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The map label for this gene is puuA [H]

Identifier: 34496903

GI number: 34496903

Start: 1535950

End: 1537338

Strand: Direct

Name: puuA [H]

Synonym: CV_1448

Alternate gene names: 34496903

Gene position: 1535950-1537338 (Clockwise)

Preceding gene: 34496900

Following gene: 34496905

Centisome position: 32.33

GC content: 64.94

Gene sequence:

>1389_bases
ATGCCCGCAGGCTGGGAAGCTCGGGCAAACACAGCTGACAGAGGATTTATGGACAGTGCCCTTATCATCAACCAATGGCT
GGACGAACACCATATCACCGAGGTGGAATGTCTGATCCCGGACATGACCGGCCTCGCCCGCGGCAAGATCGTGCCGCGCC
ACAAGTACGACCCCGCCGTCGGATTGCGCCTGCCTGAGGCGGTGCTGTCGATGACGGTGACCGGCGACTACCCCGACCGC
GACTTCACCTCCGTCGCCGACCCCGACATGAAGCTGCTGCCCGATCTGAGCACCTTGCGCCCGGTGCCGTGGAACAAGGA
GCCCACCGCGCAGATCATCCATGATTGCTATTCCTTCGCCGGCGACGCGGTGGACCTGGCGCCGCGCAACGTGCTCAAGC
GCATCCTGAAACTGTACGAGGACCAGGGTTGGAAGCCGGTGATCGCGCCGGAGATGGAATTCTACCTGGTGGAGATCCAG
CCGGACGAGGACTTGCCGCTGCGCCCGCCGGTGGGCCGCACCCGCCGCACCGAGGCGGGGATGCAGAGCTTTTCCATCGA
CGCGGTCAACGAATACGACGACATCTTCGACGTGATGTACGACTGGTGCGAGGCCCAGGGCCTGGCGCTGGACACGCTGA
TCCACGAGATGGGCACCGCGCAGATGGAGATCAACCTGGACCACGGCGATCCGCTGCAACTGGCCGACCAGGTGTTCCTG
TTCAAGCGCACCGTGCGCGAGGTGGCGATCCGCAACAATATGTACGCCACCTTCATGGCCAAGCCGATGCAGGGCCAGCC
CGGCAGCGCGATGCACATGCACCAGAGCGTGGTGGACATCGCCAGCGGCGGCAACATCTTCAGCCTGGACGATGGCCAGC
CGTCTGAGGCCTTCCTGCACTTCATCGGCGGCCTGCAGCGCTATCTGCCGGCGGCGATGCCGTTCTTCGCGCCCTACGTC
AATTCCTACCGCCGCCTCAGCCGCTACACCTCGGCGCCGATCAATCTCAGTTGGGGCTACGACAACCGTACCTGCGGTTT
GAGGGTGCCGCACTCCGATCCGGAGGCGAGGCGGGTGGAGAACCGGCTGGCCGGTGTGGACGTCAATCCTTACCTGGCGA
TGGCGGCCAGCCTGGCCTGCGGCTACCTGGGCATGCGCGACCGCATCGATCCGTCCGAGCCTTTGTCCGGCAGCGCCTAC
GAGCAGCCCCATCAGTTGCCGCGCCACCTGGACGACGCCATCGACATGCTGCTGCGCTGCCGGCCGCTATCCGAGCTGTT
CGGCGAGCACTTCATCGAGACCTACGCCGCGATCAAGGAGGCGGAGTACCGCGAGTACTTCGACGTGATCAGCCCGTGGG
AGCGCCGCTTCCTGCTGCTGCACGTCTAG

Upstream 100 bases:

>100_bases
CGGCCAATCCACTCATTTTCCACTGCGGCCGCCAGGCCGCTGCTCGCGGACCGCAGCCTGTTCTTATTGCAGGCCGCGCG
CCGCCATACCCTGACCCATC

Downstream 100 bases:

>100_bases
CCGTACCGGGCCTTCCCGCCCTGCGGGCTGGAAGGCGGCTCTGTGCCAGAAGTCGGCAGCGTGTCCGGCTGGGGCAGGGA
CAGGGGGTTGTCCAATCCGG

Product: glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MPAGWEARANTADRGFMDSALIINQWLDEHHITEVECLIPDMTGLARGKIVPRHKYDPAVGLRLPEAVLSMTVTGDYPDR
DFTSVADPDMKLLPDLSTLRPVPWNKEPTAQIIHDCYSFAGDAVDLAPRNVLKRILKLYEDQGWKPVIAPEMEFYLVEIQ
PDEDLPLRPPVGRTRRTEAGMQSFSIDAVNEYDDIFDVMYDWCEAQGLALDTLIHEMGTAQMEINLDHGDPLQLADQVFL
FKRTVREVAIRNNMYATFMAKPMQGQPGSAMHMHQSVVDIASGGNIFSLDDGQPSEAFLHFIGGLQRYLPAAMPFFAPYV
NSYRRLSRYTSAPINLSWGYDNRTCGLRVPHSDPEARRVENRLAGVDVNPYLAMAASLACGYLGMRDRIDPSEPLSGSAY
EQPHQLPRHLDDAIDMLLRCRPLSELFGEHFIETYAAIKEAEYREYFDVISPWERRFLLLHV

Sequences:

>Translated_462_residues
MPAGWEARANTADRGFMDSALIINQWLDEHHITEVECLIPDMTGLARGKIVPRHKYDPAVGLRLPEAVLSMTVTGDYPDR
DFTSVADPDMKLLPDLSTLRPVPWNKEPTAQIIHDCYSFAGDAVDLAPRNVLKRILKLYEDQGWKPVIAPEMEFYLVEIQ
PDEDLPLRPPVGRTRRTEAGMQSFSIDAVNEYDDIFDVMYDWCEAQGLALDTLIHEMGTAQMEINLDHGDPLQLADQVFL
FKRTVREVAIRNNMYATFMAKPMQGQPGSAMHMHQSVVDIASGGNIFSLDDGQPSEAFLHFIGGLQRYLPAAMPFFAPYV
NSYRRLSRYTSAPINLSWGYDNRTCGLRVPHSDPEARRVENRLAGVDVNPYLAMAASLACGYLGMRDRIDPSEPLSGSAY
EQPHQLPRHLDDAIDMLLRCRPLSELFGEHFIETYAAIKEAEYREYFDVISPWERRFLLLHV
>Mature_461_residues
PAGWEARANTADRGFMDSALIINQWLDEHHITEVECLIPDMTGLARGKIVPRHKYDPAVGLRLPEAVLSMTVTGDYPDRD
FTSVADPDMKLLPDLSTLRPVPWNKEPTAQIIHDCYSFAGDAVDLAPRNVLKRILKLYEDQGWKPVIAPEMEFYLVEIQP
DEDLPLRPPVGRTRRTEAGMQSFSIDAVNEYDDIFDVMYDWCEAQGLALDTLIHEMGTAQMEINLDHGDPLQLADQVFLF
KRTVREVAIRNNMYATFMAKPMQGQPGSAMHMHQSVVDIASGGNIFSLDDGQPSEAFLHFIGGLQRYLPAAMPFFAPYVN
SYRRLSRYTSAPINLSWGYDNRTCGLRVPHSDPEARRVENRLAGVDVNPYLAMAASLACGYLGMRDRIDPSEPLSGSAYE
QPHQLPRHLDDAIDMLLRCRPLSELFGEHFIETYAAIKEAEYREYFDVISPWERRFLLLHV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=441, Percent_Identity=25.3968253968254, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI87081870, Length=446, Percent_Identity=32.2869955156951, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI1790301, Length=458, Percent_Identity=27.7292576419214, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 52378; Mature: 52247

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAGWEARANTADRGFMDSALIINQWLDEHHITEVECLIPDMTGLARGKIVPRHKYDPAV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCCCCCCCCCCCC
GLRLPEAVLSMTVTGDYPDRDFTSVADPDMKLLPDLSTLRPVPWNKEPTAQIIHDCYSFA
CCCCCHHHHEEEEECCCCCCCCCCCCCCCHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHC
GDAVDLAPRNVLKRILKLYEDQGWKPVIAPEMEFYLVEIQPDEDLPLRPPVGRTRRTEAG
CCCHHHCHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHH
MQSFSIDAVNEYDDIFDVMYDWCEAQGLALDTLIHEMGTAQMEINLDHGDPLQLADQVFL
HHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
FKRTVREVAIRNNMYATFMAKPMQGQPGSAMHMHQSVVDIASGGNIFSLDDGQPSEAFLH
HHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHH
FIGGLQRYLPAAMPFFAPYVNSYRRLSRYTSAPINLSWGYDNRTCGLRVPHSDPEARRVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCHHHHHHH
NRLAGVDVNPYLAMAASLACGYLGMRDRIDPSEPLSGSAYEQPHQLPRHLDDAIDMLLRC
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
RPLSELFGEHFIETYAAIKEAEYREYFDVISPWERRFLLLHV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEC
>Mature Secondary Structure 
PAGWEARANTADRGFMDSALIINQWLDEHHITEVECLIPDMTGLARGKIVPRHKYDPAV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCCCCCCCCCCCC
GLRLPEAVLSMTVTGDYPDRDFTSVADPDMKLLPDLSTLRPVPWNKEPTAQIIHDCYSFA
CCCCCHHHHEEEEECCCCCCCCCCCCCCCHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHC
GDAVDLAPRNVLKRILKLYEDQGWKPVIAPEMEFYLVEIQPDEDLPLRPPVGRTRRTEAG
CCCHHHCHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHH
MQSFSIDAVNEYDDIFDVMYDWCEAQGLALDTLIHEMGTAQMEINLDHGDPLQLADQVFL
HHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
FKRTVREVAIRNNMYATFMAKPMQGQPGSAMHMHQSVVDIASGGNIFSLDDGQPSEAFLH
HHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHH
FIGGLQRYLPAAMPFFAPYVNSYRRLSRYTSAPINLSWGYDNRTCGLRVPHSDPEARRVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCHHHHHHH
NRLAGVDVNPYLAMAASLACGYLGMRDRIDPSEPLSGSAYEQPHQLPRHLDDAIDMLLRC
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
RPLSELFGEHFIETYAAIKEAEYREYFDVISPWERRFLLLHV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]