Click here to switch to the map view.

The map label for this gene is argI

Identifier: 34497448

GI number: 34497448

Start: 2173616

End: 2174539

Strand: Direct

Name: argI

Synonym: CV_1993

Alternate gene names: 34497448

Gene position: 2173616-2174539 (Clockwise)

Preceding gene: 34497447

Following gene: 34497449

Centisome position: 45.75

GC content: 63.85

Gene sequence:

>924_bases
ATGACGTCCGTAAGGCATTACCTGCAATTCAGCGACCTCTCTCCCGACGAGTATCACCATCTCTTCGAGCGAAGCCGGGT
CCTGAAGCGACGACAAAGCGCCGGGGAGCTGCATCGGCCGCTGGTCGGCAAGGTGATGTCCATGGTGTTCGAAAAGAATT
CCACCCGCACCCGCGTCTCTTTCGAGGCCGGCATGGCCCAGCTGGGCGGCCACGCGATGTTCCTGGACACCAAGAGCTCG
CAGATCGGCCGCGGCGAGCCGATAGAGGACACCGCGCGCGTGCTGTCCCGGATGAGCGACATCATCATGATCCGCACCTT
CGAGCAGGGCTTGGTGCAGCGCCTGGCCGCGCATTCGCGGGTGCCGGTGATCAACGGCCTGACCAACGAGTACCACCCTT
GCCAGGTGCTGGCCGACATCTTCACCTACGTGGAGCGCCACGGTTCGATCAAGGGCAAGACGGTGGCCTGGGTGGGTGAC
GGCAACAATGTCTGCCGCACCTGGCTGCAGGCCGCCGCCGTGCTCGGCTTCAAGCTGAAGGTGGCGTCGCCGCTCGGTTA
CGAGCTCCAGACTCTGGATGGCCGCCATTACGGTTCCGACGTGCTGGAAATGACTCGGGATCCGGCGCGTGCCGTTCACG
GAGCGGACATCGTCACCACTGACGTGTTTACCAGCATGGGTTTCGAAGCCGAACAGGCGGCGCGGCGCGAGGCTTTCGAA
GGCTACCAGGTGACGGCGGAACTGATGAAGCAGGCCAAGCCGGAAGCGCTGTTCATGCACTGCCTGCCCGCCCATCGCGG
CGAGGAGGTGGCGGCCGAGGTGATAGACGGCCCGCAGAGCGTGGTCTGGGACGAGGCAGAGAACCGCATGCACGTGCAGA
AGGCCTTGATCGAATATCTGTTGCTGGGGCATCGCGAGGACTGA

Upstream 100 bases:

>100_bases
GTAAAGTGACTTGAGACCAGCGTGTAGTAATGTGCCGTTGCGCATGAAACCACCACTCGGTGTTGCACGCGCAGCGGCCT
CTTTTTTTGATTGGAGCATC

Downstream 100 bases:

>100_bases
GTCCCGCCCGGCAACCGGATAAATAAACAACGAGTGGAAAGAGAAGCAATGTCTGACATCAAGAAAGTGGTTTTGGCGTA
TTCCGGCGGTCTGGATACCT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTSVRHYLQFSDLSPDEYHHLFERSRVLKRRQSAGELHRPLVGKVMSMVFEKNSTRTRVSFEAGMAQLGGHAMFLDTKSS
QIGRGEPIEDTARVLSRMSDIIMIRTFEQGLVQRLAAHSRVPVINGLTNEYHPCQVLADIFTYVERHGSIKGKTVAWVGD
GNNVCRTWLQAAAVLGFKLKVASPLGYELQTLDGRHYGSDVLEMTRDPARAVHGADIVTTDVFTSMGFEAEQAARREAFE
GYQVTAELMKQAKPEALFMHCLPAHRGEEVAAEVIDGPQSVVWDEAENRMHVQKALIEYLLLGHRED

Sequences:

>Translated_307_residues
MTSVRHYLQFSDLSPDEYHHLFERSRVLKRRQSAGELHRPLVGKVMSMVFEKNSTRTRVSFEAGMAQLGGHAMFLDTKSS
QIGRGEPIEDTARVLSRMSDIIMIRTFEQGLVQRLAAHSRVPVINGLTNEYHPCQVLADIFTYVERHGSIKGKTVAWVGD
GNNVCRTWLQAAAVLGFKLKVASPLGYELQTLDGRHYGSDVLEMTRDPARAVHGADIVTTDVFTSMGFEAEQAARREAFE
GYQVTAELMKQAKPEALFMHCLPAHRGEEVAAEVIDGPQSVVWDEAENRMHVQKALIEYLLLGHRED
>Mature_306_residues
TSVRHYLQFSDLSPDEYHHLFERSRVLKRRQSAGELHRPLVGKVMSMVFEKNSTRTRVSFEAGMAQLGGHAMFLDTKSSQ
IGRGEPIEDTARVLSRMSDIIMIRTFEQGLVQRLAAHSRVPVINGLTNEYHPCQVLADIFTYVERHGSIKGKTVAWVGDG
NNVCRTWLQAAAVLGFKLKVASPLGYELQTLDGRHYGSDVLEMTRDPARAVHGADIVTTDVFTSMGFEAEQAARREAFEG
YQVTAELMKQAKPEALFMHCLPAHRGEEVAAEVIDGPQSVVWDEAENRMHVQKALIEYLLLGHRED

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=305, Percent_Identity=41.6393442622951, Blast_Score=234, Evalue=8e-62,
Organism=Homo sapiens, GI18105007, Length=319, Percent_Identity=29.153605015674, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=38.8379204892966, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI1786469, Length=327, Percent_Identity=39.1437308868502, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI2367364, Length=314, Percent_Identity=28.6624203821656, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI48994908, Length=364, Percent_Identity=24.1758241758242, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=335, Percent_Identity=29.8507462686567, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6322373, Length=321, Percent_Identity=32.7102803738318, Blast_Score=188, Evalue=9e-49,
Organism=Saccharomyces cerevisiae, GI6322331, Length=283, Percent_Identity=29.3286219081272, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24642586, Length=320, Percent_Identity=30.625, Blast_Score=122, Evalue=2e-28,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_CHRVO (Q7NWJ6)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901663.1
- ProteinModelPortal:   Q7NWJ6
- SMR:   Q7NWJ6
- GeneID:   2548987
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1993
- NMPDR:   fig|243365.1.peg.1993
- HOGENOM:   HBG579429
- OMA:   CVVTDTW
- PhylomeDB:   Q7NWJ6
- ProtClustDB:   PRK00779
- BioCyc:   CVIO243365:CV_1993-MONOMER
- BRENDA:   2.1.3.3
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 34479; Mature: 34348

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 105-105 BINDING 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVRHYLQFSDLSPDEYHHLFERSRVLKRRQSAGELHRPLVGKVMSMVFEKNSTRTRVS
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
FEAGMAQLGGHAMFLDTKSSQIGRGEPIEDTARVLSRMSDIIMIRTFEQGLVQRLAAHSR
HHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VPVINGLTNEYHPCQVLADIFTYVERHGSIKGKTVAWVGDGNNVCRTWLQAAAVLGFKLK
CCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEE
VASPLGYELQTLDGRHYGSDVLEMTRDPARAVHGADIVTTDVFTSMGFEAEQAARREAFE
ECCCCCCEEEECCCCCCCHHHHHHHCCHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHC
GYQVTAELMKQAKPEALFMHCLPAHRGEEVAAEVIDGPQSVVWDEAENRMHVQKALIEYL
CHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LLGHRED
HHCCCCC
>Mature Secondary Structure 
TSVRHYLQFSDLSPDEYHHLFERSRVLKRRQSAGELHRPLVGKVMSMVFEKNSTRTRVS
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
FEAGMAQLGGHAMFLDTKSSQIGRGEPIEDTARVLSRMSDIIMIRTFEQGLVQRLAAHSR
HHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VPVINGLTNEYHPCQVLADIFTYVERHGSIKGKTVAWVGDGNNVCRTWLQAAAVLGFKLK
CCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEE
VASPLGYELQTLDGRHYGSDVLEMTRDPARAVHGADIVTTDVFTSMGFEAEQAARREAFE
ECCCCCCEEEECCCCCCCHHHHHHHCCHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHC
GYQVTAELMKQAKPEALFMHCLPAHRGEEVAAEVIDGPQSVVWDEAENRMHVQKALIEYL
CHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LLGHRED
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782