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The map label for this gene is mnmC

Identifier: 34497397

GI number: 34497397

Start: 2112644

End: 2114626

Strand: Reverse

Name: mnmC

Synonym: CV_1942

Alternate gene names: 34497397

Gene position: 2114626-2112644 (Counterclockwise)

Preceding gene: 34497398

Following gene: 34497395

Centisome position: 44.51

GC content: 71.36

Gene sequence:

>1983_bases
ATGACCCACTCTCACGCCCAACTCGTCTGGCAGGACGGCCAGCCCATGTCCGCCGTCTTCGGCGACGTGTATTTCTCCCG
CGCCTCCGGCCTGGAGGAGACCCGCCACGTCTTTCTCCGCCACAATCAGTTGGAGGAGCGCTTCGCCGCGCTGCCCCCCT
CCGGCTCATTCGCCATCGCGGAAACCGGCTTCGGCACCGGCCTGAATTTCCTCTGCGCCTGGCAATGCTTCGAGGCGCGC
GCGCCGGCCGGCGCCAGGCTGCACTTCGTCAGCGCGGAGAAGTTTCCGCTGACCCCGTCCGACCTGGCCCAGGCGCTGGC
GCTGTGGCCGGAGCTGGAGCCATGGTCTTCCCAGCTGCTGGCGCAATACGACGTTCTGACGCCGGGCTGGCACCGCTTCG
TGCTCGCCGGCGGCAGGGTGACGCTGACGCTGATGATAGGCGACGTGCTGGAGGTGCTGCCCCAGCTGGATGCACGCGTC
GACGCCTGGTTCCTGGACGGATTCGCGCCGTCCAAAAACCCGGACATGTGGCAGCAGGCGCTGTTCGAGCAGATGGCCCG
CCTGTCGGGTCCCGGCGCCAGCTTCGCCACTTTCACCAGCGCGGGCGCGGTGCGGCGCGGCCTGGCCGGGGCCGGTTTCG
AAGTCAGGAAAGCGCCCGGCCACGGCGCCAAGCGCCATATCAGCCACGGCTGGATCGCCACGCCGCCTGACGCCGGCTGG
CAAGCGCCTTGGTACGCCCGGCCCGAGCCCCGCTGGCGCGAGCGCAGCGCCATCGTGGTCGGCGGCGGCCTGGCCGGCGC
CGCCAGCGCGCGCAGCCTGGCGCTGCGCGGCTGGCAGGTGACGCTGATAGAGCGGATGCCGCAATTGGCGAGCGCGGCCT
CCGGCAATCCGCAGGGCGTGCTGTACACCAAGCTCTCCCCCCACCTGACGCCGCTGACCCGGCTGGTGCTGTCCGGCTAC
GCCTACAGCCTGCGCGCGCTGCGCTGCCTGCTGCCCGAGGACGGCGACTGGCAGGCCTGCGGCGTGCTGCAGCTGGCGCA
TGACGGCAAGGAAGCGGAAAAACAGCAGGCGCTGGCCGAGTTGAACCTGCCGGAATCGGTGATGCGGCCGCTGGATCGGG
ACGCCGCCAGCGAGCTGGCCGGCACGGATCTGCCATGCGGCGGATTGTTCTTCCCCCAGGGCGGCTGGGTGCATCCGCCC
GCGCTGGTGCGCCAGCTGGCCGACCACCCGAATATCGTGATCAAGACCGGCCGCACCGCGTTGACGCTGGATTACGATCC
GGCGCAACGCAGCTGGACCGCCGGCGACGAGCAGGGGCCGCTGGCGGTGGCGTCCGTGGTGGTGCTGGCCGGCGCGGCCG
AAACCGCGCAGTTCGACAGCACCCGCCATCTGCCGCTGAAAAAGATCCGCGGCCAGATCACCAGCATCCCCGCCAGCGAG
GAAAGCAGCCGGCTGCGCACCGTGCTCTGCGGCGAAGGCTATATCTCCCCCGCGCGCGGCGGCCGCCATTGCCTGGGCGC
CACCTTCAAGTTCGACACCGACGACCTTGGCGTCAACGACGGCGAGCATGCGGAAAACCTGGCCATGCTGGCCGAACTGG
CCCCGTCGCTGCGCGCCAGCCTGGACCGCGACGCGCCTGAGCACCTGGACGGGCGCGCCGCCTTCCGCTGCACCAGCCCA
GACTACCTGCCGCTGGTCGGCCCCGCGGTGGCGGCGCGCGATTTCGTCCACGCCTACCGCGAACTGGCGCGCGACGCGAC
CTTGCGGCCGGCCACGGCCTGCCCGTGGGCCGAGGGCCTGTACGTCAACGCCGCCCACGGCTCGCGCGGGCTGATCACCG
CGCCGCTGTCGGGCGAGATACTGGCTTCGCTGCTGGAGGGCGAGCCGGCGCCGCTGCCCGCCGATCTGATGCGGGCGGTG
CATCCGTCCCGTTTCCTGCTGCGCGACCTGATCCGCCGCAAGCTGGACCCGGACGCGCTGTGA

Upstream 100 bases:

>100_bases
GAGGCGCTTGAACTTGGACGCTCATGCCCGGCATGAGACAATGGCGTCAAATCGATTTACCCTTCCCGCCGGCCCTCCAT
CGCCGACGCTGATCAAATAA

Downstream 100 bases:

>100_bases
CCCATCTCAGGCCGCGGTCGGCGCGCGCGTTATCGCCGCTCGGATCGCGTCCGGGATCGGCACCGACTTCTCGGCCTTGT
AATCCACCCAGACCAGCAGC

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MTHSHAQLVWQDGQPMSAVFGDVYFSRASGLEETRHVFLRHNQLEERFAALPPSGSFAIAETGFGTGLNFLCAWQCFEAR
APAGARLHFVSAEKFPLTPSDLAQALALWPELEPWSSQLLAQYDVLTPGWHRFVLAGGRVTLTLMIGDVLEVLPQLDARV
DAWFLDGFAPSKNPDMWQQALFEQMARLSGPGASFATFTSAGAVRRGLAGAGFEVRKAPGHGAKRHISHGWIATPPDAGW
QAPWYARPEPRWRERSAIVVGGGLAGAASARSLALRGWQVTLIERMPQLASAASGNPQGVLYTKLSPHLTPLTRLVLSGY
AYSLRALRCLLPEDGDWQACGVLQLAHDGKEAEKQQALAELNLPESVMRPLDRDAASELAGTDLPCGGLFFPQGGWVHPP
ALVRQLADHPNIVIKTGRTALTLDYDPAQRSWTAGDEQGPLAVASVVVLAGAAETAQFDSTRHLPLKKIRGQITSIPASE
ESSRLRTVLCGEGYISPARGGRHCLGATFKFDTDDLGVNDGEHAENLAMLAELAPSLRASLDRDAPEHLDGRAAFRCTSP
DYLPLVGPAVAARDFVHAYRELARDATLRPATACPWAEGLYVNAAHGSRGLITAPLSGEILASLLEGEPAPLPADLMRAV
HPSRFLLRDLIRRKLDPDAL

Sequences:

>Translated_660_residues
MTHSHAQLVWQDGQPMSAVFGDVYFSRASGLEETRHVFLRHNQLEERFAALPPSGSFAIAETGFGTGLNFLCAWQCFEAR
APAGARLHFVSAEKFPLTPSDLAQALALWPELEPWSSQLLAQYDVLTPGWHRFVLAGGRVTLTLMIGDVLEVLPQLDARV
DAWFLDGFAPSKNPDMWQQALFEQMARLSGPGASFATFTSAGAVRRGLAGAGFEVRKAPGHGAKRHISHGWIATPPDAGW
QAPWYARPEPRWRERSAIVVGGGLAGAASARSLALRGWQVTLIERMPQLASAASGNPQGVLYTKLSPHLTPLTRLVLSGY
AYSLRALRCLLPEDGDWQACGVLQLAHDGKEAEKQQALAELNLPESVMRPLDRDAASELAGTDLPCGGLFFPQGGWVHPP
ALVRQLADHPNIVIKTGRTALTLDYDPAQRSWTAGDEQGPLAVASVVVLAGAAETAQFDSTRHLPLKKIRGQITSIPASE
ESSRLRTVLCGEGYISPARGGRHCLGATFKFDTDDLGVNDGEHAENLAMLAELAPSLRASLDRDAPEHLDGRAAFRCTSP
DYLPLVGPAVAARDFVHAYRELARDATLRPATACPWAEGLYVNAAHGSRGLITAPLSGEILASLLEGEPAPLPADLMRAV
HPSRFLLRDLIRRKLDPDAL
>Mature_659_residues
THSHAQLVWQDGQPMSAVFGDVYFSRASGLEETRHVFLRHNQLEERFAALPPSGSFAIAETGFGTGLNFLCAWQCFEARA
PAGARLHFVSAEKFPLTPSDLAQALALWPELEPWSSQLLAQYDVLTPGWHRFVLAGGRVTLTLMIGDVLEVLPQLDARVD
AWFLDGFAPSKNPDMWQQALFEQMARLSGPGASFATFTSAGAVRRGLAGAGFEVRKAPGHGAKRHISHGWIATPPDAGWQ
APWYARPEPRWRERSAIVVGGGLAGAASARSLALRGWQVTLIERMPQLASAASGNPQGVLYTKLSPHLTPLTRLVLSGYA
YSLRALRCLLPEDGDWQACGVLQLAHDGKEAEKQQALAELNLPESVMRPLDRDAASELAGTDLPCGGLFFPQGGWVHPPA
LVRQLADHPNIVIKTGRTALTLDYDPAQRSWTAGDEQGPLAVASVVVLAGAAETAQFDSTRHLPLKKIRGQITSIPASEE
SSRLRTVLCGEGYISPARGGRHCLGATFKFDTDDLGVNDGEHAENLAMLAELAPSLRASLDRDAPEHLDGRAAFRCTSPD
YLPLVGPAVAARDFVHAYRELARDATLRPATACPWAEGLYVNAAHGSRGLITAPLSGEILASLLEGEPAPLPADLMRAVH
PSRFLLRDLIRRKLDPDAL

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=659, Percent_Identity=40.9711684370258, Blast_Score=436, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_CHRVO (Q7NWN9)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901612.1
- ProteinModelPortal:   Q7NWN9
- SMR:   Q7NWN9
- GeneID:   2548639
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1942
- NMPDR:   fig|243365.1.peg.1942
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- PhylomeDB:   Q7NWN9
- ProtClustDB:   PRK01747
- BioCyc:   CVIO243365:CV_1942-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 71302; Mature: 71171

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00047 HISTONE_H4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSHAQLVWQDGQPMSAVFGDVYFSRASGLEETRHVFLRHNQLEERFAALPPSGSFAIA
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
ETGFGTGLNFLCAWQCFEARAPAGARLHFVSAEKFPLTPSDLAQALALWPELEPWSSQLL
ECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHH
AQYDVLTPGWHRFVLAGGRVTLTLMIGDVLEVLPQLDARVDAWFLDGFAPSKNPDMWQQA
HHHCCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHCCHHCEEEECCCCCCCCHHHHHHH
LFEQMARLSGPGASFATFTSAGAVRRGLAGAGFEVRKAPGHGAKRHISHGWIATPPDAGW
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHCCCCCEECCCCCCC
QAPWYARPEPRWRERSAIVVGGGLAGAASARSLALRGWQVTLIERMPQLASAASGNPQGV
CCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHCCCEEHHHHHHHHHHHHCCCCCCEE
LYTKLSPHLTPLTRLVLSGYAYSLRALRCLLPEDGDWQACGVLQLAHDGKEAEKQQALAE
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHH
LNLPESVMRPLDRDAASELAGTDLPCGGLFFPQGGWVHPPALVRQLADHPNIVIKTGRTA
CCCCHHHHHHCCHHHHHHHCCCCCCCCCEECCCCCCCCCHHHHHHHHCCCCEEEECCCEE
LTLDYDPAQRSWTAGDEQGPLAVASVVVLAGAAETAQFDSTRHLPLKKIRGQITSIPASE
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCHHHHCCHHHCCCCCC
ESSRLRTVLCGEGYISPARGGRHCLGATFKFDTDDLGVNDGEHAENLAMLAELAPSLRAS
CCCCEEEEEECCCCCCCCCCCCEEECEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LDRDAPEHLDGRAAFRCTSPDYLPLVGPAVAARDFVHAYRELARDATLRPATACPWAEGL
CCCCCHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
YVNAAHGSRGLITAPLSGEILASLLEGEPAPLPADLMRAVHPSRFLLRDLIRRKLDPDAL
EEEECCCCCCEEECCCCHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
THSHAQLVWQDGQPMSAVFGDVYFSRASGLEETRHVFLRHNQLEERFAALPPSGSFAIA
CCCCCEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
ETGFGTGLNFLCAWQCFEARAPAGARLHFVSAEKFPLTPSDLAQALALWPELEPWSSQLL
ECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHH
AQYDVLTPGWHRFVLAGGRVTLTLMIGDVLEVLPQLDARVDAWFLDGFAPSKNPDMWQQA
HHHCCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHCCHHCEEEECCCCCCCCHHHHHHH
LFEQMARLSGPGASFATFTSAGAVRRGLAGAGFEVRKAPGHGAKRHISHGWIATPPDAGW
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHCCCCCEECCCCCCC
QAPWYARPEPRWRERSAIVVGGGLAGAASARSLALRGWQVTLIERMPQLASAASGNPQGV
CCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHCCCEEHHHHHHHHHHHHCCCCCCEE
LYTKLSPHLTPLTRLVLSGYAYSLRALRCLLPEDGDWQACGVLQLAHDGKEAEKQQALAE
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHH
LNLPESVMRPLDRDAASELAGTDLPCGGLFFPQGGWVHPPALVRQLADHPNIVIKTGRTA
CCCCHHHHHHCCHHHHHHHCCCCCCCCCEECCCCCCCCCHHHHHHHHCCCCEEEECCCEE
LTLDYDPAQRSWTAGDEQGPLAVASVVVLAGAAETAQFDSTRHLPLKKIRGQITSIPASE
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCHHHHCCHHHCCCCCC
ESSRLRTVLCGEGYISPARGGRHCLGATFKFDTDDLGVNDGEHAENLAMLAELAPSLRAS
CCCCEEEEEECCCCCCCCCCCCEEECEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LDRDAPEHLDGRAAFRCTSPDYLPLVGPAVAARDFVHAYRELARDATLRPATACPWAEGL
CCCCCHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
YVNAAHGSRGLITAPLSGEILASLLEGEPAPLPADLMRAVHPSRFLLRDLIRRKLDPDAL
EEEECCCCCCEEECCCCHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782