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The map label for this gene is pleD [H]

Identifier: 34497042

GI number: 34497042

Start: 1710731

End: 1711774

Strand: Reverse

Name: pleD [H]

Synonym: CV_1587

Alternate gene names: 34497042

Gene position: 1711774-1710731 (Counterclockwise)

Preceding gene: 34497043

Following gene: 34497038

Centisome position: 36.03

GC content: 66.67

Gene sequence:

>1044_bases
TTGTCGCGGCGCGACTTCACCCGCTATGTCCGGGACAAGGCCTGGCTCGCCATCCCCGGCTGGCTGTTCGTCGCGGCGCT
GACCCTGCTGGCGCTGCGCAAACACCTGTCCAACCTGCGCCAGCACCAGCAGCTGCTGCAAGGCAACGAGCGCATACGCG
ACAGCGAAGAACAGGCCAGGCTAATCCTGGACACCGCGCCGCTGGCGCTGCTGCTGGTCAACCCCCAGACCGCCCGCATC
CGCCACGCCAACTCGCTGGCGCGGGAAATGCTGGGCCTGCCGCCGCGCGATCCGGAACAGGCCGCCGGCAGCGAGCTGAA
CCTGCCGCTGAAGCTGCAGCCGGTGGAGCAATGGCTGAGCGAAGGCGAAGAGGTGCTGCAACAGGAAGTGGAGCTGGAGC
TGGAGGACAAGAGCAGGCTGTGGGCGATCGTGTCGCTGCGCCGGCTAGGGCACGACAGCCAGCCGTCGGCGCTGATCGCG
CTGTACGACATCTCCGGCCGCAAGCTGCTGGAACAGCAACTGCACGAAAGCAACCAGCAGCTGGCCGAAATGGCGGTCAC
CGACCCGCTGACCCGGCTATACAACCGCCGCCACGCCGACATGGTGCTGCGCGACGAAATCAGCCGCTGCGAGCGCTACG
GCCAGACCATGGCCATCGCGGTGTTCGACATCGACCATTTCAAGCGCTTCAACGACCAATACGGCCACCAGGCCGGCGAC
GACGTGCTGGTGGCGGTCGCCAACGCGCTGCGCGACACCACCCGCAATACCGACATCAACGCGCGCATCGGCGGCGAGGA
GTTCATGGTGATCTTCCCCTACACGCGGCTGCGCGATGCCCACAAGGTGATGAGCCGGCTGCAGATGGCGCTGACCCACA
CCACCTTCCCCTTCGTCGAACAGCGCATCACCTTCAGCGGCGGCGTCACCGACTGGCGGCCGGGAGACAGCCCGGCGCTG
ATGATGTCCCGCGCCGACAAGCTGTTGTACGACGCCAAGCAGGCGGGCCGCAACGTGGTGCTGTCGGATCAGGAAGAAGC
GTGA

Upstream 100 bases:

>100_bases
GGGGCAGATGCTTTTCGACGCGCCGTCAAGCTTCGACGGCGGCGAGCGGCTGTACAGCATGCGCCAGACCGGCAGCTACC
CGTTCCAGGTCGCCGTCGGC

Downstream 100 bases:

>100_bases
GAGGACAGGTTAGCCGCCCCAGGCGCCAGGCCGCGCGGCGGCATCCCAGCCAAGCCTCCCCGCCATCGCCTTGCCGCGGC
TTTGCGCCGATGCGCGACGT

Product: sensory box/GGDEF family protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MSRRDFTRYVRDKAWLAIPGWLFVAALTLLALRKHLSNLRQHQQLLQGNERIRDSEEQARLILDTAPLALLLVNPQTARI
RHANSLAREMLGLPPRDPEQAAGSELNLPLKLQPVEQWLSEGEEVLQQEVELELEDKSRLWAIVSLRRLGHDSQPSALIA
LYDISGRKLLEQQLHESNQQLAEMAVTDPLTRLYNRRHADMVLRDEISRCERYGQTMAIAVFDIDHFKRFNDQYGHQAGD
DVLVAVANALRDTTRNTDINARIGGEEFMVIFPYTRLRDAHKVMSRLQMALTHTTFPFVEQRITFSGGVTDWRPGDSPAL
MMSRADKLLYDAKQAGRNVVLSDQEEA

Sequences:

>Translated_347_residues
MSRRDFTRYVRDKAWLAIPGWLFVAALTLLALRKHLSNLRQHQQLLQGNERIRDSEEQARLILDTAPLALLLVNPQTARI
RHANSLAREMLGLPPRDPEQAAGSELNLPLKLQPVEQWLSEGEEVLQQEVELELEDKSRLWAIVSLRRLGHDSQPSALIA
LYDISGRKLLEQQLHESNQQLAEMAVTDPLTRLYNRRHADMVLRDEISRCERYGQTMAIAVFDIDHFKRFNDQYGHQAGD
DVLVAVANALRDTTRNTDINARIGGEEFMVIFPYTRLRDAHKVMSRLQMALTHTTFPFVEQRITFSGGVTDWRPGDSPAL
MMSRADKLLYDAKQAGRNVVLSDQEEA
>Mature_346_residues
SRRDFTRYVRDKAWLAIPGWLFVAALTLLALRKHLSNLRQHQQLLQGNERIRDSEEQARLILDTAPLALLLVNPQTARIR
HANSLAREMLGLPPRDPEQAAGSELNLPLKLQPVEQWLSEGEEVLQQEVELELEDKSRLWAIVSLRRLGHDSQPSALIAL
YDISGRKLLEQQLHESNQQLAEMAVTDPLTRLYNRRHADMVLRDEISRCERYGQTMAIAVFDIDHFKRFNDQYGHQAGDD
VLVAVANALRDTTRNTDINARIGGEEFMVIFPYTRLRDAHKVMSRLQMALTHTTFPFVEQRITFSGGVTDWRPGDSPALM
MSRADKLLYDAKQAGRNVVLSDQEEA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=168, Percent_Identity=37.5, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87082007, Length=160, Percent_Identity=38.75, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI87081881, Length=185, Percent_Identity=32.4324324324324, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=31.0126582278481, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788381, Length=308, Percent_Identity=26.6233766233766, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787262, Length=159, Percent_Identity=32.0754716981132, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788085, Length=180, Percent_Identity=32.2222222222222, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081974, Length=156, Percent_Identity=28.2051282051282, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081977, Length=133, Percent_Identity=35.3383458646617, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787802, Length=167, Percent_Identity=31.1377245508982, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787541, Length=179, Percent_Identity=28.4916201117318, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39694; Mature: 39563

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRDFTRYVRDKAWLAIPGWLFVAALTLLALRKHLSNLRQHQQLLQGNERIRDSEEQAR
CCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHH
LILDTAPLALLLVNPQTARIRHANSLAREMLGLPPRDPEQAAGSELNLPLKLQPVEQWLS
EEEECCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEECHHHHHHH
EGEEVLQQEVELELEDKSRLWAIVSLRRLGHDSQPSALIALYDISGRKLLEQQLHESNQQ
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHH
LAEMAVTDPLTRLYNRRHADMVLRDEISRCERYGQTMAIAVFDIDHFKRFNDQYGHQAGD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHCCCCCC
DVLVAVANALRDTTRNTDINARIGGEEFMVIFPYTRLRDAHKVMSRLQMALTHTTFPFVE
HHHHHHHHHHHHHCCCCCCEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRITFSGGVTDWRPGDSPALMMSRADKLLYDAKQAGRNVVLSDQEEA
HHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCC
>Mature Secondary Structure 
SRRDFTRYVRDKAWLAIPGWLFVAALTLLALRKHLSNLRQHQQLLQGNERIRDSEEQAR
CCHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHH
LILDTAPLALLLVNPQTARIRHANSLAREMLGLPPRDPEQAAGSELNLPLKLQPVEQWLS
EEEECCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEECHHHHHHH
EGEEVLQQEVELELEDKSRLWAIVSLRRLGHDSQPSALIALYDISGRKLLEQQLHESNQQ
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHH
LAEMAVTDPLTRLYNRRHADMVLRDEISRCERYGQTMAIAVFDIDHFKRFNDQYGHQAGD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHCCCCCC
DVLVAVANALRDTTRNTDINARIGGEEFMVIFPYTRLRDAHKVMSRLQMALTHTTFPFVE
HHHHHHHHHHHHHCCCCCCEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRITFSGGVTDWRPGDSPALMMSRADKLLYDAKQAGRNVVLSDQEEA
HHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA