGRLDIVENRYVGMKSRGCYETPGGTIMLKAHRAIESITLDREVAHLKDELMPKYAQLIYTG
YWWSPERAMLQQMIDASQATVNGWVRLKLYKGNVIVVGRESKTDSLFDPTIATFDEDGGAYNHADAAGFIRLNALRMRIA
ANARNKRG

Sequences:

>Translated_408_residues
MSDIKKVVLAYSGGLDTSVILKWLQDEYQCEVVTFTADIGQGEEVEPARQKAVSLGIKPENIFIEDLREEFVRDFVYPMF
RANTIYEGEYLLGTSIARPLIAKRQIEIANQVGADAVSHGATGKGNDQVRFELGYYALKPDVKVIAPWREWDLLSREKLL
AYAETHGIDISKKKNGGSPYSMDANLLHISYEGTVLEDPAQEPEEDMWLWSVSPENAPDQAEYVELEYRKGDIVAVNGQA
LSPAGVLTELNRLGNKHGIGRLDIVENRYVGMKSRGCYETPGGTIMLKAHRAIESITLDREVAHLKDELMPKYAQLIYTG
YWWSPERAMLQQMIDASQATVNGWVRLKLYKGNVIVVGRESKTDSLFDPTIATFDEDGGAYNHADAAGFIRLNALRMRIA
ANARNKRG
>Mature_407_residues
SDIKKVVLAYSGGLDTSVILKWLQDEYQCEVVTFTADIGQGEEVEPARQKAVSLGIKPENIFIEDLREEFVRDFVYPMFR
ANTIYEGEYLLGTSIARPLIAKRQIEIANQVGADAVSHGATGKGNDQVRFELGYYALKPDVKVIAPWREWDLLSREKLLA
YAETHGIDISKKKNGGSPYSMDANLLHISYEGTVLEDPAQEPEEDMWLWSVSPENAPDQAEYVELEYRKGDIVAVNGQAL
SPAGVLTELNRLGNKHGIGRLDIVENRYVGMKSRGCYETPGGTIMLKAHRAIESITLDREVAHLKDELMPKYAQLIYTGY
WWSPERAMLQQMIDASQATVNGWVRLKLYKGNVIVVGRESKTDSLFDPTIATFDEDGGAYNHADAAGFIRLNALRMRIAA
NARNKRG

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=407, Percent_Identity=46.9287469287469, Blast_Score=351, Evalue=8e-97,
Organism=Homo sapiens, GI16950633, Length=407, Percent_Identity=46.9287469287469, Blast_Score=351, Evalue=8e-97,
Organism=Escherichia coli, GI1789563, Length=414, Percent_Identity=26.3285024154589, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324514, Length=417, Percent_Identity=45.8033573141487, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI21358151, Length=404, Percent_Identity=44.3069306930693, Blast_Score=338, Evalue=3e-93,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_CHRVO (Q7NWJ5)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901664.1
- ProteinModelPortal:   Q7NWJ5
- SMR:   Q7NWJ5
- GeneID:   2549053
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1994
- NMPDR:   fig|243365.1.peg.1994
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- PhylomeDB:   Q7NWJ5
- ProtClustDB:   PRK00509
- BioCyc:   CVIO243365:CV_1994-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45668; Mature: 45537

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 37-37 BINDING 90-90 BINDING 95-95 BINDING 120-120 BINDING 122-122 BINDING 126-126 BINDING 126-126 BINDING 127-127 BINDING 130-130 BINDING 181-181 BINDING 190-190 BINDING 266-266 BINDING 278-278

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIKKVVLAYSGGLDTSVILKWLQDEYQCEVVTFTADIGQGEEVEPARQKAVSLGIKPE
CCCHHEEHEEECCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHCCCCCCC
NIFIEDLREEFVRDFVYPMFRANTIYEGEYLLGTSIARPLIAKRQIEIANQVGADAVSHG
CHHHHHHHHHHHHHHHHHHHHCCEEECCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHCC
ATGKGNDQVRFELGYYALKPDVKVIAPWREWDLLSREKLLAYAETHGIDISKKKNGGSPY
CCCCCCCEEEEEEEEEEECCCCEEEECCHHCCHHHHHHHHEEHHHCCCEEEECCCCCCCE
SMDANLLHISYEGTVLEDPAQEPEEDMWLWSVSPENAPDQAEYVELEYRKGDIVAVNGQA
EECCEEEEEEECCEEECCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCEEEECCCC
LSPAGVLTELNRLGNKHGIGRLDIVENRYVGMKSRGCYETPGGTIMLKAHRAIESITLDR
CCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHCHH
EVAHLKDELMPKYAQLIYTGYWWSPERAMLQQMIDASQATVNGWVRLKLYKGNVIVVGRE
HHHHHHHHHHHHHHHHEEEECEECHHHHHHHHHHHHCCCEEEEEEEEEEEECCEEEEECC
SKTDSLFDPTIATFDEDGGAYNHADAAGFIRLNALRMRIAANARNKRG
CCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCC
>Mature Secondary Structure 
SDIKKVVLAYSGGLDTSVILKWLQDEYQCEVVTFTADIGQGEEVEPARQKAVSLGIKPE
CCHHEEHEEECCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHCCCCCCC
NIFIEDLREEFVRDFVYPMFRANTIYEGEYLLGTSIARPLIAKRQIEIANQVGADAVSHG
CHHHHHHHHHHHHHHHHHHHHCCEEECCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHCC
ATGKGNDQVRFELGYYALKPDVKVIAPWREWDLLSREKLLAYAETHGIDISKKKNGGSPY
CCCCCCCEEEEEEEEEEECCCCEEEECCHHCCHHHHHHHHEEHHHCCCEEEECCCCCCCE
SMDANLLHISYEGTVLEDPAQEPEEDMWLWSVSPENAPDQAEYVELEYRKGDIVAVNGQA
EECCEEEEEEECCEEECCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCEEEECCCC
LSPAGVLTELNRLGNKHGIGRLDIVENRYVGMKSRGCYETPGGTIMLKAHRAIESITLDR
CCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHCHH
EVAHLKDELMPKYAQLIYTGYWWSPERAMLQQMIDASQATVNGWVRLKLYKGNVIVVGRE
HHHHHHHHHHHHHHHHEEEECEECHHHHHHHHHHHHCCCEEEEEEEEEEEECCEEEEECC
SKTDSLFDPTIATFDEDGGAYNHADAAGFIRLNALRMRIAANARNKRG
CCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782