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The map label for this gene is gacS [H]

Identifier: 34497036

GI number: 34497036

Start: 1703179

End: 1705173

Strand: Direct

Name: gacS [H]

Synonym: CV_1581

Alternate gene names: 34497036

Gene position: 1703179-1705173 (Clockwise)

Preceding gene: 34497035

Following gene: 34497037

Centisome position: 35.85

GC content: 67.22

Gene sequence:

>1995_bases
CTGGAAGCGCGCAGCCAGGCGCTGCAGCAGGCCAATGTCCAGCTGACCGAGGCGCGCGACGCCGCCGAGGCCGCCAACCG
GGCCAAGAGCGAGTTTCTAGCGGTGATGAGCCACGAAATCCGCACGCCGCTCAACGGCGTGATCGGCATGACCGACCTGC
TGCAAAGAACCCGGCTGGACGATCGGCAGCGCCATTACGTTAGCGTGGTCCATCGTTCCGGCGAGGATTTGCTGGCCATC
ATCAGCGACATCCTGGACTTCTCCAAGATAGAAGCCGGCAAGTTCGAGCTGGACCGGCAGCCGTTTTGCCTCAATCTGCT
GGTCGAGGAAATCGTCGAGCGCCTGGCGCCGGTGGCCTACGGCAAAGGGCTGGAGCTGATGTGCGCGCTGCCGCTGATGC
CGGTGGAGGTGGTGGGCGACGGCAAGCGCCTGGGCCAGGTATTGACCAACCTGGTGGGCAACGCGATCAAATTCACCGAG
TCCGGCCATGTTTTGGCGAAGGTCGACCGGGCGGGCGAGTCGGACGCCGGCGTGACCTGCCGGATCAGCGTCAAGGATAC
CGGCATCGGCATCGCGCCCGATCAGCAGAAAAGGCTGTTCAAGCCATTCTCCCAAGCGGACAGTTCGACCACTCGGCGCT
TTGGCGGCACCGGCCTGGGCCTGGTGATTTCGCAGCGGCTGGTCGGCATGATGGGCGGGCGGATCGAACTGGAAAGCGAA
CCCGGCTCAGGTTCGGAATTCCACTTCACGCTGACGCTGCCGCGCGCGTCGGACAGCCAGCTGTGCCCGCCTGCGGAGGA
TGTGGCGCGCATGAGGGTGCTGGTGCTGGACGACAATGTCACCAATCTGGAGATACTGGAACAGCAGCTGGCGTCCTGGC
ATTGCCGCGTCGCCGCCTTTCACCGGCCGCGGGAGGCGCTGGACTGGCTGACGGCGGGAAACGAAGTGGATCTGATCATC
TCCGACATGATGATGCCGGAGATGGATGGCCTGATGTTCCTGGGCGAGGCGAGGGAACGCCTGGGCGAAGCCGCGCCGCC
CGCCATCATTCTCAGCTCCGCCGCCGACGATTTTCAGAAGCTGACCGGCGCCTCGCGGCTGGCGCGGCAGGTGCTGACCA
AGCCGATCCGCCGGTCTGATCTGTACAACGCGCTTCTGCAACTGGGCTCCGCCGCCGCCAAGGTGGAGCCTGCGCCGGAC
GGACACGACCAGCCGAGGCTCTCAGGCCGGGTGCTGTTGGTGGAGGACAACCTGGTCAACCAGGAGCTGGCGACAGCGAT
GTTGAGAAACCTGGGGTGCGAGGTGGAACTGGCGCGCAATGGCGAGGAAGCGCTGCGCTTGTGCGAGGCCGGGGGCTTCG
ACCTGGCGCTGATGGATTGCGAAATGCCAGTGATGGACGGCTGGCAGGCCACCGGCGCGATCCGGGCGCGCGAGCGGCCC
GGCGGCGCGCGCCTGACCATCGTCGCGCTGACCGCCAATGCGGGACTGGGCGAGCGGGAGCGCTGCCTGCAGGCCGGCAT
GGACGATTACCTGTCCAAACCCTTCACGCAGAATGAACTGGGAGCGGTGCTGTCGCGCTGGCTGTCGCCGGGGGAGGAGG
CGGAAGCGAGCGCGGAGCCGGCCGGAATCGCGGCGCGAGACGACGGAGAATGGGTGGACGCCGGGGCGCTGGACGCGATC
CGCGCCATTCAGCAGGATCTGCTGCCGCGCATGCTGGCGGCGTGGATGAGCGAGGGACCGATGCTGTTGGCCGGCATTCG
CGAGGCCATCGACGCCGTCGACGGCCCGGCGCTGTTCCGCGCGGCCCATGCGCTGAAAAACTGCGCCGGCAGCGTGGGCG
CGCAGCCCTTGGCTTCGCTGTGCATGGAGCTGGAGCGGCTGGGCAAGGGCGAGGATCTGGCCGGCGCCGCGAACCTGTTG
CCGGAGCTGGATCAGGCTTTCGGCTGTTCGATGGCGGCGCTGAAGGCGGTGGACGAGGGCAGCGGCCTGGCCTGA

Upstream 100 bases:

>100_bases
CGGCCTGGGCGCGGTGGATTACCTGATGAAGCCGCTGGACGACAACCTGCTGCTCAACCGCGTCCGGCTTTACCAGAAGC
TGCACGAGCGCGAGACGGCG

Downstream 100 bases:

>100_bases
CGGTGGCGGGTGGGCGAAGTTGCAACAGGCCTGGCGCGGCAGGGCGGCCGTACTCGTTCTCCTGTCCCGCATGGGATGTG
CGGCGGCGCGGCTTGAAGGG

Product: sensor/response hybrid

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MEARSQALQQANVQLTEARDAAEAANRAKSEFLAVMSHEIRTPLNGVIGMTDLLQRTRLDDRQRHYVSVVHRSGEDLLAI
ISDILDFSKIEAGKFELDRQPFCLNLLVEEIVERLAPVAYGKGLELMCALPLMPVEVVGDGKRLGQVLTNLVGNAIKFTE
SGHVLAKVDRAGESDAGVTCRISVKDTGIGIAPDQQKRLFKPFSQADSSTTRRFGGTGLGLVISQRLVGMMGGRIELESE
PGSGSEFHFTLTLPRASDSQLCPPAEDVARMRVLVLDDNVTNLEILEQQLASWHCRVAAFHRPREALDWLTAGNEVDLII
SDMMMPEMDGLMFLGEARERLGEAAPPAIILSSAADDFQKLTGASRLARQVLTKPIRRSDLYNALLQLGSAAAKVEPAPD
GHDQPRLSGRVLLVEDNLVNQELATAMLRNLGCEVELARNGEEALRLCEAGGFDLALMDCEMPVMDGWQATGAIRARERP
GGARLTIVALTANAGLGERERCLQAGMDDYLSKPFTQNELGAVLSRWLSPGEEAEASAEPAGIAARDDGEWVDAGALDAI
RAIQQDLLPRMLAAWMSEGPMLLAGIREAIDAVDGPALFRAAHALKNCAGSVGAQPLASLCMELERLGKGEDLAGAANLL
PELDQAFGCSMAALKAVDEGSGLA

Sequences:

>Translated_664_residues
MEARSQALQQANVQLTEARDAAEAANRAKSEFLAVMSHEIRTPLNGVIGMTDLLQRTRLDDRQRHYVSVVHRSGEDLLAI
ISDILDFSKIEAGKFELDRQPFCLNLLVEEIVERLAPVAYGKGLELMCALPLMPVEVVGDGKRLGQVLTNLVGNAIKFTE
SGHVLAKVDRAGESDAGVTCRISVKDTGIGIAPDQQKRLFKPFSQADSSTTRRFGGTGLGLVISQRLVGMMGGRIELESE
PGSGSEFHFTLTLPRASDSQLCPPAEDVARMRVLVLDDNVTNLEILEQQLASWHCRVAAFHRPREALDWLTAGNEVDLII
SDMMMPEMDGLMFLGEARERLGEAAPPAIILSSAADDFQKLTGASRLARQVLTKPIRRSDLYNALLQLGSAAAKVEPAPD
GHDQPRLSGRVLLVEDNLVNQELATAMLRNLGCEVELARNGEEALRLCEAGGFDLALMDCEMPVMDGWQATGAIRARERP
GGARLTIVALTANAGLGERERCLQAGMDDYLSKPFTQNELGAVLSRWLSPGEEAEASAEPAGIAARDDGEWVDAGALDAI
RAIQQDLLPRMLAAWMSEGPMLLAGIREAIDAVDGPALFRAAHALKNCAGSVGAQPLASLCMELERLGKGEDLAGAANLL
PELDQAFGCSMAALKAVDEGSGLA
>Mature_664_residues
MEARSQALQQANVQLTEARDAAEAANRAKSEFLAVMSHEIRTPLNGVIGMTDLLQRTRLDDRQRHYVSVVHRSGEDLLAI
ISDILDFSKIEAGKFELDRQPFCLNLLVEEIVERLAPVAYGKGLELMCALPLMPVEVVGDGKRLGQVLTNLVGNAIKFTE
SGHVLAKVDRAGESDAGVTCRISVKDTGIGIAPDQQKRLFKPFSQADSSTTRRFGGTGLGLVISQRLVGMMGGRIELESE
PGSGSEFHFTLTLPRASDSQLCPPAEDVARMRVLVLDDNVTNLEILEQQLASWHCRVAAFHRPREALDWLTAGNEVDLII
SDMMMPEMDGLMFLGEARERLGEAAPPAIILSSAADDFQKLTGASRLARQVLTKPIRRSDLYNALLQLGSAAAKVEPAPD
GHDQPRLSGRVLLVEDNLVNQELATAMLRNLGCEVELARNGEEALRLCEAGGFDLALMDCEMPVMDGWQATGAIRARERP
GGARLTIVALTANAGLGERERCLQAGMDDYLSKPFTQNELGAVLSRWLSPGEEAEASAEPAGIAARDDGEWVDAGALDAI
RAIQQDLLPRMLAAWMSEGPMLLAGIREAIDAVDGPALFRAAHALKNCAGSVGAQPLASLCMELERLGKGEDLAGAANLL
PELDQAFGCSMAALKAVDEGSGLA

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=645, Percent_Identity=35.1937984496124, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI145693157, Length=518, Percent_Identity=34.3629343629344, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI48994928, Length=366, Percent_Identity=34.9726775956284, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87081816, Length=389, Percent_Identity=33.1619537275064, Blast_Score=179, Evalue=6e-46,
Organism=Escherichia coli, GI1788713, Length=402, Percent_Identity=32.5870646766169, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1788549, Length=227, Percent_Identity=30.8370044052863, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1786600, Length=241, Percent_Identity=28.6307053941909, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1790346, Length=257, Percent_Identity=28.4046692607004, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788393, Length=246, Percent_Identity=30.8943089430894, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1790436, Length=221, Percent_Identity=33.0316742081448, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786912, Length=254, Percent_Identity=27.1653543307087, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1786783, Length=256, Percent_Identity=27.34375, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1789808, Length=226, Percent_Identity=28.7610619469027, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI87082128, Length=236, Percent_Identity=27.5423728813559, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI87082012, Length=135, Percent_Identity=31.8518518518519, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1790300, Length=227, Percent_Identity=28.1938325991189, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787894, Length=253, Percent_Identity=24.901185770751, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786784, Length=122, Percent_Identity=33.6065573770492, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=371, Percent_Identity=30.4582210242588, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322000, Length=121, Percent_Identity=39.6694214876033, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323034, Length=183, Percent_Identity=29.5081967213115, Blast_Score=85, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 71410; Mature: 71410

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50894 HPT ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEARSQALQQANVQLTEARDAAEAANRAKSEFLAVMSHEIRTPLNGVIGMTDLLQRTRLD
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
DRQRHYVSVVHRSGEDLLAIISDILDFSKIEAGKFELDRQPFCLNLLVEEIVERLAPVAY
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHC
GKGLELMCALPLMPVEVVGDGKRLGQVLTNLVGNAIKFTESGHVLAKVDRAGESDAGVTC
CCCCCEEEECCCCCEEECCCHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCCEEE
RISVKDTGIGIAPDQQKRLFKPFSQADSSTTRRFGGTGLGLVISQRLVGMMGGRIELESE
EEEEECCCCCCCCCHHHHHHCCHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCEEEEECC
PGSGSEFHFTLTLPRASDSQLCPPAEDVARMRVLVLDDNVTNLEILEQQLASWHCRVAAF
CCCCCEEEEEEEECCCCCCCCCCCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRPREALDWLTAGNEVDLIISDMMMPEMDGLMFLGEARERLGEAAPPAIILSSAADDFQK
CCCHHHHHHHHCCCCEEEHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEECCCHHHHHH
LTGASRLARQVLTKPIRRSDLYNALLQLGSAAAKVEPAPDGHDQPRLSGRVLLVEDNLVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEEECCHHH
QELATAMLRNLGCEVELARNGEEALRLCEAGGFDLALMDCEMPVMDGWQATGAIRARERP
HHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEECCCC
GGARLTIVALTANAGLGERERCLQAGMDDYLSKPFTQNELGAVLSRWLSPGEEAEASAEP
CCCEEEEEEEECCCCCCHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
AGIAARDDGEWVDAGALDAIRAIQQDLLPRMLAAWMSEGPMLLAGIREAIDAVDGPALFR
CCEEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHH
AAHALKNCAGSVGAQPLASLCMELERLGKGEDLAGAANLLPELDQAFGCSMAALKAVDEG
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCC
SGLA
CCCC
>Mature Secondary Structure
MEARSQALQQANVQLTEARDAAEAANRAKSEFLAVMSHEIRTPLNGVIGMTDLLQRTRLD
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
DRQRHYVSVVHRSGEDLLAIISDILDFSKIEAGKFELDRQPFCLNLLVEEIVERLAPVAY
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHC
GKGLELMCALPLMPVEVVGDGKRLGQVLTNLVGNAIKFTESGHVLAKVDRAGESDAGVTC
CCCCCEEEECCCCCEEECCCHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCCEEE
RISVKDTGIGIAPDQQKRLFKPFSQADSSTTRRFGGTGLGLVISQRLVGMMGGRIELESE
EEEEECCCCCCCCCHHHHHHCCHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCEEEEECC
PGSGSEFHFTLTLPRASDSQLCPPAEDVARMRVLVLDDNVTNLEILEQQLASWHCRVAAF
CCCCCEEEEEEEECCCCCCCCCCCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRPREALDWLTAGNEVDLIISDMMMPEMDGLMFLGEARERLGEAAPPAIILSSAADDFQK
CCCHHHHHHHHCCCCEEEHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEECCCHHHHHH
LTGASRLARQVLTKPIRRSDLYNALLQLGSAAAKVEPAPDGHDQPRLSGRVLLVEDNLVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEEECCHHH
QELATAMLRNLGCEVELARNGEEALRLCEAGGFDLALMDCEMPVMDGWQATGAIRARERP
HHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEECCCC
GGARLTIVALTANAGLGERERCLQAGMDDYLSKPFTQNELGAVLSRWLSPGEEAEASAEP
CCCEEEEEEEECCCCCCHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
AGIAARDDGEWVDAGALDAIRAIQQDLLPRMLAAWMSEGPMLLAGIREAIDAVDGPALFR
CCEEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHH
AAHALKNCAGSVGAQPLASLCMELERLGKGEDLAGAANLLPELDQAFGCSMAALKAVDEG
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCC
SGLA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]