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The map label for this gene is phoA1 [H]

Identifier: 34496969

GI number: 34496969

Start: 1616777

End: 1618312

Strand: Reverse

Name: phoA1 [H]

Synonym: CV_1514

Alternate gene names: 34496969

Gene position: 1618312-1616777 (Counterclockwise)

Preceding gene: 34496970

Following gene: 34496968

Centisome position: 34.06

GC content: 66.86

Gene sequence:

>1536_bases
ATGCCGCCTTATTTTGGCAGAAATGCGCGGAAAATATCATCTGTGCGCATTTTTAATAAAATAAGGTATGTCGTGATGCG
CACTTTCACTCTAGGCGCCGGCCTGCTGGCCGCGCTGACGCTCGCCGCGTGCAACAGCGACGGCGGCGGCAACGCCAATC
GTCCCCAGGACAATGCGGAAGCCCGCCCGCCGGTCGTCACCACCGCCAAGAACGTCATCTTCTTCCTCGGCGACGGCATG
GGCATCGCCACCACCACCGCCGCGCGCATCTACGCCGCCGGCGAGGATGGCGCGCTGACAATGGACACGCTGCCCGAGTC
CGGCTTCGTCAAAACCTTCTCCAACGACGCCCAGGTCACCGACAGCGCGCCGTCGATGTCGGCCTACATGACCGGCGTCA
AGATGAACAATGAAGTCATCTCCATGTCCACCGACACCGTGGCCAAAGCGCCGACCGCCGACCTGACCTCCGGCTGCGGC
GCCGGCAACGGCAAGCCGGTGTCCACGCTGCTGGAGCTGGCCAAGGCCGGCAACCGCGCCACCGGCGTGGTCACCACCAC
CCGCGTCACCCACGCCACCCCGGCCGCCACCTATGCCCACGTCTGCCACCGCGACGCCGAAGCCGACATCGCCGCCCAGC
TGGTGCCGGGCGGCGCGCTGTACAACGCCGCGCTGAAGGACGGCGTGGACGTGGTGTTCGGCGGCGGCCGCCAGTTCTTC
CTGCCCAAGACGGCAGGCGGCAAGCGCGCCGACGGCCGCGACCTGGTGGCGGAGCTGAAGGCCAAGGGCTATGGCTACGC
CGCCGACAAGGCCGCGTTCAGCGCCATCGACCCGGCCAAGACCCAGCGCGCGGTCGGCCTGTTCACCTCCAGCCACATGA
GCTACGACCTGGACCGCGACCCGAACAAGGAGCCCAGCCTGGCCGAGATGACCACCAAGGCCATCGACCTGCTGGCGAAT
CGCAGCGGCAAGCAAGGCATGTTCCTGATGGTGGAAGGCGGCCGCATCGACCACGCGCTGCACGAAACCACCGCCAAGAA
GGCGCTGCAGGACACCGTGGCCTTCGACCAGGCGATCAAGGCCGCCATCGACGCGATGCAGCAGAAGGACCCCGGCCTGA
AGAACACGCTGATCGTCGTCACCGCCGACCATGACCATACCCTGGTGCTCAACGGCTATGCCAAGCGCACCGGCAAGACC
GCGCCGGGCAATCCCGGCGTGCTGGGCCTGGCCAAGGACTACGTCAGCGGCAAGAACCTGACCGACGCCGACGGCATGCC
CTACTCCATCATCGGCTTCGGCAACGGCGAAAACCGCGTCGCCGGCAACCGCAACGCGGCGGCGGCGCTGACCGACGACG
CCGTCTCGGCCAACGACTACCACCAGGAAGCGGCGATACGGATGCCGACCGGCGGCGAAACCCACGGCGGCACCGATGTC
TACATCGGCGCCATCGGCCAGGGCGCCGACAGCATCCACGGCTTTCTGGAAAACATAGAAGTGTTCGGCCTGGTCAAGAA
GGCCGCCGGCCTGTGA

Upstream 100 bases:

>100_bases
AAGGTCGCCGCGCCCTGCCATGCCGGCGAGGCCAAGGTGCGGGTGTCCTTCCTCGCCCACCTGTGGAAGTGCTGAGCAAC
GCGTTCGTCATAAAATCTTA

Downstream 100 bases:

>100_bases
TGGAGAACGACAAGATGAAAACCGCGATGAACACCCTGCTGGCCGCCGCCGCGCTGGCCGCGCTGCCGGCCCTGCCCGCC
CACGCCGCCGGCGAGGCCAA

Product: alkaline phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MPPYFGRNARKISSVRIFNKIRYVVMRTFTLGAGLLAALTLAACNSDGGGNANRPQDNAEARPPVVTTAKNVIFFLGDGM
GIATTTAARIYAAGEDGALTMDTLPESGFVKTFSNDAQVTDSAPSMSAYMTGVKMNNEVISMSTDTVAKAPTADLTSGCG
AGNGKPVSTLLELAKAGNRATGVVTTTRVTHATPAATYAHVCHRDAEADIAAQLVPGGALYNAALKDGVDVVFGGGRQFF
LPKTAGGKRADGRDLVAELKAKGYGYAADKAAFSAIDPAKTQRAVGLFTSSHMSYDLDRDPNKEPSLAEMTTKAIDLLAN
RSGKQGMFLMVEGGRIDHALHETTAKKALQDTVAFDQAIKAAIDAMQQKDPGLKNTLIVVTADHDHTLVLNGYAKRTGKT
APGNPGVLGLAKDYVSGKNLTDADGMPYSIIGFGNGENRVAGNRNAAAALTDDAVSANDYHQEAAIRMPTGGETHGGTDV
YIGAIGQGADSIHGFLENIEVFGLVKKAAGL

Sequences:

>Translated_511_residues
MPPYFGRNARKISSVRIFNKIRYVVMRTFTLGAGLLAALTLAACNSDGGGNANRPQDNAEARPPVVTTAKNVIFFLGDGM
GIATTTAARIYAAGEDGALTMDTLPESGFVKTFSNDAQVTDSAPSMSAYMTGVKMNNEVISMSTDTVAKAPTADLTSGCG
AGNGKPVSTLLELAKAGNRATGVVTTTRVTHATPAATYAHVCHRDAEADIAAQLVPGGALYNAALKDGVDVVFGGGRQFF
LPKTAGGKRADGRDLVAELKAKGYGYAADKAAFSAIDPAKTQRAVGLFTSSHMSYDLDRDPNKEPSLAEMTTKAIDLLAN
RSGKQGMFLMVEGGRIDHALHETTAKKALQDTVAFDQAIKAAIDAMQQKDPGLKNTLIVVTADHDHTLVLNGYAKRTGKT
APGNPGVLGLAKDYVSGKNLTDADGMPYSIIGFGNGENRVAGNRNAAAALTDDAVSANDYHQEAAIRMPTGGETHGGTDV
YIGAIGQGADSIHGFLENIEVFGLVKKAAGL
>Mature_510_residues
PPYFGRNARKISSVRIFNKIRYVVMRTFTLGAGLLAALTLAACNSDGGGNANRPQDNAEARPPVVTTAKNVIFFLGDGMG
IATTTAARIYAAGEDGALTMDTLPESGFVKTFSNDAQVTDSAPSMSAYMTGVKMNNEVISMSTDTVAKAPTADLTSGCGA
GNGKPVSTLLELAKAGNRATGVVTTTRVTHATPAATYAHVCHRDAEADIAAQLVPGGALYNAALKDGVDVVFGGGRQFFL
PKTAGGKRADGRDLVAELKAKGYGYAADKAAFSAIDPAKTQRAVGLFTSSHMSYDLDRDPNKEPSLAEMTTKAIDLLANR
SGKQGMFLMVEGGRIDHALHETTAKKALQDTVAFDQAIKAAIDAMQQKDPGLKNTLIVVTADHDHTLVLNGYAKRTGKTA
PGNPGVLGLAKDYVSGKNLTDADGMPYSIIGFGNGENRVAGNRNAAAALTDDAVSANDYHQEAAIRMPTGGETHGGTDVY
IGAIGQGADSIHGFLENIEVFGLVKKAAGL

Specific function: Specifically cleaves both streptomycin-6-phosphate and, more slowly, streptomycin-3''-phosphate during the biosynthesis of streptomycin [H]

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=480, Percent_Identity=37.0833333333333, Blast_Score=252, Evalue=7e-67,
Organism=Homo sapiens, GI294660770, Length=475, Percent_Identity=36.4210526315789, Blast_Score=241, Evalue=8e-64,
Organism=Homo sapiens, GI157266296, Length=474, Percent_Identity=35.8649789029536, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI157266292, Length=490, Percent_Identity=34.6938775510204, Blast_Score=235, Evalue=8e-62,
Organism=Homo sapiens, GI94721246, Length=474, Percent_Identity=35.6540084388186, Blast_Score=234, Evalue=2e-61,
Organism=Homo sapiens, GI294660772, Length=443, Percent_Identity=34.7629796839729, Blast_Score=209, Evalue=4e-54,
Organism=Escherichia coli, GI48994877, Length=356, Percent_Identity=32.8651685393258, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320689, Length=339, Percent_Identity=32.7433628318584, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI85725200, Length=451, Percent_Identity=35.0332594235033, Blast_Score=237, Evalue=2e-62,
Organism=Drosophila melanogaster, GI85815833, Length=451, Percent_Identity=35.0332594235033, Blast_Score=237, Evalue=2e-62,
Organism=Drosophila melanogaster, GI21355151, Length=456, Percent_Identity=37.0614035087719, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI21358067, Length=499, Percent_Identity=35.2705410821643, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI19921912, Length=488, Percent_Identity=34.2213114754098, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI21355981, Length=467, Percent_Identity=33.6188436830835, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24585213, Length=466, Percent_Identity=33.4763948497854, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI21355149, Length=446, Percent_Identity=33.4080717488789, Blast_Score=209, Evalue=5e-54,
Organism=Drosophila melanogaster, GI18859923, Length=452, Percent_Identity=35.3982300884956, Blast_Score=208, Evalue=6e-54,
Organism=Drosophila melanogaster, GI24651554, Length=485, Percent_Identity=34.2268041237113, Blast_Score=208, Evalue=9e-54,
Organism=Drosophila melanogaster, GI24657827, Length=478, Percent_Identity=33.6820083682008, Blast_Score=199, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24651556, Length=473, Percent_Identity=33.615221987315, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24657835, Length=494, Percent_Identity=31.7813765182186, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24657842, Length=495, Percent_Identity=30.1010101010101, Blast_Score=179, Evalue=4e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.39 [H]

Molecular weight: Translated: 53208; Mature: 53076

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPYFGRNARKISSVRIFNKIRYVVMRTFTLGAGLLAALTLAACNSDGGGNANRPQDNAE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
ARPPVVTTAKNVIFFLGDGMGIATTTAARIYAAGEDGALTMDTLPESGFVKTFSNDAQVT
CCCCEEEECCEEEEEEECCCCEEEECEEEEEEECCCCEEECCCCCCCCCEEEECCCCEEC
DSAPSMSAYMTGVKMNNEVISMSTDTVAKAPTADLTSGCGAGNGKPVSTLLELAKAGNRA
CCCCCCCHHEEEEEECCEEEEECCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCC
TGVVTTTRVTHATPAATYAHVCHRDAEADIAAQLVPGGALYNAALKDGVDVVFGGGRQFF
CEEEEEEEEECCCCHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCEEEECCCEEEE
LPKTAGGKRADGRDLVAELKAKGYGYAADKAAFSAIDPAKTQRAVGLFTSSHMSYDLDRD
EECCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHEEEECCCCEEECCCC
PNKEPSLAEMTTKAIDLLANRSGKQGMFLMVEGGRIDHALHETTAKKALQDTVAFDQAIK
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAIDAMQQKDPGLKNTLIVVTADHDHTLVLNGYAKRTGKTAPGNPGVLGLAKDYVSGKNL
HHHHHHHHCCCCCCCEEEEEECCCCCEEEEECHHHHCCCCCCCCCCEEEEHHHHCCCCCC
TDADGMPYSIIGFGNGENRVAGNRNAAAALTDDAVSANDYHQEAAIRMPTGGETHGGTDV
CCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCHHCCCEEECCCCCCCCCCCEE
YIGAIGQGADSIHGFLENIEVFGLVKKAAGL
EEEECCCCHHHHHHHHHHHHHEEHHHHHCCC
>Mature Secondary Structure 
PPYFGRNARKISSVRIFNKIRYVVMRTFTLGAGLLAALTLAACNSDGGGNANRPQDNAE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
ARPPVVTTAKNVIFFLGDGMGIATTTAARIYAAGEDGALTMDTLPESGFVKTFSNDAQVT
CCCCEEEECCEEEEEEECCCCEEEECEEEEEEECCCCEEECCCCCCCCCEEEECCCCEEC
DSAPSMSAYMTGVKMNNEVISMSTDTVAKAPTADLTSGCGAGNGKPVSTLLELAKAGNRA
CCCCCCCHHEEEEEECCEEEEECCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCC
TGVVTTTRVTHATPAATYAHVCHRDAEADIAAQLVPGGALYNAALKDGVDVVFGGGRQFF
CEEEEEEEEECCCCHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCEEEECCCEEEE
LPKTAGGKRADGRDLVAELKAKGYGYAADKAAFSAIDPAKTQRAVGLFTSSHMSYDLDRD
EECCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHEEEECCCCEEECCCC
PNKEPSLAEMTTKAIDLLANRSGKQGMFLMVEGGRIDHALHETTAKKALQDTVAFDQAIK
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAIDAMQQKDPGLKNTLIVVTADHDHTLVLNGYAKRTGKTAPGNPGVLGLAKDYVSGKNL
HHHHHHHHCCCCCCCEEEEEECCCCCEEEEECHHHHCCCCCCCCCCEEEEHHHHCCCCCC
TDADGMPYSIIGFGNGENRVAGNRNAAAALTDDAVSANDYHQEAAIRMPTGGETHGGTDV
CCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCHHCCCEEECCCCCCCCCCCEE
YIGAIGQGADSIHGFLENIEVFGLVKKAAGL
EEEECCCCHHHHHHHHHHHHHEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1654502 [H]