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The map label for this gene is kbl [C]

Identifier: 34497105

GI number: 34497105

Start: 1787071

End: 1788270

Strand: Direct

Name: kbl [C]

Synonym: CV_1650

Alternate gene names: 34497105

Gene position: 1787071-1788270 (Clockwise)

Preceding gene: 34497104

Following gene: 34497106

Centisome position: 37.61

GC content: 66.92

Gene sequence:

>1200_bases
ATGAACCACACCTATCTCGCTCACCTGCAGGCTACGCTGGCCCAGATCCGGGCCGACGGTTTCGAGAAGCCGGAGCGCGT
GATCGCCACGCCGCAAAGCGCCGACATCGCGCTGGCCGGCGGCGAGCATGTGCTGAACTTCTGCGCCAACAACTACCTGG
GCCTGGCCGACGACAAGCGCCTGGTGCTGGCCGCCAAGGCCGGCATGGACCAGTACGGCTACGGCTGCGCGTCGGTGCGC
TTCATCTGCGGCACCCAGTCCATCCACAAGCAGCTGGAAAAATCGATTTCCGAGTTCCTCGGCGCCGACGACACCATCCT
GTACTCCAGCTGTTTCGACGCCAACGGCGGCGTGTTCGAAACCCTGCTGACCGAAGAGGACGCGGTGATCTCGGACGAGC
TGAACCACGCGTCCATCATCGACGGCGTGCGCCTGTCCAAGGCCAAGCGCTTCCGCTACAAAAACAACGACATGGCCGAT
CTGGAGGCGCAGCTGGTCGCCGCCGAACAGGCCGGCGCCCGCTTCAAGCTGATCGTCACCGACGGCGTGTTCTCCATGGA
CGGCATCATCGCCGATCTGAAGGGCATCTGCGACCTGGCCGAGCGCCACGACGCGCTGGTGATGGTGGACGACTCGCACG
CGGTGGGCTTCATCGGCGAAACCGGCGCCGGCACGCCGGAATACTGCGGCGTCTCCGACCGCGTCGACATCTACACCGGC
ACGCTGGGCAAGGCGCTGGGCGGCGCGTCCGGCGGCTACGTCGCCGCGCACCAACCCATCGTCGACCTGCTGCGCCAGCG
CTCGCGCCCCTATCTGTTCTCCAACACGCTGGCGCCGGCCATCGCCGCCGCCAGCGTGGAAGTGCTGAACATCCTGAAGG
GCGGCGAGGGCGCGGAGCTGCGCGCCAAGGTGCGCCGCAACGCCGAGCTGTTCCGCAAGGAAATGTCCGCCGCCGGCTTC
ACCCTGGTGCCGGGCGAACACCCGATCATCCCGGTGATGCTGGGCGATGCCCGCCTGGCAGGCGAGATGGCCGCCAAGCT
GCTGGCCGAAGGCGTGTACGTGATCGGCTTCTCCTACCCGGTGGTGCCCAAGGGCAAGGCGCGCATCCGCACCCAGATGT
CCGCCGCGCACAGCGAGGAGCAAGTGAAGAAGGCTGTTGCCGCCTTCATCAAGGTCGGCCGCGAGCTGGGCGTAATCTGA

Upstream 100 bases:

>100_bases
TAGAATGGCGGCAGTTTCCGGCGCGCAGCCGCCAAGCGCGGCGCGCGGTGCCTGCGGACGCGGGTTCGATTCCCGCGTCC
CGCCTATCCAAAGGACTGCC

Downstream 100 bases:

>100_bases
TTTGACCGCATGACAGGCCGCGCGGCGTCCGCGCGGCTTCAAGGATAGAGACATGAAGGCGCTTGCCAAGCTGAAGGCCG
CTCCCGGCCTGGAAATGACC

Product: 2-amino-3-ketobutyrate coenzyme A ligase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MNHTYLAHLQATLAQIRADGFEKPERVIATPQSADIALAGGEHVLNFCANNYLGLADDKRLVLAAKAGMDQYGYGCASVR
FICGTQSIHKQLEKSISEFLGADDTILYSSCFDANGGVFETLLTEEDAVISDELNHASIIDGVRLSKAKRFRYKNNDMAD
LEAQLVAAEQAGARFKLIVTDGVFSMDGIIADLKGICDLAERHDALVMVDDSHAVGFIGETGAGTPEYCGVSDRVDIYTG
TLGKALGGASGGYVAAHQPIVDLLRQRSRPYLFSNTLAPAIAAASVEVLNILKGGEGAELRAKVRRNAELFRKEMSAAGF
TLVPGEHPIIPVMLGDARLAGEMAAKLLAEGVYVIGFSYPVVPKGKARIRTQMSAAHSEEQVKKAVAAFIKVGRELGVI

Sequences:

>Translated_399_residues
MNHTYLAHLQATLAQIRADGFEKPERVIATPQSADIALAGGEHVLNFCANNYLGLADDKRLVLAAKAGMDQYGYGCASVR
FICGTQSIHKQLEKSISEFLGADDTILYSSCFDANGGVFETLLTEEDAVISDELNHASIIDGVRLSKAKRFRYKNNDMAD
LEAQLVAAEQAGARFKLIVTDGVFSMDGIIADLKGICDLAERHDALVMVDDSHAVGFIGETGAGTPEYCGVSDRVDIYTG
TLGKALGGASGGYVAAHQPIVDLLRQRSRPYLFSNTLAPAIAAASVEVLNILKGGEGAELRAKVRRNAELFRKEMSAAGF
TLVPGEHPIIPVMLGDARLAGEMAAKLLAEGVYVIGFSYPVVPKGKARIRTQMSAAHSEEQVKKAVAAFIKVGRELGVI
>Mature_399_residues
MNHTYLAHLQATLAQIRADGFEKPERVIATPQSADIALAGGEHVLNFCANNYLGLADDKRLVLAAKAGMDQYGYGCASVR
FICGTQSIHKQLEKSISEFLGADDTILYSSCFDANGGVFETLLTEEDAVISDELNHASIIDGVRLSKAKRFRYKNNDMAD
LEAQLVAAEQAGARFKLIVTDGVFSMDGIIADLKGICDLAERHDALVMVDDSHAVGFIGETGAGTPEYCGVSDRVDIYTG
TLGKALGGASGGYVAAHQPIVDLLRQRSRPYLFSNTLAPAIAAASVEVLNILKGGEGAELRAKVRRNAELFRKEMSAAGF
TLVPGEHPIIPVMLGDARLAGEMAAKLLAEGVYVIGFSYPVVPKGKARIRTQMSAAHSEEQVKKAVAAFIKVGRELGVI

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=396, Percent_Identity=55.5555555555556, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI284448556, Length=420, Percent_Identity=52.3809523809524, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI83977440, Length=358, Percent_Identity=35.195530726257, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI83977442, Length=358, Percent_Identity=35.195530726257, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI83977444, Length=358, Percent_Identity=35.195530726257, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI4502025, Length=365, Percent_Identity=32.3287671232877, Blast_Score=204, Evalue=8e-53,
Organism=Homo sapiens, GI40316939, Length=365, Percent_Identity=32.3287671232877, Blast_Score=204, Evalue=8e-53,
Organism=Homo sapiens, GI4758668, Length=364, Percent_Identity=30.7692307692308, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI119220554, Length=367, Percent_Identity=31.0626702997275, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI5454084, Length=390, Percent_Identity=26.4102564102564, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1790046, Length=399, Percent_Identity=65.4135338345865, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI1786993, Length=348, Percent_Identity=33.0459770114943, Blast_Score=187, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI71994529, Length=395, Percent_Identity=54.1772151898734, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI32566772, Length=371, Percent_Identity=30.4582210242588, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17560914, Length=358, Percent_Identity=31.5642458100559, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17560912, Length=358, Percent_Identity=31.5642458100559, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI71982617, Length=269, Percent_Identity=28.996282527881, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71982625, Length=269, Percent_Identity=28.996282527881, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320438, Length=375, Percent_Identity=31.7333333333333, Blast_Score=192, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6320267, Length=368, Percent_Identity=31.25, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6323954, Length=302, Percent_Identity=27.4834437086093, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24662918, Length=392, Percent_Identity=57.6530612244898, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI17137420, Length=349, Percent_Identity=31.5186246418338, Blast_Score=191, Evalue=9e-49,
Organism=Drosophila melanogaster, GI17136286, Length=375, Percent_Identity=30.6666666666667, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24653280, Length=347, Percent_Identity=25.6484149855908, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24653276, Length=347, Percent_Identity=25.6484149855908, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24653278, Length=347, Percent_Identity=25.6484149855908, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011282
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 42632; Mature: 42632

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHTYLAHLQATLAQIRADGFEKPERVIATPQSADIALAGGEHVLNFCANNYLGLADDKR
CCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEECCHHHHHHHHCCCCCCCCCCE
LVLAAKAGMDQYGYGCASVRFICGTQSIHKQLEKSISEFLGADDTILYSSCFDANGGVFE
EEEEEECCCCHHCCCHHHEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEH
TLLTEEDAVISDELNHASIIDGVRLSKAKRFRYKNNDMADLEAQLVAAEQAGARFKLIVT
HHHCCCCCHHHCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEE
DGVFSMDGIIADLKGICDLAERHDALVMVDDSHAVGFIGETGAGTPEYCGVSDRVDIYTG
CCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCEEEEEC
TLGKALGGASGGYVAAHQPIVDLLRQRSRPYLFSNTLAPAIAAASVEVLNILKGGEGAEL
CHHHHHCCCCCCEEEECCHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCHHH
RAKVRRNAELFRKEMSAAGFTLVPGEHPIIPVMLGDARLAGEMAAKLLAEGVYVIGFSYP
HHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEECCC
VVPKGKARIRTQMSAAHSEEQVKKAVAAFIKVGRELGVI
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNHTYLAHLQATLAQIRADGFEKPERVIATPQSADIALAGGEHVLNFCANNYLGLADDKR
CCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEECCHHHHHHHHCCCCCCCCCCE
LVLAAKAGMDQYGYGCASVRFICGTQSIHKQLEKSISEFLGADDTILYSSCFDANGGVFE
EEEEEECCCCHHCCCHHHEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEH
TLLTEEDAVISDELNHASIIDGVRLSKAKRFRYKNNDMADLEAQLVAAEQAGARFKLIVT
HHHCCCCCHHHCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEE
DGVFSMDGIIADLKGICDLAERHDALVMVDDSHAVGFIGETGAGTPEYCGVSDRVDIYTG
CCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCEEEEEC
TLGKALGGASGGYVAAHQPIVDLLRQRSRPYLFSNTLAPAIAAASVEVLNILKGGEGAEL
CHHHHHCCCCCCEEEECCHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCHHH
RAKVRRNAELFRKEMSAAGFTLVPGEHPIIPVMLGDARLAGEMAAKLLAEGVYVIGFSYP
HHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEECCC
VVPKGKARIRTQMSAAHSEEQVKKAVAAFIKVGRELGVI
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]