Click here to switch to the map view.

The map label for this gene is dapE

Identifier: 34496911

GI number: 34496911

Start: 1544272

End: 1545405

Strand: Reverse

Name: dapE

Synonym: CV_1456

Alternate gene names: 34496911

Gene position: 1545405-1544272 (Counterclockwise)

Preceding gene: 34496912

Following gene: 34496910

Centisome position: 32.53

GC content: 67.72

Gene sequence:

>1134_bases
ATGAGCGCGACGCTGGATCTCGCCCGCGAGCTGATCCGCCGCGATTCCGTCACCCCGCGCGATGAAGGCTGCCAGGCGCT
GATGATAGAACGCCTGGAAGCGATAGGCTTCAAGGTGGAAAAGATGCGCCACGGCGACGTGGACAACTTCTGGGCGCGCC
GCGGCGACGCCGGCCCGCTGTTCTGCTTCGCCGGCCATACCGACGTGGTGCCCACCGGGCCGCTGGACAAATGGGACAGC
CCGCCGTTCGAGCCGACCGTGCGCAACGGCCTCTTGTACGGCCGCGGCGCCGCCGACATGAAGGCGTCTCTGGCCGCCTT
CGTCACCGCCTGCGAGCGCTTCGTCGCCGAACATCCTGATCACAAGGGCTCGCTGGCGCTGCTGATCACCTCGGACGAGG
AAGGCGTGGCGGTCGACGGCACGGTCAAGGTGGTCGACGCGCTGGAAGCGCGCGGCGAGACGATAGACTACTGCATCGTC
GGCGAGCCGACCTCCGAACAGCGGCTGGGCGACACCGTGAAAAACGGCCGCCGCGGCTCGTTGTCCGGCCGCCTGGTCGT
GCACGGCATCCAGGGCCACATCGCCTACCCGCAGCTGGCGAAAAACCCCATCCACCTGATGGCGCCGGCCTTGGCGGAAC
TGGCCGCCACCCGCTGGGACGAGGGCAATGCCTTTTTCCCGCCCACCAGCTGGCAGGTGTCCAACATCCAGGCCGGCACC
GGCGCCACCAACGTCATCCCAGGCCACTGCGAGCTGCTGTTCAACTTCCGCTTCTCGCCGGAAAGCACCGCGGAAAGCCT
GAAAGAACGGGTGTATCAGATACTGGACAAGCACGGTCTGGGCTACGAGCTGCACTGGCAGCTGTCCGGCCAGCCCTTCA
TCACGCCGCCGGGCGCGCTGACCGACGCGCTCAGCGCGGCCATCGCCGAGGTGTCGGGCGCCAAGGCCGAACTGTCCACC
ACCGGCGGCACCTCGGACGGCCGCTTCATCAAGCGCATCGCGCGCGAGCTGGTGGAATTCGGCCCCATCAACGCCACCAT
CCACAAGCTGAACGAATGCGTGGAAGTGGCCGACGTGGAGCCGCTGGCCGCCATCTACCGCCGCACGCTGGAGGGGCTGC
TGGCCAAGGCATGA

Upstream 100 bases:

>100_bases
TGCTGGACAAGCCGTCGGTGATCAAGCGCCCGGTGCTGGAATGGGACGGCAAGGTCGGCGTCGGCTTCGCCGAAGCCGAC
TGGGCGGAGCGATTCGGCAA

Downstream 100 bases:

>100_bases
GGGCCTGCGCTCCGCTATGCTGAAACCGGCGCCCGCGCCGGTTTTTTCACATCCACGCCGCGTCCCTGGCGCAACCATGG
TTTTTCGCGTCTCCACGCCG

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSATLDLARELIRRDSVTPRDEGCQALMIERLEAIGFKVEKMRHGDVDNFWARRGDAGPLFCFAGHTDVVPTGPLDKWDS
PPFEPTVRNGLLYGRGAADMKASLAAFVTACERFVAEHPDHKGSLALLITSDEEGVAVDGTVKVVDALEARGETIDYCIV
GEPTSEQRLGDTVKNGRRGSLSGRLVVHGIQGHIAYPQLAKNPIHLMAPALAELAATRWDEGNAFFPPTSWQVSNIQAGT
GATNVIPGHCELLFNFRFSPESTAESLKERVYQILDKHGLGYELHWQLSGQPFITPPGALTDALSAAIAEVSGAKAELST
TGGTSDGRFIKRIARELVEFGPINATIHKLNECVEVADVEPLAAIYRRTLEGLLAKA

Sequences:

>Translated_377_residues
MSATLDLARELIRRDSVTPRDEGCQALMIERLEAIGFKVEKMRHGDVDNFWARRGDAGPLFCFAGHTDVVPTGPLDKWDS
PPFEPTVRNGLLYGRGAADMKASLAAFVTACERFVAEHPDHKGSLALLITSDEEGVAVDGTVKVVDALEARGETIDYCIV
GEPTSEQRLGDTVKNGRRGSLSGRLVVHGIQGHIAYPQLAKNPIHLMAPALAELAATRWDEGNAFFPPTSWQVSNIQAGT
GATNVIPGHCELLFNFRFSPESTAESLKERVYQILDKHGLGYELHWQLSGQPFITPPGALTDALSAAIAEVSGAKAELST
TGGTSDGRFIKRIARELVEFGPINATIHKLNECVEVADVEPLAAIYRRTLEGLLAKA
>Mature_376_residues
SATLDLARELIRRDSVTPRDEGCQALMIERLEAIGFKVEKMRHGDVDNFWARRGDAGPLFCFAGHTDVVPTGPLDKWDSP
PFEPTVRNGLLYGRGAADMKASLAAFVTACERFVAEHPDHKGSLALLITSDEEGVAVDGTVKVVDALEARGETIDYCIVG
EPTSEQRLGDTVKNGRRGSLSGRLVVHGIQGHIAYPQLAKNPIHLMAPALAELAATRWDEGNAFFPPTSWQVSNIQAGTG
ATNVIPGHCELLFNFRFSPESTAESLKERVYQILDKHGLGYELHWQLSGQPFITPPGALTDALSAAIAEVSGAKAELSTT
GGTSDGRFIKRIARELVEFGPINATIHKLNECVEVADVEPLAAIYRRTLEGLLAKA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=372, Percent_Identity=60.2150537634409, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1790395, Length=382, Percent_Identity=26.4397905759162, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1789236, Length=223, Percent_Identity=29.1479820627803, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17551016, Length=257, Percent_Identity=28.4046692607004, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_CHRVO (Q7NY19)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901126.1
- ProteinModelPortal:   Q7NY19
- SMR:   Q7NY19
- MEROPS:   M20.010
- GeneID:   2550421
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1456
- NMPDR:   fig|243365.1.peg.1456
- HOGENOM:   HBG728841
- OMA:   ARNPVHQ
- PhylomeDB:   Q7NY19
- ProtClustDB:   PRK13009
- BioCyc:   CVIO243365:CV_1456-MONOMER
- HAMAP:   MF_01690
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 40806; Mature: 40674

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 68-68 ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATLDLARELIRRDSVTPRDEGCQALMIERLEAIGFKVEKMRHGDVDNFWARRGDAGPL
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHCCCCCCE
FCFAGHTDVVPTGPLDKWDSPPFEPTVRNGLLYGRGAADMKASLAAFVTACERFVAEHPD
EEECCCCCCCCCCCCCCCCCCCCCHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
HKGSLALLITSDEEGVAVDGTVKVVDALEARGETIDYCIVGEPTSEQRLGDTVKNGRRGS
CCCCEEEEEECCCCCEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCC
LSGRLVVHGIQGHIAYPQLAKNPIHLMAPALAELAATRWDEGNAFFPPTSWQVSNIQAGT
CCCEEEEEECCCCEECHHHHCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCEECCEECCC
GATNVIPGHCELLFNFRFSPESTAESLKERVYQILDKHGLGYELHWQLSGQPFITPPGAL
CCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEECCCCHH
TDALSAAIAEVSGAKAELSTTGGTSDGRFIKRIARELVEFGPINATIHKLNECVEVADVE
HHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
PLAAIYRRTLEGLLAKA
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SATLDLARELIRRDSVTPRDEGCQALMIERLEAIGFKVEKMRHGDVDNFWARRGDAGPL
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHCCCCCCE
FCFAGHTDVVPTGPLDKWDSPPFEPTVRNGLLYGRGAADMKASLAAFVTACERFVAEHPD
EEECCCCCCCCCCCCCCCCCCCCCHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
HKGSLALLITSDEEGVAVDGTVKVVDALEARGETIDYCIVGEPTSEQRLGDTVKNGRRGS
CCCCEEEEEECCCCCEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCC
LSGRLVVHGIQGHIAYPQLAKNPIHLMAPALAELAATRWDEGNAFFPPTSWQVSNIQAGT
CCCEEEEEECCCCEECHHHHCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCEECCEECCC
GATNVIPGHCELLFNFRFSPESTAESLKERVYQILDKHGLGYELHWQLSGQPFITPPGAL
CCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEECCCCHH
TDALSAAIAEVSGAKAELSTTGGTSDGRFIKRIARELVEFGPINATIHKLNECVEVADVE
HHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
PLAAIYRRTLEGLLAKA
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782