BacMap

Definition	Chromobacterium violaceum ATCC 12472 chromosome, complete genome.
Accession	NC_005085
Length	4,751,080

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The map label for this gene is paaF [C]

Identifier: 34497008

GI number: 34497008

Start: 1666719

End: 1667501

Strand: Direct

Name: paaF [C]

Synonym: CV_1553

Alternate gene names: 34497008

Gene position: 1666719-1667501 (Clockwise)

Preceding gene: 34497007

Following gene: 34497009

Centisome position: 35.08

GC content: 69.48

Gene sequence:

>783_bases
ATGCTGGAACGGGATGTGGTGAGGAGCAAGGCGGAGGACGGCATCGCGCGGCTGGAGTTGCATCGGCCGGATTGCCTGAA
TGCGATGAACCGCCAGCTGCTGCGGCAACTGCTGGCCGCGCTGGAGTGGGCGGCGGCCAACGACGCGGTGAGGGCGGTGC
TGATCACCGGCCATGGCCGGGTGTTTTCCGCTGGAGCCGACATCCGTTACCTGAACCGGGCGCCGGCGGCCGAGGTGCGC
GAACTGGCCAGGCTGGCGGTGGCGGTCACCGGCCGGATCGAGGCGCTGGGCAAGCCGGTTCTGGCCGCGTTGAACGGCGA
CGCGTTGGGGGGCGGCCTGGAGATCGCCGAGGCCTGCACGCTGCGGGTGGCGGCCTCGCACGCCCGTTTCGGCCATCCGG
AAGTGAAGATAGGCGCGGTGGCCGGTTTCGGCGGCACCACCCGGCTGCCCAGATTGATCGGCAAGGGCAGGGCGGCGGAG
ATGCTGCTGACCGGCAGGCTGATCGACGCCGACGAGGCCTGCCGGCTGGGTCTGGTCAATCGGGTTGTGCCCGCCGACGA
TTTGATCGCCGAGTCCGAGGCGCTGTTGAGCGAGGTCCTGGCCCAGTCGCCGCTGGCGGTGAGGCTGAGCTGGGAAGCCA
TGCATCGCGGCCTGTCTTTGTCCGAGGACGAGTCGGCCCGGCTCGGCGCCGATTACTTCGGCCTGGCGGCGCAAAGCGAG
GATTTCCGCATCGGTACCCGCGCCTTCCTGGACAAGCGGGCGCCCAAGTTCAAGGGGAGATGA

Upstream 100 bases:

>100_bases
TTGCGTGACGCGGAGGGCGCGCTTGCCCGATGCGAACGCGTTCGGCCGAATCTCACAATCTGATGCCGCGTACCGACGGC
GCGGCGGGCACGGAGGCGGG

Downstream 100 bases:

>100_bases
AAGGAGGCGGCGATGAAACCGCGCATCACCTTGCTGACGCTGGGCGTCGACGATCTGGAACGCGCGCTGGCGTTCTATCG
CGACGGATTGGGACTGCCGA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLERDVVRSKAEDGIARLELHRPDCLNAMNRQLLRQLLAALEWAAANDAVRAVLITGHGRVFSAGADIRYLNRAPAAEVR
ELARLAVAVTGRIEALGKPVLAALNGDALGGGLEIAEACTLRVAASHARFGHPEVKIGAVAGFGGTTRLPRLIGKGRAAE
MLLTGRLIDADEACRLGLVNRVVPADDLIAESEALLSEVLAQSPLAVRLSWEAMHRGLSLSEDESARLGADYFGLAAQSE
DFRIGTRAFLDKRAPKFKGR

Sequences:

>Translated_260_residues
MLERDVVRSKAEDGIARLELHRPDCLNAMNRQLLRQLLAALEWAAANDAVRAVLITGHGRVFSAGADIRYLNRAPAAEVR
ELARLAVAVTGRIEALGKPVLAALNGDALGGGLEIAEACTLRVAASHARFGHPEVKIGAVAGFGGTTRLPRLIGKGRAAE
MLLTGRLIDADEACRLGLVNRVVPADDLIAESEALLSEVLAQSPLAVRLSWEAMHRGLSLSEDESARLGADYFGLAAQSE
DFRIGTRAFLDKRAPKFKGR
>Mature_260_residues
MLERDVVRSKAEDGIARLELHRPDCLNAMNRQLLRQLLAALEWAAANDAVRAVLITGHGRVFSAGADIRYLNRAPAAEVR
ELARLAVAVTGRIEALGKPVLAALNGDALGGGLEIAEACTLRVAASHARFGHPEVKIGAVAGFGGTTRLPRLIGKGRAAE
MLLTGRLIDADEACRLGLVNRVVPADDLIAESEALLSEVLAQSPLAVRLSWEAMHRGLSLSEDESARLGADYFGLAAQSE
DFRIGTRAFLDKRAPKFKGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=32.6612903225806, Blast_Score=141, Evalue=5e-34,
Organism=Homo sapiens, GI20127408, Length=188, Percent_Identity=36.7021276595745, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI68989263, Length=200, Percent_Identity=36.5, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI70995211, Length=237, Percent_Identity=30.8016877637131, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI4502327, Length=251, Percent_Identity=33.0677290836653, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI45643119, Length=210, Percent_Identity=29.0476190476191, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI260275230, Length=210, Percent_Identity=29.0476190476191, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI260274832, Length=210, Percent_Identity=29.0476190476191, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI25453481, Length=210, Percent_Identity=30.952380952381, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI51538573, Length=210, Percent_Identity=30.952380952381, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI4757966, Length=210, Percent_Identity=30.952380952381, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI51538581, Length=210, Percent_Identity=30.952380952381, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI221136756, Length=212, Percent_Identity=31.1320754716981, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI221136753, Length=212, Percent_Identity=31.1320754716981, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI221307494, Length=212, Percent_Identity=31.1320754716981, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4757968, Length=185, Percent_Identity=33.5135135135135, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI49355787, Length=185, Percent_Identity=33.5135135135135, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=28.6852589641434, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI150378541, Length=254, Percent_Identity=29.9212598425197, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI116235487, Length=184, Percent_Identity=32.0652173913043, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI37594471, Length=184, Percent_Identity=28.804347826087, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI37594469, Length=184, Percent_Identity=28.804347826087, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI213417737, Length=209, Percent_Identity=28.2296650717703, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI157694516, Length=209, Percent_Identity=28.2296650717703, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI261878539, Length=101, Percent_Identity=40.5940594059406, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1787659, Length=243, Percent_Identity=39.0946502057613, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=32.6923076923077, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=31.0344827586207, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790281, Length=235, Percent_Identity=32.3404255319149, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI221142681, Length=251, Percent_Identity=33.0677290836653, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1788682, Length=202, Percent_Identity=39.1089108910891, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87082183, Length=262, Percent_Identity=26.3358778625954, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=36.5461847389558, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=34.8, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17540714, Length=207, Percent_Identity=32.3671497584541, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=259, Percent_Identity=31.2741312741313, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17558304, Length=223, Percent_Identity=32.2869955156951, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=262, Percent_Identity=26.7175572519084, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17534483, Length=243, Percent_Identity=26.7489711934156, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17508951, Length=223, Percent_Identity=30.9417040358744, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=223, Percent_Identity=30.9417040358744, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=223, Percent_Identity=30.9417040358744, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=259, Percent_Identity=26.6409266409266, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=255, Percent_Identity=25.0980392156863, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=248, Percent_Identity=24.1935483870968, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=213, Percent_Identity=23.943661971831, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=191, Percent_Identity=25.6544502617801, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20129971, Length=252, Percent_Identity=35.3174603174603, Blast_Score=154, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24653477, Length=252, Percent_Identity=35.3174603174603, Blast_Score=154, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=31.8897637795276, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI19922422, Length=256, Percent_Identity=26.953125, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19920382, Length=258, Percent_Identity=29.0697674418605, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI19921000, Length=182, Percent_Identity=37.3626373626374, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24583077, Length=182, Percent_Identity=37.3626373626374, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24583079, Length=182, Percent_Identity=37.3626373626374, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21357171, Length=176, Percent_Identity=32.9545454545455, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24650670, Length=193, Percent_Identity=32.1243523316062, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571730, Length=138, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28571729, Length=138, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921018, Length=233, Percent_Identity=28.3261802575107, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24654903, Length=172, Percent_Identity=26.7441860465116, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583165, Length=210, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27813; Mature: 27813

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERDVVRSKAEDGIARLELHRPDCLNAMNRQLLRQLLAALEWAAANDAVRAVLITGHGR
CCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCE
VFSAGADIRYLNRAPAAEVRELARLAVAVTGRIEALGKPVLAALNGDALGGGLEIAEACT
EEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCHHHHHHHH
LRVAASHARFGHPEVKIGAVAGFGGTTRLPRLIGKGRAAEMLLTGRLIDADEACRLGLVN
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCHHHHHHCCCEECCHHHHHHHHHH
RVVPADDLIAESEALLSEVLAQSPLAVRLSWEAMHRGLSLSEDESARLGADYFGLAAQSE
HCCCCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCCCCCCCCHHHCCCCCCC
DFRIGTRAFLDKRAPKFKGR
CCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLERDVVRSKAEDGIARLELHRPDCLNAMNRQLLRQLLAALEWAAANDAVRAVLITGHGR
CCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCE
VFSAGADIRYLNRAPAAEVRELARLAVAVTGRIEALGKPVLAALNGDALGGGLEIAEACT
EEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCHHHHHHHH
LRVAASHARFGHPEVKIGAVAGFGGTTRLPRLIGKGRAAEMLLTGRLIDADEACRLGLVN
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCHHHHHHCCCEECCHHHHHHHHHH
RVVPADDLIAESEALLSEVLAQSPLAVRLSWEAMHRGLSLSEDESARLGADYFGLAAQSE
HCCCCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCCCCCCCCHHHCCCCCCC
DFRIGTRAFLDKRAPKFKGR
CCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA