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The map label for this gene is phoA2 [H]

Identifier: 34496968

GI number: 34496968

Start: 1615404

End: 1616777

Strand: Reverse

Name: phoA2 [H]

Synonym: CV_1513

Alternate gene names: 34496968

Gene position: 1616777-1615404 (Counterclockwise)

Preceding gene: 34496969

Following gene: 34496967

Centisome position: 34.03

GC content: 67.98

Gene sequence:

>1374_bases
ATGGAGAACGACAAGATGAAAACCGCGATGAACACCCTGCTGGCCGCCGCCGCGCTGGCCGCGCTGCCGGCCCTGCCCGC
CCACGCCGCCGGCGAGGCCAAGAACGTGATCTTTTTTCTCGGCGATGGCATGGGGCCCGCCACCGTCACCGCTGCCCGCA
TCTACCAGTACGGCGAGGGCGGCAAGCTCGCTATGGAGAAACTTGATCGCACCGCGCGCATCAAGACCTTCTCCAACGAC
GCGCAGACCACCGACAGCGCGCCGTCGATGTCGGCCTATATGACCGGCGTGAAGATGAACAACGAGGTGATCTCGATGTC
GTCCGACACCCGCGCCATCGAGCCCAACAGCGACGGCACCGGCAACTGCGGCAGCAATAACGGCGCGCCGGCCGCCACGC
TGCTGGAGCTGGCCAAGGCCAAAGGCAAGGCCATCGGCGCGGTCACCACCACCCGCGTCACCCACGCCACGCCGGCGGCC
ACCTACGCCCACGTCTGCCACCGCGACGCCGAATCCGACATCATCGCCCAGGCGGTGCCCGGCGGCGCCGGCTACAACGC
CAGGCTGGGCAACGGCCTCGACGTGCTGATGGGCGGCGGCAGCAAGTTCCTGCTGCCCAAAGCCAGCGGCGGCAAGCGCG
GCGACGGCCGCAACCTGCTGCAGGAATTCGCCGCCAAGGGCTATCCGGTGCTGCAGACCGGCAGCGATCTGGCCGCCTTC
GACGCCAAGTCGGCCAGCCGCGTGGTGGGCATCTTCGGCAAGGACCACCTGGAATACGAGCTGGACCGCGTCAAGAACAA
GGTGGACCAGCCCAGCCTGGCGCAGATGACCGCGGCGGCGATAGACGTGCTGTCCAAGAACGGCAACGGCTACGTGCTGA
TGGTGGAAGGCGGCCGCATCGACCACGCGCTGCACGGCACCAACGCCAAGCGCGCGCTGACGGACGCCATCGCCTTCGAC
GACGCGATCAAGGCCGCGCTGGCCAAGGTGGACTTGAACAACACGCTGGTGGTCGTCACCGCCGACCACGACCACACCAT
GACCATCAACGGCTATTCCAAGCGCGGCAACCCCATCCACGACATCGCGCGCAGCTATGTGGACGGCCAGCCGGCCAAGG
ACGCCGACGGCGCCGTCTACACCACGCTGGTGTTCGGCAACGGCCCCAACCGCAAGCCGACCCGGGTGTCGGTGGACTCG
GCCGCCGCCACCGCCGACGACTACCAGCAGGAAACCGGCATCCGCCTGTCCAGCGAGACCCACGGCGGCGGCGACGTGAT
GCTGATGTCCGGCGGCGCCGGCAGCAAGGGCTTCAAGGGCGTGATGGACAACACCAGGGTGTTCGGCCTGGTGAAGTCCG
CGCTCGGCCTGTAA

Upstream 100 bases:

>100_bases
ATGTCTACATCGGCGCCATCGGCCAGGGCGCCGACAGCATCCACGGCTTTCTGGAAAACATAGAAGTGTTCGGCCTGGTC
AAGAAGGCCGCCGGCCTGTG

Downstream 100 bases:

>100_bases
TCCCCTCCCGCGCGCGGCCGCCCGGCCGCGCGTTCCGCATTGGAAGAAATCGATGAAAGTCTTGTCTCTCGCCGCGCTGG
CGTTCGCCGCCCAGCAGGCG

Product: alkaline phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MENDKMKTAMNTLLAAAALAALPALPAHAAGEAKNVIFFLGDGMGPATVTAARIYQYGEGGKLAMEKLDRTARIKTFSND
AQTTDSAPSMSAYMTGVKMNNEVISMSSDTRAIEPNSDGTGNCGSNNGAPAATLLELAKAKGKAIGAVTTTRVTHATPAA
TYAHVCHRDAESDIIAQAVPGGAGYNARLGNGLDVLMGGGSKFLLPKASGGKRGDGRNLLQEFAAKGYPVLQTGSDLAAF
DAKSASRVVGIFGKDHLEYELDRVKNKVDQPSLAQMTAAAIDVLSKNGNGYVLMVEGGRIDHALHGTNAKRALTDAIAFD
DAIKAALAKVDLNNTLVVVTADHDHTMTINGYSKRGNPIHDIARSYVDGQPAKDADGAVYTTLVFGNGPNRKPTRVSVDS
AAATADDYQQETGIRLSSETHGGGDVMLMSGGAGSKGFKGVMDNTRVFGLVKSALGL

Sequences:

>Translated_457_residues
MENDKMKTAMNTLLAAAALAALPALPAHAAGEAKNVIFFLGDGMGPATVTAARIYQYGEGGKLAMEKLDRTARIKTFSND
AQTTDSAPSMSAYMTGVKMNNEVISMSSDTRAIEPNSDGTGNCGSNNGAPAATLLELAKAKGKAIGAVTTTRVTHATPAA
TYAHVCHRDAESDIIAQAVPGGAGYNARLGNGLDVLMGGGSKFLLPKASGGKRGDGRNLLQEFAAKGYPVLQTGSDLAAF
DAKSASRVVGIFGKDHLEYELDRVKNKVDQPSLAQMTAAAIDVLSKNGNGYVLMVEGGRIDHALHGTNAKRALTDAIAFD
DAIKAALAKVDLNNTLVVVTADHDHTMTINGYSKRGNPIHDIARSYVDGQPAKDADGAVYTTLVFGNGPNRKPTRVSVDS
AAATADDYQQETGIRLSSETHGGGDVMLMSGGAGSKGFKGVMDNTRVFGLVKSALGL
>Mature_457_residues
MENDKMKTAMNTLLAAAALAALPALPAHAAGEAKNVIFFLGDGMGPATVTAARIYQYGEGGKLAMEKLDRTARIKTFSND
AQTTDSAPSMSAYMTGVKMNNEVISMSSDTRAIEPNSDGTGNCGSNNGAPAATLLELAKAKGKAIGAVTTTRVTHATPAA
TYAHVCHRDAESDIIAQAVPGGAGYNARLGNGLDVLMGGGSKFLLPKASGGKRGDGRNLLQEFAAKGYPVLQTGSDLAAF
DAKSASRVVGIFGKDHLEYELDRVKNKVDQPSLAQMTAAAIDVLSKNGNGYVLMVEGGRIDHALHGTNAKRALTDAIAFD
DAIKAALAKVDLNNTLVVVTADHDHTMTINGYSKRGNPIHDIARSYVDGQPAKDADGAVYTTLVFGNGPNRKPTRVSVDS
AAATADDYQQETGIRLSSETHGGGDVMLMSGGAGSKGFKGVMDNTRVFGLVKSALGL

Specific function: Specifically cleaves both streptomycin-6-phosphate and, more slowly, streptomycin-3''-phosphate during the biosynthesis of streptomycin [H]

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266292, Length=443, Percent_Identity=37.4717832957111, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI116734717, Length=449, Percent_Identity=36.9710467706013, Blast_Score=255, Evalue=6e-68,
Organism=Homo sapiens, GI157266296, Length=463, Percent_Identity=37.5809935205184, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI94721246, Length=463, Percent_Identity=37.5809935205184, Blast_Score=253, Evalue=4e-67,
Organism=Homo sapiens, GI294660770, Length=444, Percent_Identity=36.2612612612613, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI294660772, Length=400, Percent_Identity=35, Blast_Score=215, Evalue=6e-56,
Organism=Escherichia coli, GI48994877, Length=463, Percent_Identity=29.1576673866091, Blast_Score=134, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320689, Length=361, Percent_Identity=33.2409972299169, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI21358067, Length=448, Percent_Identity=37.7232142857143, Blast_Score=253, Evalue=3e-67,
Organism=Drosophila melanogaster, GI21355981, Length=438, Percent_Identity=36.986301369863, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24585213, Length=460, Percent_Identity=36.304347826087, Blast_Score=238, Evalue=7e-63,
Organism=Drosophila melanogaster, GI21355151, Length=441, Percent_Identity=35.6009070294785, Blast_Score=235, Evalue=6e-62,
Organism=Drosophila melanogaster, GI85725200, Length=457, Percent_Identity=35.2297592997812, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI85815833, Length=457, Percent_Identity=35.2297592997812, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI21355149, Length=445, Percent_Identity=34.8314606741573, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI19921912, Length=471, Percent_Identity=34.1825902335456, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24651554, Length=469, Percent_Identity=33.0490405117271, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI18859923, Length=435, Percent_Identity=34.7126436781609, Blast_Score=202, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24657835, Length=483, Percent_Identity=33.5403726708075, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24651556, Length=456, Percent_Identity=32.6754385964912, Blast_Score=199, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24657842, Length=461, Percent_Identity=32.7548806941432, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24657827, Length=475, Percent_Identity=33.2631578947368, Blast_Score=179, Evalue=4e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.39 [H]

Molecular weight: Translated: 47420; Mature: 47420

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENDKMKTAMNTLLAAAALAALPALPAHAAGEAKNVIFFLGDGMGPATVTAARIYQYGEG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHEEECCC
GKLAMEKLDRTARIKTFSNDAQTTDSAPSMSAYMTGVKMNNEVISMSSDTRAIEPNSDGT
CHHHHHHHCHHHEEEEECCCCCCCCCCCCCHHHEEEEEECCEEEEECCCCEEECCCCCCC
GNCGSNNGAPAATLLELAKAKGKAIGAVTTTRVTHATPAATYAHVCHRDAESDIIAQAVP
CCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCHHHHHHHHHCCCCCCHHEEECC
GGAGYNARLGNGLDVLMGGGSKFLLPKASGGKRGDGRNLLQEFAAKGYPVLQTGSDLAAF
CCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEECCCCCEEC
DAKSASRVVGIFGKDHLEYELDRVKNKVDQPSLAQMTAAAIDVLSKNGNGYVLMVEGGRI
CCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCE
DHALHGTNAKRALTDAIAFDDAIKAALAKVDLNNTLVVVTADHDHTMTINGYSKRGNPIH
EEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCHHH
DIARSYVDGQPAKDADGAVYTTLVFGNGPNRKPTRVSVDSAAATADDYQQETGIRLSSET
HHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCEEECCCHHCCHHHHHHHCCCEEECCC
HGGGDVMLMSGGAGSKGFKGVMDNTRVFGLVKSALGL
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MENDKMKTAMNTLLAAAALAALPALPAHAAGEAKNVIFFLGDGMGPATVTAARIYQYGEG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHEEECCC
GKLAMEKLDRTARIKTFSNDAQTTDSAPSMSAYMTGVKMNNEVISMSSDTRAIEPNSDGT
CHHHHHHHCHHHEEEEECCCCCCCCCCCCCHHHEEEEEECCEEEEECCCCEEECCCCCCC
GNCGSNNGAPAATLLELAKAKGKAIGAVTTTRVTHATPAATYAHVCHRDAESDIIAQAVP
CCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCHHHHHHHHHCCCCCCHHEEECC
GGAGYNARLGNGLDVLMGGGSKFLLPKASGGKRGDGRNLLQEFAAKGYPVLQTGSDLAAF
CCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEECCCCCEEC
DAKSASRVVGIFGKDHLEYELDRVKNKVDQPSLAQMTAAAIDVLSKNGNGYVLMVEGGRI
CCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCE
DHALHGTNAKRALTDAIAFDDAIKAALAKVDLNNTLVVVTADHDHTMTINGYSKRGNPIH
EEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCHHH
DIARSYVDGQPAKDADGAVYTTLVFGNGPNRKPTRVSVDSAAATADDYQQETGIRLSSET
HHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCEEECCCHHCCHHHHHHHCCCEEECCC
HGGGDVMLMSGGAGSKGFKGVMDNTRVFGLVKSALGL
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1654502 [H]