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The map label for this gene is nusA [H]

Identifier: 34496916

GI number: 34496916

Start: 1549476

End: 1550954

Strand: Direct

Name: nusA [H]

Synonym: CV_1461

Alternate gene names: 34496916

Gene position: 1549476-1550954 (Clockwise)

Preceding gene: 34496915

Following gene: 34496917

Centisome position: 32.61

GC content: 64.5

Gene sequence:

>1479_bases
ATGAGTCGCGAGATTTTGTTGCTGGTGGATGCCCTGGCGAGCGAGAAGAATGTCAGCAAGGATGTGGTGTTTTCCGCCCT
TGAGATGGCGCTTGCCTCGGCCACCAAGAAGAAGTTTACCGATGACGAGATGGATGTGCGCGTCGAGATCGACCGCCACA
CCGGTCAGTACCAGACCTTCCGCCGCTGGCTGGTGGTGGAGGACGAGCTGCTGGAATCCGAAAGCAAGGAAATCGGCCTG
ATCGACGCGCGCGACCGCGATCCGCGCATCGAGTTGGGCGCCTTCATCGAAGAGCCGATGGAGGCCGTCGAATTCGGCCG
CATCGGCGCGCAGACCGCCAAGCAGGTGATCCTGCAGCGCATCCGCGACGCCGAGCGCGAACAGCTGCTGAACGAATTCC
TGCAGCGCCGCGAGCACCTGGTGATCGGCACCATCAAGCGCATCGAGCGCGGCAACGCCATCGTCGAGTGCGGCAAGCTG
GAAGCCGTGCTGCCGCGCGACCAGATGATTCCGAAGGAAAACCTGCGCGTGGGCGACCGCGTGAAGGCCTATCTGCTGCG
CATCGACCGCCAGGGCCGCGGCCCGCAACTGATCCTGTCGCGCACCGCGCCGGAATTCCTGGTCAAGCTGTTCGAGCTGG
AAGTGCCGGAGATCGAGGAAGGCATGCTGGAAATCCGCGCCGCCGTGCGCGATCCGGGCATGCGCGCCAAGATCGCCGTC
AAGGCCAACGACGCCCGCATCGATCCGCAGGGCACTTGCATCGGCATGCGCGGCTCGCGCGTGCAGGCGGTGACCCAGGA
ACTGGCGGGCGAGCGCGTCGACATCGTGCTGTGGGCGCCGGAACCGGCCCAGTTCGTGATCAACGCGCTGTCGCCGGCCG
AAGTCAGCCGCATCATGGTGGACGAAGACAATCACAGCATGGACGTGATCGTCGAGGAAGAGCAGCTGGCGCTGGCCATC
GGCCGCAGCGGGCAGAACGTCAAGCTCGCCGCCGAGCTGACCGGCTGGTACCTGAACATCATGACCGTGGCCGAAGCGGA
AGAGAAGCATCAGGCCGAAGACGCCGCGCTGCGCCAGCTGTTCGTCAGCGCGCTGAGCGTGGATGAAGACACCGCCGCCA
AACTGGTGGAAGAGGGCTTCGCCGCGCTGGAGGAAGTGGCCTACGTGCCGCTGGAAGAAATGCTGGAAATCGAAGGCTTC
AACGAGGAGCTGGTGGGCGAGCTTCGCAGCCGCGCCCGTGATGCGCTCCTGACGCAGGCCATCGCGTCCGAAGAGCAGGT
CGAGAACGTAGCCGACGACCTGAAGGACGTTGAAGGCCTGGATGCCGAGCTGGTTCGCAAGCTGGCCGCAGGCGGCGTGA
CCACCCGCGACGATCTGGCCGACCTGGCCGTTGACGATCTGGTGGACATGACCGGCATGGAAGCGGAAGCTGCACGCGCA
ATCATCATGAAGGCGCGCGAACACTGGTTCAACCAGTAA

Upstream 100 bases:

>100_bases
ATTCCGTTCGCCAACATCGACAAGGCTCGTCTCGAGCCTGAGTTTTGATGTCTGGACGGCTTTTAGCCCACGCTAAAGGA
CAAAATTCGGAGAGGAATGC

Downstream 100 bases:

>100_bases
GGCTTTCTCGGGAAGTATTTACGGAATTTGGGAAGACGCATGGTTTTGACGAATGTAAAACAATTTGCCAGCGAGATGAA
TCTCACGCCGGAACGCCTGC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSREILLLVDALASEKNVSKDVVFSALEMALASATKKKFTDDEMDVRVEIDRHTGQYQTFRRWLVVEDELLESESKEIGL
IDARDRDPRIELGAFIEEPMEAVEFGRIGAQTAKQVILQRIRDAEREQLLNEFLQRREHLVIGTIKRIERGNAIVECGKL
EAVLPRDQMIPKENLRVGDRVKAYLLRIDRQGRGPQLILSRTAPEFLVKLFELEVPEIEEGMLEIRAAVRDPGMRAKIAV
KANDARIDPQGTCIGMRGSRVQAVTQELAGERVDIVLWAPEPAQFVINALSPAEVSRIMVDEDNHSMDVIVEEEQLALAI
GRSGQNVKLAAELTGWYLNIMTVAEAEEKHQAEDAALRQLFVSALSVDEDTAAKLVEEGFAALEEVAYVPLEEMLEIEGF
NEELVGELRSRARDALLTQAIASEEQVENVADDLKDVEGLDAELVRKLAAGGVTTRDDLADLAVDDLVDMTGMEAEAARA
IIMKAREHWFNQ

Sequences:

>Translated_492_residues
MSREILLLVDALASEKNVSKDVVFSALEMALASATKKKFTDDEMDVRVEIDRHTGQYQTFRRWLVVEDELLESESKEIGL
IDARDRDPRIELGAFIEEPMEAVEFGRIGAQTAKQVILQRIRDAEREQLLNEFLQRREHLVIGTIKRIERGNAIVECGKL
EAVLPRDQMIPKENLRVGDRVKAYLLRIDRQGRGPQLILSRTAPEFLVKLFELEVPEIEEGMLEIRAAVRDPGMRAKIAV
KANDARIDPQGTCIGMRGSRVQAVTQELAGERVDIVLWAPEPAQFVINALSPAEVSRIMVDEDNHSMDVIVEEEQLALAI
GRSGQNVKLAAELTGWYLNIMTVAEAEEKHQAEDAALRQLFVSALSVDEDTAAKLVEEGFAALEEVAYVPLEEMLEIEGF
NEELVGELRSRARDALLTQAIASEEQVENVADDLKDVEGLDAELVRKLAAGGVTTRDDLADLAVDDLVDMTGMEAEAARA
IIMKAREHWFNQ
>Mature_491_residues
SREILLLVDALASEKNVSKDVVFSALEMALASATKKKFTDDEMDVRVEIDRHTGQYQTFRRWLVVEDELLESESKEIGLI
DARDRDPRIELGAFIEEPMEAVEFGRIGAQTAKQVILQRIRDAEREQLLNEFLQRREHLVIGTIKRIERGNAIVECGKLE
AVLPRDQMIPKENLRVGDRVKAYLLRIDRQGRGPQLILSRTAPEFLVKLFELEVPEIEEGMLEIRAAVRDPGMRAKIAVK
ANDARIDPQGTCIGMRGSRVQAVTQELAGERVDIVLWAPEPAQFVINALSPAEVSRIMVDEDNHSMDVIVEEEQLALAIG
RSGQNVKLAAELTGWYLNIMTVAEAEEKHQAEDAALRQLFVSALSVDEDTAAKLVEEGFAALEEVAYVPLEEMLEIEGFN
EELVGELRSRARDALLTQAIASEEQVENVADDLKDVEGLDAELVRKLAAGGVTTRDDLADLAVDDLVDMTGMEAEAARAI
IMKAREHWFNQ

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=494, Percent_Identity=52.834008097166, Blast_Score=499, Evalue=1e-142,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 55014; Mature: 54883

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREILLLVDALASEKNVSKDVVFSALEMALASATKKKFTDDEMDVRVEIDRHTGQYQTF
CCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHH
RRWLVVEDELLESESKEIGLIDARDRDPRIELGAFIEEPMEAVEFGRIGAQTAKQVILQR
HHHHEECHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
IRDAEREQLLNEFLQRREHLVIGTIKRIERGNAIVECGKLEAVLPRDQMIPKENLRVGDR
HHHHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCEEEECCCCEECCHHHCCCCCCCCCHHH
VKAYLLRIDRQGRGPQLILSRTAPEFLVKLFELEVPEIEEGMLEIRAAVRDPGMRAKIAV
HHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEE
KANDARIDPQGTCIGMRGSRVQAVTQELAGERVDIVLWAPEPAQFVINALSPAEVSRIMV
EECCCEECCCCCEECCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCHHHHHEEEE
DEDNHSMDVIVEEEQLALAIGRSGQNVKLAAELTGWYLNIMTVAEAEEKHQAEDAALRQL
ECCCCCEEEEEECCCEEEEECCCCCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHH
FVSALSVDEDTAAKLVEEGFAALEEVAYVPLEEMLEIEGFNEELVGELRSRARDALLTQA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
IASEEQVENVADDLKDVEGLDAELVRKLAAGGVTTRDDLADLAVDDLVDMTGMEAEAARA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
IIMKAREHWFNQ
HHHHHHHHHCCC
>Mature Secondary Structure 
SREILLLVDALASEKNVSKDVVFSALEMALASATKKKFTDDEMDVRVEIDRHTGQYQTF
CCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHH
RRWLVVEDELLESESKEIGLIDARDRDPRIELGAFIEEPMEAVEFGRIGAQTAKQVILQR
HHHHEECHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
IRDAEREQLLNEFLQRREHLVIGTIKRIERGNAIVECGKLEAVLPRDQMIPKENLRVGDR
HHHHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCEEEECCCCEECCHHHCCCCCCCCCHHH
VKAYLLRIDRQGRGPQLILSRTAPEFLVKLFELEVPEIEEGMLEIRAAVRDPGMRAKIAV
HHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEE
KANDARIDPQGTCIGMRGSRVQAVTQELAGERVDIVLWAPEPAQFVINALSPAEVSRIMV
EECCCEECCCCCEECCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCHHHHHEEEE
DEDNHSMDVIVEEEQLALAIGRSGQNVKLAAELTGWYLNIMTVAEAEEKHQAEDAALRQL
ECCCCCEEEEEECCCEEEEECCCCCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHH
FVSALSVDEDTAAKLVEEGFAALEEVAYVPLEEMLEIEGFNEELVGELRSRARDALLTQA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
IASEEQVENVADDLKDVEGLDAELVRKLAAGGVTTRDDLADLAVDDLVDMTGMEAEAARA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
IIMKAREHWFNQ
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]