| Definition | Chromobacterium violaceum ATCC 12472 chromosome, complete genome. |
|---|---|
| Accession | NC_005085 |
| Length | 4,751,080 |
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The map label for this gene is nadE [H]
Identifier: 34497071
GI number: 34497071
Start: 1743269
End: 1744861
Strand: Reverse
Name: nadE [H]
Synonym: CV_1616
Alternate gene names: 34497071
Gene position: 1744861-1743269 (Counterclockwise)
Preceding gene: 34497077
Following gene: 34497070
Centisome position: 36.73
GC content: 66.98
Gene sequence:
>1593_bases ATGCGTATCGCTCTCGCCCAGTTCAACCCCGTGGTCGGCGACATCGTCGGCAATGCCCAAAAAATCGTCGACCTCGCCAA CGCGGCCCTGGCCCAGGGCGCGGACATCCTGCTGACGCCGGAGCTGGCGCTGACCGGCTACAGTCCGGAAGACCTGCTGC TGCGCGACAGCTTCTACCGCGAAGTGGCGCGCGGCCTGGACATCATCGAGCAGCTGGACGGCATCACCGTCATCGTCGGC CATCCGGCCAGGATAGGCAACGAGCGCTTCAACGCCGCCACCGTGCTGCGCGACGGCAACCGGCTGGGCCAGTATCACAA GATGCTGCTGCCCAATTATGAAGTGTTCGACGAATGCCGCTACTTCACGCCGGGCGCCGCGCCGCTGGTGTTCGAGCAGA ACGGCGTCAAGGTGGGCGTGCTGATCTGCGAGGACGCCTGGCAGCTGGAGCCGGCGGCCGAAGCCGCCGACGCCGGCGCC GAGCTGCTGCTGAGCCTGAACGCCTCGCCGTTCCACCGCGACAAGATCGAGACCCGCCACCAAGTGATGCGCTACCGCGT GGAGGAGACCGGCCTGCCGCTGGCCTACGCCAACCTCGTCGGCGGCCAGGACGAACTGGTGTTCGACGGCGGCTCTTTCG CCTTGGACAAGGCCGGCAACGTGGCGGCACAGGCGGCGGCCTACGACGACGAGCTGCTGCTGGTGGATTTCGCCTACGGC GACTTCCAGCCCGGCCGGCAAGCCGCGCTGCCCGGAGCGCAGGAAAGCGTCTACCGCGCGCTGGTGGTCGGCGTGCGCGA CTACATAGGCAAGAACGGCTTCCCCGGCGCGCTCTTGGGCCTGTCCGGCGGGATAGACTCGGCGCTGACGCTGGCTGTGG CGGTGGACGCGCTGGGCGCCGACAAGGTGCACGCCGTGATGATGCCGTCGCGCTACACCGCCGACATTTCGGTGACCGAC AGCCGCGACATGATCGGCCGCCTGGGCGTCAAGTACGACGAGATCGAGATCTGGCCGATGTACGAAAGCTTCATGGCCGC GCTGGCCCCGTCGTTCGCCGGCCTGGAGATGGACACCACCGAGGAGAACCTGCAGGCGCGCATCCGCGGCACGCTCTTGA TGGCGCTGTCCAACAAGAGCGGCAAGCTGGTGCTGACCACCGGCAACAAATCCGAGATGACCACCGGCTACTGCACGCTG TACGGCGACATGGCCGGCGGCTTCGCGGTGCTGAAGGACGTGGCCAAGACCCTGGTGTTCGAGCTCTGCCGCTGGCGCAA CACCGTGTCCGACATCATTCCGGAGCGCATCATCACCCGGCCGCCATCGGCCGAGCTGCGGCCAGACCAGAAGGATCAGG ACAGCCTGCCGCCTTACGAGGTGCTGGATGCCATCATGGCGCGTTACGTCGAGGACAACCAGTCGGCCGCCGACATCATC GCCGCGGGCTTTGCCGAAGCCGACGTCAACCGCGTGGTGCGGCTGCTGAAAATCAACGAATACAAGCGCCGCCAGGCGCC GGTGGGCCCGCGCGTCACCCAGCGCGGCTTCGGCAAGGACTGGCGCTACCCGATCACCAACAAGTTCAGCTGA
Upstream 100 bases:
>100_bases GCGGCCACGCCGTCATACAGTTGCAGCTGCAATGCTTTTCCTTTCCGAGAGCCTGTTCAGGTAAAATTGAACGATCAAGA ATCATCGGTGCCCTCTCAAG
Downstream 100 bases:
>100_bases GCCCTGCCCGGGCGGCCGCCGGCCGTCCCGCGGGGACAAGCCAAAGCCATGGCCATCCCGGCTGCGATATAATCCCGGCA TACCCAGACGATCGGCCAAG
Product: NAD synthetase
Products: NA
Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]
Number of amino acids: Translated: 530; Mature: 530
Protein sequence:
>530_residues MRIALAQFNPVVGDIVGNAQKIVDLANAALAQGADILLTPELALTGYSPEDLLLRDSFYREVARGLDIIEQLDGITVIVG HPARIGNERFNAATVLRDGNRLGQYHKMLLPNYEVFDECRYFTPGAAPLVFEQNGVKVGVLICEDAWQLEPAAEAADAGA ELLLSLNASPFHRDKIETRHQVMRYRVEETGLPLAYANLVGGQDELVFDGGSFALDKAGNVAAQAAAYDDELLLVDFAYG DFQPGRQAALPGAQESVYRALVVGVRDYIGKNGFPGALLGLSGGIDSALTLAVAVDALGADKVHAVMMPSRYTADISVTD SRDMIGRLGVKYDEIEIWPMYESFMAALAPSFAGLEMDTTEENLQARIRGTLLMALSNKSGKLVLTTGNKSEMTTGYCTL YGDMAGGFAVLKDVAKTLVFELCRWRNTVSDIIPERIITRPPSAELRPDQKDQDSLPPYEVLDAIMARYVEDNQSAADII AAGFAEADVNRVVRLLKINEYKRRQAPVGPRVTQRGFGKDWRYPITNKFS
Sequences:
>Translated_530_residues MRIALAQFNPVVGDIVGNAQKIVDLANAALAQGADILLTPELALTGYSPEDLLLRDSFYREVARGLDIIEQLDGITVIVG HPARIGNERFNAATVLRDGNRLGQYHKMLLPNYEVFDECRYFTPGAAPLVFEQNGVKVGVLICEDAWQLEPAAEAADAGA ELLLSLNASPFHRDKIETRHQVMRYRVEETGLPLAYANLVGGQDELVFDGGSFALDKAGNVAAQAAAYDDELLLVDFAYG DFQPGRQAALPGAQESVYRALVVGVRDYIGKNGFPGALLGLSGGIDSALTLAVAVDALGADKVHAVMMPSRYTADISVTD SRDMIGRLGVKYDEIEIWPMYESFMAALAPSFAGLEMDTTEENLQARIRGTLLMALSNKSGKLVLTTGNKSEMTTGYCTL YGDMAGGFAVLKDVAKTLVFELCRWRNTVSDIIPERIITRPPSAELRPDQKDQDSLPPYEVLDAIMARYVEDNQSAADII AAGFAEADVNRVVRLLKINEYKRRQAPVGPRVTQRGFGKDWRYPITNKFS >Mature_530_residues MRIALAQFNPVVGDIVGNAQKIVDLANAALAQGADILLTPELALTGYSPEDLLLRDSFYREVARGLDIIEQLDGITVIVG HPARIGNERFNAATVLRDGNRLGQYHKMLLPNYEVFDECRYFTPGAAPLVFEQNGVKVGVLICEDAWQLEPAAEAADAGA ELLLSLNASPFHRDKIETRHQVMRYRVEETGLPLAYANLVGGQDELVFDGGSFALDKAGNVAAQAAAYDDELLLVDFAYG DFQPGRQAALPGAQESVYRALVVGVRDYIGKNGFPGALLGLSGGIDSALTLAVAVDALGADKVHAVMMPSRYTADISVTD SRDMIGRLGVKYDEIEIWPMYESFMAALAPSFAGLEMDTTEENLQARIRGTLLMALSNKSGKLVLTTGNKSEMTTGYCTL YGDMAGGFAVLKDVAKTLVFELCRWRNTVSDIIPERIITRPPSAELRPDQKDQDSLPPYEVLDAIMARYVEDNQSAADII AAGFAEADVNRVVRLLKINEYKRRQAPVGPRVTQRGFGKDWRYPITNKFS
Specific function: Can use both glutamine or ammonia as a nitrogen source [H]
COG id: COG0171
COG function: function code H; NAD synthase
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 CN hydrolase domain [H]
Homologues:
Organism=Caenorhabditis elegans, GI17538756, Length=261, Percent_Identity=25.2873563218391, Blast_Score=69, Evalue=5e-12,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014445 - InterPro: IPR022310 - InterPro: IPR003694 - InterPro: IPR000132 - InterPro: IPR003010 - InterPro: IPR014729 [H]
Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]
EC number: =6.3.5.1 [H]
Molecular weight: Translated: 57850; Mature: 57850
Theoretical pI: Translated: 4.61; Mature: 4.61
Prosite motif: PS50263 CN_HYDROLASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRIALAQFNPVVGDIVGNAQKIVDLANAALAQGADILLTPELALTGYSPEDLLLRDSFYR CEEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCEEEECCCCHHHHHHHHHHH EVARGLDIIEQLDGITVIVGHPARIGNERFNAATVLRDGNRLGQYHKMLLPNYEVFDECR HHHHHHHHHHHCCCEEEEECCCHHCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHCC YFTPGAAPLVFEQNGVKVGVLICEDAWQLEPAAEAADAGAELLLSLNASPFHRDKIETRH CCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEECCCCCCHHHHHHHHH QVMRYRVEETGLPLAYANLVGGQDELVFDGGSFALDKAGNVAAQAAAYDDELLLVDFAYG HHHHHHHHHCCCCEEEHHHCCCCCCEEEECCCEEECCCCCHHHHHHCCCCCEEEEEEECC DFQPGRQAALPGAQESVYRALVVGVRDYIGKNGFPGALLGLSGGIDSALTLAVAVDALGA CCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCC DKVHAVMMPSRYTADISVTDSRDMIGRLGVKYDEIEIWPMYESFMAALAPSFAGLEMDTT CCEEEEECCCCEEEEEEECCCHHHHHHCCCEECEEEEEECHHHHHHHHCCCCCCCCCCCC EENLQARIRGTLLMALSNKSGKLVLTTGNKSEMTTGYCTLYGDMAGGFAVLKDVAKTLVF HHHHHHHHHEEEEEEEECCCCEEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHH ELCRWRNTVSDIIPERIITRPPSAELRPDQKDQDSLPPYEVLDAIMARYVEDNQSAADII HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHH AAGFAEADVNRVVRLLKINEYKRRQAPVGPRVTQRGFGKDWRYPITNKFS HHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCC >Mature Secondary Structure MRIALAQFNPVVGDIVGNAQKIVDLANAALAQGADILLTPELALTGYSPEDLLLRDSFYR CEEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCEEEECCCCHHHHHHHHHHH EVARGLDIIEQLDGITVIVGHPARIGNERFNAATVLRDGNRLGQYHKMLLPNYEVFDECR HHHHHHHHHHHCCCEEEEECCCHHCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHCC YFTPGAAPLVFEQNGVKVGVLICEDAWQLEPAAEAADAGAELLLSLNASPFHRDKIETRH CCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEECCCCCCHHHHHHHHH QVMRYRVEETGLPLAYANLVGGQDELVFDGGSFALDKAGNVAAQAAAYDDELLLVDFAYG HHHHHHHHHCCCCEEEHHHCCCCCCEEEECCCEEECCCCCHHHHHHCCCCCEEEEEEECC DFQPGRQAALPGAQESVYRALVVGVRDYIGKNGFPGALLGLSGGIDSALTLAVAVDALGA CCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCC DKVHAVMMPSRYTADISVTDSRDMIGRLGVKYDEIEIWPMYESFMAALAPSFAGLEMDTT CCEEEEECCCCEEEEEEECCCHHHHHHCCCEECEEEEEECHHHHHHHHCCCCCCCCCCCC EENLQARIRGTLLMALSNKSGKLVLTTGNKSEMTTGYCTLYGDMAGGFAVLKDVAKTLVF HHHHHHHHHEEEEEEEECCCCEEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHH ELCRWRNTVSDIIPERIITRPPSAELRPDQKDQDSLPPYEVLDAIMARYVEDNQSAADII HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHH AAGFAEADVNRVVRLLKINEYKRRQAPVGPRVTQRGFGKDWRYPITNKFS HHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10910347 [H]