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The map label for this gene is ushA [H]

Identifier: 34497198

GI number: 34497198

Start: 1893491

End: 1895137

Strand: Direct

Name: ushA [H]

Synonym: CV_1743

Alternate gene names: 34497198

Gene position: 1893491-1895137 (Clockwise)

Preceding gene: 34497197

Following gene: 34497199

Centisome position: 39.85

GC content: 66.06

Gene sequence:

>1647_bases
ATGCGCACCGAGCTCAAATCCATCGCCGTATCCCTGCTGGCCGCCGGCCTGCTGTCCGCCTGCGCCACCCCGCCGGGCAA
GGGCAATTACGAAAGCGGCAAGACCTACAAGATCACCATCCTGCACACCAACGACCACCACGGCCGCTTCTGGCCGAACA
GCGACGGCGAGTACGGCCTCGCCGCGCAGAAGACGGTGGTGGACGCGGTGCGCGCCGAGGTGAAGGCCGACGGCGGCTAC
ATGCTGCTGCTGTCCGGCGGGGACATCAACACCGGGGTGCCGGAATCCGATCTGCAGAATGCCGAGCCGGACTTCCGCGG
CATGGACCGCATCGGCTACGACGCGATGGCGGTCGGCAACCACGAGTTCGACAAGCCGGTGCCGGTGCTGATGCAGCAGC
GCAAGTGGATCAACTTCCCGATGCTGTCCGCCAACATCTACCAGGACGGCAAACGGATGTTCGATCCTTACGCCATGTTC
AACCTGGGCGGCGTCAAGGTGGCGGTGATGGGCCTGACCACCGACGATACCGCCAAGATGGTGAATCCGGCGCAGATCAA
GGGCATCGAATTCAAAAGCCCGATCGCCGAGGCCGCCGCGCTGGTGCCGGAGTTGCGCCAGAAGGCCGACATTGTCATCG
CCGCCACCCATATGGGCCATTACGCCGACGGCAATCACGGCGTCAACGCGCCGGGCGACGTGGAGATGGCGCGCGCGGTG
AAGGGGCTGGACCTGATCGTCGGCGGCCATAGCCAGAATCCGGTGTGCATGAAGGCTGAGAACGTCCGCGACGACGCCTA
CGTGCCGGGCGCGCCTTGCGCGCCGGACCGCCAGAACGGCGCCTGGATCGTGCAGGCGCACGAGTGGGGCAAGTACGTCG
GCCGCGCCGATTTCGAGTTCCGCAACGGCAAGCTCAAGCTGGTCAAATACCAGCTGATTCCGGTCAATCTGAAAAAGACG
GTGAAGACCGCCGACGGCAAGACCGAGAAAGTGCCGTACACCGAGCTGATTCCAGAGGACGGCAAGCTGCGCAGCTTCCT
CAAGGCCTACCAGGACAAGGGCCAGGCGGAGCTGGGCGTCAAAGTGGGCGAGCTGTCGGCCAAGCTGGAGGGCGACCGTT
CCGTGGTGCGCGGCCAGCCCACCAATCTGGGCGTGTTCGTCGGCGAGGCGATGATGGCCAGGACCCAGGCCGACTTCGCG
GTGATCAACTCCGGCGGCATCCGCGACTCGCTGCCGGCCGGCGTGGTCAGCTACAAGGACGTGCTCAAGGTGTTCCCGTT
CGGCAGCACCTTGGCCTACGTCGACATGAGCGGGGCCGAGGCGCTGGATTACCTGAAGGCCGCGGCGCGGATGACGCCGG
GCGCGGGCGCCTTCGCCCAGTTCGCCGGCGTGAAGCTGCGCATCGTCGGCGGCGAGCTGCAGCGGGCGGAGGCGGGCGGC
AAGGCCATCGTGCCGACCGGTCGCTACCGGGTGGCGATCAATAGCTTCATGGCCTCCGGCGGCGATGGTTACCCGAAAAT
GGTCGACCATCCGGGCTATGTGAATACCGGCTTCGTCGACGCCGAAGTGCTCAAGGATTACGTGGCCAGGCATTCGCCTA
TCCAGGCCGCTCGTTACGCGCCGGCCGACGCGGTGGTGCGCCAGTAG

Upstream 100 bases:

>100_bases
TAAATCACCGCAGCACTGCCGGATGGCGAGCCCCAGGGTTCGCCATTTTTGCATGCGGAAGCCGTTTCCGCGCGCGAGGC
CCGGCCTACCAGGAGAAAAA

Downstream 100 bases:

>100_bases
GCGCCGAGTCGGCCGCGGTGTTTGCACGCGGCGCGGAACTAGCCCTTAGTACTGGTGTGGGGTTCCGGAGGGTGGTCTAG
CCTGACTGGGCCGGACGCGG

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic

Products: NA

Alternate protein names: UDP-sugar hydrolase; UDP-sugar diphosphatase; UDP-sugar pyrophosphatase; 5'-nucleotidase; 5'-NT [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MRTELKSIAVSLLAAGLLSACATPPGKGNYESGKTYKITILHTNDHHGRFWPNSDGEYGLAAQKTVVDAVRAEVKADGGY
MLLLSGGDINTGVPESDLQNAEPDFRGMDRIGYDAMAVGNHEFDKPVPVLMQQRKWINFPMLSANIYQDGKRMFDPYAMF
NLGGVKVAVMGLTTDDTAKMVNPAQIKGIEFKSPIAEAAALVPELRQKADIVIAATHMGHYADGNHGVNAPGDVEMARAV
KGLDLIVGGHSQNPVCMKAENVRDDAYVPGAPCAPDRQNGAWIVQAHEWGKYVGRADFEFRNGKLKLVKYQLIPVNLKKT
VKTADGKTEKVPYTELIPEDGKLRSFLKAYQDKGQAELGVKVGELSAKLEGDRSVVRGQPTNLGVFVGEAMMARTQADFA
VINSGGIRDSLPAGVVSYKDVLKVFPFGSTLAYVDMSGAEALDYLKAAARMTPGAGAFAQFAGVKLRIVGGELQRAEAGG
KAIVPTGRYRVAINSFMASGGDGYPKMVDHPGYVNTGFVDAEVLKDYVARHSPIQAARYAPADAVVRQ

Sequences:

>Translated_548_residues
MRTELKSIAVSLLAAGLLSACATPPGKGNYESGKTYKITILHTNDHHGRFWPNSDGEYGLAAQKTVVDAVRAEVKADGGY
MLLLSGGDINTGVPESDLQNAEPDFRGMDRIGYDAMAVGNHEFDKPVPVLMQQRKWINFPMLSANIYQDGKRMFDPYAMF
NLGGVKVAVMGLTTDDTAKMVNPAQIKGIEFKSPIAEAAALVPELRQKADIVIAATHMGHYADGNHGVNAPGDVEMARAV
KGLDLIVGGHSQNPVCMKAENVRDDAYVPGAPCAPDRQNGAWIVQAHEWGKYVGRADFEFRNGKLKLVKYQLIPVNLKKT
VKTADGKTEKVPYTELIPEDGKLRSFLKAYQDKGQAELGVKVGELSAKLEGDRSVVRGQPTNLGVFVGEAMMARTQADFA
VINSGGIRDSLPAGVVSYKDVLKVFPFGSTLAYVDMSGAEALDYLKAAARMTPGAGAFAQFAGVKLRIVGGELQRAEAGG
KAIVPTGRYRVAINSFMASGGDGYPKMVDHPGYVNTGFVDAEVLKDYVARHSPIQAARYAPADAVVRQ
>Mature_548_residues
MRTELKSIAVSLLAAGLLSACATPPGKGNYESGKTYKITILHTNDHHGRFWPNSDGEYGLAAQKTVVDAVRAEVKADGGY
MLLLSGGDINTGVPESDLQNAEPDFRGMDRIGYDAMAVGNHEFDKPVPVLMQQRKWINFPMLSANIYQDGKRMFDPYAMF
NLGGVKVAVMGLTTDDTAKMVNPAQIKGIEFKSPIAEAAALVPELRQKADIVIAATHMGHYADGNHGVNAPGDVEMARAV
KGLDLIVGGHSQNPVCMKAENVRDDAYVPGAPCAPDRQNGAWIVQAHEWGKYVGRADFEFRNGKLKLVKYQLIPVNLKKT
VKTADGKTEKVPYTELIPEDGKLRSFLKAYQDKGQAELGVKVGELSAKLEGDRSVVRGQPTNLGVFVGEAMMARTQADFA
VINSGGIRDSLPAGVVSYKDVLKVFPFGSTLAYVDMSGAEALDYLKAAARMTPGAGAFAQFAGVKLRIVGGELQRAEAGG
KAIVPTGRYRVAINSFMASGGDGYPKMVDHPGYVNTGFVDAEVLKDYVARHSPIQAARYAPADAVVRQ

Specific function: Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm. Note=Exported from the cell, except a small proportion that is internally localized [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=550, Percent_Identity=25.0909090909091, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI1786687, Length=554, Percent_Identity=58.3032490974729, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI1790658, Length=548, Percent_Identity=23.3576642335766, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28573524, Length=561, Percent_Identity=25.4901960784314, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922446, Length=523, Percent_Identity=24.8565965583174, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24654424, Length=523, Percent_Identity=24.8565965583174, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922444, Length=527, Percent_Identity=25.426944971537, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161076508, Length=468, Percent_Identity=24.7863247863248, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19921980, Length=468, Percent_Identity=24.7863247863248, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24652512, Length=468, Percent_Identity=24.7863247863248, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI221329836, Length=559, Percent_Identity=22.1824686940966, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24641187, Length=558, Percent_Identity=22.4014336917563, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.6.1.45; =3.1.3.5 [H]

Molecular weight: Translated: 58897; Mature: 58897

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTELKSIAVSLLAAGLLSACATPPGKGNYESGKTYKITILHTNDHHGRFWPNSDGEYGL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCC
AAQKTVVDAVRAEVKADGGYMLLLSGGDINTGVPESDLQNAEPDFRGMDRIGYDAMAVGN
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCHHCCCCEEEECC
HEFDKPVPVLMQQRKWINFPMLSANIYQDGKRMFDPYAMFNLGGVKVAVMGLTTDDTAKM
CCCCCCHHHHHHHCCCCCCCEEECHHHHCHHHHCCCHHEEECCCEEEEEEEECCCCHHHC
VNPAQIKGIEFKSPIAEAAALVPELRQKADIVIAATHMGHYADGNHGVNAPGDVEMARAV
CCCHHCCCEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHH
KGLDLIVGGHSQNPVCMKAENVRDDAYVPGAPCAPDRQNGAWIVQAHEWGKYVGRADFEF
CCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHCCCCCEEE
RNGKLKLVKYQLIPVNLKKTVKTADGKTEKVPYTELIPEDGKLRSFLKAYQDKGQAELGV
ECCEEEEEEEEEEEECHHHHHCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCEEECE
KVGELSAKLEGDRSVVRGQPTNLGVFVGEAMMARTQADFAVINSGGIRDSLPAGVVSYKD
EEEEEHEEECCCCHHHCCCCCCCEEEHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHH
VLKVFPFGSTLAYVDMSGAEALDYLKAAARMTPGAGAFAQFAGVKLRIVGGELQRAEAGG
HHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECCCHHHHCCCC
KAIVPTGRYRVAINSFMASGGDGYPKMVDHPGYVNTGFVDAEVLKDYVARHSPIQAARYA
CEECCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHCC
PADAVVRQ
CHHHHHCC
>Mature Secondary Structure
MRTELKSIAVSLLAAGLLSACATPPGKGNYESGKTYKITILHTNDHHGRFWPNSDGEYGL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCC
AAQKTVVDAVRAEVKADGGYMLLLSGGDINTGVPESDLQNAEPDFRGMDRIGYDAMAVGN
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCHHCCCCEEEECC
HEFDKPVPVLMQQRKWINFPMLSANIYQDGKRMFDPYAMFNLGGVKVAVMGLTTDDTAKM
CCCCCCHHHHHHHCCCCCCCEEECHHHHCHHHHCCCHHEEECCCEEEEEEEECCCCHHHC
VNPAQIKGIEFKSPIAEAAALVPELRQKADIVIAATHMGHYADGNHGVNAPGDVEMARAV
CCCHHCCCEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHH
KGLDLIVGGHSQNPVCMKAENVRDDAYVPGAPCAPDRQNGAWIVQAHEWGKYVGRADFEF
CCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHCCCCCEEE
RNGKLKLVKYQLIPVNLKKTVKTADGKTEKVPYTELIPEDGKLRSFLKAYQDKGQAELGV
ECCEEEEEEEEEEEECHHHHHCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCEEECE
KVGELSAKLEGDRSVVRGQPTNLGVFVGEAMMARTQADFAVINSGGIRDSLPAGVVSYKD
EEEEEHEEECCCCHHHCCCCCCCEEEHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHH
VLKVFPFGSTLAYVDMSGAEALDYLKAAARMTPGAGAFAQFAGVKLRIVGGELQRAEAGG
HHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECCCHHHHCCCC
KAIVPTGRYRVAINSFMASGGDGYPKMVDHPGYVNTGFVDAEVLKDYVARHSPIQAARYA
CEECCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHCC
PADAVVRQ
CHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3012467; 9278503; 9298646; 10331872; 11491293; 11491294 [H]