Click here to switch to the map view.

The map label for this gene is potF3 [H]

Identifier: 34497412

GI number: 34497412

Start: 2129000

End: 2130100

Strand: Direct

Name: potF3 [H]

Synonym: CV_1957

Alternate gene names: 34497412

Gene position: 2129000-2130100 (Clockwise)

Preceding gene: 34497410

Following gene: 34497413

Centisome position: 44.81

GC content: 62.03

Gene sequence:

>1101_bases
ATGAAGAAGAAACTGGTTCGCAACGCGCTGTATTCGCTGCTGTTGCTGCTGCCGGCCGCCGGCGCGCTGGCGGCGGACAA
GGTGCTCAACGTCTACAACTGGTCCGACTACATCGCCAAGACCACCATCCCGGGCTTCGAAAAGCAGACCGGGGTCAAGG
TGAAATACGATGTCTACGACAGCGACGACACCTTGCAGGCCAAGATGCTGACCGGCCGCGCCGGCTACGACATCGTGGTG
CCCACATCCAATTTCATGGCCAAGCAGATCGAGGCCGGCATCTACCAGAAGCTGGACAAGTCCAAAATCCCCAATCTGTC
GCATCTGGACAAGGCGCTGATGGCCAAGATCGTCGACGCCGATCCCGGCAACGCCCGCGGCGTGCCTTGGGCCTACGGCA
CCGACGGCCTGGGCTACAACTACACCAAGGTCAAAGCGCTGCTGGGCGCCGGCGCGCCGCTGGACAGCTGGGACATCCTG
TTCGATCCGAAATACGTGTCCAAGCTGAAGGGCTGCGGCGTATCGGTGCTGGACCAGGCCAACGACGTGTTCGCCGTCAC
CCTTCACCACTTGGGCAAGGATCCGAACAGCAAGAATCCCGCCGACTACCAGGCCGCGTTCGAGGCCTTGAAGAAGATCC
GCCCCTACATCACCCAGTTCAACTCGTCTGGCTACATCAACGACCTGGCCAACGGCGACATCTGCCTGGCGCTGGGCTGG
TCAGGCGACGTCAACATCGCCAAGCACCGCGCCGCCGAAGCCAAGCGCTCCTATCAGCTGGGCTACGTGATTCCCAAGTC
CGGCGCGCCGATCTGGTTCGACGTGATGGTGATCCCGAAAGACGCGCCGCATCCGGAAGAGGCGCACAAGTGGATCAACT
ACATCGAGACGCCCGAGGTGAACGCCGAGATCACCAACGAGGTGTTCTATCCCACCGTCAACGCGGCGGCGCGCAAGTAC
GTGAAGCCCGAGATCGCCAGCGATCCCACCATCTATCCGCCGGAGTCGGTGATGAAGACGCTGTTCCTGCTCAAGCCGCT
GCCGCCTGACATCATGCGGCTGCAGAACCGGCTGTGGACCCAGCTGAAGACCGGCCGCTGA

Upstream 100 bases:

>100_bases
TATTCGGCATGTCCATATGACGCGCGTCGCCGCGTCGGGATCGCCGCAGGCCGCTCCGGGACGGGGACGTTTATGGTCGT
GCCACTTTAGGAAGCCTGTC

Downstream 100 bases:

>100_bases
GCGCGCTTCCCGCCGGGCGGTTTGGCCCGGCTTTCGCAAGCAGATAAAACAGTCCGCCGCCCGGCTGGTCCGGGACGGCA
GGACAGCCGTGGAACATCGA

Product: polyamine ABC transporter system, substrate-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKKKLVRNALYSLLLLLPAAGALAADKVLNVYNWSDYIAKTTIPGFEKQTGVKVKYDVYDSDDTLQAKMLTGRAGYDIVV
PTSNFMAKQIEAGIYQKLDKSKIPNLSHLDKALMAKIVDADPGNARGVPWAYGTDGLGYNYTKVKALLGAGAPLDSWDIL
FDPKYVSKLKGCGVSVLDQANDVFAVTLHHLGKDPNSKNPADYQAAFEALKKIRPYITQFNSSGYINDLANGDICLALGW
SGDVNIAKHRAAEAKRSYQLGYVIPKSGAPIWFDVMVIPKDAPHPEEAHKWINYIETPEVNAEITNEVFYPTVNAAARKY
VKPEIASDPTIYPPESVMKTLFLLKPLPPDIMRLQNRLWTQLKTGR

Sequences:

>Translated_366_residues
MKKKLVRNALYSLLLLLPAAGALAADKVLNVYNWSDYIAKTTIPGFEKQTGVKVKYDVYDSDDTLQAKMLTGRAGYDIVV
PTSNFMAKQIEAGIYQKLDKSKIPNLSHLDKALMAKIVDADPGNARGVPWAYGTDGLGYNYTKVKALLGAGAPLDSWDIL
FDPKYVSKLKGCGVSVLDQANDVFAVTLHHLGKDPNSKNPADYQAAFEALKKIRPYITQFNSSGYINDLANGDICLALGW
SGDVNIAKHRAAEAKRSYQLGYVIPKSGAPIWFDVMVIPKDAPHPEEAHKWINYIETPEVNAEITNEVFYPTVNAAARKY
VKPEIASDPTIYPPESVMKTLFLLKPLPPDIMRLQNRLWTQLKTGR
>Mature_366_residues
MKKKLVRNALYSLLLLLPAAGALAADKVLNVYNWSDYIAKTTIPGFEKQTGVKVKYDVYDSDDTLQAKMLTGRAGYDIVV
PTSNFMAKQIEAGIYQKLDKSKIPNLSHLDKALMAKIVDADPGNARGVPWAYGTDGLGYNYTKVKALLGAGAPLDSWDIL
FDPKYVSKLKGCGVSVLDQANDVFAVTLHHLGKDPNSKNPADYQAAFEALKKIRPYITQFNSSGYINDLANGDICLALGW
SGDVNIAKHRAAEAKRSYQLGYVIPKSGAPIWFDVMVIPKDAPHPEEAHKWINYIETPEVNAEITNEVFYPTVNAAARKY
VKPEIASDPTIYPPESVMKTLFLLKPLPPDIMRLQNRLWTQLKTGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=354, Percent_Identity=48.3050847457627, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI1787367, Length=315, Percent_Identity=33.968253968254, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1787711, Length=333, Percent_Identity=24.3243243243243, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40521; Mature: 40521

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLVRNALYSLLLLLPAAGALAADKVLNVYNWSDYIAKTTIPGFEKQTGVKVKYDVYD
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCEEEEEEEC
SDDTLQAKMLTGRAGYDIVVPTSNFMAKQIEAGIYQKLDKSKIPNLSHLDKALMAKIVDA
CCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
DPGNARGVPWAYGTDGLGYNYTKVKALLGAGAPLDSWDILFDPKYVSKLKGCGVSVLDQA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHCCCCHHHHCC
NDVFAVTLHHLGKDPNSKNPADYQAAFEALKKIRPYITQFNSSGYINDLANGDICLALGW
CCEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEECC
SGDVNIAKHRAAEAKRSYQLGYVIPKSGAPIWFDVMVIPKDAPHPEEAHKWINYIETPEV
CCCCHHHHHHHHHHHHCEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCC
NAEITNEVFYPTVNAAARKYVKPEIASDPTIYPPESVMKTLFLLKPLPPDIMRLQNRLWT
CCCCCCCEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
QLKTGR
HHCCCC
>Mature Secondary Structure
MKKKLVRNALYSLLLLLPAAGALAADKVLNVYNWSDYIAKTTIPGFEKQTGVKVKYDVYD
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCEEEEEEEC
SDDTLQAKMLTGRAGYDIVVPTSNFMAKQIEAGIYQKLDKSKIPNLSHLDKALMAKIVDA
CCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
DPGNARGVPWAYGTDGLGYNYTKVKALLGAGAPLDSWDILFDPKYVSKLKGCGVSVLDQA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHCCCCHHHHCC
NDVFAVTLHHLGKDPNSKNPADYQAAFEALKKIRPYITQFNSSGYINDLANGDICLALGW
CCEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEECC
SGDVNIAKHRAAEAKRSYQLGYVIPKSGAPIWFDVMVIPKDAPHPEEAHKWINYIETPEV
CCCCHHHHHHHHHHHHCEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCC
NAEITNEVFYPTVNAAARKYVKPEIASDPTIYPPESVMKTLFLLKPLPPDIMRLQNRLWT
CCCCCCCEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
QLKTGR
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]