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The map label for this gene is entE [H]

Identifier: 34496939

GI number: 34496939

Start: 1581380

End: 1582987

Strand: Reverse

Name: entE [H]

Synonym: CV_1484

Alternate gene names: 34496939

Gene position: 1582987-1581380 (Counterclockwise)

Preceding gene: 34496940

Following gene: 34496938

Centisome position: 33.32

GC content: 70.34

Gene sequence:

>1608_bases
ATGAGCGTCGCCTTCACCCGCTGGCCGGATGACCTGGCCGCGCGCTACCGCGCCGCCGGCTACTGGAACGACCAGCCGAT
GACCGAGATGCTGGCGCGGCAATGCCGCGAGCGTCCCGACGCGCCCGCCGTGCTGTGCGGCGAGCGCGAGATCAGCTACG
CCGAGCTGGACCGCCGCGCGGGCAACCTGGCCGCCTGGCTGCTGGCGCGCGGCGTGGCCCGCCACGACACCGCGCTGGTG
CAGCTGCCGAATGTCGCCGAGTTCTACATCGCGTTGTTCGCGCTGTTCAAGATCGGCGCCGCGCCGGTCAACGCGCTGTT
CAGCCACCAGAAGCTGGAGCTGTCGGCCTATGCCCGCCAGATCCAGCCCAAGCTGCTGATCGCCGACCGCCGCCATTCGC
TGTTCGGCGACGACGCGATGGCGGACGAGCTGGCCGCGATCTCGCCGGCGCTGAGCGTGCGCCTGTTCGCCCACGACGAG
CTGGAAACCGTACTGGAGCGCGACTGCGGCGGCCTGCCGGACCAAGCCGGCCCCAGCGCCGCCGACGAAGTGGCGTTCTT
CCAGCTGTCCGGCGGCAGCACCGGCACGCCCAAGCTGATTCCGCGCACCCACAACGACTACTTCTACAGCGTGCGCCGCA
GCGCCGAGATCTGCGAACTCGGCCCGCATACCCGCTTCCTGTGCGCGCTGCCTGCGCCGCACAACTTCCCGCTCAGCTCG
CCGGGCGCGCTCGGCGTGTTCCACGCCGGCGGCGCGGTGGTGCTGGCGCCCAATCCGGAAGCGCTGTCCTGCTTCGCGCT
GGTGGAGCGCCACCGGGTGGACATCGCCGCGCTGGTGCCGCCGGCCGTCGCGCTGTGGCTGCAGGCCGCGCCCGGCCGCG
AGGCCCAGCTGAAAAGCCTGAAACTGCTGCAGGTCGGCGGCGCCAGCTTCGCCGAAGCCACCGCCCGCCAGGTGCCGGAA
GTGCTGGGCTGCAAACTGCAGCAGGTGTTCGGCATGGCCGAGGGCCTGGTCAACTACACCCGCCTGGACGACGGCGATGA
CATCGTCTATGGCTGCCAGGGCCGGCCGATGAGCGATGCCGACGAGGTGAAGGTGGTGGACGAAGCCGGCCATCCGGTGC
CGACCGGCACGCCGGGCATGCTGGCCACCCGCGGTCCCTACACCTTCCGCGGCTACTACCTGGCGCCGGAGCACAACGCC
CGCGTGTTCGACAACGAGGGATTCTATTACTCGGGCGACGTGGTGGTGGCCGACGAGCGCGGCTACCTGCGCGTGGTCGG
CCGGGTGAAGGACCAGATCAATCGCGGCGGCGAGAAGATCGCCGCCGAGGAAATCGAACACCTGCTGCTGCAACACCCGC
ACGTGGCCCAGGCCGCGCTGGTGGCGATGCCCGACGCGATGCTGGGCGAGAAGAGCTGCGCCTTCATCGTGCCGCGTGGC
GACGGTCTGAAGTCGGTGGCGCTGCGCCGCTTCCTGCGCGAGCAGGGCGTGGCCGACTACAAGCTGCCCGACCGTTTCGA
ACTGCTAGACACCCTGCCACTGACCCACGTCGGCAAAATAGACAAACAAGCGCTGCGCGGCATGCTGGCCGCCGCGGCCG
GAGCCTGA

Upstream 100 bases:

>100_bases
TCGTCGCCGCTTCCACGCCGGAAGCGGAATGGGCGGAAACCCAGGCCAAGCTCGGCACCATGCTGGGTGCCTTCGGCCTG
TCCGGCCAGGAGGCGGTGGC

Downstream 100 bases:

>100_bases
ACACCATGAGCATTCCCAAGCTGAACGCCTACCCGCTGCCGCAGGCGGCCGACTTCCCCGCCAACAAGACCGCCTGGCGC
GTGGATCCCAAGCGCGCCGC

Product: enterobactin synthase subunit E

Products: NA

Alternate protein names: Enterochelin synthase E; 2,3-dihydroxybenzoate-AMP ligase; Dihydroxybenzoic acid-activating enzyme; S-dihydroxybenzoyltransferase [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MSVAFTRWPDDLAARYRAAGYWNDQPMTEMLARQCRERPDAPAVLCGEREISYAELDRRAGNLAAWLLARGVARHDTALV
QLPNVAEFYIALFALFKIGAAPVNALFSHQKLELSAYARQIQPKLLIADRRHSLFGDDAMADELAAISPALSVRLFAHDE
LETVLERDCGGLPDQAGPSAADEVAFFQLSGGSTGTPKLIPRTHNDYFYSVRRSAEICELGPHTRFLCALPAPHNFPLSS
PGALGVFHAGGAVVLAPNPEALSCFALVERHRVDIAALVPPAVALWLQAAPGREAQLKSLKLLQVGGASFAEATARQVPE
VLGCKLQQVFGMAEGLVNYTRLDDGDDIVYGCQGRPMSDADEVKVVDEAGHPVPTGTPGMLATRGPYTFRGYYLAPEHNA
RVFDNEGFYYSGDVVVADERGYLRVVGRVKDQINRGGEKIAAEEIEHLLLQHPHVAQAALVAMPDAMLGEKSCAFIVPRG
DGLKSVALRRFLREQGVADYKLPDRFELLDTLPLTHVGKIDKQALRGMLAAAAGA

Sequences:

>Translated_535_residues
MSVAFTRWPDDLAARYRAAGYWNDQPMTEMLARQCRERPDAPAVLCGEREISYAELDRRAGNLAAWLLARGVARHDTALV
QLPNVAEFYIALFALFKIGAAPVNALFSHQKLELSAYARQIQPKLLIADRRHSLFGDDAMADELAAISPALSVRLFAHDE
LETVLERDCGGLPDQAGPSAADEVAFFQLSGGSTGTPKLIPRTHNDYFYSVRRSAEICELGPHTRFLCALPAPHNFPLSS
PGALGVFHAGGAVVLAPNPEALSCFALVERHRVDIAALVPPAVALWLQAAPGREAQLKSLKLLQVGGASFAEATARQVPE
VLGCKLQQVFGMAEGLVNYTRLDDGDDIVYGCQGRPMSDADEVKVVDEAGHPVPTGTPGMLATRGPYTFRGYYLAPEHNA
RVFDNEGFYYSGDVVVADERGYLRVVGRVKDQINRGGEKIAAEEIEHLLLQHPHVAQAALVAMPDAMLGEKSCAFIVPRG
DGLKSVALRRFLREQGVADYKLPDRFELLDTLPLTHVGKIDKQALRGMLAAAAGA
>Mature_534_residues
SVAFTRWPDDLAARYRAAGYWNDQPMTEMLARQCRERPDAPAVLCGEREISYAELDRRAGNLAAWLLARGVARHDTALVQ
LPNVAEFYIALFALFKIGAAPVNALFSHQKLELSAYARQIQPKLLIADRRHSLFGDDAMADELAAISPALSVRLFAHDEL
ETVLERDCGGLPDQAGPSAADEVAFFQLSGGSTGTPKLIPRTHNDYFYSVRRSAEICELGPHTRFLCALPAPHNFPLSSP
GALGVFHAGGAVVLAPNPEALSCFALVERHRVDIAALVPPAVALWLQAAPGREAQLKSLKLLQVGGASFAEATARQVPEV
LGCKLQQVFGMAEGLVNYTRLDDGDDIVYGCQGRPMSDADEVKVVDEAGHPVPTGTPGMLATRGPYTFRGYYLAPEHNAR
VFDNEGFYYSGDVVVADERGYLRVVGRVKDQINRGGEKIAAEEIEHLLLQHPHVAQAALVAMPDAMLGEKSCAFIVPRGD
GLKSVALRRFLREQGVADYKLPDRFELLDTLPLTHVGKIDKQALRGMLAAAAGA

Specific function: Activates the carboxylate group of 2,3-dihydroxy- benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-EntB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. EntE subfamily [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=528, Percent_Identity=25.9469696969697, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI156151445, Length=546, Percent_Identity=24.3589743589744, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI157311624, Length=507, Percent_Identity=24.0631163708087, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI157311622, Length=507, Percent_Identity=24.0631163708087, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI58082049, Length=516, Percent_Identity=24.2248062015504, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI122937307, Length=259, Percent_Identity=31.2741312741313, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI28416953, Length=547, Percent_Identity=24.1316270566728, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI42544132, Length=533, Percent_Identity=24.390243902439, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI115511026, Length=510, Percent_Identity=25.8823529411765, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI187761345, Length=376, Percent_Identity=25.7978723404255, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI187761343, Length=376, Percent_Identity=25.7978723404255, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI8923896, Length=543, Percent_Identity=22.0994475138122, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1786810, Length=540, Percent_Identity=58.5185185185185, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI145693145, Length=539, Percent_Identity=32.2820037105751, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1788107, Length=566, Percent_Identity=26.3250883392226, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI221142682, Length=486, Percent_Identity=26.5432098765432, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1790505, Length=554, Percent_Identity=24.187725631769, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786529, Length=556, Percent_Identity=23.021582733813, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786801, Length=453, Percent_Identity=25.8278145695364, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1789201, Length=364, Percent_Identity=24.4505494505494, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538037, Length=523, Percent_Identity=27.5334608030593, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17557194, Length=554, Percent_Identity=25.812274368231, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17560308, Length=550, Percent_Identity=24.9090909090909, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71994703, Length=529, Percent_Identity=24.1965973534972, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71994694, Length=529, Percent_Identity=24.1965973534972, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71994690, Length=529, Percent_Identity=24.1965973534972, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=541, Percent_Identity=22.735674676525, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=504, Percent_Identity=23.6111111111111, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17558820, Length=496, Percent_Identity=24.5967741935484, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=505, Percent_Identity=24.5544554455446, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17560140, Length=367, Percent_Identity=24.5231607629428, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71983001, Length=545, Percent_Identity=24.7706422018349, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71982997, Length=545, Percent_Identity=24.7706422018349, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71985884, Length=348, Percent_Identity=24.4252873563218, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=533, Percent_Identity=23.2645403377111, Blast_Score=119, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6319264, Length=574, Percent_Identity=24.2160278745645, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323182, Length=574, Percent_Identity=22.6480836236934, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320852, Length=496, Percent_Identity=18.75, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24581924, Length=519, Percent_Identity=25.4335260115607, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI19922652, Length=534, Percent_Identity=23.4082397003745, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21355181, Length=496, Percent_Identity=23.7903225806452, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI18859661, Length=539, Percent_Identity=25.417439703154, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355225, Length=552, Percent_Identity=21.3768115942029, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648260, Length=269, Percent_Identity=26.7657992565056, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24656500, Length=511, Percent_Identity=23.8747553816047, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356947, Length=504, Percent_Identity=21.6269841269841, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI62472339, Length=538, Percent_Identity=22.3048327137546, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24667955, Length=538, Percent_Identity=22.3048327137546, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21358303, Length=483, Percent_Identity=21.5320910973085, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24667959, Length=370, Percent_Identity=24.3243243243243, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281365686, Length=271, Percent_Identity=25.4612546125461, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648257, Length=379, Percent_Identity=25.8575197889182, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24653035, Length=271, Percent_Identity=24.3542435424354, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648253, Length=519, Percent_Identity=19.8458574181118, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648255, Length=518, Percent_Identity=19.8841698841699, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24666501, Length=202, Percent_Identity=32.1782178217822, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24666497, Length=202, Percent_Identity=32.1782178217822, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281366413, Length=202, Percent_Identity=32.1782178217822, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161076582, Length=282, Percent_Identity=24.113475177305, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =2.7.7.58 [H]

Molecular weight: Translated: 58081; Mature: 57950

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAFTRWPDDLAARYRAAGYWNDQPMTEMLARQCRERPDAPAVLCGEREISYAELDRRA
CCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHC
GNLAAWLLARGVARHDTALVQLPNVAEFYIALFALFKIGAAPVNALFSHQKLELSAYARQ
CHHHHHHHHHCHHHCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHH
IQPKLLIADRRHSLFGDDAMADELAAISPALSVRLFAHDELETVLERDCGGLPDQAGPSA
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCC
ADEVAFFQLSGGSTGTPKLIPRTHNDYFYSVRRSAEICELGPHTRFLCALPAPHNFPLSS
CCCEEEEEECCCCCCCCCCCCCCCCCHHEEHHCCCHHHHCCCCCEEEEECCCCCCCCCCC
PGALGVFHAGGAVVLAPNPEALSCFALVERHRVDIAALVPPAVALWLQAAPGREAQLKSL
CCCEEEEECCCEEEECCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCHHHHHHE
KLLQVGGASFAEATARQVPEVLGCKLQQVFGMAEGLVNYTRLDDGDDIVYGCQGRPMSDA
EEEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCH
DEVKVVDEAGHPVPTGTPGMLATRGPYTFRGYYLAPEHNARVFDNEGFYYSGDVVVADER
HHEEEECCCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCEEEECCCCEEECCEEEECCC
GYLRVVGRVKDQINRGGEKIAAEEIEHLLLQHPHVAQAALVAMPDAMLGEKSCAFIVPRG
CCEEHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHCCCCCEEEEEECC
DGLKSVALRRFLREQGVADYKLPDRFELLDTLPLTHVGKIDKQALRGMLAAAAGA
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVAFTRWPDDLAARYRAAGYWNDQPMTEMLARQCRERPDAPAVLCGEREISYAELDRRA
CCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHC
GNLAAWLLARGVARHDTALVQLPNVAEFYIALFALFKIGAAPVNALFSHQKLELSAYARQ
CHHHHHHHHHCHHHCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHH
IQPKLLIADRRHSLFGDDAMADELAAISPALSVRLFAHDELETVLERDCGGLPDQAGPSA
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCC
ADEVAFFQLSGGSTGTPKLIPRTHNDYFYSVRRSAEICELGPHTRFLCALPAPHNFPLSS
CCCEEEEEECCCCCCCCCCCCCCCCCHHEEHHCCCHHHHCCCCCEEEEECCCCCCCCCCC
PGALGVFHAGGAVVLAPNPEALSCFALVERHRVDIAALVPPAVALWLQAAPGREAQLKSL
CCCEEEEECCCEEEECCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCHHHHHHE
KLLQVGGASFAEATARQVPEVLGCKLQQVFGMAEGLVNYTRLDDGDDIVYGCQGRPMSDA
EEEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCH
DEVKVVDEAGHPVPTGTPGMLATRGPYTFRGYYLAPEHNARVFDNEGFYYSGDVVVADER
HHEEEECCCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCEEEECCCCEEECCEEEECCC
GYLRVVGRVKDQINRGGEKIAAEEIEHLLLQHPHVAQAALVAMPDAMLGEKSCAFIVPRG
CCEEHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHCCCCCEEEEEECC
DGLKSVALRRFLREQGVADYKLPDRFELLDTLPLTHVGKIDKQALRGMLAAAAGA
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]