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The map label for this gene is nupC [H]

Identifier: 34497386

GI number: 34497386

Start: 2099949

End: 2101139

Strand: Reverse

Name: nupC [H]

Synonym: CV_1931

Alternate gene names: 34497386

Gene position: 2101139-2099949 (Counterclockwise)

Preceding gene: 34497387

Following gene: 34497385

Centisome position: 44.22

GC content: 60.87

Gene sequence:

>1191_bases
ATGCATATCGTCCAATTTCTGATCGGCCTGGTCGTGGTACTGGCCCTCACCCAGCTGGTCGCCCGCGACCGGAAGAACAT
CAAGATCCGCTCCGTGATCCAGCTGCTGGTCATCGAGATCGCCCTGGCGTTCTTCCTGCTCAACACCACCTACGGCCTCG
CCATCGTCGGCAGCATCTCCAGCGTCTTCACCAAGCTGATGGACTTCGCCGGCGAAGGCACCAAGTTCGCCTTCGGCGGC
ATCCAGAACAAGGAAGAGTTCAGCTTCTTCCTGAACGTGCTGATGCCCATCGTCTTCATCTCGGTGCTGATCGGCATCCT
GCAGCACATCCGCGTGCTGCCGGTGGTGATCCGCGCGATCGGCTTCGTGCTGTCCAAGATCAGCGGCATGGGCAAGATCG
AATCGTTCAACGCCATCAGCTCGATGATGGTCGGCCAGTCCGAAAACTTCATCGCCTACAAGAACATCATCAACACCTTC
GATGAGAAGCGCATGTACACGCTGGCCGCCACCGCGATGTCCACCGTGTCGATGTCCATCGTCGGCTCCTACATGCAGAT
GATCGAGCCGCGCTTCGTGGTGACCGCGCTGGTGCTCAACCTGTTCAGCACCTTCGTCGTGCTGTCGCTGATCAACCCCT
ACGACAGCGCGCTGGGCGAGGACGAGCTGATCAGCAACGAAGGCCACCGCGTGTCCTTCTTCGAGATGCTGGGCGAGTAC
ATCCTGACCGGCTTCAAGATCGCCGTGATCGTCGCCGCCATGCTGGTCGGCTTCATCGCCCTGATCGCCTGCGTCAACTT
CATCTTCACCTCCATCTTCGGCATCAACTTCCAGACCATGCTGGGCTACGTGTTCTGGCCGTTCGCCTGGCTGATGGGCG
TGCCGTCCCACGAGGCGCTGACCGCCAGCAGCATCATGGCCACCAAGCTGGTGACCAACGAGTTCGTCGCCATGCTGGAA
CTGAAGAACCAGGCGGCCCACCTGTCTTCCTACACCCGCGGCGTGCTGTCCATCTTCCTGGTGTCCTTCGCCAACTTCAG
CTCCATCGGCATCATCGCCGGCGCGGTGAAAGGCCTGGACGAGGAGCGCGGCAACATGGTGTCCCGCTTCGGCCTGAAGC
TGGTCTACGGCTCCTCGCTGGTGAGCATCCTGTCGGCGCTGATCGCCGGCCTGGTGCTGGCCTCCTACTGA

Upstream 100 bases:

>100_bases
TTTTTATAAAAACCGGCATACGGCCCGCGGCAACAGGCGGGACCGGAACCTTGCTCGATGCCTGCCCCGGCAGGAAGAAA
CCCACAAAGCGACATTCATC

Downstream 100 bases:

>100_bases
GCCGCCCGCTCCTCCGATCAACCGCCCGCCTCCGCGCGGGCGGTTTTGTTTTGCGCAGCGCTGCCGCATAATGGCCGCCT
GACCCACTGGAAATCCGCCA

Product: nucleoside permease

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: Nucleoside-transport system protein nupC [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MHIVQFLIGLVVVLALTQLVARDRKNIKIRSVIQLLVIEIALAFFLLNTTYGLAIVGSISSVFTKLMDFAGEGTKFAFGG
IQNKEEFSFFLNVLMPIVFISVLIGILQHIRVLPVVIRAIGFVLSKISGMGKIESFNAISSMMVGQSENFIAYKNIINTF
DEKRMYTLAATAMSTVSMSIVGSYMQMIEPRFVVTALVLNLFSTFVVLSLINPYDSALGEDELISNEGHRVSFFEMLGEY
ILTGFKIAVIVAAMLVGFIALIACVNFIFTSIFGINFQTMLGYVFWPFAWLMGVPSHEALTASSIMATKLVTNEFVAMLE
LKNQAAHLSSYTRGVLSIFLVSFANFSSIGIIAGAVKGLDEERGNMVSRFGLKLVYGSSLVSILSALIAGLVLASY

Sequences:

>Translated_396_residues
MHIVQFLIGLVVVLALTQLVARDRKNIKIRSVIQLLVIEIALAFFLLNTTYGLAIVGSISSVFTKLMDFAGEGTKFAFGG
IQNKEEFSFFLNVLMPIVFISVLIGILQHIRVLPVVIRAIGFVLSKISGMGKIESFNAISSMMVGQSENFIAYKNIINTF
DEKRMYTLAATAMSTVSMSIVGSYMQMIEPRFVVTALVLNLFSTFVVLSLINPYDSALGEDELISNEGHRVSFFEMLGEY
ILTGFKIAVIVAAMLVGFIALIACVNFIFTSIFGINFQTMLGYVFWPFAWLMGVPSHEALTASSIMATKLVTNEFVAMLE
LKNQAAHLSSYTRGVLSIFLVSFANFSSIGIIAGAVKGLDEERGNMVSRFGLKLVYGSSLVSILSALIAGLVLASY
>Mature_396_residues
MHIVQFLIGLVVVLALTQLVARDRKNIKIRSVIQLLVIEIALAFFLLNTTYGLAIVGSISSVFTKLMDFAGEGTKFAFGG
IQNKEEFSFFLNVLMPIVFISVLIGILQHIRVLPVVIRAIGFVLSKISGMGKIESFNAISSMMVGQSENFIAYKNIINTF
DEKRMYTLAATAMSTVSMSIVGSYMQMIEPRFVVTALVLNLFSTFVVLSLINPYDSALGEDELISNEGHRVSFFEMLGEY
ILTGFKIAVIVAAMLVGFIALIACVNFIFTSIFGINFQTMLGYVFWPFAWLMGVPSHEALTASSIMATKLVTNEFVAMLE
LKNQAAHLSSYTRGVLSIFLVSFANFSSIGIIAGAVKGLDEERGNMVSRFGLKLVYGSSLVSILSALIAGLVLASY

Specific function: Transports nucleosides with a high affinity except guanosine and deoxyguanosine. Driven by a proton motive force [H]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI42542381, Length=412, Percent_Identity=25, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI11545853, Length=410, Percent_Identity=20.9756097560976, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI227116277, Length=398, Percent_Identity=20.6030150753769, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788737, Length=398, Percent_Identity=64.8241206030151, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1788485, Length=410, Percent_Identity=28.2926829268293, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1788488, Length=410, Percent_Identity=29.2682926829268, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17560276, Length=392, Percent_Identity=22.1938775510204, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71991794, Length=391, Percent_Identity=21.9948849104859, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45552519, Length=385, Percent_Identity=23.3766233766234, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45552517, Length=385, Percent_Identity=23.3766233766234, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19921868, Length=385, Percent_Identity=23.3766233766234, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281360430, Length=398, Percent_Identity=21.105527638191, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 43311; Mature: 43311

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIVQFLIGLVVVLALTQLVARDRKNIKIRSVIQLLVIEIALAFFLLNTTYGLAIVGSIS
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVFTKLMDFAGEGTKFAFGGIQNKEEFSFFLNVLMPIVFISVLIGILQHIRVLPVVIRAI
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFVLSKISGMGKIESFNAISSMMVGQSENFIAYKNIINTFDEKRMYTLAATAMSTVSMSI
HHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGSYMQMIEPRFVVTALVLNLFSTFVVLSLINPYDSALGEDELISNEGHRVSFFEMLGEY
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHCCCCCEEHHHHHHHHH
ILTGFKIAVIVAAMLVGFIALIACVNFIFTSIFGINFQTMLGYVFWPFAWLMGVPSHEAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHH
TASSIMATKLVTNEFVAMLELKNQAAHLSSYTRGVLSIFLVSFANFSSIGIIAGAVKGLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCH
EERGNMVSRFGLKLVYGSSLVSILSALIAGLVLASY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHIVQFLIGLVVVLALTQLVARDRKNIKIRSVIQLLVIEIALAFFLLNTTYGLAIVGSIS
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVFTKLMDFAGEGTKFAFGGIQNKEEFSFFLNVLMPIVFISVLIGILQHIRVLPVVIRAI
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFVLSKISGMGKIESFNAISSMMVGQSENFIAYKNIINTFDEKRMYTLAATAMSTVSMSI
HHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGSYMQMIEPRFVVTALVLNLFSTFVVLSLINPYDSALGEDELISNEGHRVSFFEMLGEY
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHCCCCCEEHHHHHHHHH
ILTGFKIAVIVAAMLVGFIALIACVNFIFTSIFGINFQTMLGYVFWPFAWLMGVPSHEAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHH
TASSIMATKLVTNEFVAMLELKNQAAHLSSYTRGVLSIFLVSFANFSSIGIIAGAVKGLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCH
EERGNMVSRFGLKLVYGSSLVSILSALIAGLVLASY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]