Click here to switch to the map view.

The map label for this gene is entB [H]

Identifier: 34496938

GI number: 34496938

Start: 1580496

End: 1581374

Strand: Reverse

Name: entB [H]

Synonym: CV_1483

Alternate gene names: 34496938

Gene position: 1581374-1580496 (Counterclockwise)

Preceding gene: 34496939

Following gene: 34496937

Centisome position: 33.28

GC content: 67.69

Gene sequence:

>879_bases
ATGAGCATTCCCAAGCTGAACGCCTACCCGCTGCCGCAGGCGGCCGACTTCCCCGCCAACAAGACCGCCTGGCGCGTGGA
TCCCAAGCGCGCCGCGCTGCTGATCCACGACATGCAGCGCTACTTCCTCGCCTTCTACGGCGAGGACAGCGCGCTGGTGA
ACGAACTGGTTTCCAAGGTGGAGGCGCTGCGCCGCTGGGCCGACAGCCACGGCGTGCCGGTGATCTACACCGCGCAGCCG
ACCGAGCAGAAGCCGGAAGACCGCGCGCTGCTGAACGATATGTGGGGCCCCGGCCTCACCACCGCCGATCCGGCGGTGCA
GGCGGTGACGCCGCGGCTGGCCCCGCGCGAGCGGGACACCGTGCTGGTCAAATGGCGCTACAGCGCCTTCCAGCGCAGCG
ATCTGCAGGAAAAAATGAAAGCCTGGGGCCGCGACCAGCTGGTGATCTGCGGCGTCTACGCCCACATCGGCTGCATGATG
ACCGCCTGCGACGCCTTCATGCGCGACATCCAGGCCTTCATGGTCGGCGACGCGGTGGCGGATTTCAGCGAGGAAGAGCA
CAAGATGGCGTTGCGCTACGTGGCCACCCGCTGCGGCGCGGTGATCGCGCAATCCGACCTGGCGGCGGCCGGCGGCGACG
CCGCGCTGACCCGCGAATGGCTGAAGGCCCAGGTGCTGACGGTGCTGGAAGACGGCGACGACAGCCTGGCCGGCGATGAC
AACCTGCTGGACTACGGCCTGGACTCCATCCGCGTGATGGAGCTGGTGGCGCAATGGCAGAAGCTGGGCCTGGAAATCGG
CTTCGAAGATTTGGCCCAAGACCTGACGCTGGACGGCTGGTGGACCGCCATCCAGGCCAAGCTGCCGCAGGAGGCCTGA

Upstream 100 bases:

>100_bases
GTTTCGAACTGCTAGACACCCTGCCACTGACCCACGTCGGCAAAATAGACAAACAAGCGCTGCGCGGCATGCTGGCCGCC
GCGGCCGGAGCCTGAACACC

Downstream 100 bases:

>100_bases
TCATGGTCGGACTGGATTTCACCGGCCAATGCGTGTGGGTGACCGGCGCGGCCCAGGGCATAGGCCAGGAAGTGGCGCGC
CGCTTCGTCGAGGCCGGCGC

Product: isochorismatase

Products: NA

Alternate protein names: 2,3 dihydro-2,3 dihydroxybenzoate synthase; Enterobactin synthase component B; Enterochelin synthase B; Isochorismate lyase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSIPKLNAYPLPQAADFPANKTAWRVDPKRAALLIHDMQRYFLAFYGEDSALVNELVSKVEALRRWADSHGVPVIYTAQP
TEQKPEDRALLNDMWGPGLTTADPAVQAVTPRLAPRERDTVLVKWRYSAFQRSDLQEKMKAWGRDQLVICGVYAHIGCMM
TACDAFMRDIQAFMVGDAVADFSEEEHKMALRYVATRCGAVIAQSDLAAAGGDAALTREWLKAQVLTVLEDGDDSLAGDD
NLLDYGLDSIRVMELVAQWQKLGLEIGFEDLAQDLTLDGWWTAIQAKLPQEA

Sequences:

>Translated_292_residues
MSIPKLNAYPLPQAADFPANKTAWRVDPKRAALLIHDMQRYFLAFYGEDSALVNELVSKVEALRRWADSHGVPVIYTAQP
TEQKPEDRALLNDMWGPGLTTADPAVQAVTPRLAPRERDTVLVKWRYSAFQRSDLQEKMKAWGRDQLVICGVYAHIGCMM
TACDAFMRDIQAFMVGDAVADFSEEEHKMALRYVATRCGAVIAQSDLAAAGGDAALTREWLKAQVLTVLEDGDDSLAGDD
NLLDYGLDSIRVMELVAQWQKLGLEIGFEDLAQDLTLDGWWTAIQAKLPQEA
>Mature_291_residues
SIPKLNAYPLPQAADFPANKTAWRVDPKRAALLIHDMQRYFLAFYGEDSALVNELVSKVEALRRWADSHGVPVIYTAQPT
EQKPEDRALLNDMWGPGLTTADPAVQAVTPRLAPRERDTVLVKWRYSAFQRSDLQEKMKAWGRDQLVICGVYAHIGCMMT
ACDAFMRDIQAFMVGDAVADFSEEEHKMALRYVATRCGAVIAQSDLAAAGGDAALTREWLKAQVLTVLEDGDDSLAGDDN
LLDYGLDSIRVMELVAQWQKLGLEIGFEDLAQDLTLDGWWTAIQAKLPQEA

Specific function: Required for production of 2,3-DHB. Also serves as an aryl carrier protein and plays a role in enterobactin assembly [H]

COG id: COG1535

COG function: function code Q; Isochorismate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Escherichia coli, GI1786811, Length=288, Percent_Identity=53.125, Blast_Score=313, Evalue=9e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009081
- InterPro:   IPR016291
- InterPro:   IPR000868
- InterPro:   IPR006163 [H]

Pfam domain/function: PF00857 Isochorismatase; PF00550 PP-binding [H]

EC number: =3.3.2.1 [H]

Molecular weight: Translated: 32504; Mature: 32373

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPKLNAYPLPQAADFPANKTAWRVDPKRAALLIHDMQRYFLAFYGEDSALVNELVSKV
CCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EALRRWADSHGVPVIYTAQPTEQKPEDRALLNDMWGPGLTTADPAVQAVTPRLAPRERDT
HHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCE
VLVKWRYSAFQRSDLQEKMKAWGRDQLVICGVYAHIGCMMTACDAFMRDIQAFMVGDAVA
EEEEEEHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFSEEEHKMALRYVATRCGAVIAQSDLAAAGGDAALTREWLKAQVLTVLEDGDDSLAGDD
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
NLLDYGLDSIRVMELVAQWQKLGLEIGFEDLAQDLTLDGWWTAIQAKLPQEA
CHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
SIPKLNAYPLPQAADFPANKTAWRVDPKRAALLIHDMQRYFLAFYGEDSALVNELVSKV
CCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EALRRWADSHGVPVIYTAQPTEQKPEDRALLNDMWGPGLTTADPAVQAVTPRLAPRERDT
HHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCE
VLVKWRYSAFQRSDLQEKMKAWGRDQLVICGVYAHIGCMMTACDAFMRDIQAFMVGDAVA
EEEEEEHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFSEEEHKMALRYVATRCGAVIAQSDLAAAGGDAALTREWLKAQVLTVLEDGDDSLAGDD
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
NLLDYGLDSIRVMELVAQWQKLGLEIGFEDLAQDLTLDGWWTAIQAKLPQEA
CHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]