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The map label for this gene is cysU [H]

Identifier: 34497285

GI number: 34497285

Start: 1988584

End: 1989456

Strand: Reverse

Name: cysU [H]

Synonym: CV_1830

Alternate gene names: 34497285

Gene position: 1989456-1988584 (Counterclockwise)

Preceding gene: 34497287

Following gene: 34497284

Centisome position: 41.87

GC content: 70.56

Gene sequence:

>873_bases
TTGGAATCCACGATGTCAGCGACACTCGCTCCGCCACCCCGCCCGCCCCGCGTCCTGCCCGGCTTCAGCCTGTCGCTGGG
CTTCACCGTCAGCTATCTGTCGCTGCTGGTGCTGATTCCGCTGGCCGCGGTGGCCTTGCGCGCCGCCGGCCAGCCGCTGG
AAAGCTTCTGGCAGGCCGCCGCTTCGCCGCGGGTGCTGGCGTCCTACCGGCTCAGCTTCGGCATGGCGCTGGCGGCGGCC
GCCATCAACAGCGTGTTCGGCCTGCTGCTGGCCTGGTCGCTGACCCGCTACCGCTTTCCCGGCAAGCGGCTGGTGGACGC
GCTGGTGGACCTGCCGTTCGCGCTGCCGACAGCGGTGGCCGGTATCGCGCTGACCGCGCTGTACGCCGGCAACGGCTGGC
TGGGCCGATACCTGGAGCCGCTGGGCGTCAAGGTGGCTTTTGGCCCGCTGGGCGTGCTGGTGGCGCTGATCTTCATCGGC
CTGCCCTTCGTGGTGCGCACGGTGCAGCCGGTGCTGGAAGATCTGGAGTCCGAGCTGGAGGAGGCCGCGGCCTGCCTGGG
CGCGCGCCGCTGGCAGATTTTCCGCCACGTGATCCTGCCCACGCTGCAGCCGGCGCTGCTGACCGGCTTCGCGCTGGCCT
TCGCCCGCGCGGTCGGCGAGTACGGCTCGGTGATCTTCATCGCCGGCAACGTGCCCATGGTGTCGGAGATCACCCCGCTG
ATGATCATCAGCAAGCTGGAGCAGTTCGACTACGCCGGCGCCGCCGCCATCGCCAGCGTGATGCTGGCCGCGTCCTTCCT
GCTGTTGCTGGCGATCAACGGCCTGCAGTTCTGGAGCGCGCGCCGACACGGCCGCGCGGGAGGCGCCCGATGA

Upstream 100 bases:

>100_bases
TTCGTCCGTCGCCCACTTTGCATAATATCGAATAAGCATTGAAGAAATACTTATTTTCAGAATAAGACCCTCACGGATAT
CGTTTATTCGGTTATATGAA

Downstream 100 bases:

>100_bases
GCGCCGCCATCACCCATCCGCGCCCGGCGACAGACAAGGCCGCGCAACTGGAATCCCGCGCCGCCACCCGCGAGTCGCGC
GCCGTCAAGTGGACCATCCT

Product: sulfate transport system permease CysU

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MESTMSATLAPPPRPPRVLPGFSLSLGFTVSYLSLLVLIPLAAVALRAAGQPLESFWQAAASPRVLASYRLSFGMALAAA
AINSVFGLLLAWSLTRYRFPGKRLVDALVDLPFALPTAVAGIALTALYAGNGWLGRYLEPLGVKVAFGPLGVLVALIFIG
LPFVVRTVQPVLEDLESELEEAAACLGARRWQIFRHVILPTLQPALLTGFALAFARAVGEYGSVIFIAGNVPMVSEITPL
MIISKLEQFDYAGAAAIASVMLAASFLLLLAINGLQFWSARRHGRAGGAR

Sequences:

>Translated_290_residues
MESTMSATLAPPPRPPRVLPGFSLSLGFTVSYLSLLVLIPLAAVALRAAGQPLESFWQAAASPRVLASYRLSFGMALAAA
AINSVFGLLLAWSLTRYRFPGKRLVDALVDLPFALPTAVAGIALTALYAGNGWLGRYLEPLGVKVAFGPLGVLVALIFIG
LPFVVRTVQPVLEDLESELEEAAACLGARRWQIFRHVILPTLQPALLTGFALAFARAVGEYGSVIFIAGNVPMVSEITPL
MIISKLEQFDYAGAAAIASVMLAASFLLLLAINGLQFWSARRHGRAGGAR
>Mature_290_residues
MESTMSATLAPPPRPPRVLPGFSLSLGFTVSYLSLLVLIPLAAVALRAAGQPLESFWQAAASPRVLASYRLSFGMALAAA
AINSVFGLLLAWSLTRYRFPGKRLVDALVDLPFALPTAVAGIALTALYAGNGWLGRYLEPLGVKVAFGPLGVLVALIFIG
LPFVVRTVQPVLEDLESELEEAAACLGARRWQIFRHVILPTLQPALLTGFALAFARAVGEYGSVIFIAGNVPMVSEITPL
MIISKLEQFDYAGAAAIASVMLAASFLLLLAINGLQFWSARRHGRAGGAR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=269, Percent_Identity=53.5315985130111, Blast_Score=255, Evalue=2e-69,
Organism=Escherichia coli, GI87082099, Length=273, Percent_Identity=34.4322344322344, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1786980, Length=202, Percent_Identity=34.1584158415842, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1786254, Length=204, Percent_Identity=29.9019607843137, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787368, Length=210, Percent_Identity=30.952380952381, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787369, Length=155, Percent_Identity=30.9677419354839, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790464, Length=196, Percent_Identity=32.1428571428571, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30802; Mature: 30802

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTMSATLAPPPRPPRVLPGFSLSLGFTVSYLSLLVLIPLAAVALRAAGQPLESFWQAA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ASPRVLASYRLSFGMALAAAAINSVFGLLLAWSLTRYRFPGKRLVDALVDLPFALPTAVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHH
GIALTALYAGNGWLGRYLEPLGVKVAFGPLGVLVALIFIGLPFVVRTVQPVLEDLESELE
HHHHHHHHCCCCCHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAAACLGARRWQIFRHVILPTLQPALLTGFALAFARAVGEYGSVIFIAGNVPMVSEITPL
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
MIISKLEQFDYAGAAAIASVMLAASFLLLLAINGLQFWSARRHGRAGGAR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MESTMSATLAPPPRPPRVLPGFSLSLGFTVSYLSLLVLIPLAAVALRAAGQPLESFWQAA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ASPRVLASYRLSFGMALAAAAINSVFGLLLAWSLTRYRFPGKRLVDALVDLPFALPTAVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHH
GIALTALYAGNGWLGRYLEPLGVKVAFGPLGVLVALIFIGLPFVVRTVQPVLEDLESELE
HHHHHHHHCCCCCHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAAACLGARRWQIFRHVILPTLQPALLTGFALAFARAVGEYGSVIFIAGNVPMVSEITPL
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
MIISKLEQFDYAGAAAIASVMLAASFLLLLAINGLQFWSARRHGRAGGAR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]