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The map label for this gene is glnS

Identifier: 34497197

GI number: 34497197

Start: 1891711

End: 1893393

Strand: Direct

Name: glnS

Synonym: CV_1742

Alternate gene names: 34497197

Gene position: 1891711-1893393 (Clockwise)

Preceding gene: 34497196

Following gene: 34497198

Centisome position: 39.82

GC content: 64.17

Gene sequence:

>1683_bases
ATGAGCACGGAAAACAACGCGCCGGTTGTCAACAACTTCATTCGCAGCATCATCGACGAGGATTTGGCCACCGGCCGCCG
CAGCTCGGTGGTGACCCGCTTCCCGCCCGAGCCGAACGGCTTCGCCCACATCGGCCACGCCAAGGCGATCTGCATCAACT
TCGGCCTGGCCGAGGACTACAACGGCCAGTGCAATCTGCGCATGGACGACACCAATCCGGAAAAGGAATCGGACGAGTTC
GTCGAGGCGTTCAAGCAGGACATCTCCTGGCTGGGCTTCAAGTGGAACGGCGAGGTGCGCTACGCATCCGACTACTTCGA
CCGCCTGTACGACTACGCGGTGGAGTTGATCCAGGCCGGCAAGGCCTACGTGGACGACCTGTCCGCCGAGGAAATGCGCC
AGTACCGCGGCAATCTGACCGAGCCGGGCAAGAACAGCCCCTACCGCGACCGCACGCCGGAAGAGAACCTGGACCTGTTC
ACCCGCATGAAGAACGGCGAGTTCCCGGATGGCAGCAAGACGCTGCGCCTGAAGATCGATATGGCTTCCGGCAACATCAA
TCTGCGCGATCCGGCCATCTACCGTATCCGCCGCGTGCATCACCATCGCACTGGGGACAAGTGGTGCATCTACCCGATGT
ACGACTACACCCACTGCATCTCCGACGCGATCGAGGGCATCACCCATTCGCTGTGCTCGCTGGAGTTCGAGGACCACCGC
CCGCTGTACGACTGGGTGCTGGACAACATCAGCATCGAGCACCATCCGCAGCAGATCGAATTCTCGCGCCTGGAACTGCT
GTACGCGCTGACCTCCAAACGCAAGCTGCAGGCGCTGGTCAACGACGGCGCCGTCACCGGCTGGGACGATCCGCGCATGC
CCACCATCGCCGGCATGCGCCGCCGCGGCTACAGCCCGGCCGGCATCAAGCTGTTCGCCCAGCGCATCGGCGTGTCGAAG
AGCGAAAACATCATCGACATGGCCATCCTGGAAGGCGCGGTGCGCGAGACGCTGGAAAACGATTCGCCGCGCGTGATGGC
CGTGGTCAATCCGCTGAAAGTGACGCTGACCAACTACGACGCCGCCGTCACCGCCAGCCGCAGCGCGCCGTTCCACCCGC
ACCATCCGGAATTCGGCGAGCGCGACGTGCCGATCGCCCGCGAGATCTGGATCGAGCGCGACGACTTCGCCGAAACGCCG
CCGCCGAAGTGGCAGCGCCTGACCGCCGGCGGCGAAGTGCGCCTGCGCTACTCGTACGTGATCAAGTGCGACGAGGTGGT
GAAGGACGCCGCCGACGAGATCGTCGAGCTGAAGTGCTCGATCGACCACGACACGCTGGGCAAGAACCCGGAAGGCCGCA
AGGTCAAGGGCGTGATCCACTGGGTGTCGGCCGAGCATGCGATCCAGGCCGACGTGCGCTGGTACGAGCGCCTGTTCACC
GAGCAGCGTCCGGACGCGGTGCGCGGCGAGGACGGCGAATACGTCGATTTCCGCCAGTTCCTGAACCCGGAGTCGCTGAA
ACTGGTGCCGGCCTATGTCGAGGCCTCGGTGCTGCAGGCGGAGCCGGAGTCGCGTTTCCAGTTCGAGCGTCTGGGCTACT
TCGTCACCGACCGCTACGAGCACCGGAAGGGCGACAAGGCGGTGTTCAACCGCACCGTCGGGCTGAAAGACAGCTGGAAG
TAA

Upstream 100 bases:

>100_bases
AGCGGCTTGGTCGGTGCGCGCGGCGAATCCGGTCATTGGCGAAAATGCTGGAAAAATCGGTTACAATGCCCGATTCGCTA
TCCATACCCACAGCCAGAAC

Downstream 100 bases:

>100_bases
ATCACCGCAGCACTGCCGGATGGCGAGCCCCAGGGTTCGCCATTTTTGCATGCGGAAGCCGTTTCCGCGCGCGAGGCCCG
GCCTACCAGGAGAAAAAATG

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MSTENNAPVVNNFIRSIIDEDLATGRRSSVVTRFPPEPNGFAHIGHAKAICINFGLAEDYNGQCNLRMDDTNPEKESDEF
VEAFKQDISWLGFKWNGEVRYASDYFDRLYDYAVELIQAGKAYVDDLSAEEMRQYRGNLTEPGKNSPYRDRTPEENLDLF
TRMKNGEFPDGSKTLRLKIDMASGNINLRDPAIYRIRRVHHHRTGDKWCIYPMYDYTHCISDAIEGITHSLCSLEFEDHR
PLYDWVLDNISIEHHPQQIEFSRLELLYALTSKRKLQALVNDGAVTGWDDPRMPTIAGMRRRGYSPAGIKLFAQRIGVSK
SENIIDMAILEGAVRETLENDSPRVMAVVNPLKVTLTNYDAAVTASRSAPFHPHHPEFGERDVPIAREIWIERDDFAETP
PPKWQRLTAGGEVRLRYSYVIKCDEVVKDAADEIVELKCSIDHDTLGKNPEGRKVKGVIHWVSAEHAIQADVRWYERLFT
EQRPDAVRGEDGEYVDFRQFLNPESLKLVPAYVEASVLQAEPESRFQFERLGYFVTDRYEHRKGDKAVFNRTVGLKDSWK

Sequences:

>Translated_560_residues
MSTENNAPVVNNFIRSIIDEDLATGRRSSVVTRFPPEPNGFAHIGHAKAICINFGLAEDYNGQCNLRMDDTNPEKESDEF
VEAFKQDISWLGFKWNGEVRYASDYFDRLYDYAVELIQAGKAYVDDLSAEEMRQYRGNLTEPGKNSPYRDRTPEENLDLF
TRMKNGEFPDGSKTLRLKIDMASGNINLRDPAIYRIRRVHHHRTGDKWCIYPMYDYTHCISDAIEGITHSLCSLEFEDHR
PLYDWVLDNISIEHHPQQIEFSRLELLYALTSKRKLQALVNDGAVTGWDDPRMPTIAGMRRRGYSPAGIKLFAQRIGVSK
SENIIDMAILEGAVRETLENDSPRVMAVVNPLKVTLTNYDAAVTASRSAPFHPHHPEFGERDVPIAREIWIERDDFAETP
PPKWQRLTAGGEVRLRYSYVIKCDEVVKDAADEIVELKCSIDHDTLGKNPEGRKVKGVIHWVSAEHAIQADVRWYERLFT
EQRPDAVRGEDGEYVDFRQFLNPESLKLVPAYVEASVLQAEPESRFQFERLGYFVTDRYEHRKGDKAVFNRTVGLKDSWK
>Mature_559_residues
STENNAPVVNNFIRSIIDEDLATGRRSSVVTRFPPEPNGFAHIGHAKAICINFGLAEDYNGQCNLRMDDTNPEKESDEFV
EAFKQDISWLGFKWNGEVRYASDYFDRLYDYAVELIQAGKAYVDDLSAEEMRQYRGNLTEPGKNSPYRDRTPEENLDLFT
RMKNGEFPDGSKTLRLKIDMASGNINLRDPAIYRIRRVHHHRTGDKWCIYPMYDYTHCISDAIEGITHSLCSLEFEDHRP
LYDWVLDNISIEHHPQQIEFSRLELLYALTSKRKLQALVNDGAVTGWDDPRMPTIAGMRRRGYSPAGIKLFAQRIGVSKS
ENIIDMAILEGAVRETLENDSPRVMAVVNPLKVTLTNYDAAVTASRSAPFHPHHPEFGERDVPIAREIWIERDDFAETPP
PKWQRLTAGGEVRLRYSYVIKCDEVVKDAADEIVELKCSIDHDTLGKNPEGRKVKGVIHWVSAEHAIQADVRWYERLFTE
QRPDAVRGEDGEYVDFRQFLNPESLKLVPAYVEASVLQAEPESRFQFERLGYFVTDRYEHRKGDKAVFNRTVGLKDSWK

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI4826960, Length=530, Percent_Identity=46.2264150943396, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI62241042, Length=520, Percent_Identity=33.4615384615385, Blast_Score=259, Evalue=3e-69,
Organism=Escherichia coli, GI1786895, Length=550, Percent_Identity=58.1818181818182, Blast_Score=670, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=122, Percent_Identity=28.6885245901639, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17543332, Length=535, Percent_Identity=42.6168224299065, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17511007, Length=528, Percent_Identity=32.0075757575758, Blast_Score=254, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6324742, Length=570, Percent_Identity=38.7719298245614, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6321192, Length=524, Percent_Identity=35.6870229007634, Blast_Score=297, Evalue=3e-81,
Organism=Drosophila melanogaster, GI17864488, Length=552, Percent_Identity=42.0289855072464, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24649466, Length=519, Percent_Identity=32.1772639691715, Blast_Score=241, Evalue=1e-63,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYQ_CHRVO (Q7NX86)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901412.1
- ProteinModelPortal:   Q7NX86
- SMR:   Q7NX86
- GeneID:   2551030
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1742
- NMPDR:   fig|243365.1.peg.1742
- HOGENOM:   HBG334108
- OMA:   RMPTIAG
- PhylomeDB:   Q7NX86
- ProtClustDB:   PRK05347
- BioCyc:   CVIO243365:CV_1742-MONOMER
- BRENDA:   6.1.1.18
- GO:   GO:0005737
- HAMAP:   MF_00126
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:2.40.240.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119:SF3
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00440

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.18

Molecular weight: Translated: 64373; Mature: 64242

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTENNAPVVNNFIRSIIDEDLATGRRSSVVTRFPPEPNGFAHIGHAKAICINFGLAEDY
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEECCCCEEEEEEECCCCCC
NGQCNLRMDDTNPEKESDEFVEAFKQDISWLGFKWNGEVRYASDYFDRLYDYAVELIQAG
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEEECCCEEEHHHHHHHHHHHHHHHHHHC
KAYVDDLSAEEMRQYRGNLTEPGKNSPYRDRTPEENLDLFTRMKNGEFPDGSKTLRLKID
HHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEEE
MASGNINLRDPAIYRIRRVHHHRTGDKWCIYPMYDYTHCISDAIEGITHSLCSLEFEDHR
EECCCCCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCC
PLYDWVLDNISIEHHPQQIEFSRLELLYALTSKRKLQALVNDGAVTGWDDPRMPTIAGMR
CHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHH
RRGYSPAGIKLFAQRIGVSKSENIIDMAILEGAVRETLENDSPRVMAVVNPLKVTLTNYD
HCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCC
AAVTASRSAPFHPHHPEFGERDVPIAREIWIERDDFAETPPPKWQRLTAGGEVRLRYSYV
EEEEECCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCCCCCCCEEECCCCEEEEEEEEE
IKCDEVVKDAADEIVELKCSIDHDTLGKNPEGRKVKGVIHWVSAEHAIQADVRWYERLFT
EEHHHHHHHHHHHHEEEEEECCHHCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHH
EQRPDAVRGEDGEYVDFRQFLNPESLKLVPAYVEASVLQAEPESRFQFERLGYFVTDRYE
CCCCCCCCCCCCCEEHHHHHCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHCCHHCCCHH
HRKGDKAVFNRTVGLKDSWK
HCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
STENNAPVVNNFIRSIIDEDLATGRRSSVVTRFPPEPNGFAHIGHAKAICINFGLAEDY
CCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEECCCCEEEEEEECCCCCC
NGQCNLRMDDTNPEKESDEFVEAFKQDISWLGFKWNGEVRYASDYFDRLYDYAVELIQAG
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEEECCCEEEHHHHHHHHHHHHHHHHHHC
KAYVDDLSAEEMRQYRGNLTEPGKNSPYRDRTPEENLDLFTRMKNGEFPDGSKTLRLKID
HHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEEE
MASGNINLRDPAIYRIRRVHHHRTGDKWCIYPMYDYTHCISDAIEGITHSLCSLEFEDHR
EECCCCCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCC
PLYDWVLDNISIEHHPQQIEFSRLELLYALTSKRKLQALVNDGAVTGWDDPRMPTIAGMR
CHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHH
RRGYSPAGIKLFAQRIGVSKSENIIDMAILEGAVRETLENDSPRVMAVVNPLKVTLTNYD
HCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCC
AAVTASRSAPFHPHHPEFGERDVPIAREIWIERDDFAETPPPKWQRLTAGGEVRLRYSYV
EEEEECCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCCCCCCCEEECCCCEEEEEEEEE
IKCDEVVKDAADEIVELKCSIDHDTLGKNPEGRKVKGVIHWVSAEHAIQADVRWYERLFT
EEHHHHHHHHHHHHEEEEEECCHHCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHH
EQRPDAVRGEDGEYVDFRQFLNPESLKLVPAYVEASVLQAEPESRFQFERLGYFVTDRYE
CCCCCCCCCCCCCEEHHHHHCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHCCHHCCCHH
HRKGDKAVFNRTVGLKDSWK
HCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782