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The map label for this gene is pyrD

Identifier: 34497267

GI number: 34497267

Start: 1969826

End: 1970860

Strand: Reverse

Name: pyrD

Synonym: CV_1812

Alternate gene names: 34497267

Gene position: 1970860-1969826 (Counterclockwise)

Preceding gene: 34497268

Following gene: 34497266

Centisome position: 41.48

GC content: 65.89

Gene sequence:

>1035_bases
ATGCTGTACCCGCTGCTGCGCCCGCTGCTGTTCAAGTTCGACGCGGAAACCGCCCACGAGCACACGCTGAAGATGCTCGA
CCGCGCGCATCGGCTGCATCTGACGCCGCTGGCCGCCTCTCCCGCCGCGCGCCAGCCGGTCCAGGCGATGGGCCTGACTT
TCCCGAACCCGGTGGGACTGGCCGCCGGCCTGGACAAGAACGGCGCCCACATCGACGCGCTGGCCGCGCTGGGCTTCGGC
TTCATCGAAATCGGCACCGTCACCCCCCGCCCTCAGGACGGTAATCCCAAGCCGCGGCTGTTCCGCCTGCCTGAGCACGA
AGCCATCATCAACCGGATGGGTTTCAACAACCATGGCGTGGCCGCGCTGCTGGACAACGTCCGCCGCAGCAAATTCAAGG
GCGTGCTGGGCATCAACATCGGCAAGAACGCGATCACGCCGATAGAAAACGCGGTGGACGACTACCTGGCCTGCCTGGAT
CAGGTCTACGCCGCCGCCAGCTACGTCACCGTCAACATCTCCTCGCCCAACACCAAGAACCTGCGCCAACTGCAGCAAGG
CGACGAATTGGGCCGGCTGCTGGCCGCGCTGAAACAGCGCCAGCTGGCGCTGGCCGATCAGCACGGCCGCTACGTGCCGC
TGGCGGTGAAGATCGCGCCGGACCTGGACGACGAGCAGATCGCGGAAATCGCCCGCCTGCTGACCGGCAACGGCATAGAC
GGCGTCATCGCCACCAACACCACGCTGTCGCGCCGCGAAGTGGCCGGCCATCCGCTGGAAAGCGAGGCGGGCGGCTTGTC
CGGCGCGCCGGTCCGCGCCCGATCCACAGAAGTCATACGCAAACTGCACAAGGAACTTGGCGGCGCCATGCCTATCATAG
GTGTTGGCGGCATCCTTTCCGGCAATGACGCCGTGGAAAAACTGGATGCCGGCGCCAGCCTGGTGCAGCTGTACAGCGGC
CTGATCTATCGCGGCCCTGAACTGGTCGGCGAATGCGCCCGGGCGACGGCGCAATATCTACAAGCTCGCAACTAA

Upstream 100 bases:

>100_bases
CTTCGACGAGGCCTGCCAGATCCTGTAAACAGACGATTGCCGCTGGCCTGATCCCAATGCCCGGTTATAATGCCGGGCAT
TTGTCTGCCCGGAGAATGCC

Downstream 100 bases:

>100_bases
AGTTAAGAATGGAGCAGGAAAAATGATTTCTTCCCTCAACCGCATCACCCTGATCAGCCTTCTGAGCGTCGGCGTGCTCG
CCGGCTGCTCGACGTCCGAC

Product: dihydroorotate dehydrogenase 2

Products: NA

Alternate protein names: DHOdehase; DHOD; DHODase; Dihydroorotate oxidase

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MLYPLLRPLLFKFDAETAHEHTLKMLDRAHRLHLTPLAASPAARQPVQAMGLTFPNPVGLAAGLDKNGAHIDALAALGFG
FIEIGTVTPRPQDGNPKPRLFRLPEHEAIINRMGFNNHGVAALLDNVRRSKFKGVLGINIGKNAITPIENAVDDYLACLD
QVYAAASYVTVNISSPNTKNLRQLQQGDELGRLLAALKQRQLALADQHGRYVPLAVKIAPDLDDEQIAEIARLLTGNGID
GVIATNTTLSRREVAGHPLESEAGGLSGAPVRARSTEVIRKLHKELGGAMPIIGVGGILSGNDAVEKLDAGASLVQLYSG
LIYRGPELVGECARATAQYLQARN

Sequences:

>Translated_344_residues
MLYPLLRPLLFKFDAETAHEHTLKMLDRAHRLHLTPLAASPAARQPVQAMGLTFPNPVGLAAGLDKNGAHIDALAALGFG
FIEIGTVTPRPQDGNPKPRLFRLPEHEAIINRMGFNNHGVAALLDNVRRSKFKGVLGINIGKNAITPIENAVDDYLACLD
QVYAAASYVTVNISSPNTKNLRQLQQGDELGRLLAALKQRQLALADQHGRYVPLAVKIAPDLDDEQIAEIARLLTGNGID
GVIATNTTLSRREVAGHPLESEAGGLSGAPVRARSTEVIRKLHKELGGAMPIIGVGGILSGNDAVEKLDAGASLVQLYSG
LIYRGPELVGECARATAQYLQARN
>Mature_344_residues
MLYPLLRPLLFKFDAETAHEHTLKMLDRAHRLHLTPLAASPAARQPVQAMGLTFPNPVGLAAGLDKNGAHIDALAALGFG
FIEIGTVTPRPQDGNPKPRLFRLPEHEAIINRMGFNNHGVAALLDNVRRSKFKGVLGINIGKNAITPIENAVDDYLACLD
QVYAAASYVTVNISSPNTKNLRQLQQGDELGRLLAALKQRQLALADQHGRYVPLAVKIAPDLDDEQIAEIARLLTGNGID
GVIATNTTLSRREVAGHPLESEAGGLSGAPVRARSTEVIRKLHKELGGAMPIIGVGGILSGNDAVEKLDAGASLVQLYSG
LIYRGPELVGECARATAQYLQARN

Specific function: Pyrimidine biosynthesis; fourth step. [C]

COG id: COG0167

COG function: function code F; Dihydroorotate dehydrogenase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI45006951, Length=324, Percent_Identity=46.6049382716049, Blast_Score=261, Evalue=9e-70,
Organism=Escherichia coli, GI1787177, Length=331, Percent_Identity=56.4954682779456, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17509475, Length=351, Percent_Identity=41.8803418803419, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6322633, Length=308, Percent_Identity=23.7012987012987, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281361352, Length=286, Percent_Identity=45.1048951048951, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI17137316, Length=286, Percent_Identity=45.1048951048951, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRD_CHRVO (Q7NX17)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901482.1
- ProteinModelPortal:   Q7NX17
- SMR:   Q7NX17
- GeneID:   2550125
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1812
- NMPDR:   fig|243365.1.peg.1812
- HOGENOM:   HBG351027
- OMA:   SYVTVNI
- PhylomeDB:   Q7NX17
- ProtClustDB:   PRK05286
- BioCyc:   CVIO243365:CV_1812-MONOMER
- BRENDA:   1.3.3.1
- HAMAP:   MF_00225
- InterPro:   IPR013785
- InterPro:   IPR012135
- InterPro:   IPR005719
- InterPro:   IPR001295
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000164
- TIGRFAMs:   TIGR01036

Pfam domain/function: PF01180 DHO_dh

EC number: =1.3.5.2

Molecular weight: Translated: 36844; Mature: 36844

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS00911 DHODEHASE_1; PS00912 DHODEHASE_2

Important sites: ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYPLLRPLLFKFDAETAHEHTLKMLDRAHRLHLTPLAASPAARQPVQAMGLTFPNPVGL
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCHHCCHHHHCCCCCCCCCH
AAGLDKNGAHIDALAALGFGFIEIGTVTPRPQDGNPKPRLFRLPEHEAIINRMGFNNHGV
HCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCH
AALLDNVRRSKFKGVLGINIGKNAITPIENAVDDYLACLDQVYAAASYVTVNISSPNTKN
HHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHH
LRQLQQGDELGRLLAALKQRQLALADQHGRYVPLAVKIAPDLDDEQIAEIARLLTGNGID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCC
GVIATNTTLSRREVAGHPLESEAGGLSGAPVRARSTEVIRKLHKELGGAMPIIGVGGILS
CEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCEEC
GNDAVEKLDAGASLVQLYSGLIYRGPELVGECARATAQYLQARN
CCHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLYPLLRPLLFKFDAETAHEHTLKMLDRAHRLHLTPLAASPAARQPVQAMGLTFPNPVGL
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCHHCCHHHHCCCCCCCCCH
AAGLDKNGAHIDALAALGFGFIEIGTVTPRPQDGNPKPRLFRLPEHEAIINRMGFNNHGV
HCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCH
AALLDNVRRSKFKGVLGINIGKNAITPIENAVDDYLACLDQVYAAASYVTVNISSPNTKN
HHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHH
LRQLQQGDELGRLLAALKQRQLALADQHGRYVPLAVKIAPDLDDEQIAEIARLLTGNGID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCC
GVIATNTTLSRREVAGHPLESEAGGLSGAPVRARSTEVIRKLHKELGGAMPIIGVGGILS
CEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCEEC
GNDAVEKLDAGASLVQLYSGLIYRGPELVGECARATAQYLQARN
CCHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14500782