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The map label for this gene is sbp [H]

Identifier: 34497287

GI number: 34497287

Start: 1989991

End: 1991028

Strand: Reverse

Name: sbp [H]

Synonym: CV_1832

Alternate gene names: 34497287

Gene position: 1991028-1989991 (Counterclockwise)

Preceding gene: 34497290

Following gene: 34497285

Centisome position: 41.91

GC content: 65.32

Gene sequence:

>1038_bases
GTGCCCTCTATCGAAACATTCACCATCACGGGAAACAAGACCATGCGCCCCCTGCACCGCCTCGCCATCGCCCTGTTGCT
CGCCGGCAGCGCCTCCGCCTTCGCCGAGGTCACCCTGCTCAACGTCTCCTACGACCCGACGCGCGAGCTCTACCAGGACT
ACAACGCCGCCTTCGCCAAATACTGGAAGGCCAAGACCGGCGAGACCGTCGCCGTCAAGCAATCGCACGGCGGTTCCGGC
AAGCAGGCGCGCTCGGTGATAGACGGGCTGGAGGCCGATGTGGTGACGCTGGCGCTGGCCTACGACATCGACGAGATCGC
CAGCCAGGCCAAGACCATCGCGCCCAACTGGCAACAGCGGCTGCCCAATAACGCGTCGCCGTACTCGTCCACCATCGTGT
TCCTGGTACGCAAGGGCAACCCCAAGCACATCAAGGACTGGAACGACCTGGTCAAATCCGGCGTGGAGGTGGTGACGCCG
AACCCGAAAACCGGCGGCGGCGCGCGCTGGAACTACCTGGCGGCCTGGGGCTACGCGCTGAAGCAGCCCGGCGGCAACGA
CGCCAAGGCCAAGGACTTCGTCAAGAAATTGTACGGCAACGTCAAGGTGCTGGACTCGGGCGCGCGCGGCTCGCTGATCA
CCTTCACCCAGCGCGGCATCGGCGACGTGCTGCTGTCCTGGGAGAACGAAGCCTTCCTGGCAACCAAGGAGCTGGGGCCG
GACAAGTTCGACATCGTCACGCCGTCCGTCTCCATCCTGGCCGAACCGCCGGTTAGCGTGGTCGACAAGGTGGCGGACAA
GCGCGGCACCCGCAAGGTGGCCGAAGCCTACCTGAGATACCTGTACAGCCCGGAGGGCCAGGCCCTGGCGGCCAAGCACT
ACTACCGCCCGCGCGATCCGCAGGTGGCGGCCAAATACGCCGGCCAGTTCAGCAAGGTGAAGCTGTTCACCATCGACCAG
ACTTTCGGCGGCTGGCAGAAGGCGCAGAAGACTCACTTCGCCGACGGCGGCGTGTTCGACCAGATCGTCGCGCGCTGA

Upstream 100 bases:

>100_bases
CGATAAGCCTATCTGTTTTTCCGGAATGCCGGCCTTTGCCGTATTTTCGATATCGATTGCAGATTTTCTTGTTTCCCGCC
GCCATCCGGCCCCGCCTACA

Downstream 100 bases:

>100_bases
GGCATGACAGTAACTTCCGGGCTTGAGAAAAGCAGCCACGCATCCGACGCGCCCAGGTCCCTTCTAGCCTGCCGACGGGT
GGCAGGCCCGAGGTCTTAAG

Product: sulfate transport system sulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MPSIETFTITGNKTMRPLHRLAIALLLAGSASAFAEVTLLNVSYDPTRELYQDYNAAFAKYWKAKTGETVAVKQSHGGSG
KQARSVIDGLEADVVTLALAYDIDEIASQAKTIAPNWQQRLPNNASPYSSTIVFLVRKGNPKHIKDWNDLVKSGVEVVTP
NPKTGGGARWNYLAAWGYALKQPGGNDAKAKDFVKKLYGNVKVLDSGARGSLITFTQRGIGDVLLSWENEAFLATKELGP
DKFDIVTPSVSILAEPPVSVVDKVADKRGTRKVAEAYLRYLYSPEGQALAAKHYYRPRDPQVAAKYAGQFSKVKLFTIDQ
TFGGWQKAQKTHFADGGVFDQIVAR

Sequences:

>Translated_345_residues
MPSIETFTITGNKTMRPLHRLAIALLLAGSASAFAEVTLLNVSYDPTRELYQDYNAAFAKYWKAKTGETVAVKQSHGGSG
KQARSVIDGLEADVVTLALAYDIDEIASQAKTIAPNWQQRLPNNASPYSSTIVFLVRKGNPKHIKDWNDLVKSGVEVVTP
NPKTGGGARWNYLAAWGYALKQPGGNDAKAKDFVKKLYGNVKVLDSGARGSLITFTQRGIGDVLLSWENEAFLATKELGP
DKFDIVTPSVSILAEPPVSVVDKVADKRGTRKVAEAYLRYLYSPEGQALAAKHYYRPRDPQVAAKYAGQFSKVKLFTIDQ
TFGGWQKAQKTHFADGGVFDQIVAR
>Mature_344_residues
PSIETFTITGNKTMRPLHRLAIALLLAGSASAFAEVTLLNVSYDPTRELYQDYNAAFAKYWKAKTGETVAVKQSHGGSGK
QARSVIDGLEADVVTLALAYDIDEIASQAKTIAPNWQQRLPNNASPYSSTIVFLVRKGNPKHIKDWNDLVKSGVEVVTPN
PKTGGGARWNYLAAWGYALKQPGGNDAKAKDFVKKLYGNVKVLDSGARGSLITFTQRGIGDVLLSWENEAFLATKELGPD
KFDIVTPSVSILAEPPVSVVDKVADKRGTRKVAEAYLRYLYSPEGQALAAKHYYRPRDPQVAAKYAGQFSKVKLFTIDQT
FGGWQKAQKTHFADGGVFDQIVAR

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=325, Percent_Identity=67.6923076923077, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1788765, Length=307, Percent_Identity=47.2312703583062, Blast_Score=299, Evalue=2e-82,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37745; Mature: 37614

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIETFTITGNKTMRPLHRLAIALLLAGSASAFAEVTLLNVSYDPTRELYQDYNAAFAK
CCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
YWKAKTGETVAVKQSHGGSGKQARSVIDGLEADVVTLALAYDIDEIASQAKTIAPNWQQR
HHHCCCCCEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHCCCCHHHH
LPNNASPYSSTIVFLVRKGNPKHIKDWNDLVKSGVEVVTPNPKTGGGARWNYLAAWGYAL
CCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHH
KQPGGNDAKAKDFVKKLYGNVKVLDSGARGSLITFTQRGIGDVLLSWENEAFLATKELGP
CCCCCCCCHHHHHHHHHHCCEEEEECCCCCCEEEEECCCCCCEEEECCCCEEEEECCCCC
DKFDIVTPSVSILAEPPVSVVDKVADKRGTRKVAEAYLRYLYSPEGQALAAKHYYRPRDP
CCEEEECCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEHHHCCCCCCC
QVAAKYAGQFSKVKLFTIDQTFGGWQKAQKTHFADGGVFDQIVAR
HHHHHHCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCHHHHHHCC
>Mature Secondary Structure 
PSIETFTITGNKTMRPLHRLAIALLLAGSASAFAEVTLLNVSYDPTRELYQDYNAAFAK
CCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
YWKAKTGETVAVKQSHGGSGKQARSVIDGLEADVVTLALAYDIDEIASQAKTIAPNWQQR
HHHCCCCCEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHCCCCHHHH
LPNNASPYSSTIVFLVRKGNPKHIKDWNDLVKSGVEVVTPNPKTGGGARWNYLAAWGYAL
CCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHH
KQPGGNDAKAKDFVKKLYGNVKVLDSGARGSLITFTQRGIGDVLLSWENEAFLATKELGP
CCCCCCCCHHHHHHHHHHCCEEEEECCCCCCEEEEECCCCCCEEEECCCCEEEEECCCCC
DKFDIVTPSVSILAEPPVSVVDKVADKRGTRKVAEAYLRYLYSPEGQALAAKHYYRPRDP
CCEEEECCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEHHHCCCCCCC
QVAAKYAGQFSKVKLFTIDQTFGGWQKAQKTHFADGGVFDQIVAR
HHHHHHCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]